Asia-pacific Journal of Molecular Biology and Biotechnology最新文献_第3页

Structural complexity and physical mechanism of self-assembled lipid as nanocarriers: A review 自组装脂质纳米载体的结构复杂性及物理机制研究进展

Asia-pacific Journal of Molecular Biology and Biotechnology Pub Date : 2023-04-06 DOI: 10.35118/apjmbb.2023.031.2.02

Nazhan Ilias, Rocky Vester Richmond, G. T. Selvarajah, I. D. Mat Azmi, M. Ajat

{"title":"Structural complexity and physical mechanism of self-assembled lipid as nanocarriers: A review","authors":"Nazhan Ilias, Rocky Vester Richmond, G. T. Selvarajah, I. D. Mat Azmi, M. Ajat","doi":"10.35118/apjmbb.2023.031.2.02","DOIUrl":"https://doi.org/10.35118/apjmbb.2023.031.2.02","url":null,"abstract":"Lipids such as glyceryl monooleate, phosphatidylcholine, and monoglyceride (CITREM) possess an amphipathic property that allows them to self-assemble into a complex internal structure when interacting with an aqueous solution. Since amphiphilic molecules possess hydrophilic heads and lipophilic tails, hydrophobic effects cause the spontaneous activity of the molecular rearrangement. The self-organization of the molecules often results in the phases of lipid polymorphism, for example microemulsion, inverse bicontinuous cubic (Q2), discontinuous hexagonal (H2), and micellar cubic (I2) Fd3m. Interestingly, these lamellar and non-lamellar phases have been applied in the development of nanocarriers for drug delivery due to their ability to provide a sustained drug release system, better drug bioavailability, and improved overall treatment. However, the attention that they are receiving from their application is not comparable to our understanding of the mechanisms involved in their synthesis. Elucidation of the spontaneous process helps in predicting and tuning the internal structure of an amphiphilic molecule to suit its application. Therefore, this review discusses the formation of lipid polymorphism from the thermodynamic point of view, critical packing parameter, and modified stalk theory.","PeriodicalId":8566,"journal":{"name":"Asia-pacific Journal of Molecular Biology and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91160458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Escherichia coli phages isolated from broiler chickens showed ideal characteristics in gut modulation 肉鸡大肠杆菌噬菌体具有理想的肠道调节特性

Asia-pacific Journal of Molecular Biology and Biotechnology Pub Date : 2023-04-05 DOI: 10.35118/apjmbb.2023.031.2.01

Mohd Asrore Mohd Shaufi, C. C. Sieo

{"title":"Escherichia coli phages isolated from broiler chickens showed ideal characteristics in gut modulation","authors":"Mohd Asrore Mohd Shaufi, C. C. Sieo","doi":"10.35118/apjmbb.2023.031.2.01","DOIUrl":"https://doi.org/10.35118/apjmbb.2023.031.2.01","url":null,"abstract":"Phage has gained interest as an alternative antibiotic growth promoter (AGPs) in poultry production. Most phage studies only focus on phages that target pathogens. In this study, we isolated and characterised phages that target non-pathogens from chicken intestines. This study aimed to isolate and characterise phages that target non-pathogenic Escherichia coli for gut modulation study in broiler chickens. Based on a morphological study, the C1 phage belonged to the Podoviridae family, whereas C2, C3, and C4 phages belonged to the Siphoviridae family. The C1, C2, C3, and C4 phages appeared to be unique based on restriction fragment length polymorphisms (RFLPs), amplification of phage signature genes, and protein profiling (SDS-PAGE). The C1 phage had an ideal multiplicity of infection (MOI) of 0.001, followed by 0.1 for the C2, C3, and C4 phages. C1 had the highest adsorption rate of 99.7% in 1 min, followed by C2 (98%), C3 (98.7%), and C4 (98.2%), all of which were within 2 min. C1 also exhibited the largest burst size (72 PFU/infected cell) and the shortest latent period (5 min). The latent period of the C2, C3, and C4 phages was longer, lasting 10 minutes, and their burst sizes were 70, 77, and 46 PFU/infected cells, respectively. All phages had optimum lytic activity at pH 7 and 37°C. Each phage was unique and possessed favourable lytic characteristics, making all of them suitable for gut modulation study in chickens.","PeriodicalId":8566,"journal":{"name":"Asia-pacific Journal of Molecular Biology and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74284658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of type IV pilin biosynthesis genes in biofilm formation of Aeromonas hydrophila IV型毛肽生物合成基因在嗜水气单胞菌生物膜形成中的作用

