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LAMinar Flow: Sterol Transport in a Pathogenic Yeast LAMinar Flow：致病酵母中的甾醇运输
Contact Pub Date : 2024-03-06 DOI: 10.1177/25152564241237625
H. L. Choy, Elizabeth A. Gaylord, T. Doering
{"title":"LAMinar Flow: Sterol Transport in a Pathogenic Yeast","authors":"H. L. Choy, Elizabeth A. Gaylord, T. Doering","doi":"10.1177/25152564241237625","DOIUrl":"https://doi.org/10.1177/25152564241237625","url":null,"abstract":"Cryptococcus neoformans is an important fungal pathogen, responsible for over 140,000 deaths per year worldwide. Like other yeasts, C. neoformans relies on ergosterol as its major membrane sterol and carefully regulates its synthesis and distribution. Ergosterol is also targeted by two of the three compound classes currently used to treat cryptococcal infection. We recently reported the discovery and characterization in C. neoformans of a single retrograde ergosterol transporter of the LAM family, Ysp2. Here we review these findings and discuss directions for future research, including the connections between processes that are perturbed by the absence of Ysp2 (which also abrogates cryptococcal virulence) and possible roles for Ysp2 and other, as yet unknown, lipid transport proteins in this organism.","PeriodicalId":87951,"journal":{"name":"Contact","volume":"59 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140077696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protection of Membrane Contact Protein by the Methionine Sulfoxide Reductases 蛋氨酸亚砜还原酶保护膜接触蛋白
Contact Pub Date : 2024-01-01 DOI: 10.1177/25152564231223480
Jung Mi Lim
{"title":"Protection of Membrane Contact Protein by the Methionine Sulfoxide Reductases","authors":"Jung Mi Lim","doi":"10.1177/25152564231223480","DOIUrl":"https://doi.org/10.1177/25152564231223480","url":null,"abstract":"In this News and Views, I discuss our recent publication that established how steroidogenic acute regulatory-related lipid transfer domain-3 (STARD3), a membrane contact protein situated at lysosomal membranes, plays a role in the detoxification of cholesterol hydroperoxide. STARD3's methionine residues can be oxidized to methionine sulfoxide by cholesterol hydroperoxide, after which methionine sulfoxide reductases reduce the methionine sulfoxide residues back to methionine. The reaction also results in the reduction of the cholesterol hydroperoxide to an alcohol. The cyclic oxidation and reduction of methionine residues in STARD3 at membrane contact sites creates a catalytically efficient mechanism for detoxification of cholesterol hydroperoxide during cholesterol transport, thus protecting membrane contact sites and the entire cell against the toxicity of cholesterol hydroperoxide.","PeriodicalId":87951,"journal":{"name":"Contact","volume":"21 s1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139394637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neuroacanthocytosis Syndromes: The Clinical Perspective 神经棘细胞增多症综合征：临床视角
Contact Pub Date : 2023-12-10 DOI: 10.1177/25152564231210339
Ruth H. Walker, K. Peikert, Hans H. Jung, Andreas Hermann, A. Danek
{"title":"Neuroacanthocytosis Syndromes: The Clinical Perspective","authors":"Ruth H. Walker, K. Peikert, Hans H. Jung, Andreas Hermann, A. Danek","doi":"10.1177/25152564231210339","DOIUrl":"https://doi.org/10.1177/25152564231210339","url":null,"abstract":"The two very rare neurodegenerative diseases historically known as the “neuroacanthocytosis syndromes” are due to mutations of either VPS13A or XK. These are phenotypically similar disorders that affect primarily the basal ganglia and hence result in involuntary abnormal movements as well as neuropsychiatric and cognitive alterations. There are other shared features such as abnormalities of red cell membranes which result in acanthocytes, whose relationship to neurodegeneration is not yet known. Recent insights into the functions of these two proteins suggest dysfunction of lipid processing and trafficking at the subcellular level and may provide a mechanism for neuronal dysfunction and death, and potentially a target for therapeutic interventions.","PeriodicalId":87951,"journal":{"name":"Contact","volume":"8 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138584718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Possible Role of VPS13B in the Formation of Golgi-Lipid Droplet Contacts Associating with the ER VPS13B在与内质网相关的高尔基-脂滴接触形成中的可能作用
Contact Pub Date : 2023-01-01 DOI: 10.1177/25152564231195718
Yuanjiao Du, Xuewen Hu, Weiping Chang, Lin Deng, Wei-Ke Ji, Juan Xiong
{"title":"A Possible Role of VPS13B in the Formation of Golgi-Lipid Droplet Contacts Associating with the ER","authors":"Yuanjiao Du, Xuewen Hu, Weiping Chang, Lin Deng, Wei-Ke Ji, Juan Xiong","doi":"10.1177/25152564231195718","DOIUrl":"https://doi.org/10.1177/25152564231195718","url":null,"abstract":"While the physical interactions between the Golgi apparatus (Golgi) and lipid droplets (LDs) have been suggested through system-level imaging, the Golgi-LD membrane contact sites (MCSs) remain largely uncharacterized. Here, we show evidence to support the existence of Golgi-LD MCSs in HEK293 cells. We further suggest that vacuolar protein sorting-associated protein 13B (VPS13B) localizes to and promotes the formation of Golgi-LD contacts upon oleic acid (OA) stimulation using 3D high-resolution microscopy. Depletion of VPS13B moderately affects the formation of Golgi-LD contacts upon OA treatment in addition to the fragmentation of the Golgi. Although cellular functions of VPS13B-mediated contacts are still elusive, these findings may provide a new insight into related diseases caused by loss-of-function mutations of VPS13B.","PeriodicalId":87951,"journal":{"name":"Contact","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135402152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Role for Two-Pore Channel Type 2 (TPC2)-Mediated Regulation of Membrane Contact Sites During Zebrafish Notochord Biogenesis? 双孔通道2型(TPC2)介导的膜接触位点调控在斑马鱼脊索生物发生中的作用?
