H. Adekola, Kareem A. Wahab, Omotayo E. Odunsi, Tobiloba A. Abesin, Oluwaseun A. Oyesanya
{"title":"Current diagnostics and biomarkers for arboviral infections (a review on Dengue, Zika, West Nile and Chikungunya viruses)","authors":"H. Adekola, Kareem A. Wahab, Omotayo E. Odunsi, Tobiloba A. Abesin, Oluwaseun A. Oyesanya","doi":"10.36233/0507-4088-211","DOIUrl":"https://doi.org/10.36233/0507-4088-211","url":null,"abstract":"Arboviral infections, transmitted to humans primarily through arthropod vectors, constitute a significant global health threat. Arboviruses, such as Dengue, Zika, Chikungunya, and West Nile viruses, continue to cause widespread outbreaks, necessitating advanced diagnostic tools. Emerging technologies including Lab On A Chip (LOC), Lab On A Disc (LOAD), Microfluidic Paper-Based Analytical Devices (µPADS), Lateral Flow Devices, CRISPR-CAS 12/13, Quartz crystal microbalance (QCM), and Nano-Technology are evaluated for their potential to enhance arboviral diagnosis, offering rapid, accurate, and point-of-care solutions. Furthermore, the identification of robust biomarkers, including Inflammatory Cytokines, Antibodies, Endothelial Activation Products and Indicators of Tissue or Organ Damage, is crucial for improving the understanding of disease pathogenesis, prognosis, and treatment response. A comprehensive analysis of potential diagnostics and biomarkers for arboviral infections sheds light on the evolving strategies to combat these medically significant diseases, ultimately contributing to more effective surveillance, diagnosis and management worldwide.","PeriodicalId":86540,"journal":{"name":"Problems of virology","volume":"23 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140243983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Why may the 21st century become the «century of pandemics»? А question for discussion","authors":"Felix I. Ershov","doi":"10.36233/0507-4088-227","DOIUrl":"https://doi.org/10.36233/0507-4088-227","url":null,"abstract":"In the first 20 years of the 21st century, almost annual outbreaks of viral infections were recorded. It is becoming increasingly clear that until humanity changes its destructive attitude towards nature, epidemics of deadly diseases will continue to occur. A question arises for discussion: is the existing arsenal of antiviral agents sufficient to withstand the current unfavorable socio-economic situation in the world?","PeriodicalId":86540,"journal":{"name":"Problems of virology","volume":"35 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140245017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Glotov, A. Yuzhakov, T. Glotova, A. V. Nefedchenko, S. V. Koteneva, A. Komina, Elena V. Zhukova
{"title":"Occurrence in sick animals and genetic heterogeneity of Siberian isolates of bovine respiratory syncytial virus (Pneumoviridae: Orthopneumovirus; BRSV) identified in the territories of the Ural, Siberian Federal District and the Republic of Kazakhstan","authors":"A. Glotov, A. Yuzhakov, T. Glotova, A. V. Nefedchenko, S. V. Koteneva, A. Komina, Elena V. Zhukova","doi":"10.36233/0507-4088-216","DOIUrl":"https://doi.org/10.36233/0507-4088-216","url":null,"abstract":"Introduction. Bovine respiratory syncytial virus (Pneumoviridae: Orthornavirae, Orthopneumovirus; Bovine orthopneumovirus, Bovine respiratory syncytial virus, BRSV) is one of causative agents of respiratory diseases in animals. The study of the occurrence and genetic diversity of this pathogen is of particular importance. \u0000Objective. To study the frequency of virus in animals using RT-PCR and genetic heterogeneity of isolates based on determining the complete nucleotide sequence of glycoprotein G gene. \u0000Materials and methods. A 381-bp region of glycoprotein F gene was used for identification of virus genome, while complete nucleotide sequences of G gene were used for phylogenetic analysis. Phylogenetic trees were constructed using the maximum likelihood method in MEGA 7.0 software. \u0000Results. During outbreaks of respiratory diseases, BRSV RNA was detected in animals of all ages in samples of lungs, nasal secretions, pulmonary lymph nodes. Complete nucleotide sequences of glycoprotein G gene, 771 bp in length were obtained for five isolates and 789 bp in length ‒ for two isolates. Nucleotide similarity between them was 87–100%. Phylogenetic analysis assigned the isolates to subgroups II and III, each of which included two isolates. A separate clade formed by K18 isolate from animals imported from Canada and sequences from vaccines containing the attenuated «375» strain. \u0000Conclusion. The virus genome was identified in cows and heifers (20.0 and 14.3%), in calves up 1 month of age (3.05%), and in calves from 1 to 6 months of age (6.7%). Complete G gene nucleotide sequence analysis is a useful tool for studying the molecular epidemiology of BRSV on particular territories.","PeriodicalId":86540,"journal":{"name":"Problems of virology","volume":"12 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140244440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. D. Chanyshev, N. V. Vlasenko, G. Roev, I.A. Kotov, Albina G. Glushchenko, V. V. Makashova, Kamil F. Khafizov, V. Akimkin
