Experimental diabesity research最新文献

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Noninvasive orthogonal polarization spectral imaging as applied to microvascular studies in mice. 无创正交偏振光谱成像在小鼠微血管研究中的应用。
Experimental diabesity research Pub Date : 2004-07-01 DOI: 10.1080/15438600490486859
P Nivoit, A M Chevrier, M Lagarde, C Renaudin, N Wiernsperger
{"title":"Noninvasive orthogonal polarization spectral imaging as applied to microvascular studies in mice.","authors":"P Nivoit,&nbsp;A M Chevrier,&nbsp;M Lagarde,&nbsp;C Renaudin,&nbsp;N Wiernsperger","doi":"10.1080/15438600490486859","DOIUrl":"https://doi.org/10.1080/15438600490486859","url":null,"abstract":"<p><p>In vivo observations of the mouse microcirculation can hardly be performed due to technical difficulties, limiting the knowledge that could be obtained from gene manipulated mice models. The aim of the present study was to check the applicability of a novel optical system, the orthogonal polarization spectral technology, to study the mouse microcirculation. In anaesthetized mice, the spinotrapezius muscle microcirculation was observed in situ. The diameter of precapillary arterioles was measured before and after a pharmacological or hormonal stimulation. High-contrast images of the muscle microcirculation were obtained and significant vasodilatation of arterioles was observed after topical applications of acetylcholine, sodium nitroprusside, and insulin. As compared to conventional techniques, orthogonal polarization spectral imaging makes it possible to assess and study microvascular beds in mice, which were inaccessible until now, allowing the use of gene manipulated mice to investigate, for example, the mechanisms involved in the development of diabetic microangiopathy.</p>","PeriodicalId":86960,"journal":{"name":"Experimental diabesity research","volume":"5 3","pages":"211-7"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15438600490486859","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24785638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Improved experimental procedures for achieving efficient germ line transmission of nonobese diabetic (NOD)-derived embryonic stem cells. 改进实验程序,实现非肥胖糖尿病(NOD)来源的胚胎干细胞的有效生殖系传播。
Experimental diabesity research Pub Date : 2004-07-01 DOI: 10.1080/15438600490486877
Satoko Arai, Christina Minjares, Seiho Nagafuchi, Toru Miyazaki
{"title":"Improved experimental procedures for achieving efficient germ line transmission of nonobese diabetic (NOD)-derived embryonic stem cells.","authors":"Satoko Arai,&nbsp;Christina Minjares,&nbsp;Seiho Nagafuchi,&nbsp;Toru Miyazaki","doi":"10.1080/15438600490486877","DOIUrl":"https://doi.org/10.1080/15438600490486877","url":null,"abstract":"<p><p>The manipulation of a specific gene in NOD mice, the best animal model for insulin-dependent diabetes mellitus (IDDM), must allow for the precise characterization of the functional involvement of its encoded molecule in the pathogenesis of the disease. Although this has been attempted by the cross-breeding of NOD mice with many gene knockout mice originally created on the 129 or C57BL/6 strain background, the interpretation of the resulting phenotype(s) has often been confusing due to the possibility of a known or unknown disease susceptibility locus (e.g., Idd locus) cosegregating with the targeted gene from the diabetes-resistant strain. Therefore, it is important to generate mutant mice on a pure NOD background by using NOD-derived embryonic stem (ES) cells. By using the NOD ES cell line established by Nagafuchi and colleagues in 1999 (FEBS Lett., 455, 101-104), the authors reexamined various conditions in the context of cell culture, DNA transfection, and blastocyst injection, and achieved a markedly improved transmission efficiency of these NOD ES cells into the mouse germ line. These modifications will enable gene targeting on a \"pure\" NOD background with high efficiency, and contribute to clarifying the physiological roles of a variety of genes in the disease course of IDDM.</p>","PeriodicalId":86960,"journal":{"name":"Experimental diabesity research","volume":"5 3","pages":"219-26"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15438600490486877","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24784807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Effect of fidarestat and alpha-lipoic acid on diabetes-induced epineurial arteriole vascular dysfunction. 非达司他和硫辛酸对糖尿病诱导的神经外小动脉血管功能障碍的影响。
