Basrah Journal of Agricultural Sciences最新文献

{"title":"Molecular Identification of Morus ssp. in Duhok Using Nuclear ITS Region and Chloroplast Matk Gene","authors":"Dalal Y. Kh. Sinjare","doi":"10.37077/25200860.2024.37.1.07","DOIUrl":"https://doi.org/10.37077/25200860.2024.37.1.07","url":null,"abstract":"Since Mulberries (Morus)is a tree species with a considerable plant variety. Molecular techniques are methods used to distinguish between species accurately, easily and quickly. This study examines a Molecular method for distinguishing different Morus species in the Duhok - Kurdistan region/ Iraq. The method is based on the use of four techniques: matK gene, the ITS region, PCR-RFLP, and SRAP markers. Twelve Morus species have been selected for this study from different region of Duhok. The ITS region's PCR result was 700 bp, but the matK gene's PCR produce was 900 bp. The same restriction site was found for all utilized cultivars when the 700bp of ITS fragment was used for PCR-RFLP with two restriction enzymes, RsaI GT/AC and HaeIII GG/CC. This study also used six combinations of SRAP markers to aid in grouping and identifying genetic similarities. The results of PCR-RFLP demonstrated an insufficient link between Morus physical appearance and genetic traits, but differences across studied cultivars could be identified using SRAP markers. Furthermore, this study demonstrated the possibility of DNA barcoding Morus cultivars, as well as additional sequence analysis and the identification of probable SNP between cultivars. ","PeriodicalId":8700,"journal":{"name":"Basrah Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140963184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Some Genetic Variation Parameters of Iraqi Sheep Population Using SSR Markers in Babylon City","authors":"Hayder R. Alnajm, Arash Javanmard","doi":"10.37077/25200860.2024.37.1.06","DOIUrl":"https://doi.org/10.37077/25200860.2024.37.1.06","url":null,"abstract":"This study focuses on designing a conservation indigenous Awassi sheep breeding program based on the analysis of genetic variation using the simple sequence repeat markers (SSR). The allele frequency distribution of six SSR markers distributed on three different chromosomes was used to determine the genetic variation among 50 Awassi sheep (15 from the north, 20 from the middle, and 15 from the south of Babylon city) that were collected from the private herds. The results showed that the RM32 marker exhibited high frequency and the most genotypes existed compared to other markers. The mean number of alleles (NA), the effective number of alleles (NE), the Shannon index (I), and the polymorphism information content (PIC) values per loci were 2.66 ± 0.81, 2.05 ± 0.87, 0.73 ± 0.42, and 0.37± 0.26 respectively. Also, the average observed (Obs_Hom), expected (Exp_Hom) homozygosity, observed (Obs_Het), expected (Exp_Het) heterozygosity, Nei’s expected heterozygosity, and inbreeding coefficient (FIS) were 0.77± 0.18, 0.56 ±0.25, 0.23 ± 0.18, 0.44 ±0.25, 0.43± 0.24, and 0.41± 0.12, respectively. The results of the Bayesian analysis revealed that all populations were homogenous there was a clear overlap between the individuals of the three distinct clusters were formed. On this basis, we conclude that the indigenous Awassi sheep in Babylon city have reasonable genetic variation.","PeriodicalId":8700,"journal":{"name":"Basrah Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140972709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Different Feeds on Growth Rate and Survival of Common Carp (Cyprinus carpio L.) Larvae","authors":"A. Mojer, A. Y. Al_Dubakel","doi":"10.37077/25200860.2024.37.1.04","DOIUrl":"https://doi.org/10.37077/25200860.2024.37.1.04","url":null,"abstract":"The present study was conducted to evaluate the growth and survival rates of common carp (Cyprinus carpio) larvae fed different diets levels in laboratory Two-day-old larvae were transferred from the University of Basrah Marine Science Center hatchery to the Abi Al-Khaseeb district's experiment laboratory. The larvae were distributed into four treatments 450 larvae each treatment (150 larvae per replicate and three replicates per treatment) at a stock density of 3.33 larvae /L. The treatments were as follows: T1 was given boiling egg yolks; T2 was given a processed diet with 45% protein; T3 was given Redi-Lac milk powder; and T4 was given ground Artemia eggs. The experiment ran for 20 days, beginning on April 17, 2022.The results showed that larvae fed on diet containing ground Artemia eggs (T4) produced  the highest final weight (39.667mg), followed by a diet   containing 45% protein processed diet (T2),  , Redi-Lac milk powder food (T3), and boiled egg yolks food (T1) produced the lowest final weight ( 27.333 and 24.667 mg, respectively) Statistical analysis revealed a significant difference (P≤0.05) in the final weight, weight gain, daily growth rate, relative growth rate and specific growth rate between T4 and T1, but not between T4 and T2 and between T4 and T3.  