Cancer growth and metastasis最新文献_第5页

Repopulation of ovarian cancer cells after chemotherapy. 化疗后卵巢癌细胞的再生。

Cancer growth and metastasis Pub Date : 2013-02-18 DOI: 10.4137/CGM.S11333

Carlos M Telleria

引用次数: 18

Synergistic lethality of mifepristone and LY294002 in ovarian cancer cells. 米非司酮与LY294002对卵巢癌细胞的协同杀伤作用。

Cancer growth and metastasis Pub Date : 2013-01-01 Epub Date: 2012-01-28 DOI: 10.4137/CGM.S11124

Stacy L Wempe, Carlos D Gamarra-Luques, Carlos M Telleria

{"title":"Synergistic lethality of mifepristone and LY294002 in ovarian cancer cells.","authors":"Stacy L Wempe, Carlos D Gamarra-Luques, Carlos M Telleria","doi":"10.4137/CGM.S11124","DOIUrl":"https://doi.org/10.4137/CGM.S11124","url":null,"abstract":"We have previously shown that the antiprogestin and antiglucocorticoid mifepristone inhibits the growth of ovarian cancer cells. In this work, we hypothesized that cellular stress caused by mifepristone is limited to cytostasis and that cell killing is avoided as a consequence of the persistent activity of the PI3K/Akt survival pathway.To investigate the role of this pathway in mifepristone-induced growth inhibition, human ovarian cancer cells of various histological subtypes and genetic backgrounds were exposed to cytostatic doses of mifepristone in the presence or absence of the PI3K inhibitor, LY294002. The activation of Akt in ovarian cancer cells, as marked by its phosphorylation on Ser473, was not modified by cytostatic concentrations of mifepristone, but it was blocked upon treatment with LY294002. The combination mifepristone/LY294002, but not the individual drugs, killed ovarian cancer cells via apoptosis, as attested by genomic DNA fragmentation and cleavage of caspase-3, and the concomitant down-regulation of anti-apoptotic proteins Bcl-2 and XIAP. From a pharmacological standpoint, when assessing cell growth inhibition using a median-dose analysis algorithm, the interaction between mifepristone and LY294002 was synergistic. The lethality caused by the combination mifepristone/LY294004 in two dimensional cell cultures was recapitulated in organized, tri-dimensional spheroids. This study demonstrates that mifepristone and LY294002, when used individually, cause cell growth arrest, yet when combined, they cause lethality.","PeriodicalId":88440,"journal":{"name":"Cancer growth and metastasis","volume":"6 ","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/CGM.S11124","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31248479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

BITC Sensitizes Pancreatic Adenocarcinomas to TRAIL-induced Apoptosis. BITC使胰腺腺癌对trail诱导的细胞凋亡敏感。

Cancer growth and metastasis Pub Date : 2010-01-20

Christina A Wicker, Ravi P Sahu, Kashmira Kulkarni-Datar, Sanjay K Srivastava, Thomas L Brown

引用次数: 0

BITC Sensitizes Pancreatic Adenocarcinomas to TRAIL-induced Apoptosis. BITC使胰腺腺癌对trail诱导的细胞凋亡敏感。