Experimental diabesity research Pub Date : 2004-04-01 DOI: 10.1080/15438600490277824
M A Yorek, L J Coppey, J S Gellett, E P Davidson, D D Lund
{"title":"Effect of fidarestat and alpha-lipoic acid on diabetes-induced epineurial arteriole vascular dysfunction.","authors":"M A Yorek,&nbsp;L J Coppey,&nbsp;J S Gellett,&nbsp;E P Davidson,&nbsp;D D Lund","doi":"10.1080/15438600490277824","DOIUrl":"https://doi.org/10.1080/15438600490277824","url":null,"abstract":"<p><p>In the present study, the authors examined whether treating streptozotocin-induced diabetic rats with the combination of alpha-lipoic acid and fidarestat, an aldose reductase inhibitor, can promote the formation of dihydrolipoic acid in diabetic animals and thereby enhance the efficacy of alpha-lipoic acid as monotherapy toward preventing diabetic vascular and neural dysfunction. Treating diabetic rats with the combination of 0.25% alpha-lipoic acid (in the diet) and fidarestat (3 mg/kg body weight) prevented the diabetes-induced slowing of motor nerve conduction velocity and endoneurial blood flow. This therapy also significantly improved acetylcholine-mediated vasodilation in epineurial arterioles of the sciatic nerve compared to nontreated diabetic rats. Treating diabetic rats with 0.25% alpha-lipoic acid and fidarestat (3 mg/kg body weight) was equally or more effective in preventing vascular and neural dysfunction than was monotherapy of diabetic rats with higher doses of alpha-lipoic acid or fidarestat. Treating diabetic rats with the combination of 0.25% alpha-lipoic acid and fidarestat (3 mg/kg body weight) significantly improved several markers of oxidative stress and increased the serum levels of both alpha-lipoic acid and dihydrolipoic acid. These studies suggest that combination therapy consisting of alpha-lipoic acid and fidarestat may be more efficacious in preventing diabetes-induced vascular and neural dysfunction in peripheral tissue compared to monotherapy, which requires higher doses to be equally effective. The effect of this combination therapy may in part be due to the increased production and/or level of dihydrolipoic acid.</p>","PeriodicalId":86960,"journal":{"name":"Experimental diabesity research","volume":"5 2","pages":"123-35"},"PeriodicalIF":0.0,"publicationDate":"2004-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15438600490277824","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24572299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
Hypoglycemic effect of macrocyclic binuclear oxovanadium (IV) complex on streptozotocin-induced diabetic rats. 大环双核钒氧配合物对链脲佐菌素诱导的糖尿病大鼠的降糖作用。
Experimental diabesity research Pub Date : 2004-04-01 DOI: 10.1080/15438600490277842
B Ramachandran, D Sathish Sekar, M Kandaswamy, V Narayanan, S Subramanian
{"title":"Hypoglycemic effect of macrocyclic binuclear oxovanadium (IV) complex on streptozotocin-induced diabetic rats.","authors":"B Ramachandran,&nbsp;D Sathish Sekar,&nbsp;M Kandaswamy,&nbsp;V Narayanan,&nbsp;S Subramanian","doi":"10.1080/15438600490277842","DOIUrl":"https://doi.org/10.1080/15438600490277842","url":null,"abstract":"<p><p>Though vanadium complexes mimic the action of insulin, owing to their toxicity, research is still in progress for a new vanadium complex with maximum efficacy at low concentration and without any side effects. A novel macrocyclic binuclear oxovanadium complex was synthesized, its composition and structure were confirmed by spectral studies and its efficacy was studied in streptozotocin-induced diabetic rats over a period of 30 days. The oral administration of the complex normalizes the blood glucose level in the diabetic rats and also maintains normoglycemia after a glucose load. The biochemical studies revealed that the complex is not toxic to the system. The nontoxic nature of this complex may be due to the presence of the vanadyl ions in an intact form. The study highlights the nontoxic and hypoglycemic effects of the new macrocyclic binuclear oxovanadium complex.</p>","PeriodicalId":86960,"journal":{"name":"Experimental diabesity research","volume":"5 2","pages":"137-42"},"PeriodicalIF":0.0,"publicationDate":"2004-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15438600490277842","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24572300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Review: pancreatic beta-cell neogenesis revisited. 回顾:胰腺β细胞新生的重新审视。