Asia-pacific Journal of Molecular Biology and Biotechnology Pub Date : 2023-03-06 DOI: 10.35118/apjmbb.2023.031.1.10

N. Garg, Geetika Sharma, D. Saffarini, S. Sharda, R. Sahney, Sheetal Shirodkar

引用次数: 0

The effect of HIV protease gene mutations to protease inhibitor drugs resistance in Papua patients: In silico analysis HIV蛋白酶基因突变对巴布亚患者蛋白酶抑制剂耐药性的影响:计算机分析

Asia-pacific Journal of Molecular Biology and Biotechnology Pub Date : 2023-03-01 DOI: 10.35118/apjmbb.2023.031.1.08

M. Widiyanti, Y. Maladan, S. Adiningsih

{"title":"The effect of HIV protease gene mutations to protease inhibitor drugs resistance in Papua patients: In silico analysis","authors":"M. Widiyanti, Y. Maladan, S. Adiningsih","doi":"10.35118/apjmbb.2023.031.1.08","DOIUrl":"https://doi.org/10.35118/apjmbb.2023.031.1.08","url":null,"abstract":"The use of phenotypic assay requires laborious work to culture HIV isolates to observe the phenotypic change of the virus in the presence of antiretroviral drugs. The genotypic approach may rely on the secondary data of documented mutations that are responsible for phenotypic alterations to antiretroviral-resistant HIV. HIV genomes were extracted from patients’ plasma, which was subsequently subjected to RT-PCR and Sanger sequencing. The obtained HIV genome sequencing data were analyzed for mutation detection. Three-dimensional (3D) structures of mutant HIV protease were constructed using FoldX software. The binding affinity of the mutant HIV protease with protease inhibitor drugs (Saquinavir, Ritonavir, Nelfinavir, Indinavir, and Lopinavir) was analyzed using AutoDock Vina. There were 90 patients involved in this study. The patients attended the Voluntary Counseling Test (VCT) of Mitra Masyarakat Hospital in Mimika, Papua, Indonesia. Among recruited subjects, the HIV genomes corresponding to the protease-encoded gene of 30 patients were successfully sequenced. There was only one patient (RSMM_70) infected with HIV harboring minor mutations (L10V, I15V, M36I, and R41K) in the protease-encoded gene that was not a new finding mutation. The 3D structure showed that the hydrophobicity and stability of mutant HIV protease were different from the wild genotype. Docking analysis showed decreasing binding affinity of the mutant HIV protease to the protease inhibitor drugs, which may lead to the alteration of inhibitory effectiveness. In silico docking, the analysis may provide an alternative approach to predict the effect of minor mutations in the HIV protease gene on the effectiveness of protease inhibitor drugs.","PeriodicalId":8566,"journal":{"name":"Asia-pacific Journal of Molecular Biology and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82917093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Invasive non-typhoidal Salmonella in adult patients: A three-year review in a Malaysian General Hospital 侵袭性非伤寒沙门氏菌在成年患者:三年回顾在马来西亚总医院

Asia-pacific Journal of Molecular Biology and Biotechnology Pub Date : 2023-03-01 DOI: 10.35118/apjmbb.2023.031.1.09

