Biocontrol science最新文献_第7页

Isolation and Characterization of the Polyhexamethylene Biguanide Hydrochloride-Resistant Fungus, Purpureocillium lilacinum. 耐聚六亚甲基双胍盐酸盐真菌紫丁香的分离和鉴定。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2020-07-14 DOI: 10.21203/rs.3.rs-41303/v1

Takako Yamamoto, Hiroki Takahashi, Yikelamu Alimu, Y. Kusuya, Kouichi Hosoya, N. Shigemune, S. Nagai, T. Yaguchi

{"title":"Isolation and Characterization of the Polyhexamethylene Biguanide Hydrochloride-Resistant Fungus, Purpureocillium lilacinum.","authors":"Takako Yamamoto, Hiroki Takahashi, Yikelamu Alimu, Y. Kusuya, Kouichi Hosoya, N. Shigemune, S. Nagai, T. Yaguchi","doi":"10.21203/rs.3.rs-41303/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-41303/v1","url":null,"abstract":"We isolated a fungus from a 20% (= 200,000 µg/mL) aqueous solution of polyhexamethylene biguanide hydrochloride (PHMB), a widely used antimicrobial and examined its morphology and drug resistance profile. Based on the sequence of the internal transcribed spacer region of ribosomal DNA, the fungus was identified as Purpureocillium lilacinum. Although the P. lilacinum type and resistant strains showed similar morphology, the latter had extremely low PHMB susceptibility and was able to grow in 20% aqueous solution of PHMB, which eliminated the type strain. The minimum inhibitory concentration (MIC) of PHMB for the resistant strain was significantly higher than that of the type strain and other pathogenic filamentous fungi and yeasts. The susceptibility to antimicrobial agents and antifungal agents other than PHMB was similar to that of the type strain, therefore the drug resistance of the isolate was specific to PHMB. Furthermore, we sequenced the genome of the isolate to predict PHMB resistance-related genes. Despite its high resistance to PHMB, no well-known genes homologous to fungal PHMB-resistant genes were detected in the genome of the resistant strain. In summary, P. lilacinum was found to be significantly more resistant to PHMB than previously reported, via an unidentified mechanism of drug resistance.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"26 3 1","pages":"157-166"},"PeriodicalIF":1.2,"publicationDate":"2020-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48819156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of Low-Temperature Sterilization using Hydrogen Peroxide Gas Containing Peracetic Acid. 含过氧乙酸过氧化氢气体低温灭菌效果评价。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2020-01-01 DOI: 10.4265/bio.25.185

Minoru Noda, Yoshinobu Sakai, Yoshiro Sakaguchi, Nobuya Hayashi

{"title":"Evaluation of Low-Temperature Sterilization using Hydrogen Peroxide Gas Containing Peracetic Acid.","authors":"Minoru Noda, Yoshinobu Sakai, Yoshiro Sakaguchi, Nobuya Hayashi","doi":"10.4265/bio.25.185","DOIUrl":"https://doi.org/10.4265/bio.25.185","url":null,"abstract":"In low-temperature sterilization for the medical field, hydrogen peroxide sterilization is widely used for its safety. However, its low penetrability and residual amount of sterilant are major concerns. Recently, the combination of hydrogen peroxide and peracetic acid has been found to enforce sporicidal effect, with low concentration in hydrogen peroxide. The application of this finding in medical sterilization is still very limited. To elucidate the combination effect, we compare peracetic acid containing hydrogen peroxide gas sterilizer and conventional hydrogen peroxide gas (plasma) sterilizers. The sterilant penetrability was examined in hollow load process challenge devices with inner diameters of 1 and 2 mm and lengths of 1, 2, and 3 m. As a result, peracetic acid containing hydrogen peroxide gas sterilizer demonstrated total inactivation with all diameters and lengths and achieved the highest sterilant penetrability in this study. The amount of residual sterilant on the surface of the sterilized object was 4.2 μg/cm2, which corresponds to half amount of those of conventional hydrogen peroxide gas sterilizers. These results suggest that the addition of peracetic acid to hydrogen peroxide gas sterilizer can enhance sterilization efficiency and safety.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"25 4","pages":"185-191"},"PeriodicalIF":1.2,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38689799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Morphological Changes in Spores during Germination in Bacillus cereus and Bacillus subtilis. 蜡样芽孢杆菌和枯草芽孢杆菌孢子萌发过程中的形态变化。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2020-01-01 DOI: 10.4265/bio.25.203

Takashi Tsugukuni, Naofumi Shigemune, Motokazu Nakayama, Takahisa Miyamoto

引用次数: 3

A New Protocol for the Detection of Sterigmatocystin-producing Aspergillus Section Versicolores Using a High Discrimination Polymerase. 用高分辨聚合酶检测产生异根半胱氨酸曲霉的新方法。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2020-01-01 DOI: 10.4265/bio.25.113