Experimental diabesity research Pub Date : 2004-04-01 DOI: 10.1080/15438600490455079
Maryline Paris, Cécile Tourrel-Cuzin, Cédric Plachot, Alain Ktorza
{"title":"Review: pancreatic beta-cell neogenesis revisited.","authors":"Maryline Paris,&nbsp;Cécile Tourrel-Cuzin,&nbsp;Cédric Plachot,&nbsp;Alain Ktorza","doi":"10.1080/15438600490455079","DOIUrl":"https://doi.org/10.1080/15438600490455079","url":null,"abstract":"<p><p>Beta-cell neogenesis triggers the generation of new beta-cells from precursor cells. Neogenesis from duct epithelium is the most currently described and the best documented process of differentiation of precursor cells into beta-cells. It is contributes not only to beta-cell mass expansion during fetal and nonatal life but it is also involved in the maintenance of the beta-cell mass in adults. It is also required for the increase in beta-cell mass in situations of increase insulin demand (obesity, pregnancy). A large number of factors controlling the differentiation of beta-cells has been identified. They are classified into the following main categories: growth factors, cytokine and inflammatory factors, and hormones such as PTHrP and GLP-1. The fact that intestinal incretin hormone GLP-1 exerts a major trophic role on pancreatic beta-cells provides insights into the possibility to pharmacologically stimulate beta-cell neogenesis. This could have important implications for the of treatment of type 1 and type 2 diabetes. Transdifferentiation, that is, the differentiation of already differentiated cells into beta-cells, remains controversial. However, more and more studies support this concept. The cells, which can potentially \"transdifferentiate\" into beta-cells, can belong to the pancreas (acinar cells) and even islets, or originate from extra-pancreatic tissues such as the liver. Neogenesis from intra-islet precursors also have been proposed and subpopulations of cell precursors inside islets have been described by some authors. Nestin positive cells, which have been considered as the main candidates, appear rather as progenitors of endothelial cells rather than beta-cells and contribute to angiogenesis rather than neogenesis. To take advantage of the different differentiation processes may be a direction for future cellular therapies. Ultimately, a better understanding of the molecular mechanisms involved in beta-cell neogenesis will allow us to use any type of differentiated and/or undifferentiated cells as a source of potential cell precursors.</p>","PeriodicalId":86960,"journal":{"name":"Experimental diabesity research","volume":"5 2","pages":"111-21"},"PeriodicalIF":0.0,"publicationDate":"2004-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15438600490455079","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24572298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 46
Increased expression of tissue factor and receptor for advanced glycation end products in peripheral blood mononuclear cells of patients with type 2 diabetes mellitus with vascular complications. 2型糖尿病伴血管并发症患者外周血单个核细胞组织因子和晚期糖基化终产物受体的表达升高
Experimental diabesity research Pub Date : 2004-04-01 DOI: 10.1080/15438600490424325
A E Buchs, A Kornberg, M Zahavi, D Aharoni, C Zarfati, M J Rapoport
{"title":"Increased expression of tissue factor and receptor for advanced glycation end products in peripheral blood mononuclear cells of patients with type 2 diabetes mellitus with vascular complications.","authors":"A E Buchs,&nbsp;A Kornberg,&nbsp;M Zahavi,&nbsp;D Aharoni,&nbsp;C Zarfati,&nbsp;M J Rapoport","doi":"10.1080/15438600490424325","DOIUrl":"https://doi.org/10.1080/15438600490424325","url":null,"abstract":"<p><p>The aim of the study was to determine the correlation between the expression of tissue factor (TF) and the receptor for advanced glycation end products (RAGEs) and vascular complications in patients with longstanding uncontrolled type 2 diabetes (T2D). TF and RAGE mRNAs as well as TF antigen and activity were investigated