Biochemical medicine and metabolic biology最新文献_第3页

13C NMR-Study of C2- and C3-Deuterated Lactic Acid Production by Parotid Cells Exposed to 13C-Labeled Glucose in the Presence of D2O 13C核磁共振成像研究在D2O存在下，暴露于13C标记葡萄糖的腮腺细胞产生C2和c3氘乳酸

Biochemical medicine and metabolic biology Pub Date : 1994-10-01 DOI: 10.1006/bmmb.1994.1059

Malaisse W.J., Biesemans M., Sener A., Willem R.

引用次数: 5

Hexokinase Autophosphorylation: Identification of a New Dual Specificity Protein Kinase 己糖激酶自磷酸化:一种新的双特异性蛋白激酶的鉴定

Biochemical medicine and metabolic biology Pub Date : 1994-10-01 DOI: 10.1006/bmmb.1994.1061

Adams V., Schieber A., Mccabe E.R.B.

引用次数: 6

Mannose-Coated Liposomal Hamycin in the Treatment of Experimental Leishmaniasis in Hamsters 甘露糖包被脂质体哈霉素治疗实验性仓鼠利什曼病

Biochemical medicine and metabolic biology Pub Date : 1994-10-01 DOI: 10.1006/bmmb.1994.1050

Banerjee G., Bhaduri A.N., Basu M.K.

引用次数: 28

Platelet-Derived Growth Factor (PDGF) Rapidly Stimulates Binding of Glycolytic Enzymes to Muscle Cytoskeleton, Prevented by Calmodulin Antagonists 血小板衍生生长因子(PDGF)快速刺激糖酵解酶与肌肉细胞骨架的结合，被钙调素拮抗剂阻止

Biochemical medicine and metabolic biology Pub Date : 1994-10-01 DOI: 10.1006/bmmb.1994.1054

Livnat T., Chenzion M., Beitner R.

{"title":"Platelet-Derived Growth Factor (PDGF) Rapidly Stimulates Binding of Glycolytic Enzymes to Muscle Cytoskeleton, Prevented by Calmodulin Antagonists","authors":"Livnat T., Chenzion M., Beitner R.","doi":"10.1006/bmmb.1994.1054","DOIUrl":"10.1006/bmmb.1994.1054","url":null,"abstract":"<div><p>Glycolytic enzymes are known to be controlled by reversible binding to cytoskeleton. Our previous experiments have shown that insulin, epidermal growth factor (EGF), and Ca<sup>2+</sup> induce a rapid and transient stimulation of binding of glycolytic enzymes to muscle cytoskeleton. We show here that platelet-derived growth factor (PDGF) exerts a similar action. Incubation of rat diaphragm muscle in the presence of PDGF resulted in rapid and transient stimulation of binding of phosphofructokinase (EC 2.7.1.11) and aldolase (EC 4.1.2.13) to muscle cytoskeleton. The increase in cytoskeleton-bound glycolytic enzymes induced by PDGF was prevented by treatment with the calmodulin antagonists trifluoperazine or CGS 9343B (a potent and selective inhibitor of calmodulin activity), which strongly suggests that Ca<sup>2+</sup>-calmodulin is involved in this effect of PDGF. Similarly, we previously found that stimulation of cytoskeleton-bound glycolytic enzymes exerted by insulin, EGF, or Ca<sup>2+</sup>, was also calmodulin mediated. The present and previous results suggest that the rapid, Ca<sup>2+</sup>-calmodulin-mediated increase in cytoskeleton-bound glycolytic enzymes, may be a general mechanism in the cell, in signal transduction of insulin, growth factors, and other Ca<sup>2+</sup>-mobilizing hormones. The accelerated cytoskeletal glycolysis will provide local ATP, which is required for the rapid cytoskeletal-membrane rearrangements following binding of growth factor or hormone to its receptor.</p></div>","PeriodicalId":8752,"journal":{"name":"Biochemical medicine and metabolic biology","volume":"53 1","pages":"Pages 28-33"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/bmmb.1994.1054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18855482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Nonisotopic Identification of Two Point Mutations in the CYP21 Gene Responsible for Nonclassic 21-Hydroxylase Deficiency 导致非经典21-羟化酶缺乏症的CYP21基因两点突变的非同位素鉴定

Biochemical medicine and metabolic biology Pub Date : 1994-08-01 DOI: 10.1006/bmmb.1994.1037

Shevtsov S.P., Rechitsky S., Verlinsky O., Schwartz E.I.