Karshini Jeya Pirathaba, Maria Kahar Bador Abdul Kahar, N. S. Che Hussin

{"title":"Invasive non-typhoidal Salmonella in adult patients: A three-year review in a Malaysian General Hospital","authors":"Karshini Jeya Pirathaba, Maria Kahar Bador Abdul Kahar, N. S. Che Hussin","doi":"10.35118/apjmbb.2023.031.1.09","DOIUrl":"https://doi.org/10.35118/apjmbb.2023.031.1.09","url":null,"abstract":"Invasive non-typhoidal Salmonella (iNTS) are recognized as an important cause of infection among immunocompromised patients with high morbidity and mortality. This study focused on the epidemiology, risk factors, antibiotic susceptibility, and clinical outcome of iNTS in Hospital Kuala Lumpur over 3 years period (2017-2019). Methods: A retrospective study was conducted on 43 adult patient who had NTS isolated from a sterile sample. The patient’s microbiology worksheets and medical notes were reviewed and analyzed. Results: The overall incidence rate for iNTS was 2.4 per 10,000 admissions. The patients median age was 57 years, with majority of them being above ≥ 55 years old (n=24, 55.8%). The most prevalent diseases are hypertension (28%), human immunodeficiency virus (20.9%), diabetes mellitus (18.6%) and heart disease (6.3%). Primary bacteraemia (95.3%) was the most frequent cause of iNTS, followed by tubo-ovarian abscess (2.3%) and urinary tract infections (2.3%). The overall mortality rate was 30%. The mortality rate was greater in patient presenting with shock on admission (p=0.04). Most of the invasive diseases were caused by Salmonella enterica serovar Enteritidis (95.3%). Overall, most isolates were susceptible to ceftriaxone (97.7%). Five isolates were intermediately resistant to ciprofloxacin (11.6%). Only one isolate (2.3%) was multidrug resistant. Conclusions: The comorbidities of iNTS varies even within the same country. Mortality rates are higher in those with primary bacteraemia and shock during admission. By understanding the epidemiology of iNTS in Malaysia, it will provide valuable information about the vulnerable population at risk of iNTS and improve the treatment and management of this patients.","PeriodicalId":8566,"journal":{"name":"Asia-pacific Journal of Molecular Biology and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75888177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of IL-28B serum level and gene polymorphism in a sample of Iraqi patients with ankylosing spondylitis

Asia-pacific Journal of Molecular Biology and Biotechnology Pub Date : 2023-02-27 DOI: 10.35118/apjmbb.2023.031.1.07

Hadeel Waleed Abdulmalek, Reema Mohammed Abed, Laith Ahmad Yaaqoob, M. F. Altaee, Z. K. Kamona

{"title":"Influence of IL-28B serum level and gene polymorphism in a sample of Iraqi patients with ankylosing spondylitis","authors":"Hadeel Waleed Abdulmalek, Reema Mohammed Abed, Laith Ahmad Yaaqoob, M. F. Altaee, Z. K. Kamona","doi":"10.35118/apjmbb.2023.031.1.07","DOIUrl":"https://doi.org/10.35118/apjmbb.2023.031.1.07","url":null,"abstract":"Ankylosing spondylitis (AS) represents one kind of advanced arthritis formed via inflammatory stimuli long-term in the spin‘s joints. Interleukin (IL)-29 (interferon- lambda1(IFN- λ1)), interleukin (IL)-28A (interferon- lambda 2 (IFN- λ2)) and interleukin (IL)-28B (interferon- lambda 3(IFN-λ3)) are three interferon lambda (IFN- λs) molecules that have recently been identified as new members of the IFN family. IL-28B expression in ankylosing spondylitis (AS) is not well understood. 150 male healthy controls ((HC) and 160 males with AS as patients group participated in this study. Serum level and gene polymorphism were assessed using an enzyme-linked immunosorbent assay and Sanger sequencing for IL-28B, respectively. The results showed significantly lower serum IL-28B concentrations in the AS groups in comparison to the HC groups (both p values equal to 0.003). There was a large difference in IL-28B genotype and allele frequency between the two individuals. IL-28B heterozygote genotype CT of rs12979860 SNP exhibits a substantial correlation with AS (P = 0.008). While the genotypes of rs12980275 SNP were not shown any significant correlation with AS. The findings suggest that serum concentration of IL-28B is a potential diagnostic biomarker in patients with AS, and that the heterozygote CT of rs12979860 SNP serves as a potential risk factor for the onset of AS in the Iraqi population.","PeriodicalId":8566,"journal":{"name":"Asia-pacific Journal of Molecular Biology and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73645159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of endophytic bacteria colonisation in tissue culture-derived oil palm plantlets via green fluorescent visualization 通过绿色荧光可视化验证组织培养油棕植株内生细菌定植

Asia-pacific Journal of Molecular Biology and Biotechnology Pub Date : 2023-02-24 DOI: 10.35118/apjmbb.2023.031.1.06