in 21 T2D patients with and without vascular complications. mRNA expression was assessed by reverse transcriptase-polymerase chain reaction (RT-PCR) in nonstimulated and advanced glycation end product (AGE) albumin-stimulated peripheral blood mononuclear cells (PBMCs). TF antigen expression was determined by enzyme-linked immunosorbent assay (ELISA) and TF activity by a modified prothrombin time assay. Basal RAGE mRNA expression was 0.2 +/- 0.06 in patients with complications and 0.05 +/- 0.06 patients without complications (P =.004). Stimulation did not cause any further increase in either group. TF mRNA was 0.58 +/- 0.29 in patients with complications and 0.21 +/- 0.18 in patients without complications (P =.003). Stimulation resulted in a nonsignificant increase in both groups. Basal TF activity (U/10(6) PBMCs) was 18.4 +/- 13.2 in patients with complications and 6.96 +/- 5.2 in patients without complications (P =.003). It increased 3-fold in both groups after stimulation (P =.001). TF antigen (pg/10(6) PBMCs) was 33.7 +/- 28.6 in patients with complications, 10.4 +/- 7.8 in patients without complications (P =.02). Stimulation tripled TF antigen in both groups of patients (P =.001). The RAGE/TF axis is up-regulated in T2D patients with vascular complications as compared to patients without complications. This suggests a role for this axis in the pathogenesis of vascular complications in T2D.</p>","PeriodicalId":86960,"journal":{"name":"Experimental diabesity research","volume":"5 2","pages":"163-9"},"PeriodicalIF":0.0,"publicationDate":"2004-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15438600490424325","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24572192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Review: peroxisome proliferator-activated receptor-gamma and its role in the development and treatment of diabetes. 综述:过氧化物酶体增殖物激活受体- γ及其在糖尿病发生和治疗中的作用。
Experimental diabesity research Pub Date : 2004-04-01 DOI: 10.1080/15438600490451668
Todd Leff, Suresh T Mathews, Heidi S Camp
{"title":"Review: peroxisome proliferator-activated receptor-gamma and its role in the development and treatment of diabetes.","authors":"Todd Leff,&nbsp;Suresh T Mathews,&nbsp;Heidi S Camp","doi":"10.1080/15438600490451668","DOIUrl":"https://doi.org/10.1080/15438600490451668","url":null,"abstract":"<p><p>Since its identification as the receptor for antidiabetic thiazolidinedione drugs, peroxisome proliferator-activated receptor-gamma (PPARgamma) has been the focus of pharmaceutical drug discovery programs directed toward finding better drugs for the treatment of diabetes, as well as the object of basic research aimed at understanding its role in the regulation of metabolism. We now understand a great deal about the crucial role that PPARgamma plays in adipocyte differentiation and development, and are rapidly gaining knowledge about the role of the receptor in the regulation of metabolism. However, many crucial aspects of the molecular mechanism by which modulation of PPARgamma activity affects insulin resistance and glucose homeostasis are still not clearly understood. Here the authors review the current status of PPARgamma research, with an emphasis on its role in the causes and treatment of type 2 diabetes.</p>","PeriodicalId":86960,"journal":{"name":"Experimental diabesity research","volume":"5 2","pages":"99-109"},"PeriodicalIF":0.0,"publicationDate":"2004-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15438600490451668","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24572297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Cross-linking in collagen by nonenzymatic glycation increases the matrix stiffness in rabbit achilles tendon. 非酶糖基化胶原交联增加兔跟腱基质刚度。
Experimental diabesity research Pub Date : 2004-04-01 DOI: 10.1080/15438600490277860
G Kesava Reddy