Atsutaka Kubosaki, Naoki Kobayashi, Maiko Watanabe, Tomoya Yoshinari, Kosuke Takatori, Yutaka Kikuchi, Yukiko Hara-Kudo, Jun Terajima, Yoshiko Sugita-Konishi

引用次数: 8

Postbiotics Applications as Infectious Disease Control Agent in Aquaculture. 生物后制剂在水产养殖中作为传染病防治剂的应用。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2020-01-01 DOI: 10.4265/bio.25.1

Chun Yao Ang, Motohiko Sano, Shigeki Dan, Montira Leelakriangsak, Tamrin M Lal

引用次数: 27

A Multifaceted Evaluation on the Penetration Resistance of Protective Clothing Fabrics against Viral Liquid Drops without Pressure. 无压防护服面料抗病毒滴液渗透性能的多方面评价。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2020-01-01 DOI: 10.4265/bio.25.9

Noriko Shimasaki, Akira Okaue, Michiko Morimoto, Yukiko Uchida, Tomoko Koshiba, Kaoru Tsunoda, Soichi Arakawa, Katsuaki Shinohara

{"title":"A Multifaceted Evaluation on the Penetration Resistance of Protective Clothing Fabrics against Viral Liquid Drops without Pressure.","authors":"Noriko Shimasaki, Akira Okaue, Michiko Morimoto, Yukiko Uchida, Tomoko Koshiba, Kaoru Tsunoda, Soichi Arakawa, Katsuaki Shinohara","doi":"10.4265/bio.25.9","DOIUrl":"https://doi.org/10.4265/bio.25.9","url":null,"abstract":"Healthcare workers should wear appropriate personal protective clothing (PPC) on assuming the risk of exposure to various pathogens. Therefore, it is important to understand PPC performance against pathogen penetration. Currently, standard methods to evaluate and classify the penetration resistance of PPC fabrics with pressure using synthetic blood or phi-X174 phage have been established by the International Organization for Standardization (ISO). However, the penetration of viral liquid drops (VLDrop) on the PPC without pressure is also a major exposure route and more realistic, necessitating further studies. Here, we evaluated the penetration resistance against VLDrop without pressure using phi-X174 phage on woven and nonwoven fabrics of commercially available PPC classified by the ISO, and analyzed in detail the penetration behaviors of VLDrop by quantifying the phage amounts in leak-through and migration into test fabrics. Our results showed that some nonwoven test fabrics had nearly the same penetration resistance against VLDrop, even if the ISO resistance class differed. Furthermore, the results revealed that the amount of leakage through the fabrics was correlated with the migration amount into the fabric, which was related to fluid-repellency of fabrics, suggesting the effectiveness for penetration resistance. Our study may facilitate more appropriate selection for PPC against pathogen penetration.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"25 1","pages":"9-16"},"PeriodicalIF":1.2,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4265/bio.25.9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37739763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Use of Purple Non-Sulfur Photosynthetic Bacteria (Rhodobacter sphaeroides) in Promoting Ciliated Protozoa Growth. 利用紫色无硫光合细菌(球形红杆菌)促进纤毛原生动物生长。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2020-01-01 DOI: 10.4265/bio.25.81

Islam Teiba, Suguru Okunishi, Takeshi Yoshikawa, Makoto Ikenaga, Mohammed Fouad El Basuini, Sheila Mae S Santander-DE Leon, Hiroto Maeda

{"title":"Use of Purple Non-Sulfur Photosynthetic Bacteria (Rhodobacter sphaeroides) in Promoting Ciliated Protozoa Growth.","authors":"Islam Teiba, Suguru Okunishi, Takeshi Yoshikawa, Makoto Ikenaga, Mohammed Fouad El Basuini, Sheila Mae S Santander-DE Leon, Hiroto Maeda","doi":"10.4265/bio.25.81","DOIUrl":"https://doi.org/10.4265/bio.25.81","url":null,"abstract":"Photosynthetic bacterium (PSB) was isolated from sediment samples of Yamagawa Bay, Kagoshima, Japan. Phylogenetic analysis results of PSB isolate were closely related to Rhodobacter sphaeroides, purple non-sulfur photosynthetic bacteria (PNSB). Pink-colored smooth edges of single bacterial colonies were observed after 3-5 days of incubation period on Basic I medium agar plates. Rhodobacter sphaeroides microscopic examination showed a short rod cell (1-2 µm length) with round ends. Sediment and water samples used for ciliates cultivation were collected from Kuwano-ura Bay, Koshiki Island, Japan. Ciliates were cultivated using fish meal with radish leaves medium (MI), with sediment into MI (MII) and algae media (MIII). The use of the algae media (MIII) in cultivation mixture produced the highest total number of ciliates. Big size ciliates were identified as Euplotes minuta and Cyclidium varibonneti, while small size was identified as Micrometopion nutans, based on PCR-DGGE. When ciliates were cultured with the PSB isolate, Rhodobacter sphaeroides as a feed, ciliates grow to 2,081 individual ml-1 72 hrs later. These ﬁndings indicate that PNSB can be used to promote ciliates growth.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"25 2","pages":"81-89"},"PeriodicalIF":1.2,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38017854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Isolation of Aluminum-Tolerant and -Absorbing Yeast. 耐铝吸收酵母的分离。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2020-01-01 DOI: 10.4265/bio.25.231

