Asian Pacific journal of tropical biomedicine最新文献

Oleanolic acid inhibits mitochondrial fission by improving mitochondrial dysfunction and reducing atherosclerosis via regulating AMPK/Drp1 pathway: An in vivo and in vitro study 齐墩果酸通过调节AMPK/Drp1通路改善线粒体功能障碍，减少动脉粥样硬化，从而抑制线粒体裂变：体内和体外研究

4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2025-12-28 DOI: 10.4103/apjtb.apjtb_511_25

Jin-zhou Xie, Wei-Jia Cui, Wei-Tian Zhong, Liang Ning

{"title":"Oleanolic acid inhibits mitochondrial fission by improving mitochondrial dysfunction and reducing atherosclerosis via regulating AMPK/Drp1 pathway: An in vivo and in vitro study","authors":"Jin-zhou Xie, Wei-Jia Cui, Wei-Tian Zhong, Liang Ning","doi":"10.4103/apjtb.apjtb_511_25","DOIUrl":"https://doi.org/10.4103/apjtb.apjtb_511_25","url":null,"abstract":"Objective: To investigate effect of oleanolic acid (OA) on atherosclerosis and its related mechanisms. Methods: Human umbilical vein endothelial cells (HUVECs) were injured by oxidized low-density lipoprotein for 24 h and treated with OA, and the levels of cell proliferation, migration, adhesion, and apoptosis were evaluated by BrdU staining, scratch healing assay, monocyte-endothelial cell adhesion assay and flow cytometry. The mice were fed with a high-fat diet to induce an atherosclerosis model, and treated with OA by gastric gavage. The mice were divided into the control group, the model group, and the OA administration group. The blood lipid and plaque formation in mice were detected. In addition, oxidative stress and mitochondrial structure and function changes in cells and mice were evaluated by transmission electron microscopy, JC-1 fluorescent probe, and Western blotting assays. The expression levels of proteins in the AMPK/Drp1 pathway were examined through Western blot. Results: OA markedly increased cell viability and migration rate of HUVECs, and decreased the adhesion rate of THP-1 cells and the apoptosis rate. OA significantly reduced serum lipid levels, such as total cholesterol and triglyceride, in mice and inhibited plaque formation in the aorta. OA also significantly increased the content of superoxide dismutase and catalase, alleviated mitochondrial damage, such as mitochondrial swelling and mitochondrial cristae reduction, reduced the number of mitochondria, increased adenosine triphosphate content, and significantly reduced p-Drp1 (Ser616)/Drp1, MFF and FIS1 levels, increased p-AMPK/AMPK levels, activated AMPK, and then regulated DRP1 activity. Conclusions: OA activates AMPK, which in turn regulates the activity of DRP1 to restore normal mitochondrial dynamics and reduce atherosclerosis.","PeriodicalId":8560,"journal":{"name":"Asian Pacific journal of tropical biomedicine","volume":"16 1","pages":"24-39"},"PeriodicalIF":0.0,"publicationDate":"2025-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alpiniae oxyphyllae Fructus ameliorates renal lipid accumulation in diabetic kidney disease via activating PPARα 木叶连翘通过激活PPARα改善糖尿病肾病的肾脂质积累

4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2025-01-01 DOI: 10.4103/apjtb.apjtb_703_24

Zijie Yan, Lin Zhang, Xingmin Han, Yu Kang, Shuman Liu, Tianpeng Ma, Man Xiao, Yiqiang Xie

{"title":"Alpiniae oxyphyllae Fructus ameliorates renal lipid accumulation in diabetic kidney disease via activating PPARα","authors":"Zijie Yan, Lin Zhang, Xingmin Han, Yu Kang, Shuman Liu, Tianpeng Ma, Man Xiao, Yiqiang Xie","doi":"10.4103/apjtb.apjtb_703_24","DOIUrl":"https://doi.org/10.4103/apjtb.apjtb_703_24","url":null,"abstract":"Objective: To investigate the effects of Alpiniae oxyphyllae Fructus (AOF) on renal lipid deposition in diabetic kidney disease (DKD) and elucidate its molecular mechanisms. Methods: The mechanism of AOF in treating DKD was explored by network pharmacological enrichment analysis, molecular docking, and molecular dynamics simulation. The effects of AOF on renal function and lipid deposition were assessed in a mouse model of DKD and high glucose-stressed HK-2 cells. Cell viability and lipid accumulation were detected by CCK8 and oil red O staining. The expressions of PPARα and fatty acid oxidation-related genes ( ACOX1 and CPT1A ) were detected by quantitative RT-PCR, Western blot, and immunofluorescence. Furthermore, PPARα knockdown was performed to examine the molecular mechanism of AOF in treating DKD. Results: Network pharmacological enrichment analysis, molecular docking, and molecular dynamics simulation showed that the active compounds in AOF targeted PPARα and thus transcriptionally regulated ACOX1 and CPT1A. AOF lowered blood glucose, improved dyslipidemia, and attenuated renal injury in DKD mice. AOF-containing serum accentuated high glucose-induced decrease in cell viability and ameliorated lipid accumulation. Additionally, it significantly upregulated the expression of PPARα, ACOX1, and CPT1A in both in vivo and in vitro experiments, which was reversed by PPARα knockdown. Conclusions: AOF may promote fatty acid oxidation via PPARα to ameliorate renal lipid deposition in DKD.","PeriodicalId":8560,"journal":{"name":"Asian Pacific journal of tropical biomedicine","volume":"15 1","pages":"11-23"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Recent advances on pathogenesis, diagnosis, prevention, immunological aspects, and vectors of dengue: A review 登革热的发病机制、诊断、预防、免疫学和媒介研究进展综述