Salwa Abdullah Sirajuddin, Intan Nur Ainni Mohamed Azni, N. Roslan, S. Sundram

{"title":"Validation of endophytic bacteria colonisation in tissue culture-derived oil palm plantlets via green fluorescent visualization","authors":"Salwa Abdullah Sirajuddin, Intan Nur Ainni Mohamed Azni, N. Roslan, S. Sundram","doi":"10.35118/apjmbb.2023.031.1.06","DOIUrl":"https://doi.org/10.35118/apjmbb.2023.031.1.06","url":null,"abstract":"This study aimed to validate the colonisation capability of endophytic bacteria (EB) isolates, Bacillus cereus EB2 and Pseudomonas aeruginosa EB35, that previously exhibited their potentials as biological control agents (BCAs) against the Ganoderma spp., a pathogen for Ganoderma disease in oil palm. Here, we demonstrated a rapid method to determine the colonisation capacity of the selected EB using oil palm tissue culture plantlets and a green fluorescent protein (GFP) visual marker. Wounded plantlet roots were inoculated with GFP-tagged B. cereus EB2 and P. aeruginosa EB35 while the plantlets without EB inoculation served as controls. The GFP signals appeared as bright green spots or lines in the inoculated GFP-tagged EB cells in root and leaf plantlet tissues, respectively, under the confocal laser scanning microscopy (CLSM) 5 days post-inoculation. In contrast, there was no intense GFP spots in neither the control root nor leaf tissues. The cracks in the roots by wounding facilitated the entry of the GFP-tagged EB cells into root tissues, allowing for endophytically colonisation of the root and above-ground tissues. Subsequent result of polymerase chain reaction (PCR)-GFP analysis further displayed the endophytic nature and early chronological colonisation of the tested EB. This is a preliminary report on root colonisation by a Gram-positive endophyte, B. cereus EB2 and leaf tissues colonisation by both EB isolates as internal colonisers, demonstrating their potential as BCAs to protect oil palm against Ganoderma spp. for a sustainable disease management.","PeriodicalId":8566,"journal":{"name":"Asia-pacific Journal of Molecular Biology and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85302098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple approach for expression and rapid purification of Taq DNA polymerase in three Escherichia coli strains 三株大肠杆菌中Taq DNA聚合酶的简单表达和快速纯化方法

Asia-pacific Journal of Molecular Biology and Biotechnology Pub Date : 2023-02-19 DOI: 10.35118/apjmbb.2023.031.1.05

Xue Chi Teng, See Yin Ang, Marimuthu Citartan, T. Tang, Siti Aminah Ahmed

{"title":"Simple approach for expression and rapid purification of Taq DNA polymerase in three Escherichia coli strains","authors":"Xue Chi Teng, See Yin Ang, Marimuthu Citartan, T. Tang, Siti Aminah Ahmed","doi":"10.35118/apjmbb.2023.031.1.05","DOIUrl":"https://doi.org/10.35118/apjmbb.2023.031.1.05","url":null,"abstract":"Background and objectives: One of the most commonly employed bacterial hosts for the synthesis of recombinant proteins is Escherichia coli (E. coli). Choosing an ideal host for the production of the protein of interest is an important step in the large-scale production process. Due to its thermostable characteristic, Taq Pol I, which was first isolated from the bacterium Thermus aquaticus (Taq), is now a typical enzyme found in many laboratories. This study aimed to identify the ideal host for large-scale production of Taq Pol I and purify the enzyme under a simple and rapid purification method. Methods: Taq Pol I gene in pSE420 plasmid was overexpressed in E. coli strains DH5α, TOP10 and BL21(DE3) pLysS. The enzyme was purified using Pluthero’s method and dialyzed using Amicon® Ultra-4 Centrifugal Filter. Results: The host strain E. coli TOP10 produced the highest amounts of Taq Pol I, followed by DH5α and BL21(DE3) pLysS. An estimated 4.5 U/μL of Taq Pol I was produced from a 200 mL culture of TOP10 host. Conclusion: This study provides data on the capacity of the E. coli strains used for Taq Pol I protein production. This information can be used to accelerate future targeted strain selection for the production of a specific protein of interest.","PeriodicalId":8566,"journal":{"name":"Asia-pacific Journal of Molecular Biology and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88495570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Primer characterization of in-house real time PCR (RT-PCR) for BCL2 gene using saliva sample 利用唾液样本进行BCL2基因内部实时PCR (RT-PCR)引物鉴定

