发布求助
文献互助 智能选刊 最新文献

Biochemistry and molecular biology international最新文献

筛选
英文 中文
Heat stress affects the glucocorticoid receptor interaction with heat shock protein Hsp70 in the rat liver. 热应激影响大鼠肝脏糖皮质激素受体与热休克蛋白Hsp70的相互作用。
Biochemistry and molecular biology international Pub Date : 1998-09-01 DOI: 10.1080/15216549800203562
A Cvoro, J Dundjerski, D Trajković, G Matić
{"title":"Heat stress affects the glucocorticoid receptor interaction with heat shock protein Hsp70 in the rat liver.","authors":"A Cvoro,&nbsp;J Dundjerski,&nbsp;D Trajković,&nbsp;G Matić","doi":"10.1080/15216549800203562","DOIUrl":"https://doi.org/10.1080/15216549800203562","url":null,"abstract":"<p><p>The association of glucocorticoid hormones receptor (GR) with heat shock protein Hsp70 in the liver cytosol of rats exposed to 41 degrees C whole body hyperthermic stress was examined by quantitative immunoblotting of the two proteins within immunopurified untransformed GR multiprotein complexes. The presence of Hsp70 in the rat liver GR heterocomplexes was confirmed, and 2-fold increase in the Hsp70 relative to the steroid binding protein content within the complexes was recorded 2 and 12 h after the stress. This increase exceeded the stress-induced elevation in the total cytoplasmic Hsp70 level, but could not be seen 24 h after the stress, when cytoplasmic Hsp70 returned to basal level. The results suggest that hyperthermic stress alters the composition of the rat liver untransformed GR heterocomplexes increasing the Hsp70 share.</p>","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"46 1","pages":"63-70"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549800203562","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20697284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Sulphydryl groups and their relation to the antioxidant enzymes of chelonian red blood cells. 巯基及其与龟红细胞抗氧化酶的关系。
Biochemistry and molecular biology international Pub Date : 1998-09-01 DOI: 10.1080/15216549800203652
M A Torsoni, R I Viana, S H Ogo
{"title":"Sulphydryl groups and their relation to the antioxidant enzymes of chelonian red blood cells.","authors":"M A Torsoni,&nbsp;R I Viana,&nbsp;S H Ogo","doi":"10.1080/15216549800203652","DOIUrl":"https://doi.org/10.1080/15216549800203652","url":null,"abstract":"<p><p>Thiol groups of hemoglobin and blood glutathione are higher in Geochelone carbonaria than in Geochelone denticulata. Exposure of stripped hemolysate of both tortoises to terc-butyl hydroperoxide, resulted in a higher ferroheme oxidation of G. denticulata hemoglobin. In this example glutathione reductase and glutathione peroxidase, were not active due to the absence of GSH and NADPH, suggesting that the thiol groups of G. carbonaria hemoglobin act as antioxidant, similar to GSH. In the total hemolysate, however, where the antioxidant enzymes are active, both species showed similar levels of hemoglobin oxidation, suggesting that the protective effect of thiol groups of hemoglobin are less effective for heme protection. The activity of glutathione reductase and glutathione peroxidase was higher in erythrocytes of G. denticulata and the activity of catalase and superoxide dismutase was higher in erythrocytes of G. carbonaria.</p>","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"46 1","pages":"147-56"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549800203652","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20697755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Effects of eicosapentaenoic acid and its 15-hydroperoxy and 15-hydroxy derivatives on glucosamine synthetase activity in rabbit gastric mucosa. 二十碳五烯酸及其15-羟基和15-羟基衍生物对兔胃黏膜葡萄糖胺合成酶活性的影响。
Biochemistry and molecular biology international Pub Date : 1998-09-01 DOI: 10.1080/15216549800203662
T Fujita, S Sakuma, N Yamamoto, Y Fujimoto