IF 1.7 4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2023-08-01 DOI: 10.4103/2221-1691.383687

MaheshChandra Sahu, RakeshKumar Samantaray, Aditi Pal, Sanghamitra Pati

引用次数: 0

Hydroxysafflor yellow A protects against thioacetamide-induced liver fibrosis in rats via suppressing proinflammatory/fibrogenic mediators and promoting hepatic stellate cell senescence and apoptosis 羟基红花黄色素A通过抑制促炎/纤维化介质和促进肝星状细胞衰老和凋亡来对抗硫代乙酰胺诱导的大鼠肝纤维化

IF 1.7 4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2023-08-01 DOI: 10.4103/2221-1691.383689

SayedH. Seif el-Din, OlfatA. Hammam, ShahiraM. Ezzat, Samira Saleh, MarwaM. Safar, WalaaH. El-Maadawy, NaglaaM. El-Lakkany

引用次数: 0

Phellinus igniarius ameliorates renal aging in a rat model of focal and segmental glomerulosclerosis 在局灶性和节段性肾小球硬化大鼠模型中，白藜芦醇可改善肾脏老化

IF 1.7 4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2023-08-01 DOI: 10.4103/2221-1691.383690

Ru-Chun Yang, Zhou-Ting Wang, Yue-Wen Tang, Feng Wan, Yan Guo, Jie Zheng, You-Gui Li, Wei-Ming He

引用次数: 0

Aqueous extract of Protaetia brevitarsis larvae increases mTOR-mediated growth rate in zebrafish larvae 短尾原毛滴虫幼虫的水提取物提高斑马鱼幼虫mTOR介导的生长速率

IF 1.7 4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2023-08-01 DOI: 10.4103/2221-1691.383688

Gi-Young Kim, J. Jayasingha, KyoungTae Lee, YungHyun Choi

引用次数: 0

Characterization and antimicrobial, antioxidant, and anti-proliferative activities of green synthesized magnesium oxide nanoparticles with shoot extracts of Plicosepalus curviflorus 弯曲珊瑚茎提取物绿色合成氧化镁纳米颗粒的表征及其抗菌、抗氧化和抗增殖活性

IF 1.7 4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2023-07-01 DOI: 10.4103/2221-1691.380563

Reem Alrashoudi, Manal Abudawood, Ayesha Mateen, H. Tabassum, Noura Alghumlas, S. Fatima, Basmah Almaarik, Farah Maqsood, N. A. Al Musayeib, M. Amina

{"title":"Characterization and antimicrobial, antioxidant, and anti-proliferative activities of green synthesized magnesium oxide nanoparticles with shoot extracts of Plicosepalus curviflorus","authors":"Reem Alrashoudi, Manal Abudawood, Ayesha Mateen, H. Tabassum, Noura Alghumlas, S. Fatima, Basmah Almaarik, Farah Maqsood, N. A. Al Musayeib, M. Amina","doi":"10.4103/2221-1691.380563","DOIUrl":"https://doi.org/10.4103/2221-1691.380563","url":null,"abstract":"Objective: To synthesize magnesium oxide nanoparticles using ethanol extract of shoots of Plicosepalus curviflorus (PC-MgONPs) and evaluate the antimicrobial, antioxidant, and anti-proliferative activities of PC-MgONPs. Methods: The green synthesized PC-MgONPs were characterized by ultraviolet-visible (UV), Fourier-transform infrared spectroscopy, zeta potential, energy dispersive X-ray, and scanning electron microscopy. Furthermore, we investigated total antioxidant capacity and antimicrobial and anti-proliferative activities using breast cancer cell lines (MDA-231). Results: The UV spectrum of PC-MgONPs showed a sharp absorption peak at 300 nm. The presence of magnesium, oxygen, and sodium was confirmed by energy dispersive X-ray analysis. Scanning electron microscopy revealed PC-MgONPs as roughly spherical granular structures with sizes ranging from 20.0 to 76.4 nm. PC-MgONPs showed considerable antimicrobial activities against Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans with zones of inhibition of 11-17 mm. In addition, total antioxidant capacity and anti-proliferative activity of PC-MgONPs against MDA-231 cells were dose-dependent. Conclusions: The synthesized PC-MgONPs could be a potent antimicrobial, antioxidant and anti-cancer agent, which needs further investigation.","PeriodicalId":8560,"journal":{"name":"Asian Pacific journal of tropical biomedicine","volume":"13 1","pages":"315 - 324"},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48697326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myricetin alleviates ovalbumin-induced allergic rhinitis in mice by regulating Th1/Th2 balance 杨梅素通过调节Th1/Th2平衡减轻卵清蛋白诱导的小鼠变应性鼻炎