Asia-pacific Journal of Molecular Biology and Biotechnology Pub Date : 2023-02-19 DOI: 10.35118/apjmbb.2023.031.1.04

Indra Wahyu Nufroha, Adri Nora, H. Saraswati

{"title":"Primer characterization of in-house real time PCR (RT-PCR) for BCL2 gene using saliva sample","authors":"Indra Wahyu Nufroha, Adri Nora, H. Saraswati","doi":"10.35118/apjmbb.2023.031.1.04","DOIUrl":"https://doi.org/10.35118/apjmbb.2023.031.1.04","url":null,"abstract":"In organisms, cells perform apoptosis to remove damaged or mutated cells from the body. The Bcl-2 family protein encoded by the BCL2 gene plays a role in regulating apoptosis. Abnormalities in the function and expression level of the Bcl-2 protein are associated with several cancers. Saliva is a body fluid that can be used for biomedical research because it contains essential biomarkers of the body and genetic material derived from free cells in the oral cavity. This study aims to get primer characterization for the RT-PCR method for the BCL2 gene. We used RNA samples isolated from saliva to optimize the primers' annealing temperature, concentration, and combination pairs. Previous studies produced three primer candidates, i.e., primers A, B, and C, used in this research. The optimization results showed that primer C was the best primer to be used in the real-time PCR of this study. The optimal annealing temperature used was 60.3°C with a primer concentration of 400 nM. This study also shows the potential of saliva as a material for biomedical studies on the BCL2 gene. The results of the primer characterization resulting from this research are the first step in establishing the in-house RT-PCR method. The validation research will use a larger sample to validate this method.","PeriodicalId":8566,"journal":{"name":"Asia-pacific Journal of Molecular Biology and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72429912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of capsid polypeptide P1 and capsid protein VP1 of the Malaysia foot and mouth disease virus (FMDV) serotype O and A isolates 马来西亚口蹄疫病毒(FMDV)血清O型和A型分离株衣壳多肽P1和衣壳蛋白VP1的鉴定

Asia-pacific Journal of Molecular Biology and Biotechnology Pub Date : 2023-02-10 DOI: 10.35118/apjmbb.2023.031.1.03

Farah Najwa Abd-Halin, Z. Zakaria, S. Ismail, S. Othman

{"title":"Characterisation of capsid polypeptide P1 and capsid protein VP1 of the Malaysia foot and mouth disease virus (FMDV) serotype O and A isolates","authors":"Farah Najwa Abd-Halin, Z. Zakaria, S. Ismail, S. Othman","doi":"10.35118/apjmbb.2023.031.1.03","DOIUrl":"https://doi.org/10.35118/apjmbb.2023.031.1.03","url":null,"abstract":"Foot and mouth disease virus (FMDV) is the cause of foot and mouth disease (FMD) outbreaks in livestock worldwide, which affects domestic and international trade, resulting in significant economic losses and social consequences. For efficient monitoring and prevention of FMD outbreaks, the need for improved strategies to control FMDV and achieve FMD-free status with various control measures including vaccination can be established. In vaccinology, major advances and discoveries in vaccination variations including DNA and protein subunit vaccines proved to be more economical and sustainable. To develop a safe vaccine for animals, possible antigenic genes or antigens need to be identified and characterised. The FMDV is a single-stranded RNA virus consisting of a capsid precursor polypeptide, P1, which encodes for four structural proteins (VP4-1), leading to antigenic variation and VP1 potentially carrying the key epitope for vaccine development. This study aims to identify and characterise the capsid polypeptide, P1 and capsid protein, VP1 of the Malaysian FMDV serotype O and serotype A isolates. The nucleotide and protein sequences were identified based on the FMD outbreaks in Malaysia and the antigenicity of the P1 and VP1 was predicted by Kolaskar and Tongaonkar's semi-empirical method. Subsequently, the P1 and VP1 genes were inserted into pET-28a, respectively, and used for protein expression analysis. The P1 and VP1 were predicted to be antigenic via in silico analysis and successfully expressed and characterised through in vitro analysis. Hence, this study can be exploited as a tool to design a new novel vaccine for vaccine development against FMD in bovines.","PeriodicalId":8566,"journal":{"name":"Asia-pacific Journal of Molecular Biology and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88654131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0