引用次数: 0

The Methylfolate Axis in Neural Tube Defects: In Vitro Characterization and Clinical Investigation 甲基叶酸轴在神经管缺损中的作用:体外表征和临床研究

Biochemical medicine and metabolic biology Pub Date : 1994-08-01 DOI: 10.1006/bmmb.1994.1040

Lucock M.D., Wild J., Schorah C.J., Levene M.I., Hartley R.

{"title":"The Methylfolate Axis in Neural Tube Defects: In Vitro Characterization and Clinical Investigation","authors":"Lucock M.D., Wild J., Schorah C.J., Levene M.I., Hartley R.","doi":"10.1006/bmmb.1994.1040","DOIUrl":"10.1006/bmmb.1994.1040","url":null,"abstract":"<div><p>We have investigated various micronutrients important to folate metabolism in women with two previous neural tube defect (NTD)-affected pregnancies. Results suggest the disposition of plasma 5-methyltetrahydrofolate (5CH<sub>3</sub>-H<sub>4</sub>PteGlu) with respect to dietary intake may differ from that of the control population. It appears that to achieve a given plasma level of 5CH<sub>3</sub>-H<sub>4</sub>PteGlu, the population with a history of NTD pregnancies needs to take in more dietary folate than controls. We discuss this in the context of a potential lesion at or upstream from 5,10-methylenetetrahydrofolate reductase (MTHFR). This metabolic axis, which is responsible for the multienzymic conversion of PteGlu to 5CH<sub>3</sub>-H<sub>4</sub>PteGlu, has been investigated in a rat model using liver homogenate. The anticonvulsant drug (ACD) carbamazepine was found to inhibit the reaction in terms of a reduced <em>V</em><sub>max</sub> and increased <em>K<sub>m</sub></em>. Inhibition approaching maximal was found to occur at therapeutic levels of ACD. Various potential inhibitory sites along the methylfolate axis are considered and possible relationships to congenital malformations discussed. We describe folate and one carbon metabolism in relation to potential NTD lesion sites, not only in the light of present findings, but with respect to the published findings of other workers. Based on our hypothesis that an NTD lesion exists upstream from MTHFR, we expound how pteroylmonoglutamate supplementation may protect against NTD (i) by reducing endotoxic homocysteine and (ii) through inhibiting MTHFR (as do dihydrofolates) and thus diverting one carbon units into DNA thymine.</p></div>","PeriodicalId":8752,"journal":{"name":"Biochemical medicine and metabolic biology","volume":"52 2","pages":"Pages 101-114"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/bmmb.1994.1040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18990656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Erythropoietin (EPO) Levels in Fetal Rats after Ritodrine and Terbutaline Administration 利托普利和特布他林给药后胎鼠红细胞生成素(EPO)水平变化

Biochemical medicine and metabolic biology Pub Date : 1994-08-01 DOI: 10.1006/bmmb.1994.1043

Ibrahim H., Kahn E., Harper R.G., Wapnir R.A.

