Journal of epithelial biology & pharmacology最新文献_第4页

Mucin Glycan Expression is Individual-Specific and Governed by the In Situ Microenvironment 粘蛋白聚糖的表达是个体特异性的，受原位微环境的控制

Journal of epithelial biology & pharmacology Pub Date : 2008-08-28 DOI: 10.2174/1875044300801010054

A. Slomiany, B. Slomiany

{"title":"Mucin Glycan Expression is Individual-Specific and Governed by the In Situ Microenvironment","authors":"A. Slomiany, B. Slomiany","doi":"10.2174/1875044300801010054","DOIUrl":"https://doi.org/10.2174/1875044300801010054","url":null,"abstract":"Mucin glycosylation and individual susceptibility to disease is linked to the in situ microenvironment that de- termines features of the individual- and disease-specific glycans. In tissue culture paradigm, the in situ environment is not recreated and the synthesis of signature glycans vanishes. To evaluate the impact of the in situ-specific gastric epithelial environment on mucin glycan synthesis we investigated gastric mucin collected from 36 individual rats subjected to the same conditions and diet. The secretion collected during 3 hours of gastric perfusion contained from 67.7 to 1096.6 nmol mucin glycans and from 42.0 to 245.8 nmol glycosphingolipids (GSL). We identified four groups, each consisting of 6 animals, that contained O-glycans interacting with apical epithelial mucin receptor comprised of N-acetylgalactosamine (GalNAc), galactose (Gal), N-acetylglucosamine (GlcNAc) and fucose (Fuc ) in the ratio of 1:2:2:1, 1:2:3:1, 1:3:2:1, and 1:4:2:1. The remaining 10 rats produced glycans containing GalNAc, Gal, GlcNAc, Fuc in ratio 1:1:1:1, partially fucosy- lated glycans, and Fuc-free glycans consisting of GalNAc:Gal:GlcNAc in ratio of 1:4:2. The data support our hypothesis that individual-specific-gastric epithelial environment and individual-specific metabolome provide final imprint on the quantity, composition, and termination of mucin O-glycans.","PeriodicalId":88010,"journal":{"name":"Journal of epithelial biology & pharmacology","volume":"1 1","pages":"54-61"},"PeriodicalIF":0.0,"publicationDate":"2008-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68110210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming Growth Factor-β Signaling Strength Determines Target Gene Expression Profile in Human Keratinocytes 转化生长因子-β信号强度决定人角质形成细胞靶基因表达谱

Journal of epithelial biology & pharmacology Pub Date : 2008-08-06 DOI: 10.2174/1875044300801010040

A. Kareddula, E. Zachariah, D. Notterman, M. Reiss

{"title":"Transforming Growth Factor-β Signaling Strength Determines Target Gene Expression Profile in Human Keratinocytes","authors":"A. Kareddula, E. Zachariah, D. Notterman, M. Reiss","doi":"10.2174/1875044300801010040","DOIUrl":"https://doi.org/10.2174/1875044300801010040","url":null,"abstract":"Transforming Growth Factor(TGF ) maintains keratinocyte homeostasis, and induces epithelial-tomesenchymal transition (EMT) in response to tissue injury. To investigate how these two TGF responses might become uncoupled during malignant transformation, we examined the TGF -regulated gene expression programs and cellular responses in human keratinocytes as a function of TGF type I receptor (T R-I) kinase activity and TGF level. The TGF mediated homeostatic gene response program and cellular growth arrest were extremely sensitive to a reduction in receptor kinase activity, while much stronger inhibition of TGF receptor activity was needed to inhibit the tissue injury response gene expression program and EMT. Both endogenous TGF and high exogenous levels of TGF induced the homeostatic response, while only high levels of TGF induced EMT. These results suggest that a reduction in receptor signaling activity may be sufficient for keratinocytes to escape from TGF ’s tumor suppressor function, while higher levels of TGF associated with tumor progression are pro-invasive/metastatic. These findings have important implications for the optimal use of TGF /Smad signaling antagonists as anti-cancer therapeutics.","PeriodicalId":88010,"journal":{"name":"Journal of epithelial biology & pharmacology","volume":"1 1","pages":"40-53"},"PeriodicalIF":0.0,"publicationDate":"2008-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68110120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Activation and Inactivation Mechanisms of Na-HCO3 Cotransporter NBC1 Na-HCO3共转运体NBC1的激活和失活机制

Journal of epithelial biology & pharmacology Pub Date : 2008-06-30 DOI: 10.2174/1875044300801010035

G. Seki, Hideomi Yamada, S. Horita, Masashi Suzuki, T. Sekine, T. Igarashi, T. Fujita

引用次数: 6

Mineralocorticoid Receptor, Heat Shock Proteins and Immunophilins Par- ticipate in the Transmission of the Tubulogenic Signal of Aldosterone 矿化皮质激素受体、热休克蛋白和亲免疫蛋白参与醛固酮小管生成信号的传递

Journal of epithelial biology & pharmacology Pub Date : 2008-06-04 DOI: 10.2174/1875044300801010024