{"title":"NGS amplification panel for HBV (Hepadnaviridae: Orthohepadnavirus) sequencing","authors":"M. D. Chanyshev, N. V. Vlasenko, G. Roev, I.A. Kotov, Albina G. Glushchenko, V. V. Makashova, Kamil F. Khafizov, V. Akimkin","doi":"10.36233/0507-4088-212","DOIUrl":"https://doi.org/10.36233/0507-4088-212","url":null,"abstract":"Introduction. Hepatitis B virus (HBV) remains a pressing global public health concern. The clinical course of the disease, particularly its tendency towards chronicity and response to therapy, is significantly influenced by the HBV genotype and specific mutations. There is an imperative need for a straightforward, highly sensitive, and dependable method for whole genome sequencing of HBV. \u0000Objective. Development and testing of an amplification panel for HBV whole-genome sequencing. \u0000Materials and methods. We introduce an NGS amplification panel designed for genome sequencing of HBV on the Illumina platform. A panel consisting of 54 primers, divided into 2 pools and amplifying overlapping regions of the HBV genome up to 300 bp in length, was tested on 246 HBV DNA samples. \u0000Results. The studied samples represented a genotypic diversity of the virus, with a pronounced predominance of the genotype specific to the Moscow region: 216, 27, 2, and 1 sample were identified as genotype D, A, B, and E, respectively. Five samples contained at least one mutation associated with antiviral therapy resistance, and twenty-three samples contained at least one mutation associated with vaccine escape described in the literature. \u0000Conclusion. The present paper describes the stages of whole-genome sequencing of HBV, provides a laboratory protocol, nucleotide sequences of the primers and an approach to the data analysis. Using a list of clinical samples as example, the reliability of the panel is shown. The HBV panel holds immense potential for utilization in scientific research, epidemiological monitoring, and advancement of personalized medicine approaches.","PeriodicalId":86540,"journal":{"name":"Problems of virology","volume":"11 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140242068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Additional factors connected with the manifestation of the d-marker of poliomyelitis virus.","authors":"V I AGOL, M Y CHUMAKOVA","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":86540,"journal":{"name":"Problems of virology","volume":"6 ","pages":"672-4"},"PeriodicalIF":0.0,"publicationDate":"1961-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23618625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Virological and serological study of adenovirus conjunctivitis.","authors":"V O ANDZHELOV, R S DREIZIN","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":86540,"journal":{"name":"Problems of virology","volume":"6 ","pages":"509-13"},"PeriodicalIF":0.0,"publicationDate":"1961-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23622954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of a laboratory strain of trachoma virus pathogenic for man.","authors":"A A SHATKIN","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":86540,"journal":{"name":"Problems of virology","volume":"6 ","pages":"576-83"},"PeriodicalIF":0.0,"publicationDate":"1961-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23624027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E R PILLE, YEYA YERMAKOVA, Y U N ZUYEVA, L V NADAICHIK
{"title":"[Study of viruses isolated from monkeys].","authors":"E R PILLE, YEYA YERMAKOVA, Y U N ZUYEVA, L V NADAICHIK","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":86540,"journal":{"name":"Problems of virology","volume":"6 ","pages":"765-71"},"PeriodicalIF":0.0,"publicationDate":"1961-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23624383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V D SOLOV'EV, T G ORLOVA, L A PORUBEL, I N VASIL'EVA
{"title":"Study of the genetic characters of vaccine strains of type A-2 influenza virus.","authors":"V D SOLOV'EV, T G ORLOVA, L A PORUBEL, I N VASIL'EVA","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":86540,"journal":{"name":"Problems of virology","volume":"6 ","pages":"743-50"},"PeriodicalIF":0.0,"publicationDate":"1961-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23624878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A comparative study of the biological properties of strains of influenza virus isolated in Kiev in 1957 and 1959.","authors":"M F SMIRNOVA, L F SHEVCHENKO","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":86540,"journal":{"name":"Problems of virology","volume":"6 ","pages":"194-7"},"PeriodicalIF":0.0,"publicationDate":"1961-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23625245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}