Asian Pacific journal of tropical biomedicine最新文献

Curcumin attenuates pressure overload-induced cardiac fibrosis in mice via modulating the miR-29b/HDAC4 axis 姜黄素通过调节miR-29b/HDAC4轴减轻小鼠压力过载诱导的心脏纤维化

4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2026-01-15 DOI: 10.4103/apjtb.apjtb_622_25

Yu Xiang, Ying Lv, Xiaoxiang Liu, Botao Li, Jing Liu

{"title":"Curcumin attenuates pressure overload-induced cardiac fibrosis in mice via modulating the miR-29b/HDAC4 axis","authors":"Yu Xiang, Ying Lv, Xiaoxiang Liu, Botao Li, Jing Liu","doi":"10.4103/apjtb.apjtb_622_25","DOIUrl":"https://doi.org/10.4103/apjtb.apjtb_622_25","url":null,"abstract":"Objective: To investigate the antifibrotic effects of curcumin in a transverse aortic constriction (TAC) mouse model and elucidate its molecular mechanisms. Methods: Male C57BL/6 mice underwent TAC and received vehicle, low-dose curcumin (50 mg/kg), high-dose curcumin (200 mg/kg), high-dose curcumin plus a scrambled control antagomir, or high-dose curcumin plus anti-miR-29b treatments. Cardiac function was assessed by echocardiography. Fibrosis was evaluated by histology, collagen volume fraction, and hydroxyproline content. Expression of miR-29b, HDAC4, and fibrosis-related markers (Collai, Col3a1, TGF-β1) was measured by quantitative RT-PCR and Western blotting assays. Myocardial procollagen type I carboxyterminal propeptide was determined by ELISA, and HDAC4-specific enzymatic activity was assayed using a fluorogenic kit. Results: Curcumin improved cardiac function, reduced fibrosis, restored miR-29b expression, and suppressed HDAC4 expression and activity in a dose-dependent manner. Furthermore, curcumin decreased myocardial procollagen type I carboxy-terminal propeptide levels, confirming reduced collagen synthesis. Anti-miR-29b administration partially abrogated the antifibrotic and cardioprotective effects of curcumin. Conclusions: Curcumin attenuates pressure overload-induced cardiac fibrosis and dysfunction in a TAC mouse model via modulation of the miR-29b/HDAC4 axis and suppression of collagen synthesis.","PeriodicalId":8560,"journal":{"name":"Asian Pacific journal of tropical biomedicine","volume":"16 2","pages":"59-67"},"PeriodicalIF":0.0,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147892568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oleanolic acid inhibits mitochondrial fission by improving mitochondrial dysfunction and reducing atherosclerosis via regulating AMPK/Drp1 pathway: An in vivo and in vitro study 齐墩果酸通过调节AMPK/Drp1通路改善线粒体功能障碍，减少动脉粥样硬化，从而抑制线粒体裂变：体内和体外研究

4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2025-12-28 DOI: 10.4103/apjtb.apjtb_511_25

