Berliner und Munchener tierarztliche Wochenschrift最新文献_第8页

Potential role of real-time PCR for detecting Mycobacterium avium subsp. paratuberculosis in chronically diseased milking cows: a case control study. 实时荧光定量PCR检测鸟分枝杆菌亚种的潜在作用。慢性患病奶牛的副结核:一项病例对照研究。

IF 0.4 4区农林科学

Berliner und Munchener tierarztliche Wochenschrift Pub Date : 2016-07-01

Ahmad Alajmi, Günter Klein, Matthias Greiner, Nils Th Grabowski, Svenja Fohler, Amely Campe, Theresa Scheu, Martina Hoedemaker, Amir Abdulmawjood

{"title":"Potential role of real-time PCR for detecting Mycobacterium avium subsp. paratuberculosis in chronically diseased milking cows: a case control study.","authors":"Ahmad Alajmi, Günter Klein, Matthias Greiner, Nils Th Grabowski, Svenja Fohler, Amely Campe, Theresa Scheu, Martina Hoedemaker, Amir Abdulmawjood","doi":"","DOIUrl":"","url":null,"abstract":"Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP) is economically important to dairy operations. In the present study, a real-time PCR kit (certified for use in Germany) was used to detect MAP in bovine fecal and milk samples within a case control study examining different factors for their association with chronic disease in Northern Germany.The aim of this study was to describe the suitability of the MAP real-time PCR kit to detect MAP in feces and milk of chronically diseased dairy cows. For MAP detection a total of 928 fecal, 922 composite foremilk, and 92 bulk milk samples obtained from 58 cases and 35 control dairy herds were investigated. The real-time PCR showed MAP positive results for 11 (18.96%) and 6 (17.14%) of the case and control herds, respectively. All bulk milk samples were MAP negative. The results of fecal and milk samples were moderately correlated (kappa = 0.27). These data indicate that real-time PCR results have diagnostic value for diagnosing MAP positive animals, and that fecal samples are more suitable than milk samples for assessing the reasons for chronic disease on dairy farms.","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"129 7-8","pages":"304-9"},"PeriodicalIF":0.4,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34312023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seropositivity of Borrelia burgdorferi in a cohort of symptomatic cats from Europe based on a C6-peptide assay with discussion of implications in disease aetiology. 基于c6肽测定的一组欧洲有症状的猫的伯氏疏螺旋体血清阳性，并讨论了疾病病原学的意义。

IF 0.4 4区农林科学

Berliner und Munchener tierarztliche Wochenschrift Pub Date : 2016-07-01

Nikola Pantchev, Majda Globokar Vrhovec, Silvia Pluta, Reinhard K Straubinger

{"title":"Seropositivity of Borrelia burgdorferi in a cohort of symptomatic cats from Europe based on a C6-peptide assay with discussion of implications in disease aetiology.","authors":"Nikola Pantchev, Majda Globokar Vrhovec, Silvia Pluta, Reinhard K Straubinger","doi":"","DOIUrl":"","url":null,"abstract":"There are only few reports on Lyme borreliosis (LB) in cats. The reasons might be a different tick infestation in cats compared to dogs, a low susceptibility for tick-borne infections or a low awareness of veterinarians for tick-borne diseases in feline patients. The aim of this study was to determine the proportion of antibodies against Borrelia burgdorferi sensu lato (Bbsl) in feline sera, to compare the significance of feline versus canine LB, as well as to evaluate possible implications on disease occurrence. Specific antibodies against the C6-peptide of Bbsl in cats were detected by a rapid test based on enzyme immunoassay technique. The serum samples were sent to a diagnostic laboratory by veterinarians from Germany and other European countries with request for Borrelia serology in the years 2009-2011. Veterinarians were asked for information regarding the cats' location, age, gender, clinical signs, treatment and follow-up. In six of 271 (2.2%; 95% CI: 0.8-4.8%) cat sera, antibodies against the C6-peptide of Bbsl were detected. Proportion of Borrelia antibody-positive cat sera was significantly lower than the one determined for dogs during the same time period. All positive cats lived in countries endemic for LB (Germany, Sweden and Belgium), and all C6-antibody positive cats with the exception of one cat showed clinical signs. Possible implications on disease occurrence are discussed. Data presented here demonstrate a lower prevalence of Borrelia specific C6-antibodies in European cats when compared to dogs residing in the same regions. The absence of antibodies against Bbsl in 97.8% (95% CI: 95.2-99.2%) of the submitted samples indicate that diagnosis \"feline LB\"is rare in cats. Nevertheless, LB should be considered in cats with compatible clinical signs (e.g. shifting leg lameness, to less extent neurological signs) when other differential diagnoses are ruled out.","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"129 7-8","pages":"333-9"},"PeriodicalIF":0.4,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34312492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous occurrence of Salmonella enterica, Campylobacter spp. and Yersinia enterocolitica along the pork production chain from farm to meat processing in five conventional fattening pig herds in Lower Saxony. 在下萨克森州的五个传统育肥猪群中，从农场到肉类加工的猪肉生产链中同时发生肠沙门氏菌、弯曲杆菌和小肠结肠炎耶尔森菌。

