The open immunology journal最新文献

Characterization of CD19(+)CD23(+)B2 lymphocytes in the allergic airways of BALB/c mice in response to the inhalation of Aspergillus fumigatus conidia. BALB/c小鼠吸入烟曲霉致敏气道中CD19(+)CD23(+)B2淋巴细胞的特征

The open immunology journal Pub Date : 2012-12-28 DOI: 10.2174/1874226201205010046

Sumit Ghosh, Scott A Hoselton, Jane M Schuh

{"title":"Characterization of CD19(+)CD23(+)B2 lymphocytes in the allergic airways of BALB/c mice in response to the inhalation of Aspergillus fumigatus conidia.","authors":"Sumit Ghosh, Scott A Hoselton, Jane M Schuh","doi":"10.2174/1874226201205010046","DOIUrl":"https://doi.org/10.2174/1874226201205010046","url":null,"abstract":"Fungal sensitization in patients with asthma often indicates an unusual disease course in which traditional asthma treatments have little effect and in which morbidity is particularly severe. Airway hyperresponsiveness (AHR), inflammatory infiltrates, smooth muscle hyperplasia, and irreversible fibrotic remodeling of the bronchial architecture are features of allergic fungal asthma. The systemic production of IgE has long been associated with the immunopathogenesis of allergic asthma; however, the role of B lymphocytes and their products in the response to fungal allergens remains unclear. In the present study, we hypothesize that B lymphocytes are recruited to the allergic lung to impact the allergic response. Using a murine fungal aeroallergen model to mimic the human syndrome, we characterized the B cell population in the lung after fungal challenge and found that CD19(+)CD23(+) B2 lymphocyte numbers are increased in the allergic lung in a dynamic process. IgA, IgG(2a), and IgE were prominent in the serum and bronchoalveolar lavage fluid of allergic animals. It was evident that a tissue-centric production of these antibodies was possible. IgA-, IgG-, and IgE-producing cells from the allergic lung were identified by flow cytometry and immunohistochemistry. This study shows for the first time that CD19(+)CD23(+) B2 lymphocyte numbers change in the lung in a dynamic process after inhalation of fungal conidia and their increase has a significant impact on the Ab production in the pulmonary compartment in the context of fungal allergy.","PeriodicalId":88174,"journal":{"name":"The open immunology journal","volume":"5 ","pages":"46-54"},"PeriodicalIF":0.0,"publicationDate":"2012-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541783/pdf/nihms424060.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31158270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of intestinal dendritic cells in murine norovirus infection. 诺如病毒感染小鼠肠道树突状细胞的表征。

The open immunology journal Pub Date : 2011-01-01 DOI: 10.2174/1874226201104010022

Xiuxu Chen, Daniel Leach, Desiré A Hunter, Daniel Sanfelippo, Erika J Buell, Sarah J Zemple, Mitchell H Grayson

引用次数: 9

Preliminary Evidence of Increased Pain and Elevated Cytokines in Fibromyalgia Patients with Defective Growth Hormone Response to Exercise. 纤维肌痛患者运动后生长激素反应缺陷的疼痛增加和细胞因子升高的初步证据。

The open immunology journal Pub Date : 2010-01-01 DOI: 10.2174/1874226201003010009

Rebecca L Ross, Kim D Jones, Robert M Bennett, Rachel L Ward, Brian J Druker, Lisa J Wood

{"title":"Preliminary Evidence of Increased Pain and Elevated Cytokines in Fibromyalgia Patients with Defective Growth Hormone Response to Exercise.","authors":"Rebecca L Ross, Kim D Jones, Robert M Bennett, Rachel L Ward, Brian J Druker, Lisa J Wood","doi":"10.2174/1874226201003010009","DOIUrl":"https://doi.org/10.2174/1874226201003010009","url":null,"abstract":"Mounting evidence suggests fibromyalgia (FM) symptoms are influenced by dysfunction of the hypothalamic-pituitary-hormonal axes (HPHA) and the immune response system. The predominant FM symptoms of widespread pain, fatigue, sleep disturbance, depression, stiffness and exercise intolerance are related to abnormal levels of growth hormone (GH) and are reminiscent of \"sickness behavior\"; a syndrome initiated by the production of pro-inflammatory cytokines in response to various stressors. Cognizant of the reciprocal relationship between HPHA activity and the immune response system, we hypothesized that serum cytokine levels and FM symptom severity would be higher in FM patients with defective growth hormone response to exhaustive exercise compared to those without. Outpatients with FM (n = 165) underwent a Modified Balke Treadmill Protocol and GH response to exhaustive exercise was measured in peripheral blood samples. Levels of IL-1α, IL-1β, IL-1RA, IL-6, IL-8, IL-10, and TNF-α were measured from stored serum on a subset of 24 participants (12 with and 12 without normal GH response to exhaustive exercise). FM symptom severity was assessed using the Fibromyalgia Impact Questionnaire (FIQ), number of tender points and cumulative myalgic scores. GH dysfunction was associated with increased pain scores on the FIQ (p = 0.024), a greater number of tender points (p = 0.014), higher myalgic scores (p = 0.001) and higher pre-exercise levels of inflammatory cytokines IL-1α (p = 0.021), IL-6 (p = 0.012), and IL-8 (p = 0.004). These results suggest that a defective growth hormone response to exercise may be associated with increased levels of blood cytokines and pain severity in FM patients.","PeriodicalId":88174,"journal":{"name":"The open immunology journal","volume":"3 ","pages":"9-18"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868257/pdf/nihms-196279.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28986479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 57