{"title":"Erythropoietin (EPO) Levels in Fetal Rats after Ritodrine and Terbutaline Administration","authors":"Ibrahim H., Kahn E., Harper R.G., Wapnir R.A.","doi":"10.1006/bmmb.1994.1043","DOIUrl":"10.1006/bmmb.1994.1043","url":null,"abstract":"<div><p>Beta-2 sympathomimetics, such as terbutaline, hare been shown to alter erythropoietin (EPO) secretion in animal studies. More recently introduced sympathomimetics, such as ritodrine, have been extensively used to inhibit uterine contractions in preterm labor. It has not been determined what effect ritodrine may have on EPO production. We investigated the effect of RD administered to rats in the last day of gestation on the dam and the fetuses′ levels of EPO. Rats at the 20th day of gestation were given, under anesthesia, either 3000 μg/kg ritodrine over a 10-min period or a similar volume of saline as control (CTL). Fetuses were removed at 0 and 4 h after injection. Ritodrine administration produced a decrease of dam EPO (23.4 ± 4.2 to 12.0 ± 2.9 pmol/ml), while the CTL showed no changes. The fetuses from the ritodrine-injected dams exhibited a marked decline over the 4-h period, while the CTL fetuses did not change. In other experiments 500 μg terbutaline was administered daily during the last 5 days of rat gestation. The drug produced a significant decline in EPO at delivery time, but plasma EPO in the pups was unchanged. Blood hematocrits were comparable to those of controls. The data show that acute administration of ritodrine reduces fetal and dam plasma EPO, while long-term terbutaline treatment late in gestation alters maternal, but not fetal plasma EPO, indicating that neither drug has any direct regulatory effect on the erythropoietic ability of the fetuses.</p></div>","PeriodicalId":8752,"journal":{"name":"Biochemical medicine and metabolic biology","volume":"52 2","pages":"Pages 128-131"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/bmmb.1994.1043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18990659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A Caucasian Family with the 3271 Mutation in Mitochondrial DNA 线粒体DNA 3271突变的高加索家族

Biochemical medicine and metabolic biology Pub Date : 1994-08-01 DOI: 10.1006/bmmb.1994.1045

Marie S.K.N., Goto Y., Passosbueno M.R., Zatz M., Carvalho A.A.S., Carvalho M., Levy J.A., Palou V.B., Campiotto S., Horai S., Nonaka I.

引用次数: 24

Inhibitory Effect of Fatty Acids on Glucose and Insulin Uptake in the Perfused Rat Hindquarter 脂肪酸对灌注大鼠后躯葡萄糖和胰岛素摄取的抑制作用

Biochemical medicine and metabolic biology Pub Date : 1994-08-01 DOI: 10.1006/bmmb.1994.1039

Ikeda T., Terasawa H., Ishimura M., Ochi H., Noguchi I., Fujiyama K., Hoshino T., Tanaka Y., Mashiba H.

{"title":"Inhibitory Effect of Fatty Acids on Glucose and Insulin Uptake in the Perfused Rat Hindquarter","authors":"Ikeda T., Terasawa H., Ishimura M., Ochi H., Noguchi I., Fujiyama K., Hoshino T., Tanaka Y., Mashiba H.","doi":"10.1006/bmmb.1994.1039","DOIUrl":"10.1006/bmmb.1994.1039","url":null,"abstract":"<div><p>To elucidate the effects of fatty acids on the uptake of glucose and insulin in the muscle, the effects of oleic and palmitic acids on the uptake of glucose and insulin were investigated in perfused hindquarters of rats. In the absence of insulin, glucose uptake in the hindquarter was slightly, but not significantly, decreased by the addition of oleic and palmitic acids. In the presence of 100 or 500 μU/ml insulin, glucose uptake in the hindquarter (243 ± 58 or 282 ± 65 μmol/30 min) was significantly decreased by the addition of 1000 μM oleic acid and 500 μM palmitic acid (175 ± 50 or 207 ± 47 μmol/30 min). The decrease in insulin uptake rate, although not significant at 500 μU/ml insulin, is of similar magnitude to the decrease in glucose uptake. In the presence of 1000 μU/ml insulin, glucose and insulin uptake was not significantly altered by the addition of fatty acids. These results indicate that fatty acids directly inhibit the muscular glucose uptake via the decrease in muscular insulin uptake at a physiological concentration of insulin.</p></div>","PeriodicalId":8752,"journal":{"name":"Biochemical medicine and metabolic biology","volume":"52 2","pages":"Pages 97-100"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/bmmb.1994.1039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18988664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Membrane Fluidity Is Different in Intact Erythrocytes and Ghost Membranes 完整红细胞和鬼膜的膜流动性不同

Biochemical medicine and metabolic biology Pub Date : 1994-08-01 DOI: 10.1006/bmmb.1994.1044

Tong P., Thomas T., Wilkinson R.

引用次数: 6