Contact Pub Date : 2023-01-01 DOI: 10.1177/25152564231211409
Keira L. Rice, Ching Man Chan, Jeffrey J. Kelu, Andrew L. Miller, Sarah E. Webb
{"title":"A Role for Two-Pore Channel Type 2 (TPC2)-Mediated Regulation of Membrane Contact Sites During Zebrafish Notochord Biogenesis?","authors":"Keira L. Rice, Ching Man Chan, Jeffrey J. Kelu, Andrew L. Miller, Sarah E. Webb","doi":"10.1177/25152564231211409","DOIUrl":"https://doi.org/10.1177/25152564231211409","url":null,"abstract":"We have previously shown that in the developing trunk of zebrafish embryos, two-pore channel type 2 (TPC2)-mediated Ca 2+ release from endolysosomes plays a role in the formation of the skeletal slow muscle. In addition, TPC2-mediated Ca 2+ signaling is required for axon extension and the establishment of synchronized activity in the primary motor neurons. Here, we report that TPC2 might also play a role in the development of the notochord of zebrafish embryos. For example, when tpcn2 was knocked down or out, increased numbers of small vacuoles were formed in the inner notochord cells, compared with the single large vacuole in the notochord of control embryos. This abnormal vacuolation was associated with embryos displaying attenuated body axis straightening. We also showed that TPC2 has a distinct pattern of localization in the notochord in embryos at ∼24 hpf. Finally, we conducted RNAseq to identify differentially expressed genes in tpcn2 mutants compared to wild-type controls, and found that those involved in actin filament severing, cellular component morphogenesis, Ca 2+ binding, and structural constituent of cytoskeleton were downregulated in the mutants. Together, our data suggest that TPC2 activity plays a key role in notochord biogenesis in zebrafish embryos.","PeriodicalId":87951,"journal":{"name":"Contact","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135508590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiple Ways to Keep FFAT Under Control! 多种方法保持FFAT在控制之下!
Contact Pub Date : 2022-01-01 DOI: 10.1177/25152564221101219
Suzan Kors, M. Schrader, Joseph L. Costello
{"title":"Multiple Ways to Keep FFAT Under Control!","authors":"Suzan Kors, M. Schrader, Joseph L. Costello","doi":"10.1177/25152564221101219","DOIUrl":"https://doi.org/10.1177/25152564221101219","url":null,"abstract":"Peroxisomes and the ER are closely inter-connected organelles, which collaborate in the metabolism of lipids. In a recent research paper in the Journal of Cell Biology, we describe a novel mechanism by which peroxisome-ER membrane contact sites are regulated, via phosphorylation of the peroxisomal protein ACBD5. We found that the interaction between ACBD5 and the ER protein VAPB, which we have previously shown to form a tether complex at peroxisome-ER contacts, is controlled by phosphorylation of ACBD5 at two different sites of its FFAT motif – the VAPB binding site. We also identify the kinase GSK3-β as being responsible for direct phosphorylation of ACBD5 to negatively regulate interaction with VAPB, leading to reduced peroxisome-ER contacts. In this article we provide additional insights into how this work, in combination with other studies on phosphorylation of VAP interactors, suggests a complex system of both positive and negative regulation of the FFAT motif via phosphorylation.","PeriodicalId":87951,"journal":{"name":"Contact","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89865475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Erratum to “Married at Birth: Regulation of Cellular Fat Metabolism by ER–Lipid Droplet Crosstalk” 《出生时结婚:er -脂滴串扰对细胞脂肪代谢的调节》的勘误
Contact Pub Date : 2020-12-01 DOI: 10.1177/2515256420985798
{"title":"Erratum to “Married at Birth: Regulation of Cellular Fat Metabolism by ER–Lipid Droplet Crosstalk”","authors":"","doi":"10.1177/2515256420985798","DOIUrl":"https://doi.org/10.1177/2515256420985798","url":null,"abstract":"(1) Ugrankar, R., Bowerman, J., Hariri, H., Chandra, M., Chen, K., Bossanyi, M.-F., Datta, S., Rogers, S., Eckert, K.M., Vale, G., et al. (2019). Drosophila Snazarus Regulates a Lipid Droplet Population at Plasma MembraneDroplet Contacts in Adipocytes. Dev. Cell 50, 557–572.e5, doi:10.1016/j.devcel.2019.07.021. (2) Du, X., Zhou, L., Aw, Y.C., Mak, H.Y., Xu, Y., Rae, J., Wang, W., Zadoorian, A., Hancock, S.E., Osborne, B., et al. (2020). ORP5 localizes to ER-lipid droplet contacts and regulates the level of PI(4)P on lipid droplets. J. Cell Biol. 219, doi:10.1083/jcb.201905162.","PeriodicalId":87951,"journal":{"name":"Contact","volume":"179 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80042385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SNAP iN, SNAP oUT—SNAREs at ER-PM Contact Sites 快速进，快速出-在ER-PM联系站点设置陷阱