Jin-zhou Xie, Wei-Jia Cui, Wei-Tian Zhong, Liang Ning

{"title":"Oleanolic acid inhibits mitochondrial fission by improving mitochondrial dysfunction and reducing atherosclerosis via regulating AMPK/Drp1 pathway: An in vivo and in vitro study","authors":"Jin-zhou Xie, Wei-Jia Cui, Wei-Tian Zhong, Liang Ning","doi":"10.4103/apjtb.apjtb_511_25","DOIUrl":"https://doi.org/10.4103/apjtb.apjtb_511_25","url":null,"abstract":"Objective: To investigate effect of oleanolic acid (OA) on atherosclerosis and its related mechanisms. Methods: Human umbilical vein endothelial cells (HUVECs) were injured by oxidized low-density lipoprotein for 24 h and treated with OA, and the levels of cell proliferation, migration, adhesion, and apoptosis were evaluated by BrdU staining, scratch healing assay, monocyte-endothelial cell adhesion assay and flow cytometry. The mice were fed with a high-fat diet to induce an atherosclerosis model, and treated with OA by gastric gavage. The mice were divided into the control group, the model group, and the OA administration group. The blood lipid and plaque formation in mice were detected. In addition, oxidative stress and mitochondrial structure and function changes in cells and mice were evaluated by transmission electron microscopy, JC-1 fluorescent probe, and Western blotting assays. The expression levels of proteins in the AMPK/Drp1 pathway were examined through Western blot. Results: OA markedly increased cell viability and migration rate of HUVECs, and decreased the adhesion rate of THP-1 cells and the apoptosis rate. OA significantly reduced serum lipid levels, such as total cholesterol and triglyceride, in mice and inhibited plaque formation in the aorta. OA also significantly increased the content of superoxide dismutase and catalase, alleviated mitochondrial damage, such as mitochondrial swelling and mitochondrial cristae reduction, reduced the number of mitochondria, increased adenosine triphosphate content, and significantly reduced p-Drp1 (Ser616)/Drp1, MFF and FIS1 levels, increased p-AMPK/AMPK levels, activated AMPK, and then regulated DRP1 activity. Conclusions: OA activates AMPK, which in turn regulates the activity of DRP1 to restore normal mitochondrial dynamics and reduce atherosclerosis.","PeriodicalId":8560,"journal":{"name":"Asian Pacific journal of tropical biomedicine","volume":"16 1","pages":"24-39"},"PeriodicalIF":0.0,"publicationDate":"2025-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial properties of Sonchus species: A review Sonchus属植物抗菌特性研究进展

4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2025-05-01 DOI: 10.4103/apjtb.apjtb_42_25

Nurin Naziha Zulkefle, Nurhafiza Zainal, Dwi Kusuma Wahyuni, Syed Esam Mahmood, Kivaandra Dayaa Rao Ramarao, Kim Ling Chin

{"title":"Antimicrobial properties of Sonchus species: A review","authors":"Nurin Naziha Zulkefle, Nurhafiza Zainal, Dwi Kusuma Wahyuni, Syed Esam Mahmood, Kivaandra Dayaa Rao Ramarao, Kim Ling Chin","doi":"10.4103/apjtb.apjtb_42_25","DOIUrl":"https://doi.org/10.4103/apjtb.apjtb_42_25","url":null,"abstract":"As a rich source of bioactive compounds, plant-derived natural products hold immense promise for pharmaceutical research and development, offering a path to discovering new drug targets and innovative treatments. Sonchus species, commonly known as sow thistles, are widely distributed across various geographical regions. Several common species within this plant group have a long history of use in traditional medicine, which can be attributed to their diverse therapeutic benefits. Recently, these plants have gained attention for their potential in antimicrobial therapy, owing to their rich phytochemical profile, including sesquiterpene, flavonoids, and phenolic acids with known antimicrobial activities. Currently, available pharmacological studies demonstrate that Sonchus species extracts possess antimicrobial properties, highlighting their potential as sources for new therapeutic agents. This review provides an overview of the current knowledge on the antibacterial, antifungal, antiparasitic, and antiviral activities of the Sonchus species, along with its possible mechanisms of action. The review aims to provide directions for future research and promote the exploration of these plants as viable sources of natural antimicrobial agents, potentially contributing to the development of alternative therapeutic strategies in the face of rising antibiotic resistance.","PeriodicalId":8560,"journal":{"name":"Asian Pacific journal of tropical biomedicine","volume":"15 5","pages":"177-188"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147885430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Alpiniae oxyphyllae Fructus ameliorates renal lipid accumulation in diabetic kidney disease via activating PPARα 木叶连翘通过激活PPARα改善糖尿病肾病的肾脂质积累

4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2025-01-01 DOI: 10.4103/apjtb.apjtb_703_24

Zijie Yan, Lin Zhang, Xingmin Han, Yu Kang, Shuman Liu, Tianpeng Ma, Man Xiao, Yiqiang Xie