The survival rate showed a significant difference (P≤0.05) between T4 (0.71%) and other treatments.  Our results suggest that ground Artemia eggs is the most suitable and optimal meal for common carp fish larvae.","PeriodicalId":8700,"journal":{"name":"Basrah Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140974267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Characterization of Local Bengkulu Ambon Banana Through Chloroplast Simple Sequence Repeats (SSR) Markers","authors":"Rini Suryani, Irfan Suliansyah, W. Warnita, Aprizal Zainal, S. Sukartini","doi":"10.37077/25200860.2024.37.1.03","DOIUrl":"https://doi.org/10.37077/25200860.2024.37.1.03","url":null,"abstract":"This study aimed to explore the genetic diversity and molecular relationships of different genotypes of locally cultivated Bengkulu Ambon bananas. The investigation involved the utilization of chloroplast SSR markers and PCR techniques with 12 primers to examine 29 samples of Ambon bananas. The findings revealed monomorphic (uniform pattern) and polymorphic (varying patterns) DNA bands within the samples. In almost all samples, the monomorphic band pattern was found to be dominant at around 450 base pairs. Meanwhile, in 500 base pairs (primer 4), two different monomorphs were found.Additionally, primers 8 and 11 demonstrated multiple fragment band patterns, indicating the presence of polymorphism among the samples. The results of the dendogram analysis show that the level of similarity of all samples is relatively high. However, there are groups that represent genetic diversity. At a similarity level of 0.82 there are three clusters, four clusters at 0.85, seven clusters at 0.9 and so on. Of the 29 accessions studied, it is known that there are 11 samples that are completely genetically similar. This study provides valuable insights into the genetic diversity and relationships among locally cultivated Bengkulu Ambon bananas. The findings contribute to the existing knowledge regarding the molecular characteristics of these bananas and hold significance for conservation and breeding initiatives.","PeriodicalId":8700,"journal":{"name":"Basrah Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140971754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using Geospatial Techniques to Analysis the Impact of Climate Change on Water and Agriculture Resources: Case study Khanaqin District in Diyala, Iraq","authors":"Ahmed B. Khalaf","doi":"10.37077/25200860.2024.37.1.05","DOIUrl":"https://doi.org/10.37077/25200860.2024.37.1.05","url":null,"abstract":"The study area was classified into three categories (vegetation cover, water, and others)using four satellite images of the Landsat 8 satellite captured during March for the period 2019-2022 into. The results showed that there is a change in the climatic conditions (temperature and rainfall) for the years of the study. The average temperature increased from 12.29°C to 25.967°C from the year 2019 to 2022. The annual amount of precipitation was decreased from 469.43 mm for the year 2019 to 105.49 mm for the year 2022.this negatively changed affected the water and agricultural resources, as the amount of water storage for Lake Hamrin and Lake Al-Wand together reached to 2,314,584,000 m3 and 40,404,000 m3 for the years 2019 and 2022, respectively. This led to decrease in the vegetation area from 1587.29 km2 to 356.17 for the year 2019 km2 and 2022, respectively.","PeriodicalId":8700,"journal":{"name":"Basrah Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140976658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Quality Properties of Fresh Orange Juice through the Addition of Freeze-Dried Cassia javanica Extracts’ Coacervates","authors":"M. I. Younis, Xiaofeng Ren, Zina T. Alkanan, A. Altemimi, Khaled F. Mahmoud, Samer H. Siam, T. Abedelmaksoud","doi":"10.37077/25200860.2024.37.1.02","DOIUrl":"https://doi.org/10.37077/25200860.2024.37.1.02","url":null,"abstract":"This study investigated the impact of freeze-dried coacervates at various concentrations (0.5, 1, and 1.5%) on physicochemical and microbiological properties of orange juice. Either 60% ethanol freeze-dried coacervates (EFC) or absolute ethanol freeze-dried coacervates (AFC) were used. The addition of EFC or AFC to orange juice did not significantly (p<0.05) affect pH, viscosity, or electric conductivity, as pH was unchanged for a 0.5% concentration and slightly increased from 3.99 to 4.01 at 1% and 