Contact Pub Date : 2020-12-01 DOI: 10.1177/2515256420979586
Neha Singh, C. Vannier, T. Galli
{"title":"SNAP iN, SNAP oUT—SNAREs at ER-PM Contact Sites","authors":"Neha Singh, C. Vannier, T. Galli","doi":"10.1177/2515256420979586","DOIUrl":"https://doi.org/10.1177/2515256420979586","url":null,"abstract":"Inter-organelle communication is essential for the exchange of cellular content in eukaryotes, particularly at membrane contact sites between the endoplasmic reticulum (ER) and the plasma membrane (PM). Accomplishing this critical task requires close positioning of the involved membranes via tether proteins and associated complexes. One such complex involves the SNAREs Sec22b and Syntaxin 1. Discovered to be interacting at the ER-PM membrane contact site (MCS), Sec22b-Stx1 forms a unique non-fusogenic bridge tethering the two membranes. Contrarily, SNAP25 was shown to be absent from the Sec22b-Stx1 complexes. Two recent studies focused on this interplay of SNARES and Lipid transfer proteins at MCSs. The Longin domain of Sec22b appeared to be the reason behind SNAP25’s exclusion from Sec22b-Stx1 assembly, and inclusion of E-Syts. It was also shown that yeast Sec9p and mammalian SNAP25 regulate ER-PM contact sites via their interaction with LTP OSBP-homologous proteins (ORP/OSH). In this following short review, we will take a closer look at the protein complexes involving SNAREs at MCSs and potential regulation by the Longin domain of Sec22b.","PeriodicalId":87951,"journal":{"name":"Contact","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84358600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Hansenula Polymorpha Vac8: A Vacuolar Membrane Protein Required for Vacuole Inheritance and Nucleus-Vacuole Junction Formation 多态羊草Vac8:液泡遗传和核-液泡连接形成所需的液泡膜蛋白
Contact Pub Date : 2020-11-01 DOI: 10.1177/2515256420974928
Ritika K. Singh, J. Wróblewska, Rinse de Boer, I. J. van der Klei
{"title":"Hansenula Polymorpha Vac8: A Vacuolar Membrane Protein Required for Vacuole Inheritance and Nucleus-Vacuole Junction Formation","authors":"Ritika K. Singh, J. Wróblewska, Rinse de Boer, I. J. van der Klei","doi":"10.1177/2515256420974928","DOIUrl":"https://doi.org/10.1177/2515256420974928","url":null,"abstract":"Saccharomyces cerevisiae Vac8 is a vacuolar membrane protein, which functions in vacuole inheritance and fusion, nucleus-vacuole junctions, autophagy and the cytoplasm-to-vacuole-targeting pathway. Here, we analyzed Vac8 of the yeast Hansenula polymorpha. We show that HpVac8 localizes to the vacuolar membrane and concentrates in patches at nucleus-vacuole junctions. Analysis of a VAC8 deletion strain indicated that HpVac8 is required for vacuole inheritance and the formation of nuclear-vacuole junctions, but not for vacuole fusion. Previously, organelle proteomics resulted in the identification of Vac8 in peroxisomal fractions isolated from H. polymorpha and S. cerevisiae. However, deletion of H. polymorpha VAC8 had no effect on peroxisome biogenesis or peroxisome-vacuole contact sites.","PeriodicalId":87951,"journal":{"name":"Contact","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88733837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Corrigendum to “Nucleus–Plasma Membrane Contact Sites Are Formed During Spermiogenesis in the Acoel Symsagittifera roscoffensis” “在精子发生过程中形成核-质膜接触点”的勘误表
Contact Pub Date : 2020-10-01 DOI: 10.1177/2515256420963468
{"title":"Corrigendum to “Nucleus–Plasma Membrane Contact Sites Are Formed During Spermiogenesis in the Acoel Symsagittifera roscoffensis”","authors":"","doi":"10.1177/2515256420963468","DOIUrl":"https://doi.org/10.1177/2515256420963468","url":null,"abstract":"","PeriodicalId":87951,"journal":{"name":"Contact","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81624216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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