{"title":"Effects of eicosapentaenoic acid and its 15-hydroperoxy and 15-hydroxy derivatives on glucosamine synthetase activity in rabbit gastric mucosa.","authors":"T Fujita,&nbsp;S Sakuma,&nbsp;N Yamamoto,&nbsp;Y Fujimoto","doi":"10.1080/15216549800203662","DOIUrl":"https://doi.org/10.1080/15216549800203662","url":null,"abstract":"<p><p>The effects of eicosapentaenoic acid and its 15-hydroperoxy and 15-hydroxy adducts on the activity of glucosamine synthetase, the rate-limiting enzyme of mucus synthesis, in rabbit gastric antral mucosa were examined. 15-Hydroperoxy-eicosapentaenoic acid inhibited the glucosamine synthetase activity at concentrations of 10, 20 and 50 microM. The effect was concentration-dependent, and the concentration required for 50% inhibition was approximately 20 microM. Eicosapentaenoic acid and its 15-hydroxy adduct had no significant effect on the enzyme activity at the same concentration range. The experiment utilizing Fe2+ revealed that the inhibitory effect of 15-hydroperoxyeicosapentaenoic acid on the glucosamine synthetase activity is not due to hydroxy radical which is expected to be formed from the hydroperoxy adduct. These results suggest that 15-hydroperoxyeicosapentaenoic acid has the potential to reduce the synthesis of gastric mucus by inhibiting the glucosamine synthetase activity.</p>","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"46 1","pages":"157-63"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549800203662","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20697756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Inhibitory effects by a submandibular gland extract on luteinizing hormone-stimulated testosterone production by testicular cells. 下颌骨腺提取物对睾丸细胞促黄体激素刺激的睾酮产生的抑制作用。
Biochemistry and molecular biology international Pub Date : 1998-09-01 DOI: 10.1080/15216549800203672
S Koshika, K Izukuri, Y Kato, S Saito, M Hosaka
{"title":"Inhibitory effects by a submandibular gland extract on luteinizing hormone-stimulated testosterone production by testicular cells.","authors":"S Koshika,&nbsp;K Izukuri,&nbsp;Y Kato,&nbsp;S Saito,&nbsp;M Hosaka","doi":"10.1080/15216549800203672","DOIUrl":"https://doi.org/10.1080/15216549800203672","url":null,"abstract":"<p><p>In order to evaluate the role of the submandibular gland (SMG) on testosterone (T) production by the testis, primary cultured testicular cells were prepared from rats that had the submandibular gland surgically ablated (G-) and control (sham operated) rats (S.O.) respectively. The cells were incubated with or without 100 ng/ml luteinizing hormone (LH) and/or SMG extract. The same linear increase in T secretion was shown by both S.O. and G- cells on multi-stimulation with LH for up to 96 hrs. However, while an equivalent response was shown for S.O. cells after a single LH stimulation at 96 hrs, T secretion by the G- cells reached a plateau after 24 hrs. The level at 96 hrs was thus approximate 30% and 33% of those of S.O. cells with and without multi-stimulus by LH for 96 hrs, respectively. When S.O. cells were cultured with SMG extract, LH-stimulated T secretion was dose-dependently inhibited and there was no effect on basal T secretion. The inhibitory effect was abolished by treatment at 95 degrees C for 5 min. Ultra-filtration indicated that the molecular size of the inhibitory agent was greater than 30,000. It is proposed that SMG may contain a high molecular weight, heat labile soluble factor(s) which affects T secretion by inhibiting LH action in testicular cells.</p>","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"46 1","pages":"165-73"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549800203672","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20697757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Insulin rapidly induces nuclear translocation of PI3-kinase in HepG2 cells. 胰岛素快速诱导HepG2细胞中pi3激酶的核易位。
Biochemistry and molecular biology international Pub Date : 1998-09-01 DOI: 10.1080/15216549800203692
S J Kim