1.5% concentrations. After adding AFC and EFC, viscosity were 52, 53, and 53 mPas at 0.5, 1, and 1.5% concentrations, respectively. Electric conductivity increased from 0.278 s.m-1 (control) to 0.334, 0.347, and 0.375 s.m-1 at 0.5, 1, and 1.5% concentrations after adding EFC, while were 0.325, 0.335, and 0.373 s.m-1 at the same concentration after adding AFC, respectively. However, after adding EFC, total phenolic content increased to 77.25, 115.96, and 154.95 mg.100mL-1, total flavonoid content (TFC)increased to 34.76, 52.18, and 69.73 mg.100mL-1, and antioxidant activity (AA) enhanced to 70.36, 74.36, and 79.58% at concentrations 0.5, 1, and 1.5%, respectively. Also, after adding AFC, total phenolic content increased to 79.26, 117.78, and 156.25 mg.100mL-1, TFC increased to 35.67, 53.00, and 70.31 mg.100mL-1, and AA enhanced to 71.65, 75.84, and 81.21% at concentrations 0.5, 1, and 1.5%, respectively. At concentrations 0.5, 1, and 1.5%, EFC decreased total plate count (TPC) to 2.12, 2.02, and 1.78 log cfu.mL-1, respectively and mold and yeast counts decreased to 1.5, 1.35, and 1.1 log cfu.mL-1, respectively. Also, adding AFC caused TPC to decrease to 2.18, 2.04, and 1.84 log cfu.mL-1, respectively and mold and yeast count decreased to 1.53, 1.33, and 1.12 log cfu.mL-1, respectively. Overall, the results indicate that adding EFC or AFC to fresh orange juice can enhance its nutritional and microbiological qualities without degrading its sensory qualities.","PeriodicalId":8700,"journal":{"name":"Basrah Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140986808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Managing Dust Mite Oligonychus afrasiaticus (McGregor) (Acari: Tetranchidae) Infesting Date Palm Orchards By Using Lemongrass Extract and Nanosulfur","authors":"Hazim M. Ali, Khalid A. Fhaid, Khairullah M. Awad","doi":"10.37077/25200860.2024.37.1.01","DOIUrl":"https://doi.org/10.37077/25200860.2024.37.1.01","url":null,"abstract":"The dust mite Oligonychus afrasiaticus (McGregor 1939) (Acari: Tetranychidae) poses a significant threat to date palm Phoenix dactylifera L. productivity in Iraq. To address this issue, a field study was conducted in a private date palm orchard in Al-Zubair district, Basrah province, Iraq, to assess the efficacy of some treatments in controlling the dust mite population on date palm fruits. The treatments included lemongrass extract, nanosulfur, a combination of lemongrass extract and nanosulfur, liquid sulfur, and a distilled water as control treatment. Treatments were applied by spraying on leaves and fruits at three dates: five weeks, eight weeks, and eleven weeks after pollination. Dust mite mortality rates were measured at intervals of 1, 3, 5, and 7 days after treatment application. Results revealed that the second date, eight weeks after pollination, exhibited the highest mortality rate of 76.77%, significantly outperforming other dates. Among the treatments, liquid sulfur showed the highest efficiency, with an 87.83% mortality rate, followed closely by the combination of lemongrass extract and nanosulfur (84.14%). Nanosulfur alone recorded a mortality rate of 79.26%, while lemongrass extract alone showed a mortality rate of 49.15%. The mortality rate increased over time, reaching 80.79% after 7 days and 65.76% after 1 day. In conclusion, liquid sulfur emerged as the most effective treatment for controlling the dust mite population on date palm fruits. The second date, eight weeks after pollination, was the optimal time for treatment application. These findings offer valuable insights into sustainable and environmentally friendly pest control strategies for date palm cultivation in Iraq.","PeriodicalId":8700,"journal":{"name":"Basrah Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140985862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Planting Dates on Stability of New Rice Genotypes Traits Grown in Iraqi Conditions","authors":"S. H. Abbas","doi":"10.37077/25200860.2023.36.2.01","DOIUrl":"https://doi.org/10.37077/25200860.2023.36.2.01","url":null,"abstract":"Selecting the appropriate date for planting rice can be one of the viable solutions for proper growth and development of rice to avoid unsuitable conditions that can lead to lower productivity. Two experiments were conducted in the field of Al-Mishkhab Rice Research Station in Najaf City / Iraq during 2020 and 2021. In each season, eight genotypes used and two germination dates (15 and 30 Jun). After 20 days, seedlings were transferred to the field and planted on 5th and 20th of July respectively. The experiment was based on a three-blocks in RCBD design to study the performance of eight genotypes at two planting dates in two seasons. Results showed significant differences