IF 0.4 4区农林科学

Berliner und Munchener tierarztliche Wochenschrift Pub Date : 2016-07-01

Jana-Kristin Niemann, Thomas Alter, Greta Gölz, Erhard Tietze, Angelika Fruth, Wolfgang Rabsch, Christiane von Münchhausen, Roswitha Merle, Lothar Kreienbrock

{"title":"Simultaneous occurrence of Salmonella enterica, Campylobacter spp. and Yersinia enterocolitica along the pork production chain from farm to meat processing in five conventional fattening pig herds in Lower Saxony.","authors":"Jana-Kristin Niemann, Thomas Alter, Greta Gölz, Erhard Tietze, Angelika Fruth, Wolfgang Rabsch, Christiane von Münchhausen, Roswitha Merle, Lothar Kreienbrock","doi":"","DOIUrl":"","url":null,"abstract":"The objectives of this study were to gather data on the occurrence of Salmonella (S.) enterica, Campylobacter spp. and Yersinia (Y.) enterocolitica along the pork production chain and to further analyze detected Salmonella isolates by additionally applying MLVA (multiple-locus variable-number tandem repeat analysis). In total, 336 samples were collected at primary production, slaughter and meat processing from five conventional fattening pig farms and one common slaughterhouse. At farm level, S. enterica, Campylobacter spp. and Y. enterocolitica were detected in 19.4%, 38.9% and 11.1% of pooled fecal samples of fattening pigs. At slaughter, more than two-thirds of examined carcasses, 24% of carcass surfaces samples and about 60% of cecal content samples were positive for at least one of the examined pathogens. An amount of 4% of meat samples were positive for non-human pathogenic Y. enterocolitica. Identical MLVA patterns of Salmonella isolates from farm- and associated slaughterhouse samples demonstrated transmission across both production stages. Other MLVA patterns found at slaughter indicated possible colonization of pigs during transport or lairage and/or cross-contamination during slaughter. Identical MLVA patterns from risk tissues and the nearby carcass surface evidenced a direct contamination of carcasses as well. Overall, our data showed wide distribution ranges for all three examined pathogens within the pig production chain and underline the need for appropriate intervention strategies at pre- and postharvest.","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"129 7-8","pages":"296-303"},"PeriodicalIF":0.4,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34312022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The occurrence of "Candidatus Mycoplasma haemolamae" infections in clinically asymptomatic South American Camelids in Austria]. 【奥地利临床无症状南美骆驼中“血支原体候选菌”感染的发生】

IF 0.4 4区农林科学

Berliner und Munchener tierarztliche Wochenschrift Pub Date : 2016-07-01

Sonja Franz, Joachim Spergser, Ilse Schwendenwein, Anna Stanitznig, Bianca Lambacher, Alexander Tichy, Thomas Wittek

