Nuclear receptor signaling最新文献_第6页

Selective androgen receptor modulators in preclinical and clinical development. 临床前和临床开发中的选择性雄激素受体调节剂。

Nuclear receptor signaling Pub Date : 2008-01-01 Epub Date: 2008-11-26 DOI: 10.1621/nrs.06010

Ramesh Narayanan, Michael L Mohler, Casey E Bohl, Duane D Miller, James T Dalton

引用次数: 133

MTA family of coregulators in nuclear receptor biology and pathology. 核受体生物学和病理学中的MTA家族共调节因子。

Nuclear receptor signaling Pub Date : 2007-11-30 DOI: 10.1621/nrs.05010

Bramanandam Manavathi, Kamini Singh, Rakesh Kumar

引用次数: 97

The coregulator Alien. 共同调节器外星人。

Nuclear receptor signaling Pub Date : 2007-11-30 DOI: 10.1621/nrs.05008

Maria Papaioannou, Christian Melle, Aria Baniahmad

引用次数: 13

Steroid receptor coactivator 2 is required for female fertility and mammary morphogenesis: insights from the mouse, relevance to the human. 类固醇受体辅激活因子2是女性生育和乳腺形态发生所必需的:来自小鼠的见解，与人类的相关性。

Nuclear receptor signaling Pub Date : 2007-11-30 DOI: 10.1621/nrs.05011

Atish Mukherjee, Paula Amato, D Craig Allred, Francesco J DeMayo, John P Lydon

{"title":"Steroid receptor coactivator 2 is required for female fertility and mammary morphogenesis: insights from the mouse, relevance to the human.","authors":"Atish Mukherjee, Paula Amato, D Craig Allred, Francesco J DeMayo, John P Lydon","doi":"10.1621/nrs.05011","DOIUrl":"https://doi.org/10.1621/nrs.05011","url":null,"abstract":"Although the importance of the progesterone receptor (PR) to female reproductive and mammary gland biology is firmly established, the coregulators selectively co-opted by PR in these systems have not been clearly delineated. A selective gene-knockout approach applied to the mouse, which abrogates gene function only in cell types that express PR, recently disclosed steroid receptor coactivator 2 (SRC-2, also known as TIF-2 or GRIP-1) to be an indispensable coregulator for uterine and mammary gland responses that require progesterone. Uterine cells positive for PR (but devoid of SRC-2) were found to be incapable of facilitating embryo implantation, a necessary first step toward the establishment of the materno-fetal interface. Importantly, such an implantation defect is not exhibited by knockouts for SRC-1 or SRC-3, underscoring the unique coregulator importance of SRC-2 in peri-implantation biology. Moreover, despite normal levels of PR, SRC-1 and SRC-3, progesterone-dependent branching morphogenesis and alveologenesis fails to occur in the murine mammary gland in the absence of SRC-2, thereby establishing a critical coregulator role for SRC-2 in signaling cascades that mediate progesterone-induced mammary epithelial proliferation. Finally, the recent detection of SRC-2 in the human endometrium and breast suggests that this coregulator may represent a new clinical target for the future management of female reproductive health and/or breast cancer.","PeriodicalId":87415,"journal":{"name":"Nuclear receptor signaling","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1621/nrs.05011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27205906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40

The NR3B subgroup: an ovERRview. NR3B亚组综述。

Nuclear receptor signaling Pub Date : 2007-11-30 DOI: 10.1621/nrs.05009

Annie M Tremblay, Vincent Giguère

引用次数: 123

The mineralocorticoid receptor: insights into its molecular and (patho)physiological biology. 矿物皮质激素受体:对其分子和(病理)生理生物学的见解。

Nuclear receptor signaling Pub Date : 2007-11-30 DOI: 10.1621/nrs.05012

Say Viengchareun, Damien Le Menuet, Laetitia Martinerie, Mathilde Munier, Laurent Pascual-Le Tallec, Marc Lombès

{"title":"The mineralocorticoid receptor: insights into its molecular and (patho)physiological biology.","authors":"Say Viengchareun, Damien Le Menuet, Laetitia Martinerie, Mathilde Munier, Laurent Pascual-Le Tallec, Marc Lombès","doi":"10.1621/nrs.05012","DOIUrl":"https://doi.org/10.1621/nrs.05012","url":null,"abstract":"The last decade has witnessed tremendous progress in the understanding of the mineralocorticoid receptor (MR), its molecular mechanism of action, and its implications for physiology and pathophysiology. After the initial cloning of MR, and identification of its gene structure and promoters, it now appears as a major actor in protein-protein interaction networks. The role of transcriptional coregulators and the determinants of mineralocorticoid selectivity have been elucidated. Targeted oncogenesis and transgenic mouse models have identified unexpected sites of MR expression and novel roles for MR in non-epithelial tissues. These experimental approaches have contributed to the generation of new cell lines for the characterization of aldosterone signaling pathways, and have also facilitated a better understanding of MR physiology in the heart, vasculature, brain and adipose tissues. This review describes the structure, molecular mechanism of action and transcriptional regulation mediated by MR, emphasizing the most recent developments at the cellular and molecular level. Finally, through insights obtained from mouse models and human disease, its role in physiology and pathophysiology will be reviewed. Future investigations of MR biology should lead to new therapeutic strategies, modulating cell-specific actions in the management of cardiovascular disease, neuroprotection, mineralocorticoid resistance, and metabolic disorders.","PeriodicalId":87415,"journal":{"name":"Nuclear receptor signaling","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1621/nrs.05012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27205909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 236