for genotypes and planting dates for all traits, while seasons showed significant differences only for the number of days to 50% flowering and panicle length. The values of heritability in the broad sense (h2BS %) were medium for all studied traits, which in turn led to an increase in the values of expected genetic advance as a percentage of the general mean (GA %), which was slightly high for grain yield (13.66 %). The genotypes showed a significant response to the late sowing date of 20th July compared to the early date of 5th July in the two growing seasons for most of the studied traits, including grain yield. The stability of the genotypes in four environments was highly variable. Genotype T93 had the most stable grain yield and the highest 1000-grain weight, while Forat1 showed a response to late sowing. Furthermore, the Forat1 responded to suitable environmental conditions of the number of days to 50% flowering, 1000-grain weight, and grain yield. Therefore, the genotypes which have superiority and stability over various environments could be successfully used as crucial material in the future breeding programs.","PeriodicalId":8700,"journal":{"name":"Basrah Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79435743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using of Conspicuous of Body Angularities Type Traits to Milk Yields as Dairy Cattle Selection Preferences","authors":"S. Prabowo, M. Garip","doi":"10.37077/25200860.2023.36.1.19","DOIUrl":"https://doi.org/10.37077/25200860.2023.36.1.19","url":null,"abstract":"Numerous papers have been published on body cattle angularities subject in a few backward decades. However, the preeminent body angularities to the milk yield are unstipulated assertively. Hence, the current odyssey was to determine the transcendence body angular of dairy cattle interrelated with the milk yield for selection preferences. In total, 121 head of Holstein cows and seven reputable cattle body angularities were engaged as samples and measured variables for investigation. The software R version 4.2.1 and RStudio was operated simultaneously to facilitate statistical analysis. Later, the principal components (PCA), correlation, and regression analysis were carried out in that order. The PCA specified the thurl angle (TLA), hock side view angle (HSA), hock back views angle (HBA), and fore udder angle (FUA) as crucial factors of body cattle angularities. Then, the correlation analysis appointed HBA and TLA in series as the best trait related to milk yields. The regression analysis was merely entrusted to the HBA as a factor for prognosticating milk yield potency. Thus, the upshot of the ongoing exploration prompted the HBA as the main priority for milk yield selection preferences, followed by TLA. Both were usable on the calf, heifer, and cow selection scheme but should be enforced regularly.","PeriodicalId":8700,"journal":{"name":"Basrah Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83081743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Foliar NPK Application on Growth, Yield and Nutrient Content of Sweet Corn Grown on Rengam Series Soil","authors":"Bilal A. Mohammed, M. M. Khandaker, Adzemi M. Arshad, Nur Fatihah H. Nudin, A. Majrashi, K. S. Mohd","doi":"10.37077/25200860.2023.36.1.20","DOIUrl":"https://doi.org/10.37077/25200860.2023.36.1.20","url":null,"abstract":"The present study was carried out to evaluate the effects of different rates of macronutrients as a foliar spray on the growth performance, yield, and nutrient content of sweet corn grown in the Rengam soil series. The treatments consisted of five rates of macronutrients as a foliar fertilizer at 0, 25, 50, 75, and 100 % NPK. Foliar NPK was applied 25 and 50 days after sowing to the sweet corn seedlings. The results showed that fresh cob weight, cob number, flowering, and dry matter yield of sweet corn significantly increased at the rate of 75%, and 100% of NPK foliar fertilizers. The macro and micronutrient concentrations in ear leaf, mature leaves, stem, cob, and flowers of 75 and 100% NPK treated corn were significantly increased over the control plants. The macronutrient content in the whole plant was also significantly higher at 75% and 100% NPK treatments. Fe and Mn contents in the whole plant were also the highest in 75% and 100% NPK treatments. Macronutrient concentration in ear leaf and whole corn plants significantly correlated with the fresh cob yield of corn. It is concluded that foliar application of N, P, and K macronutrients (75 to 100% NPK) enhanced the yield and quality of sweet corn.","PeriodicalId":8700,"journal":{"name":"Basrah Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86061507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}