Kosuke Kakita, Masao Kishida

引用次数: 2

Mitigation of Hyper KCl Stress at 42ºC with Externally Existing Sodium Glutamate to a Halotolerant Brevibacterium sp. JCM 6894. 外源谷氨酸钠对耐盐短杆菌JCM 6894在42℃下的高氯化钾胁迫缓解

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2020-01-01 DOI: 10.4265/bio.25.139

Yue Yin, Haruo Mimura

{"title":"Mitigation of Hyper KCl Stress at 42ºC with Externally Existing Sodium Glutamate to a Halotolerant Brevibacterium sp. JCM 6894.","authors":"Yue Yin, Haruo Mimura","doi":"10.4265/bio.25.139","DOIUrl":"https://doi.org/10.4265/bio.25.139","url":null,"abstract":"Halotolerant Brevibacterium sp. JCM 6894 grew at 37ºC in the presence of 2.3 M KCl, while the growth was repressed with the same concentration of NaCl. When resting cells, 107.4 ± 0.1 (CFU·mL-1), prepared from cells grown in the absence of salts at 30ºC, were exposed to 3.3 M NaCl for 36 h at 42ºC, reduction of the number of resting cells was maintained within a 1-log cycle in the presence of proline, betaine, or ectoine (50 mM). In the presence of 3.3 M KCl, the most functional osmoprotectant was sodium glutamate (50 mM), and the value was 107.2 ± 0.1 (CFU·mL-1) when exposed for 72 h at 42ºC. In the absence of osmoprotectants, the value was reduced to four orders of magnitude in each experimental condition. The number of resting cells, 106.8 ± 0.1 (CFU·mL-1), prepared from grown cells pre-adapted to 2.3 M KCl at 37ºC, was hardly reduced when exposed to 3.3 M KCl in the presence of sodium glutamate more than 50 mM for 72 h at 42ºC. Those results indicate that the isolate can sense the difference in hyper KCl stress as opposed to hyper NaCl stress, and different kinds of osmoadaptation systems can function to cope with each hyper salt stress.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"25 3","pages":"139-147"},"PeriodicalIF":1.2,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38484886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Meat Native Lactic Acid Bacteria Capable to Inhibit Salmonella sp. and Escherichia coli. 能抑制沙门氏菌和大肠杆菌的肉类原生乳酸菌。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2020-01-01 DOI: 10.4265/bio.25.107

Saúl Hernández-Aquino, Ema DE Jesús Maldonado Simán, Luis Alberto Miranda-Romero, Baldomero Alarcón Zuñiga

{"title":"Meat Native Lactic Acid Bacteria Capable to Inhibit Salmonella sp. and Escherichia coli.","authors":"Saúl Hernández-Aquino, Ema DE Jesús Maldonado Simán, Luis Alberto Miranda-Romero, Baldomero Alarcón Zuñiga","doi":"10.4265/bio.25.107","DOIUrl":"https://doi.org/10.4265/bio.25.107","url":null,"abstract":"In this study, lactic acid bacteria (LAB) strains were isolated from ground beef, and it was analyzed if they have any effect on the growth of two reference bacteria (Salmonella sp. and Escherichia coli). It was found that five isolates showed an inhibitory effect in both reference bacteria by spot at the lawn assay. These bacteria were selected to perform growth kinetics in co-culture to determine if they modify the growth parameters of the reference bacteria. Subsequently, LAB cultures and three treatments (crude extract, thermally treated and thermally treated with neutral pH) of cells free supernatants (CFS) were screened by the agar well diffusion assay. In co-culture, selected LAB altered the growth rate and reduce the maximum population of both reference bacteria. While, LAB cultures and CFS also showed antimicrobial activity, and there was no significant difference among CFS treatments. LAB isolated from ground beef showed an antimicrobial effect against the reference bacteria that could be used for meat biopreservation purposes.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"25 2","pages":"107-112"},"PeriodicalIF":1.2,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4265/bio.25.107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38017447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1