Steroid receptor RNA activator (SRA1): unusual bifaceted gene products with suspected relevance to breast cancer. 类固醇受体RNA激活因子(SRA1):怀疑与乳腺癌相关的不寻常的双面基因产物。

Nuclear receptor signaling Pub Date : 2007-08-03 DOI: 10.1621/nrs.05006

Etienne Leygue

引用次数: 123

Evaluation of steroid hormone receptor protein expression in intact cells using flow cytometry. 流式细胞术评价完整细胞中类固醇激素受体蛋白的表达。

Nuclear receptor signaling Pub Date : 2007-08-03 DOI: 10.1621/nrs.05007

Cherie L Butts, Shetha A Shukair, Kristina M Duncan, Christopher W Harris, Elena Belyavskaya, Esther M Sternberg

{"title":"Evaluation of steroid hormone receptor protein expression in intact cells using flow cytometry.","authors":"Cherie L Butts, Shetha A Shukair, Kristina M Duncan, Christopher W Harris, Elena Belyavskaya, Esther M Sternberg","doi":"10.1621/nrs.05007","DOIUrl":"https://doi.org/10.1621/nrs.05007","url":null,"abstract":"Several methods are currently employed to evaluate expression of steroid hormone receptors in tissues and cells, including real-time reverse-transcriptase polymerase chain reaction (real-time RT-PCR) and western blot assays. These methods require homogenization of cells, thereby preventing evaluation of individual cells or specific cell types in a given tissue sample. In addition, methods such as real-time RT-PCR assess mRNA levels, which may be subject to posttranslational modifications that prevent subsequent production of functional proteins. Flow cytometry is a fluorescence-based technique commonly used to evaluate expression of cell surface and intracellular proteins. This method is especially useful as it allows for single-cell analysis and can be utilized to determine the amount of receptor expressed by individual cells. Flow cytometry is commonly used to analyze immune cell activity and determine functionality based on changes in expression of cell surface molecules, as well as intracellular proteins (such as cytokines). Here, we describe a method to identify protein expression of steroid hormone receptors by rat leukocytes from different organs (spleen, liver and thymus) using flow cytometry. We examined expression of glucocorticoid receptor (GR), androgen receptor (AR) and progesterone receptor (PR) by cells at these sites and were able to demonstrate expression of receptors, as well as the intensity of expression of each receptor. This method is useful for rapid, high throughput measurement of steroid hormone receptors at the protein level in single, intact cells and would be valuable to determine which cells are more likely to respond to steroid hormone treatment.","PeriodicalId":87415,"journal":{"name":"Nuclear receptor signaling","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1621/nrs.05007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26899972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Kinases and protein phosphorylation as regulators of steroid hormone action. 激酶和蛋白磷酸化作为类固醇激素作用的调节因子。

Nuclear receptor signaling Pub Date : 2007-05-17 DOI: 10.1621/nrs.05005

Nancy L Weigel, Nicole L Moore

{"title":"Kinases and protein phosphorylation as regulators of steroid hormone action.","authors":"Nancy L Weigel, Nicole L Moore","doi":"10.1621/nrs.05005","DOIUrl":"https://doi.org/10.1621/nrs.05005","url":null,"abstract":"Although the primary signal for the activation of steroid hormone receptors is binding of hormone, there is increasing evidence that the activities of cell signaling pathways and the phosphorylation status of these transcription factors and their coregulators determine the overall response to the hormone. In some cases, enhanced cell signaling is sufficient to cause activation of receptors in medium depleted of steroids. Steroid receptors are targets for multiple kinases. Many of the phosphorylation sites contain Ser/Thr-Pro motifs implicating proline-directed kinases such as the cyclin-dependent kinases and the mitogen-activated kinases (MAPK) in receptor phosphorylation. Although some sites are constitutively phosphorylated, others are phosphorylated in response to hormone. Still others are only phosphorylated in response to specific cell signaling pathways. Phosphorylation of specific sites has been implicated not only in overall transcriptional activity, but also in nuclear localization, protein stability, and DNA binding. The studies of the roles of phosphorylation in coregulator function are more limited, but it is now well established that many of them are highly phosphorylated and that phosphorylation regulates their function. There is good evidence that some of the phosphorylation sites in the receptors and coregulators are targets of multiple signaling pathways. Individual sites have been associated both with functions that enhance the activity of the receptor, as well as with functions that inhibit activity. Thus, the specific combinations of phosphorylations of the steroid receptor combined with the expression levels and phosphorylation status of coregulators will determine the genes regulated and the biological response.","PeriodicalId":87415,"journal":{"name":"Nuclear receptor signaling","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1621/nrs.05005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26743107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 66

Functional and biological properties of the nuclear receptor coregulator PELP1/MNAR. 核受体共调节因子PELP1/MNAR的功能和生物学特性。

Nuclear receptor signaling Pub Date : 2007-05-17 DOI: 10.1621/nrs.05004

Ratna K Vadlamudi, Rakesh Kumar

引用次数: 86