Mechanics & chemistry of biosystems : MCB最新文献_第2页

Ablation of cytoskeletal filaments and mitochondria in live cells using a femtosecond laser nanoscissor. 用飞秒激光纳米剪刀消融活细胞中的细胞骨架细丝和线粒体。

Mechanics & chemistry of biosystems : MCB Pub Date : 2005-01-01 DOI: 10.3970/MCB.2005.002.017

N. Shen, D. Datta, C. Schaffer, P. Leduc, D. Ingber, E. Mazur

引用次数: 143

Shear force at the cell-matrix interface: enhanced analysis for microfabricated post array detectors. 细胞-基质界面的剪切力:微制造柱阵列探测器的增强分析。

Mechanics & chemistry of biosystems : MCB Pub Date : 2005-01-01

Christopher A Lemmon, Nathan J Sniadecki, Sami Alom Ruiz, John L Tan, Lewis H Romer, Christopher S Chen

{"title":"Shear force at the cell-matrix interface: enhanced analysis for microfabricated post array detectors.","authors":"Christopher A Lemmon, Nathan J Sniadecki, Sami Alom Ruiz, John L Tan, Lewis H Romer, Christopher S Chen","doi":"","DOIUrl":"","url":null,"abstract":"The interplay of mechanical forces between the extracellular environment and the cytoskeleton drives development, repair, and senescence in many tissues. Quantitative definition of these forces is a vital step in understanding cellular mechanosensing. Microfabricated post array detectors (mPADs) provide direct measurements of cell-generated forces during cell adhesion to extracellular matrix. A new approach to mPAD post labeling, volumetric imaging, and an analysis of post bending mechanics determined that cells apply shear forces and not point moments at the matrix interface. In addition, these forces could be accurately resolved from post deflections by using images of post tops and bases. Image analysis tools were then developed to increase the precision and throughput of post centroid location. These studies resulted in an improved method of force measurement with broad applicability and concise execution using a fully automated force analysis system. The new method measures cell-generated forces with less than 5% error and less than 90 seconds of computational time. Using this approach, we demonstrated direct and distinct relationships between cellular traction force and spread cell surface area for fibroblasts, endothelial cells, epithelial cells and smooth muscle cells.","PeriodicalId":87411,"journal":{"name":"Mechanics & chemistry of biosystems : MCB","volume":"2 1","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1480360/pdf/nihms-5985.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26032556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth, anisotropy, and residual stresses in arteries. 动脉的生长、各向异性和残余应力。

Mechanics & chemistry of biosystems : MCB Pub Date : 2005-01-01 DOI: 10.3970/MCB.2005.002.027

K. Volokh, Y. Lev

引用次数: 11

Ablation of cytoskeletal filaments and mitochondria in live cells using a femtosecond laser nanoscissor. 用飞秒激光纳米剪刀消融活细胞中的细胞骨架细丝和线粒体。

Mechanics & chemistry of biosystems : MCB Pub Date : 2005-01-01

Nan Shen, Dabajyoti Datta, Chris B Schaffer, Philip LeDuc, Donald E Ingber, Eric Mazur

引用次数: 0

The effect of longitudinal pre-stretch and radial constraint on the stress distribution in the vessel wall: a new hypothesis. 纵向预拉伸和径向约束对血管壁应力分布的影响:一个新的假设。

Mechanics & chemistry of biosystems : MCB Pub Date : 2005-01-01 DOI: 10.3970/MCB.2005.002.041

Wei Zhang, Carly Herrera, S. Atluri, G. Kassab

{"title":"The effect of longitudinal pre-stretch and radial constraint on the stress distribution in the vessel wall: a new hypothesis.","authors":"Wei Zhang, Carly Herrera, S. Atluri, G. Kassab","doi":"10.3970/MCB.2005.002.041","DOIUrl":"https://doi.org/10.3970/MCB.2005.002.041","url":null,"abstract":"It is well known that blood vessels shorten axially when excised. This is due to the perivascular tethering constraint by side branches and the existence of pre-stretch of blood vessels at the in situ state. Furthermore, vessels are radially constrained to various extents by the surrounding tissues at physiological loading. Our hypothesis is that the axial pre-stretch and radial constraint by the surrounding tissue homogenizes the stress and strain distributions in the vessel wall. A finite element analysis of porcine coronary artery and rabbit thoracic aorta based on measured material properties, geometry, residual strain and physiological loading is used to compute the intramural stresses and strains. We systematically examined the effect of pre-stretch and external radial constraint in both vessels. Our results show that both stretching in the axial direction and compression in the radial direction lead to a more homogeneous strain and stress state in the blood vessel wall. A \"uniform biaxial strain\" hypothesis is proposed for the blood vessel wall and the ramifications are discussed.","PeriodicalId":87411,"journal":{"name":"Mechanics & chemistry of biosystems : MCB","volume":"2 1 1","pages":"41-52"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70240440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

The effect of longitudinal pre-stretch and radial constraint on the stress distribution in the vessel wall: a new hypothesis. 纵向预拉伸和径向约束对血管壁应力分布的影响:一个新的假设。

Mechanics & chemistry of biosystems : MCB Pub Date : 2005-01-01

Wei Zhang, Carly Herrera, Satya N Atluri, Ghassan S Kassab

引用次数: 0

Endothelial cells as mechanical transducers: enzymatic activity and network formation under cyclic strain. 内皮细胞作为机械换能器:循环应变下的酶活性和网络形成。

Mechanics & chemistry of biosystems : MCB Pub Date : 2004-12-01 DOI: 10.3970/MCB.2004.001.279