Cancer growth and metastasis Pub Date : 2010-01-01 DOI: 10.4137/CGM.S3982

C. A. Wicker, R. Sahu, Kashmira Kulkarni‐Datar, S. Srivastava, T. Brown

引用次数: 28

Molecular Imaging of Proteases in Cancer. 癌症中蛋白酶的分子影像学研究。

Cancer growth and metastasis Pub Date : 2009-08-17 DOI: 10.4137/cgm.s2814

Yunan Yang, Hao Hong, Yin Zhang, Weibo Cai

{"title":"Molecular Imaging of Proteases in Cancer.","authors":"Yunan Yang, Hao Hong, Yin Zhang, Weibo Cai","doi":"10.4137/cgm.s2814","DOIUrl":"https://doi.org/10.4137/cgm.s2814","url":null,"abstract":"Proteases play important roles during tumor angiogenesis, invasion, and metastasis. Various molecular imaging techniques have been employed for protease imaging: optical (both fluorescence and bioluminescence), magnetic resonance imaging (MRI), single-photon emission computed tomography (SPECT), and positron emission tomography (PET). In this review, we will summarize the current status of imaging proteases in cancer with these techniques. Optical imaging of proteases, in particular with fluorescence, is the most intensively validated and many of the imaging probes are already commercially available. It is generally agreed that the use of activatable probes is the most accurate and appropriate means for measuring protease activity. Molecular imaging of proteases with other techniques (i.e. MRI, SPECT, and PET) has not been well-documented in the literature which certainly deserves much future effort. Optical imaging and molecular MRI of protease activity has very limited potential for clinical investigation. PET/SPECT imaging is suitable for clinical investigation; however the optimal probes for PET/SPECT imaging of proteases in cancer have yet to be developed. Successful development of protease imaging probes with optimal in vivo stability, tumor targeting efficacy, and desirable pharmacokinetics for clinical translation will eventually improve cancer patient management. Not limited to cancer, these protease-targeted imaging probes will also have broad applications in other diseases such as arthritis, atherosclerosis, and myocardial infarction.","PeriodicalId":88440,"journal":{"name":"Cancer growth and metastasis","volume":"2 ","pages":"13-27"},"PeriodicalIF":0.0,"publicationDate":"2009-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/cgm.s2814","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28780393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 57

Possible Mitochondria-Associated Enzymatic Role in Non-Hodgkin Lymphoma Residual Disease. 线粒体相关酶在非霍奇金淋巴瘤残留病中的可能作用

Cancer growth and metastasis Pub Date : 2008-11-24 DOI: 10.4137/cgm.s2046

Ian Kusao, David Troelstrup, Bruce Shiramizu

{"title":"Possible Mitochondria-Associated Enzymatic Role in Non-Hodgkin Lymphoma Residual Disease.","authors":"Ian Kusao, David Troelstrup, Bruce Shiramizu","doi":"10.4137/cgm.s2046","DOIUrl":"https://doi.org/10.4137/cgm.s2046","url":null,"abstract":"BACKGROUND: The mechanisms responsible for resistant or recurrent disease in childhood non-Hodgkin lymphoma (NHL) are not yet fully understood. A unique mechanism suggesting the role of the mitochondria as the key energy source responsible for residual cells has been assessed in the clinical setting on specimens from patients on therapy were found to have increased copies of mitochondrial DNA (mtDNA) associated with positive minimal residual disease and/or persistent disease (MRD/PD) status. The potential role of mtDNA in MRD/PD emphasizes queries into the contributions of relevant enzymatic pathways responsible for MRD/PD. This study hypothesized that in an in-vitro model, recovering or residual cells from chemotoxicity will exhibit an increase in both citrate synthase and isocitrate dehydrogenase expression and decrease in succinate dehydrogenase expression. PROCEDURE: Ramos cells (Burkitt lymphoma cell line) were exposed to varying concentrations of doxorubicin and vincristine for 1 hr; and allowing for recovery in culture over a 7-day period. cDNA was extracted on days 1 and 7 of the cell culture period to assess the relative expression of the aforementioned genes. RESULTS: Increase citrate synthase, increase isocitrate dehydrogenase and decrease succinate dehydrogenase expressions were found in recovering Ramos cells. CONCLUSION: Recovering lymphoma cells appear to compensate by regulating enzymatic levels of appropriate genes in the Krebs Cycle suggesting an important role of the mitochondria in the presence of residual cells.","PeriodicalId":88440,"journal":{"name":"Cancer growth and metastasis","volume":"1 ","pages":"3-8"},"PeriodicalIF":0.0,"publicationDate":"2008-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2780363/pdf/nihms125555.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28527387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11