IF 1.7 4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2023-07-01 DOI: 10.4103/2221-1691.380562

J. Geng, Yanran Shi, Jinsong Su, Juan Wang

{"title":"Myricetin alleviates ovalbumin-induced allergic rhinitis in mice by regulating Th1/Th2 balance","authors":"J. Geng, Yanran Shi, Jinsong Su, Juan Wang","doi":"10.4103/2221-1691.380562","DOIUrl":"https://doi.org/10.4103/2221-1691.380562","url":null,"abstract":"Objective: To evaluate the effect of myricetin on ovalbumin (OVA)-induced allergic rhinitis in mice. Methods: Mice were sensitized and challenged using OVA (5%, 500 mL) intraperitoneally and intranasally, respectively, on an alternative day for 14 days, followed by administration of myricetin (50, 100, and 200 mg/kg) till day 21. Nasal symptoms, biochemical parameters, protein expressions, and histopathology were observed. Results: OVA-induced increased nasal symptoms including rubbing, sneezing, and discharge were significantly reduced by myricetin (100 and 200 mg/kg) (P<0.05). Myricetin also protected against histamine challenge and attenuated elevated serum immunoglobulin E (IgE; total and OVA-specific), total IgG1, and β-hexosaminidase levels, as well as leukotriene C4 and interleukins levels in nasal lavage fluid (P<0.05). Western blot analysis showed that myricetin significantly upregulated the protein expression of T-box expressed in T cells, while downregulating the protein expression of GATA binding protein 3, NF-κB, and 1κВ-α (P<0.05). Additionally, OVA-induced histopathological abberations in the nasal mucosa was markedly ameliorated by myricetin treatment (P<0.05). Conclusions: Myricetin exerts anti-allergic effects against OVA-induced allergic rhinitis via regulating Th1/Th2 balance.","PeriodicalId":8560,"journal":{"name":"Asian Pacific journal of tropical biomedicine","volume":"13 1","pages":"306 - 314"},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43167339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Role of flaxseed (Linum usitatissimum L.) in disease prevention and treatment 亚麻籽在疾病防治中的作用

IF 1.7 4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2023-07-01 DOI: 10.4103/2221-1691.380559

A. Zanwar, C. Manimurugan, M. Sujatha, A. Rathnakumar, M. Sandhanalakshmi

引用次数: 0

Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats 表没食子儿茶素-3-没食子酸盐对自发性高血压大鼠的降压作用及改善内皮功能

IF 1.7 4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2023-07-01 DOI: 10.4103/2221-1691.380560

Yucinda Khor, Siew-Keah Lee, M. Dharmani Devi, W. Ling

{"title":"Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats","authors":"Yucinda Khor, Siew-Keah Lee, M. Dharmani Devi, W. Ling","doi":"10.4103/2221-1691.380560","DOIUrl":"https://doi.org/10.4103/2221-1691.380560","url":null,"abstract":"Objective: To investigate the effect of epigallocatechin-3-gallate (EGCG) on endothelial dysfunction in spontaneously hypertensive rats (SHR). Methods: Wistar-Kyoto (WKY) rats and SHR were divided into four groups; WKY control, SHR control and SHR treated with EGCG (50 mg/kg/day) or losartan (10 mg/kg/day). The treatment was given daily for 4 weeks by oral gavage and the blood pressure was monitored by tail-cuff method every 3 days. Acetylcholine-induced endothelium-dependent relaxations were assessed in isolated phenylephrine-precontracted aortic rings at the end of treatment. The vascular levels of reactive oxygen species, nitric oxide, tetrahydrobiopterin, and cyclic guanosine monophosphate were also measured. Moreover, the expression of angiotensin II type 1 (AT1) receptor protein was determined. Results: The systolic blood pressure was significantly decreased in SHR treated with EGCG. The impaired endothelium-dependent relaxation was significantly improved in aortic ring isolated from the EGCG-treated SHR group. EGCG also significantly increased the levels of nitric oxide, tetrahydrobiopterin, and cyclic guanosine monophosphate, while decreasing the level of reactive oxygen species and the protein expression of AT1 receptor in SHR. Conclusions: EGCG attenuates endothelial dysfunction in SHR by decreasing oxidative stress and increasing vascular nitric oxide bioavailability, which may be modulated partly by inhibition of vascular AT1 receptors. An increase in endothelium-dependent relaxation may contribute to a decrease in blood pressure in hypertensive animals.","PeriodicalId":8560,"journal":{"name":"Asian Pacific journal of tropical biomedicine","volume":"13 1","pages":"287 - 295"},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43789664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1