A. Shukla, A. R. Dunn, M. Moses, K. Vliet

{"title":"Endothelial cells as mechanical transducers: enzymatic activity and network formation under cyclic strain.","authors":"A. Shukla, A. R. Dunn, M. Moses, K. Vliet","doi":"10.3970/MCB.2004.001.279","DOIUrl":"https://doi.org/10.3970/MCB.2004.001.279","url":null,"abstract":"Although it is established that endothelial cells can respond to external mechanical cues (e.g., alignment in the direction of fluid shear stress), the extent to which mechanical stress and strain applied via the endothelial cell substrate impact biomolecular and cellular processes is not well-understood. This issue is particularly important in the context of inflammation, vascular remodeling, and cancer progression, as each of these processes occurs concurrently with localized increases in strain and marked changes in molecules secreted by adjacent cells. Here, we systematically vary the level and duration of cyclic tensile strain applied to human dermal microvascular and bovine capillary endothelial cells via substrate deflection, and then correlate these cues with the secretion of extracellular matrix-degrading enzymes and a morphological transition from confluent monolayers to well-defined multicellular networks that resemble capillary tube-like structures. For a constant chemical environment, we find that super-physiological mechanical strain stimulates both endothelial cell secretion of latent matrix metalloprotease-2 and multicellular networks in a time- and strain-dependent manner. These results demonstrate coupling between the mechanical and biochemical states of microvascular endothelial cells, and indicate that elevated local stress may directly impact new capillary growth (angiogenesis) toward growing tumors and at capillary wall defect sites.","PeriodicalId":87411,"journal":{"name":"Mechanics & chemistry of biosystems : MCB","volume":"1 4 1","pages":"279-90"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70240505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Forces required to initiate membrane tether extrusion from cell surface depend on cell type but not on the surface molecule. 从细胞表面启动膜系索挤压所需的力取决于细胞类型，而不取决于表面分子。

Mechanics & chemistry of biosystems : MCB Pub Date : 2004-12-01 DOI: 10.3970/MCB.2004.001.245

W. Marcus, R. McEver, C. Zhu

{"title":"Forces required to initiate membrane tether extrusion from cell surface depend on cell type but not on the surface molecule.","authors":"W. Marcus, R. McEver, C. Zhu","doi":"10.3970/MCB.2004.001.245","DOIUrl":"https://doi.org/10.3970/MCB.2004.001.245","url":null,"abstract":"When a cell adhered to another cell or substratum via surface proteins is forced to detach, lipid membrane tethers are often extruded from the cell surface before the protein bond dissociates. For example, during the inflammatory reaction leukocytes roll on the surface of activated endothelial cells. The rolling adhesion is mediated by interactions of selectins with their ligands, e.g., P-selectin glycoprotein ligand (PSGL)-1, which extrudes membrane tethers from the surfaces of both leukocytes and endothelial cells. Membrane tether extrusion has been suggested to regulate leukocyte rolling. Here we examine several factors that may affect forces required to initiate membrane tethers, or initial tether force. It was found that initial tether forces were similar regardless of the presence or absence of the cytoplasmic tail of P-selectin and regardless of whether the tethers were extruded via binding to PSGL-1 or Fcy receptors. Initial tether forces were found to depend on the cell types tested and were greatly reduced by treatment of latrunculin A, which inhibits actin polymerization. These data provide additional insights to the control of membrane tether extrusion, which should be taken into account when cellular functions such as rolling where tether extrusion plays a regulatory role are compared using different cell types expressing the same molecule.","PeriodicalId":87411,"journal":{"name":"Mechanics & chemistry of biosystems : MCB","volume":"1 4 1","pages":"245-51"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70239698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Force-induced unfolding of the focal adhesion targeting domain and the influence of paxillin binding. 力诱导的黏附靶区展开和paxillin结合的影响。

Mechanics & chemistry of biosystems : MCB Pub Date : 2004-12-01 DOI: 10.3970/MCB.2004.001.253

M. R. K. Mofrad, J. Golji, N. A. A. Rahim, R. Kamm

引用次数: 43

Forces required to initiate membrane tether extrusion from cell surface depend on cell type but not on the surface molecule. 从细胞表面启动膜系索挤压所需的力取决于细胞类型，而不取决于表面分子。

Mechanics & chemistry of biosystems : MCB Pub Date : 2004-12-01

Warren D Marcus, Rodger P McEver, Cheng Zhu

{"title":"Forces required to initiate membrane tether extrusion from cell surface depend on cell type but not on the surface molecule.","authors":"Warren D Marcus, Rodger P McEver, Cheng Zhu","doi":"","DOIUrl":"","url":null,"abstract":"When a cell adhered to another cell or substratum via surface proteins is forced to detach, lipid membrane tethers are often extruded from the cell surface before the protein bond dissociates. For example, during the inflammatory reaction leukocytes roll on the surface of activated endothelial cells. The rolling adhesion is mediated by interactions of selectins with their ligands, e.g., P-selectin glycoprotein ligand (PSGL)-1, which extrudes membrane tethers from the surfaces of both leukocytes and endothelial cells. Membrane tether extrusion has been suggested to regulate leukocyte rolling. Here we examine several factors that may affect forces required to initiate membrane tethers, or initial tether force. It was found that initial tether forces were similar regardless of the presence or absence of the cytoplasmic tail of P-selectin and regardless of whether the tethers were extruded via binding to PSGL-1 or Fcy receptors. Initial tether forces were found to depend on the cell types tested and were greatly reduced by treatment of latrunculin A, which inhibits actin polymerization. These data provide additional insights to the control of membrane tether extrusion, which should be taken into account when cellular functions such as rolling where tether extrusion plays a regulatory role are compared using different cell types expressing the same molecule.","PeriodicalId":87411,"journal":{"name":"Mechanics & chemistry of biosystems : MCB","volume":"1 4","pages":"245-51"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26097651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0