{"title":"Cross-linking in collagen by nonenzymatic glycation increases the matrix stiffness in rabbit achilles tendon.","authors":"G Kesava Reddy","doi":"10.1080/15438600490277860","DOIUrl":"https://doi.org/10.1080/15438600490277860","url":null,"abstract":"<p><p>Nonenzymatic glycation of connective tissue matrix proteins is a major contributor to the pathology of diabetes and aging. Previously the author and colleagues have shown that nonenzymatic glycation significantly enhances the matrix stability in the Achilles tendon (Reddy et al., 2002, Arch. Biochem. Biophys., 399, 174-180). The present study was designed to gain further insight into glycation-induced collagen cross-linking and its relationship to matrix stiffness in the rabbit Achilles tendon. The glycation process was initiated by incubating the Achilles tendons (n = 6) in phosphate-buffered saline containing ribose, whereas control tendons (n = 6) were incubated in phosphate-buffered saline without ribose. Eight weeks following glycation, the biomechanical attributes as well as the degree of collagen cross-linking were determined to examine the potential associations between matrix stiffness and molecular properties of collagen. Compared to nonglycated tendons, the glycated tendons showed increased maximum load, stress, strain, Young's modulus of elasticity, and toughness indicating that glycation increases the matrix stiffness in the tendons. Glycation of tendons resulted in a considerable decrease in soluble collagen content and a significant increase in insoluble collagen and pentosidine. Analysis of potential associations between the matrix stiffness and degree of collagen cross-linking showed that both insoluble collagen and pentosidine exhibited a significant positive correlation with the maximum load, stress, and strain, Young's modulus of elasticity, and toughness (r values ranging from.61 to.94) in the Achilles tendons. However, the soluble collagen content present in neutral salt buffer, acetate buffer, and acetate buffer containing pepsin showed an inverse relation with the various biomechanical attributes tested (r values ranging from.22 to.84) in the Achilles tendons. The results of the study demonstrate that glycation-induced collagen cross-linking is directly associated with the increased matrix stiffness and other mechanical attributes of the tendon.</p>","PeriodicalId":86960,"journal":{"name":"Experimental diabesity research","volume":"5 2","pages":"143-53"},"PeriodicalIF":0.0,"publicationDate":"2004-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15438600490277860","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24572302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 216
Activities of antioxidant scavenger enzymes (superoxide dismutase and glutathione peroxidase) in erythrocytes in adult women with and without type II diabetes. 2型糖尿病的成年女性红细胞中抗氧化清除率(超氧化物歧化酶和谷胱甘肽过氧化物酶)的活性
Experimental diabesity research Pub Date : 2004-04-01 DOI: 10.1080/15438600490451659
Eli Carmeli, Raymond Coleman, Yitshal N Berner
{"title":"Activities of antioxidant scavenger enzymes (superoxide dismutase and glutathione peroxidase) in erythrocytes in adult women with and without type II diabetes.","authors":"Eli Carmeli,&nbsp;Raymond Coleman,&nbsp;Yitshal N Berner","doi":"10.1080/15438600490451659","DOIUrl":"https://doi.org/10.1080/15438600490451659","url":null,"abstract":"<p><p>It is widely believed that oxidative stress plays an important role in the pathogenesis of type II diabetes. The present study was undertaken to examine the functioning of two antioxidant scavenger enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), in erythrocytes in a population of healthy aging adult women compared with a similar population with type II diabetes. Blood samples were examined from 42 female adult healthy subjects at different ages and from 59 female patients with type II diabetes. A significant increase in SOD activities was correlated with aging in erythrocytes of the healthy control subjects (r =.550, P =.001); however, this correlation was not found in subjects with type II diabetes (r =.250, P <.07). A trend showing a reduction in glutathione peroxidase activities was demonstrated with aging (r = -.331, P =.228); however, this trend was not found in diabetic subjects (r =.031, P <.820). The results indicate a possible imbalance in the antioxidant system in erythrocytes of aging adult women, which is even more pronounced in cases of type II diabetes. This study may indicate possible therapeutic treatment or preventive measures to limit oxidative damage and reduce complications of diabetes.</p>","PeriodicalId":86960,"journal":{"name":"Experimental diabesity research","volume":"5 2","pages":"171-5"},"PeriodicalIF":0.0,"publicationDate":"2004-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15438600490451659","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24572191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Nicotinamide effects oxidative burst activity of neutrophils in patients with poorly controlled type 2 diabetes mellitus. 烟酰胺对控制不良的2型糖尿病患者中性粒细胞氧化爆发活性的影响。