W. Minuth, A. Blattmann, L. Denk, H. Castrop

{"title":"Mineralocorticoid Receptor, Heat Shock Proteins and Immunophilins Par- ticipate in the Transmission of the Tubulogenic Signal of Aldosterone","authors":"W. Minuth, A. Blattmann, L. Denk, H. Castrop","doi":"10.2174/1875044300801010024","DOIUrl":"https://doi.org/10.2174/1875044300801010024","url":null,"abstract":"Aldosterone exhibits a tubulogenic activity on renal stem/progenitor cells. Little information is available about the signaling between the mineralocorticoid receptor (MR) and the proteins in the cytoplasm. To obtain information, renal stem/progenitor cells from neonatal rabbit were cultured at the interface of an artificial polyester interstitium in a perfu- sion container for 13 days. Development was registered with Soybean Agglutinin (SBA). Microscopy revealed that treat- ment with aldosterone (1 x 10 -7 M) resulted in numerous SBA-labeled tubules. Interference with mineralocorticoid recep- tor (MR) and heat shock protein (hsp) 90 using geldanamycin (3,6 x 10 -6 M) or radicicol (1 x 10 -6 M) showed a lack of tu- bule development. Disturbing MR and hsp 70 with quercetin (2 x 10 -4 M) and KNK 437 (1 x 10 -4 M) generated SBA- labeled cell cluster but not structured tubules. Interference with MR and immunophilins by cyclosporin A (2 x 10 -6 M) and rapamycin (2,2 x 10 -9 M) did not influence the generation of numerous SBA-labeled tubules. In contrast, administration of FK 506 (2 x 10 -8 M) resulted in a lack of tubule development. Thus, the signal for development of tubules induced by al- dosterone is mediated by interaction of MR, hsp 90, hsp 70 and immunophilin FKBP 12.","PeriodicalId":88010,"journal":{"name":"Journal of epithelial biology & pharmacology","volume":"1 1","pages":"24-34"},"PeriodicalIF":0.0,"publicationDate":"2008-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68110025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Epithelial Brush Border Proteomics and Associated Cell Dysfunction 上皮刷状边界蛋白质组学和相关细胞功能障碍

Journal of epithelial biology & pharmacology Pub Date : 2008-05-30 DOI: 10.2174/1875044300801010016

Xiangdong Wang, K. Adler

{"title":"Epithelial Brush Border Proteomics and Associated Cell Dysfunction","authors":"Xiangdong Wang, K. Adler","doi":"10.2174/1875044300801010016","DOIUrl":"https://doi.org/10.2174/1875044300801010016","url":null,"abstract":"Functions and structures of epithelial cells vary, depending upon their locations in organs/tissues. Pathophysi- ological responses of epithelia are associated with challenges, severities, durations and diseases. Epithelial proteomics has been used to understand the role of these cells in the pathogenesis of several diseases, and has the potential to identify novel biomarkers for disease prognosis, diagnosis and therapies. Epithelial brush border membranes (BBMs) are involved in digestion, absorption, metabolism, and transport of nutrients and drugs, clearance of and defense against toxins, and ini- tiation of intracellular signaling and maintenance of cellular structural integrity. The present article review proteomic re- search on epithelial brush borders to understand the potential links among the function and disease associations of epithe- lial BBM proteomics. We found that the amount and functional ratio of epithelial cell BBM proteomic profiles obviously vary among species, cell locations, organ functions, research groups, and methodologies utilized. Many of these proteins may be identified and their functions elucidated, probably providing potential new markers for epithelial-associated dis- eases and potential therapeutic targets. There is still a great need for proteomic studies for analysis and comparisons of BBM protein profiles associated with normal physiological and pathophysiological states, organ function and dysfunction, and clinical health and disease.","PeriodicalId":88010,"journal":{"name":"Journal of epithelial biology & pharmacology","volume":"1 1","pages":"16-23"},"PeriodicalIF":0.0,"publicationDate":"2008-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68109934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Interleukin-13 is Expressed in Mouse Kidney Allograft Rejection and Mediates Proliferation of Renal Tubular Epithelium In Vitro 白细胞介素-13在小鼠肾移植排斥反应中的表达及介导肾小管上皮的增殖

Journal of epithelial biology & pharmacology Pub Date : 2008-05-23 DOI: 10.2174/1875044300801010008

R. Thuillier, O. Cheng, R. Mannon

{"title":"Interleukin-13 is Expressed in Mouse Kidney Allograft Rejection and Mediates Proliferation of Renal Tubular Epithelium In Vitro","authors":"R. Thuillier, O. Cheng, R. Mannon","doi":"10.2174/1875044300801010008","DOIUrl":"https://doi.org/10.2174/1875044300801010008","url":null,"abstract":"Kidney transplantation is the preferred therapy for kidney failure. The leading cause of graft loss is chronic al- lograft nephropathy (CAN). We hypothesize that Interleukin-13 (IL-13), protective against acute kidney graft rejection, is involved in CAN. In mouse kidney allografts, we observe after 2 weeks signs of interstitial inflammation progressing to vasculitis. By 6 weeks, CAN is manifest. IL-13 is overexpressed in allografts versus isografts (p<0.01) throughout the post-transplant course. Concomitantly, we detect markers of fibrogenesis and epithelial-mesenchymal transition. To ex- plore this phenomenon, kidney proximal tubular epithelial cells were cultured with IL-13. Within 6 hours, we show in- creased proliferation compared to untreated cells (p<0.01), occurring through activation of IL4R� /JAK3 and Stat6 and blocked by anti-IL-13 monoclonal antibody. This is the first report of IL-13 inducing a specific biological activity in kid- ney epithelial cells with a possible role in the damage control machinery, indicating its potential as a biomarker and thera- peutic target.","PeriodicalId":88010,"journal":{"name":"Journal of epithelial biology & pharmacology","volume":"1 1","pages":"8-15"},"PeriodicalIF":0.0,"publicationDate":"2008-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68109853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrolyte and Fluid Transport in Mesothelial Cells. 间皮细胞中的电解质和液体运输。

Journal of epithelial biology & pharmacology Pub Date : 2008-01-01 DOI: 10.2174/1875044300801010001

Hong-Long Ji, Hong-Guang Nie

引用次数: 0