{"title":"[The occurrence of \"Candidatus Mycoplasma haemolamae\" infections in clinically asymptomatic South American Camelids in Austria].","authors":"Sonja Franz, Joachim Spergser, Ilse Schwendenwein, Anna Stanitznig, Bianca Lambacher, Alexander Tichy, Thomas Wittek","doi":"","DOIUrl":"","url":null,"abstract":"Reports of CMhl infections in South American Camelids in Europe are only available from the United Kingdom and Switzerland. Knowing that CMhl infections can lead to severe disease resulting in death if combined with other diseases or stress, it was the aim of this study to assess prevalence data from camelids in Austria. In comparison to the previous studies a representative number of camelids was investigated nationwide. Data were assessed due to differences in geographical region, age, sex, species, and origin. A relatively high prevalence of 25.8% was recorded. CMhl was detected significantly more often in alpacas (Vicunja pacos) than in llamas (Lama glama) and more frequently in animals younger than 2 years. Additionally regional differences have been observed, which might be due to climatic differences and/or variations in insect vectors. In this study apperantly clinical healthy animals were shown to be infected with CMhl. Camelids infected with CMhl are a pathogen reservoir. The results of this study indicate different risk levels of infection between llamas and alpacas and between younger and older animals. The data presented underline the necessity of further studies on CMhlI infections in South American Camelids.","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"129 7-8","pages":"318-22"},"PeriodicalIF":0.4,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34312025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From single nucleotide substitutions up to chromosomal deletions: genetic pause of leucism-associated disorders in animals. 从单核苷酸替换到染色体缺失:动物白血病相关疾病的遗传暂停。

IF 0.4 4区农林科学

Berliner und Munchener tierarztliche Wochenschrift Pub Date : 2016-07-01

Katharina Fleck, Georg Erhardt, Gesine Lühken

引用次数: 0

Interlaboratory Profiency Testing trial on the Detection of Staphylococcal Enterotoxins types SEA to SEE in food in Germany 2013. 2013年德国食品中葡萄球菌肠毒素类型SEA到SEE检测的实验室间熟练度测试试验。

IF 0.4 4区农林科学

Berliner und Munchener tierarztliche Wochenschrift Pub Date : 2016-07-01

Alexandra Fetsch, Katja Steege, Daniel Leeser, Gladys Krause

{"title":"Interlaboratory Profiency Testing trial on the Detection of Staphylococcal Enterotoxins types SEA to SEE in food in Germany 2013.","authors":"Alexandra Fetsch, Katja Steege, Daniel Leeser, Gladys Krause","doi":"","DOIUrl":"","url":null,"abstract":"In the autumn 2013, the National Reference Laboratory for coagulase positive staphylococci (CPS) including Staphylococcus (S.) aureus (NRL-Staph) at the Federal Institute for Risk Assessment has organized its first interlaboratory profiency testing (ILPT) trial for the detection of staphylococcal enterotoxins (SE) types SEA to SEE in food. The purpose of the ILPT was to assess the analytical competence of the official laboratories of the Federal German \"Länder\"authorities. Moreover, it was the intention to gain an overview of the standard methods implemented in the participating laboratories for the purpose of SE detection in food. Five cream cheese samples at three different contamination levels (blank, low, and high) were sent to each participant. In total, 15 laboratories participated to the ILPT: 14 laboratories from 11 Federal German \"Länder\", and the European Reference Laboratory for CPS including S. aureus (EU-RL for CPS). Data sets from 14 participating laboratories were included in the analysis. Overall, a specificity of 100% (14/14 true negative results), a sensitivity of 55% (31/56 true positive results), and an accuracy of 64% (45/60 true results) was achieved. The majority of participants (9/15) used other analytical methods for the detection of SE in food than the suggested European Screening Method (ESM) v5. To conclude on the ILPT in general it is to state that the majority of participating laboratories failed to correctly identify SE-low-contaminated samples. Further efforts are necessary to improve the analytical capacity and sensitivity as regards the detection of SE in food in Germany.","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"129 7-8","pages":"290-5"},"PeriodicalIF":0.4,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34312021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ruminal Leiomyosarcoma in an adult cow. 成年牛瘤胃平滑肌肉瘤。

IF 0.4 4区农林科学

Berliner und Munchener tierarztliche Wochenschrift Pub Date : 2016-07-01 DOI: 10.2376/0005-9366-15100

J. Benavides, M. Fuertes, V. Pérez, L. Delgado, M. C. Ferreras

引用次数: 3

Evaluation of CO2 anaesthesia applied by a commercial device for the castration of male suckling piglets under field conditions. 在野外条件下，商业装置用于雄性哺乳仔猪阉割的CO2麻醉的评价。