{"title":"Alpiniae oxyphyllae Fructus ameliorates renal lipid accumulation in diabetic kidney disease via activating PPARα","authors":"Zijie Yan, Lin Zhang, Xingmin Han, Yu Kang, Shuman Liu, Tianpeng Ma, Man Xiao, Yiqiang Xie","doi":"10.4103/apjtb.apjtb_703_24","DOIUrl":"https://doi.org/10.4103/apjtb.apjtb_703_24","url":null,"abstract":"Objective: To investigate the effects of Alpiniae oxyphyllae Fructus (AOF) on renal lipid deposition in diabetic kidney disease (DKD) and elucidate its molecular mechanisms. Methods: The mechanism of AOF in treating DKD was explored by network pharmacological enrichment analysis, molecular docking, and molecular dynamics simulation. The effects of AOF on renal function and lipid deposition were assessed in a mouse model of DKD and high glucose-stressed HK-2 cells. Cell viability and lipid accumulation were detected by CCK8 and oil red O staining. The expressions of PPARα and fatty acid oxidation-related genes ( ACOX1 and CPT1A ) were detected by quantitative RT-PCR, Western blot, and immunofluorescence. Furthermore, PPARα knockdown was performed to examine the molecular mechanism of AOF in treating DKD. Results: Network pharmacological enrichment analysis, molecular docking, and molecular dynamics simulation showed that the active compounds in AOF targeted PPARα and thus transcriptionally regulated ACOX1 and CPT1A. AOF lowered blood glucose, improved dyslipidemia, and attenuated renal injury in DKD mice. AOF-containing serum accentuated high glucose-induced decrease in cell viability and ameliorated lipid accumulation. Additionally, it significantly upregulated the expression of PPARα, ACOX1, and CPT1A in both in vivo and in vitro experiments, which was reversed by PPARα knockdown. Conclusions: AOF may promote fatty acid oxidation via PPARα to ameliorate renal lipid deposition in DKD.","PeriodicalId":8560,"journal":{"name":"Asian Pacific journal of tropical biomedicine","volume":"15 1","pages":"11-23"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antioxidant, antimicrobial, and anticancer effects of Achillea filipendulina L. against colon cancer 水蛭对结肠癌的抗氧化、抗菌和抗癌作用

4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2024-12-01 DOI: 10.4103/apjtb.apjtb_515_24

Tutku Tunç, Şeyda Akın, Okan Aykaç, Ceylan Hepokur, Serap Duran, Hülya Özpınar

{"title":"Antioxidant, antimicrobial, and anticancer effects of Achillea filipendulina L. against colon cancer","authors":"Tutku Tunç, Şeyda Akın, Okan Aykaç, Ceylan Hepokur, Serap Duran, Hülya Özpınar","doi":"10.4103/apjtb.apjtb_515_24","DOIUrl":"https://doi.org/10.4103/apjtb.apjtb_515_24","url":null,"abstract":"Objective: To investigate the antioxidant, antimicrobial, and anticancer effects of Achillea filipendulina . Methods: Phytochemical content was analyzed by GC-MS. Antioxidant activity was assayed by DPPH, total antioxidant status, total oxidant status, and iron reduction tests. Antimicrobial activity was assessed using the MIC assay. Anticancer activity was detected via MTT assay, followed by analysis of the apoptotic process using annexin V and caspase 3/7. miR-519d and Dvl-1 expression were assessed by RT-PCR, and molecular docking analysis was also performed. Results: The ethanol extract of Achillea filipendulina showed the strongest anticancer effect. A significant increase in miR-519d expression was observed in HT29 cells, while a decrease in Dvl-1 expression was noted. DPPH, FRAP, total antioxidant status, and total oxidant assays showed high antioxidant activity. The hexane and ethanol extracts were effective against Streptococcus mutans (MIC<50 µg/mL), while the ethanol extract was effective against Enterococcus faecalis and Candida albicans (MIC<50 µg/mL). Molecular docking studies also confirmed that ethanol extracts could act on the Wnt pathway. GC-MS analysis showed high level of eucalyptol and borneol content in the extract. Conclusions: Ethanol extract of Achillea filipendulina has anticancer effect and can be used in colon cancer treatments.","PeriodicalId":8560,"journal":{"name":"Asian Pacific journal of tropical biomedicine","volume":"14 12","pages":"540-550"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147885775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Thrombopoietin ameliorates doxorubicin-induced toxicities in H9c2 myocardiocytes by inhibiting oxidative stress through the SIRT1/p38 MAPK signaling pathway 血小板生成素通过SIRT1/p38 MAPK信号通路抑制氧化应激，改善阿霉素诱导的H9c2心肌细胞毒性

4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2024-09-01 DOI: 10.4103/apjtb.apjtb_346_24