Exposure to Cigarette Smoke Disrupts CCL20-Mediated Antimicrobial Activity in Respiratory Epithelial Cells. 暴露于香烟烟雾会破坏呼吸上皮细胞中ccl20介导的抗菌活性。

The open immunology journal Pub Date : 2009-01-01 DOI: 10.2174/1874226200902010086

Mardi A Crane-Godreau, Matthew A Maccani, Susan K Eszterhas, Sandra L Warner, James A Jukosky, Steven Fiering

{"title":"Exposure to Cigarette Smoke Disrupts CCL20-Mediated Antimicrobial Activity in Respiratory Epithelial Cells.","authors":"Mardi A Crane-Godreau, Matthew A Maccani, Susan K Eszterhas, Sandra L Warner, James A Jukosky, Steven Fiering","doi":"10.2174/1874226200902010086","DOIUrl":"https://doi.org/10.2174/1874226200902010086","url":null,"abstract":"Cigarette smoke (CS) exposure is known to increase infection rates, but the mechanisms are not well understood. These studies tested the hypothesis that CS exposure would impair antimicrobial activity of apical conditioned media from human airway (BEAS-2B) cultures by reducing induction and release of the antimicrobial peptide CCL20. BEAS-2B cultures were exposed to CS extract and assayed for temporal and physical characteristics of release as well as for antimicrobial activity. E. coli were exposed to Beas-2B-conditioned media (BCM) and subsequent bacterial colonies were enumerated. In time course studies TLR-agonist-induced CCL20 transcription and release were rapid, of short duration and release was consistently targeted to the apical/luminal compartment. Cells treated with CS extract had diminished release of CCL20 under both constitutive and toll-like receptor (TLR) agonist stimulating conditions. Exposure of the cells to CS significantly reduced the antimicrobial activity in BCM and neutralizing antibodies to CCL20 brought antibacterial activity back to baseline levels demonstrating that antimicrobial activity in this culture system was primarily attributable to CCL20. These studies add to the understanding of CCL20 as a mucosal antimicrobial and improve insight into a likely mechanism linking infection to CS exposure.","PeriodicalId":88174,"journal":{"name":"The open immunology journal","volume":"2 ","pages":"86-93"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2788948/pdf/nihms146575.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28554853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Generation of eosinophils from unselected bone marrow progenitors: wild-type, TLR- and eosinophil-deficient mice. 从未选择的骨髓祖细胞产生嗜酸性粒细胞:野生型，TLR-和嗜酸性粒细胞缺陷小鼠。

The open immunology journal Pub Date : 2009-01-01 DOI: 10.2174/1874226200902010163

Kimberly D Dyer, Caroline M Percopo, Helene F Rosenberg

{"title":"Generation of eosinophils from unselected bone marrow progenitors: wild-type, TLR- and eosinophil-deficient mice.","authors":"Kimberly D Dyer, Caroline M Percopo, Helene F Rosenberg","doi":"10.2174/1874226200902010163","DOIUrl":"https://doi.org/10.2174/1874226200902010163","url":null,"abstract":"We have recently devised a culture method that generates large numbers of eosinophils at high purity from unselected BALB/c mouse bone marrow progenitors [Dyer et al., 2008. J. Immunol. 181: 4004-9]. Here we present the extended scope of this approach, as we have used this method successfully to generate eosinophil cultures of virtually 100% purity from bone marrow from C57BL/6 mice, and from TLR2, TLR3, TLR7 and TLR9-gene-deleted mouse strains on the C57BL/6 background. Both wild-type and TLR3 gene-deleted bone marrow eosinophils (bmEos) are functional, releasing peroxidase in response to the secretogogue, platelet activating factor. We have also used this method to re-evaluate production of eosinophils in bone marrow cultures from ΔdblGATA mice, a strain that is eosinophil-deficient in vivo. Interestingly, bmEos can be detected in the ΔdblGATA cultures (5% of total cells at day 10), although ~80-fold fewer bmEos are detected in ΔdblGATA than in parallel wild-type (BALB/c) bone marrow cultures. Overall, we find that generation of large numbers of eosinophils at high purity from unselected bone marrow progenitors proceeds efficiently in a variety of wild-type and gene-deleted strains, and as such this approach shows promise as a universal method for the study of eosinophil structure and function.","PeriodicalId":88174,"journal":{"name":"The open immunology journal","volume":"2 ","pages":"163-167"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2836601/pdf/nihms146913.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28775486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Chemokines and Chemokine Receptors Critical to Host Resistance following Genital Herpes Simplex Virus Type 2 (HSV-2) Infection. 趋化因子和趋化因子受体对生殖器单纯疱疹病毒2型(HSV-2)感染后宿主耐药性至关重要。

The open immunology journal Pub Date : 2008-01-01 DOI: 10.2174/1874226200801010033

M Thapa, D J J Carr

引用次数: 25