IF 0.4 4区农林科学

Berliner und Munchener tierarztliche Wochenschrift Pub Date : 2016-07-01

Rebecca Langhoff, Ulrike Auer, Julia Maneng, Agnes Hochgerner, Mathias Ritzmann

{"title":"Evaluation of CO2 anaesthesia applied by a commercial device for the castration of male suckling piglets under field conditions.","authors":"Rebecca Langhoff, Ulrike Auer, Julia Maneng, Agnes Hochgerner, Mathias Ritzmann","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this study was to describe clinical and behavioural parameters of piglets castrated with the use of CO2 anaesthesia by using the commercial available device MS Pigsleeper. 80 male piglets with three to six days of age were allocated to four groups, and were castrated or left uncastrated either after receiving 70% CO2/30% O02, or without anaesthesia. Defensive movements and heart rates were measured during induction and castration phase. At castration phase the dewclaw reflex was tested and piglets were monitored for vocalisation. During recovery and the subsequent five minutes conspicuous behaviours were recorded. Intensity of defensive movements was significantly higher in groups with CO2 anaesthesia during induction phase. Defensive movements were significantly lower in groups with CO2, but also not fully absent, during castration phase. Surgical tolerance was only reached by 20% (4/20) of piglets castrated under anaesthesia. Heart rates were above physiological levels at the beginning of induction phase and dropped significantly thereafter. Dropping of heart rates was more pronounced in anaesthetised groups leading to significant differences compared to non-anaesthetised groups. A minimum heart rate of 20 beats per minute was measured. Conspicuous behaviours as spasms, gasping and licking were described for pigs after CO2 anaesthesia. Measured parameters implicated stress and discomfort during induction and recovery phase and severe cardiovascular depression during CO2 anaesthesia. Surgical tolerance was reached by only a small proportion of anaesthetised piglets. Therefore, welfare aspects were not met using CO2 anaesthesia for piglet castration in general and especially by applying the gas anaesthesia with the commercial device MS Pigsleeper.","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"129 7-8","pages":"282-9"},"PeriodicalIF":0.4,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34312020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ruminal Leiomyosarcoma in an adult cow. 成年牛瘤胃平滑肌肉瘤。

IF 0.4 4区农林科学

Berliner und Munchener tierarztliche Wochenschrift Pub Date : 2016-07-01

Julio Benavides, Miguel Fuertes, Valentin Pérez, Laetitia Delgado, Ma Carmen Ferreras

引用次数: 0

Potential role of real-time PCR for detecting Mycobacterium avium subsp. paratuberculosis in chronically diseased milking cows: a case control study. 实时荧光定量PCR检测鸟分枝杆菌亚种的潜在作用。慢性患病奶牛的副结核:一项病例对照研究。

IF 0.4 4区农林科学

Berliner und Munchener tierarztliche Wochenschrift Pub Date : 2016-07-01 DOI: 10.2376/0005-9366-15103

A. Alajmi, G. Klein, M. Greiner, N. Grabowski, Svenja Fohler, A. Campe, T. Scheu, M. Hoedemaker, A. Abdulmawjood

{"title":"Potential role of real-time PCR for detecting Mycobacterium avium subsp. paratuberculosis in chronically diseased milking cows: a case control study.","authors":"A. Alajmi, G. Klein, M. Greiner, N. Grabowski, Svenja Fohler, A. Campe, T. Scheu, M. Hoedemaker, A. Abdulmawjood","doi":"10.2376/0005-9366-15103","DOIUrl":"https://doi.org/10.2376/0005-9366-15103","url":null,"abstract":"Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP) is economically important to dairy operations. In the present study, a real-time PCR kit (certified for use in Germany) was used to detect MAP in bovine fecal and milk samples within a case control study examining different factors for their association with chronic disease in Northern Germany.The aim of this study was to describe the suitability of the MAP real-time PCR kit to detect MAP in feces and milk of chronically diseased dairy cows. For MAP detection a total of 928 fecal, 922 composite foremilk, and 92 bulk milk samples obtained from 58 cases and 35 control dairy herds were investigated. The real-time PCR showed MAP positive results for 11 (18.96%) and 6 (17.14%) of the case and control herds, respectively. All bulk milk samples were MAP negative. The results of fecal and milk samples were moderately correlated (kappa = 0.27). These data indicate that real-time PCR results have diagnostic value for diagnosing MAP positive animals, and that fecal samples are more suitable than milk samples for assessing the reasons for chronic disease on dairy farms.","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"98 1","pages":"304-9"},"PeriodicalIF":0.4,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73438927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1