Xu-Han Zuo, Yu Huang, Bo-Cen Chen, Mingyue Zhu, Caicai Zhang, Han-Yi Jiao, Li-Fang Lu, Man Xiao, Han Wang

{"title":"Thrombopoietin ameliorates doxorubicin-induced toxicities in H9c2 myocardiocytes by inhibiting oxidative stress through the SIRT1/p38 MAPK signaling pathway","authors":"Xu-Han Zuo, Yu Huang, Bo-Cen Chen, Mingyue Zhu, Caicai Zhang, Han-Yi Jiao, Li-Fang Lu, Man Xiao, Han Wang","doi":"10.4103/apjtb.apjtb_346_24","DOIUrl":"https://doi.org/10.4103/apjtb.apjtb_346_24","url":null,"abstract":"Objective: To explore whether thrombopoietin can exert a protective effect against doxorubicin-induced cardiotoxicity by modulating the sirtuin 1 (SIRT1) signaling pathway. Methods: H9c2 cell viability was determined by CCK-8 and cardiomyocyte apoptosis was detected by TUNEL assay. The protein expressions of SIRT1 and p38 MAPK were measured by Western blot. RT-qPCR was also used to determine S1RT1 mRNA expression. In addition, intracellular reactive oxygen species levels and antioxidant enzyme activities were evaluated. Results: Thrombopoietin treatment reversed doxorubicin-induced decline in H9c2 cell viability. It also increased SIRT1 and decreased p-p38 MAPK protein expressions. In addition, thrombopoietin significantly attenuated doxorubicin-induced apoptosis and oxidative stress, and enhanced antioxidant enzyme activities. However, silencing S1RT1 abrogated the protective effects of thrombopoietin, as evidenced by reduced cell viability and increased oxidative stress and reactive oxygen species levels. Conclusions: Thrombopoietin alleviates doxorubicin-induced cardiomyocyte injury by reducing oxidative stress and apoptosis via the SIRT1/p38 MAPK pathway. However, its protective effects need to be further verified in animal tests.","PeriodicalId":8560,"journal":{"name":"Asian Pacific journal of tropical biomedicine","volume":"14 9","pages":"410-416"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Recent advances on pathogenesis, diagnosis, prevention, immunological aspects, and vectors of dengue: A review 登革热的发病机制、诊断、预防、免疫学和媒介研究进展综述

IF 1.7 4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2023-08-01 DOI: 10.4103/2221-1691.383687

MaheshChandra Sahu, RakeshKumar Samantaray, Aditi Pal, Sanghamitra Pati

引用次数: 0

Hydroxysafflor yellow A protects against thioacetamide-induced liver fibrosis in rats via suppressing proinflammatory/fibrogenic mediators and promoting hepatic stellate cell senescence and apoptosis 羟基红花黄色素A通过抑制促炎/纤维化介质和促进肝星状细胞衰老和凋亡来对抗硫代乙酰胺诱导的大鼠肝纤维化

IF 1.7 4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2023-08-01 DOI: 10.4103/2221-1691.383689

SayedH. Seif el-Din, OlfatA. Hammam, ShahiraM. Ezzat, Samira Saleh, MarwaM. Safar, WalaaH. El-Maadawy, NaglaaM. El-Lakkany

引用次数: 0

Phellinus igniarius ameliorates renal aging in a rat model of focal and segmental glomerulosclerosis 在局灶性和节段性肾小球硬化大鼠模型中，白藜芦醇可改善肾脏老化

IF 1.7 4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2023-08-01 DOI: 10.4103/2221-1691.383690

Ru-Chun Yang, Zhou-Ting Wang, Yue-Wen Tang, Feng Wan, Yan Guo, Jie Zheng, You-Gui Li, Wei-Ming He

引用次数: 0

Aqueous extract of Protaetia brevitarsis larvae increases mTOR-mediated growth rate in zebrafish larvae 短尾原毛滴虫幼虫的水提取物提高斑马鱼幼虫mTOR介导的生长速率

IF 1.7 4区医学

Asian Pacific journal of tropical biomedicine Pub Date : 2023-08-01 DOI: 10.4103/2221-1691.383688

Gi-Young Kim, J. Jayasingha, KyoungTae Lee, YungHyun Choi

引用次数: 0