Experimental diabesity research Pub Date : 2004-04-01 DOI: 10.1080/15438600490424244
Zeynep Osar, Tülay Samanci, Gülderen Yanikkaya Demirel, Taner Damci, Hasan Ilkova
{"title":"Nicotinamide effects oxidative burst activity of neutrophils in patients with poorly controlled type 2 diabetes mellitus.","authors":"Zeynep Osar,&nbsp;Tülay Samanci,&nbsp;Gülderen Yanikkaya Demirel,&nbsp;Taner Damci,&nbsp;Hasan Ilkova","doi":"10.1080/15438600490424244","DOIUrl":"https://doi.org/10.1080/15438600490424244","url":null,"abstract":"<p><p>Neutrophil functions are impaired in patients with diabetes mellitus. Bacterial phagocytosis and oxidative burst activity are reduced at high glucose concentrations in diabetic patients. Defects in neutrophil oxidative burst capacity are of multifactorial origin in diabetes mellitus and correlate with glucose levels. It has been reported that neutrophil NADPH oxidase activity is impaired and superoxide production is reduced in diabetic patients with or without any infections. Nicotinamide is a vitamin B3 derivative and a NAD precursor with immunomodulatory effects. In vitro studies demonstrated that nicotinamide increases NAD and NADH content of beta cells. The authors hypothesized that nicotinamide may restore the impaired oxidative burst capacity of neutrophils in diabetic patients by increasing the NADH content as an electron donor and possibly through NADPH oxidase activity of the cell. In order to test the hypothesis, this placebo-controlled and open study was designed to evaluate neutrophil functions in infection-free poorly controlled type 2 diabetic patients as compared to healthy subjects and assess the effects of nicotinamide on neutrophil phagocytosis as well as oxidative burst activity. Thirty patients with type 2 diabetes mellitus were enrolled in the study. Sixteen were females and 14 were males, with a mean age 58 +/- 10. All patients were on sulphonylurea treatment and their hemoglobin A(1c) (HbA(1c)) levels were above 7.5%. The control group consisted of 10 voluntary healthy subjects. Diabetic and control subjects were not significantly different in terms of age, body mass index (BMI), leucocyte and neutrophil counts, C-reactive protein (CRP) level, and erythrocyte sedimentation rate (ESR), but HbA(1c) and fasting glucose levels were significantly higher in patients with diabetes mellitus. Phagocytic activity and respiratory burst indexes were measured by flow cytometric analyses as previously described by Rothe and Valet (Methods Enzyml., 233, 539-548, 1994) and compared in diabetic subjects and healthy controls. Diabetic patients were grouped to receive either 50 mg/kg oral nicotinamide (n = 15) or placebo (n = 15) for a period of 1 month. The 2 groups did not differ in terms of treatment, frequency of hypertension, BMI, diabetes duration, age, fasting plasma glucose (FPG), HbA(1c), CRP, ESR, polymorphonuclear leukocyte (PNL) and neutrophil counts. Neutrophil functions were reassessed after the treatment period. Phagocytic activity represented as indexes were lower in diabetic patients when compared to healthy subjects, but the differences were not statistically significant (P >.05). Patients with diabetes mellitus had significantly lower oxidative burst indexes when compared to healthy controls (P values <.05). In diabetic patients, a negative correlation between neutrophil functions and HbA(1c) was found which was not statistically significant (P values >.05). Phagocytic indexes were similar in nicotinamide and placebo ","PeriodicalId":86960,"journal":{"name":"Experimental diabesity research","volume":"5 2","pages":"155-62"},"PeriodicalIF":0.0,"publicationDate":"2004-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15438600490424244","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24572189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 40
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