{"title":"Insulin rapidly induces nuclear translocation of PI3-kinase in HepG2 cells.","authors":"S J Kim","doi":"10.1080/15216549800203692","DOIUrl":"https://doi.org/10.1080/15216549800203692","url":null,"abstract":"<p><p>Insulin action on nuclear PI3-Kinase and IRS-1 was explored in HepG2 cells. Following insulin treatment, the cells were subjected to subcellular fractionation. Western blot analyses were carried out to identify IRS-1 and PI3-Kinase in the nuclear and postnuclear preparations. IRS-1 protein was identified in the nucleus under basal condition. Insulin had no effect in the content of nuclear IRS-1. In contrast, PI3-Kinase was not detected under basal condition. However, insulin treatment for 1 to 10 min caused significant increase of PI3-Kinase in the nucleus while it induced corresponding decrease of PI3-Kinase in cytoplasm. Strikingly, Insulin stimulated the association of IRS-1 and PI3-Kinase in the nucleus in a similar kinetics with the nuclear translocation of PI3-Kinase. These results suggest that insulin induces nuclear translocation of PI3-Kinase and the translocated PI3-Kinase associates with nuclear IRS-1. The association of IRS-1 and PI3-Kinase in the nucleus in response to insulin may play important roles in nuclear insulin actions.</p>","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"46 1","pages":"187-96"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549800203692","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20697759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 37
Oxygen free-radicals mediate the damaging effect of ultraviolet light on membrane mitochondria. 氧自由基介导紫外线对细胞膜线粒体的破坏作用。
Biochemistry and molecular biology international Pub Date : 1998-09-01 DOI: 10.1080/15216549800203712
E Chávez, C Zazueta, A Cuéllar, H Reyes-Vivas, N García
{"title":"Oxygen free-radicals mediate the damaging effect of ultraviolet light on membrane mitochondria.","authors":"E Chávez,&nbsp;C Zazueta,&nbsp;A Cuéllar,&nbsp;H Reyes-Vivas,&nbsp;N García","doi":"10.1080/15216549800203712","DOIUrl":"https://doi.org/10.1080/15216549800203712","url":null,"abstract":"<p><p>This paper reports evidence that exposure of mitochondria to near-ultraviolet light inhibits electron transport, collapses the electric gradient, and increases non-specific membrane permeability to matrix solutes such as Ca2+. Membrane energization, as well as superoxide dismutase and catalase avoid membrane leakiness. Increased permeability correlates with a diminution in the titrated thiol groups. Plausibly the pore is formed through the formation of sulfhydryl bridges by the action of UV light-derived oxygen-centered free- radicals on membrane proteins.</p>","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"46 1","pages":"207-14"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549800203712","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20697761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
The beta subunit of chloroplast ATP synthase (CF0CF1-ATPase) is phosphorylated by casein kinase II. 叶绿体ATP合成酶(cf0cf1 -ATP酶)的β亚基被酪蛋白激酶II磷酸化。
Biochemistry and molecular biology international Pub Date : 1998-09-01 DOI: 10.1080/15216549800203602
M Kanekatsu, H Saito, K Motohashi, T Hisabori
{"title":"The beta subunit of chloroplast ATP synthase (CF0CF1-ATPase) is phosphorylated by casein kinase II.","authors":"M Kanekatsu,&nbsp;H Saito,&nbsp;K Motohashi,&nbsp;T Hisabori","doi":"10.1080/15216549800203602","DOIUrl":"https://doi.org/10.1080/15216549800203602","url":null,"abstract":"<p><p>We studied a phosphate acceptor for casein kinase II (CK-II) in chloroplasts, and found a 56 kDa protein (p56) as an acceptor, which was partially purified from the stroma of spinach chloroplasts. The N-terminal amino acid sequence of p56 was identical with that of the beta subunit of chloroplast ATP synthase (CF0CF1-ATPase). In addition, the recombinant beta subunit of CF1 was phosphorylated when the subunit was incubated with CK-II. These results suggest that the beta subunit of CF1 is a substrate protein of CK-II in the chloroplast.</p>","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"46 1","pages":"99-105"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549800203602","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20697826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 43
Analysis of glycosphingolipids of human head and neck carcinomas with comparison to normal tissue. 人头颈癌组织鞘糖脂与正常组织的比较分析。
Biochemistry and molecular biology international Pub Date : 1998-09-01 DOI: 10.1080/15216549800203632
G Bolot, M J David, T Taki, S Handa, T Kasama, M Richard, J C Pignat, L Thomas, J Portoukalian
