PMC biophysics最新文献

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The debut of PMC Biophysics. PMC生物物理学的首次亮相。
PMC biophysics Pub Date : 2008-11-05 DOI: 10.1186/1757-5036-1-1
Huan-Xiang Zhou
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引用次数: 7
ATR-FTIR spectroscopy detects alterations induced by organotin(IV) carboxylates in MCF-7 cells at sub-cytotoxic/-genotoxic concentrations. ATR-FTIR光谱检测亚细胞毒性/-基因毒性浓度的MCF-7细胞中有机锡(IV)羧酸盐诱导的变化。
PMC biophysics Pub Date : 2008-11-05 DOI: 10.1186/1757-5036-1-3
Muhammad S Ahmad, Bushra Mirza, Mukhtiar Hussain, Muhammad Hanif, Saqib Ali, Michael J Walsh, Francis L Martin
{"title":"ATR-FTIR spectroscopy detects alterations induced by organotin(IV) carboxylates in MCF-7 cells at sub-cytotoxic/-genotoxic concentrations.","authors":"Muhammad S Ahmad,&nbsp;Bushra Mirza,&nbsp;Mukhtiar Hussain,&nbsp;Muhammad Hanif,&nbsp;Saqib Ali,&nbsp;Michael J Walsh,&nbsp;Francis L Martin","doi":"10.1186/1757-5036-1-3","DOIUrl":"https://doi.org/10.1186/1757-5036-1-3","url":null,"abstract":"<p><p> The environmental impact of metal complexes such as organotin(IV) compounds is of increasing concern. Genotoxic effects of organotin(IV) compounds (0.01 mug/ml, 0.1 mug/ml or 1.0 mug/ml) were measured using the alkaline single-cell gel electrophoresis (comet) assay to measure DNA single-strand breaks (SSBs) and the cytokinesis-block micronucleus (CBMN) assay to determine micronucleus formation. Biochemical-cell signatures were also ascertained using attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy. In the comet assay, organotin(IV) carboxylates induced significantly-elevated levels of DNA SSBs. Elevated micronucleus-forming activities were also observed. Following interrogation using ATR-FTIR spectroscopy, infrared spectra in the biomolecular range (900 cm-1 - 1800 cm-1) derived from organotin-treated MCF-7 cells exhibited clear alterations in their biochemical-cell fingerprint compared to control-cell populations following exposures as low as 0.0001 mug/ml. Mono-, di- or tri-organotin(IV) carboxylates (0.1 mug/ml, 1.0 mug/ml or 10.0 mug/ml) were markedly cytotoxic as determined by the clonogenic assay following treatment of MCF-7 cells with >/= 1.0 mug/ml. Our results demonstrate that ATR-FTIR spectroscopy can be applied to detect molecular alterations induced by organotin(IV) compounds at sub-cytotoxic and sub-genotoxic concentrations. This biophysical approach points to a novel means of assessing risk associated with environmental contaminants.PACS codes: 87.15.-v, 87.17.-d, 87.18.-h.</p>","PeriodicalId":88297,"journal":{"name":"PMC biophysics","volume":"1 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2008-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/1757-5036-1-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28098898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
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