BioChip Journal最新文献_第8页

Advanced Organotypic In Vitro Model Systems for Host-Microbial Coculture. 宿主微生物共培养的先进器官型体外模型系统。

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2023-05-22 DOI: 10.1007/s13206-023-00103-5

Raehyun Kim

{"title":"Advanced Organotypic In Vitro Model Systems for Host-Microbial Coculture.","authors":"Raehyun Kim","doi":"10.1007/s13206-023-00103-5","DOIUrl":"10.1007/s13206-023-00103-5","url":null,"abstract":"In vitro model systems have been advanced to recapitulate important physiological features of the target organ in vivo more closely than the conventional cell line cultures on a petri dish. The advanced organotypic model systems can be used as a complementary or alternative tool for various testing and screening. Numerous data from germ-free animal studies and genome sequencings of clinical samples indicate that human microbiota is an essential part of the human body, but current in vitro model systems rarely include them, which can be one of the reasons for the discrepancy in the tissue phenotypes and outcome of therapeutic intervention between in vivo and in vitro tissues. A coculture model system with appropriate microbes and host cells may have great potential to bridge the gap between the in vitro model and the in vivo counterpart. However, successfully integrating two species in one system introduces new variables to consider and poses new challenges to overcome. This review aims to provide perspectives on the important factors that should be considered for developing organotypic bacterial coculture models. Recent advances in various organotypic bacterial coculture models are highlighted. Finally, challenges and opportunities in developing organotypic microbial coculture models are also discussed.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":" ","pages":"1-27"},"PeriodicalIF":4.3,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10201494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9720866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Paper-Based Radial Flow Assay Integrated to Portable Isothermal Amplification Chip Platform for Colorimetric Detection of Target DNA. 基于纸张的径向流分析与便携式等温扩增芯片平台集成，用于比色检测目标DNA。

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2023-04-27 DOI: 10.1007/s13206-023-00101-7

Tai-Yong Kim, Sanha Kim, Jae Hwan Jung, Min-Ah Woo

{"title":"Paper-Based Radial Flow Assay Integrated to Portable Isothermal Amplification Chip Platform for Colorimetric Detection of Target DNA.","authors":"Tai-Yong Kim, Sanha Kim, Jae Hwan Jung, Min-Ah Woo","doi":"10.1007/s13206-023-00101-7","DOIUrl":"10.1007/s13206-023-00101-7","url":null,"abstract":"A novel integrated detection system that introduces a paper-chip-based molecular detection strategy into a polydimethylsiloxane (PDMS) microchip and temperature control system was developed for on-site colorimetric detection of DNA. For the paper chip-based detection strategy, a padlock probe DNA (PLP)-mediated rolling circle amplification (RCA) reaction for signal amplification and a radial flow assay according to the Au-probe labeling strategy for visualization were optimized and applied for DNA detection. In the PDMS chip, the reactions for ligation of target-dependent PLP, RCA, and labeling were performed one-step under isothermal temperature in a single chamber, and one drop of the final reaction solution was loaded onto the paper chip to form a radial colorimetric signal. To create an optimal analysis environment, not only the optimization of molecular reactions for DNA detection but also the chamber shape of the PDMS chip and temperature control system were successfully verified. Our results indicate that a detection limit of 14.7 nM of DNA was achieved, and non-specific DNAs with a single-base mismatch at the target DNA were selectively discriminated. This integrated detection system can be applied not only for single nucleotide polymorphism identification, but also for pathogen gene detection. The adoption of inexpensive paper and PDMS chips allows the fabrication of cost-effective detection systems. Moreover, it is very suitable for operation in various resource-limited locations by adopting a highly portable and user-friendly detection method that minimizes the use of large and expensive equipment.Supplementary information: The online version contains supplementary material available at 10.1007/s13206-023-00101-7.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":" ","pages":"1-11"},"PeriodicalIF":4.3,"publicationDate":"2023-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10134700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9720865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparison and Analysis of Polymer-Functionalized Carbon Nanotubes for Enhancement of the Quantitative Detection of Procalcitonin Levels in Human Plasma 高分子功能化碳纳米管增强人血浆降钙素原定量检测的比较与分析

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2023-04-27 DOI: 10.1007/s13206-023-00102-6

A. Schuck, Hyo Eun Kim, Minhee Kang, Yong-Sang Kim