{"title":"Analysis of glycosphingolipids of human head and neck carcinomas with comparison to normal tissue.","authors":"G Bolot,&nbsp;M J David,&nbsp;T Taki,&nbsp;S Handa,&nbsp;T Kasama,&nbsp;M Richard,&nbsp;J C Pignat,&nbsp;L Thomas,&nbsp;J Portoukalian","doi":"10.1080/15216549800203632","DOIUrl":"https://doi.org/10.1080/15216549800203632","url":null,"abstract":"<p><p>Glycosphingolipids of head and neck carcinomas from six tumor-bearing patients were analyzed and compared to those of normal tissue from similar areas. The total glycosphingolipid content and the lipid-bound sialic acid were much higher in carcinomas than in normal tissue. Major neutral glycolipids were glucosylceramide, lactosylceramide, trihexosylceramide and paragloboside. Sulfatides were seen only in extracts from normal tissue which also showed a rather simple ganglioside pattern with #GM3 and GD3 as major species, whereas tumors showed additional species such as GM2 and GD2, along with a strong increase in LM1, GM1, GD1a and GT1b.</p>","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"46 1","pages":"125-35"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549800203632","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20697829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Purification and characterization of a soluble form of lysosome-associated membrane glycoprotein-2 (lamp-2) from rat liver lysosomal contents. 从大鼠肝脏溶酶体内容物中纯化溶酶体相关膜糖蛋白-2（lamp-2）并确定其特征。
Biochemistry and molecular biology international Pub Date : 1998-09-01 DOI: 10.1080/15216549800203702
K Akasaki, H Tsuji
{"title":"Purification and characterization of a soluble form of lysosome-associated membrane glycoprotein-2 (lamp-2) from rat liver lysosomal contents.","authors":"K Akasaki, H Tsuji","doi":"10.1080/15216549800203702","DOIUrl":"10.1080/15216549800203702","url":null,"abstract":"<p><p>Lysosomal membrane of rat liver contains a highly glycosylated protein referred to as lamp-2. Lamp-2 occurs to a significant extent in a soluble fraction of rat liver lysosomes. The soluble form of lamp-2 (SF-lamp-2) was purified to electrophoretic homogeneity. An apparent molecular weight M(r) of SF-lamp-2 on sodium dodecy sulfate-polyacrylamide gel electrophoresis was determined to be 91,000 which is 5,000 less than that of the membranous form of lamp-2 (MF-lamp-2). SF- and MF-lamp-2 were very similar to each other in terms of sialic acid content, NH2-terminal amino acid sequence and isoelectric point. Gel filtration data indicated that native SF-lamp-2 has an M(r) = 360,000. Taken together, SF-lamp-2 forms a tetrameric structure consisting of a homogenous polypeptide lacking a membrane-spanning domain and a cytoplasmic tail near the COOH-terminus.</p>","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"46 1","pages":"197-206"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549800203702","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20697760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Thiamine triphosphatase activity in bovine kidney. 牛肾中硫胺素三磷酸酶的活性。
Biochemistry and molecular biology international Pub Date : 1998-09-01 DOI: 10.1080/15216549800203622
A F Makarchikov, I P Chernikevich
{"title":"Thiamine triphosphatase activity in bovine kidney.","authors":"A F Makarchikov,&nbsp;I P Chernikevich","doi":"10.1080/15216549800203622","DOIUrl":"https://doi.org/10.1080/15216549800203622","url":null,"abstract":"<p><p>Properties of soluble thiamine triphosphatase (ThTPase), adenosine triphosphatase, nucleoside triphosphatase and alkaline phosphatase activities in bovine kidney were compared. ThTPase and the other phosphatases differed clearly in their pH-dependences, K(m) and molecular masses. Apparent K(m) and pH optimum for ThTPase were determined to be 45.5 microM and 8.9, respectively. Molecular mass of the enzyme was 29.1 kDa as estimated by Sephadex G-100 gel filtration. The results obtained show bovine kidney to contain a specific soluble ThTPase, this enzyme being the only one hydrolyzing low concentrations of ThTP.</p>","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"46 1","pages":"115-23"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549800203622","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20697828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信