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Asian Journal of Biochemistry最新文献

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Effect of Melamine Adulteration on Body Weight, Liver and Kidney Histology and Liver Function Indices of Albino Rats 三聚氰胺掺入对白化大鼠体重、肝肾组织学及肝功能指标的影响
Asian Journal of Biochemistry Pub Date : 2015-03-01 DOI: 10.3923/AJB.2015.125.131
S. Shehu, R. Wasagu, M. Lawal, A. K. Albert
{"title":"Effect of Melamine Adulteration on Body Weight, Liver and Kidney Histology and Liver Function Indices of Albino Rats","authors":"S. Shehu, R. Wasagu, M. Lawal, A. K. Albert","doi":"10.3923/AJB.2015.125.131","DOIUrl":"https://doi.org/10.3923/AJB.2015.125.131","url":null,"abstract":"","PeriodicalId":8510,"journal":{"name":"Asian Journal of Biochemistry","volume":"40 1","pages":"125-131"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73250739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Antioxidant Activity, DNA and Cellular Protective Effect of Honey from Srilanka 斯里兰卡蜂蜜的抗氧化活性、DNA和细胞保护作用
Asian Journal of Biochemistry Pub Date : 2015-03-01 DOI: 10.3923/AJB.2015.106.116
M. Sherin, B. Benny, S. Ashadevi
{"title":"Antioxidant Activity, DNA and Cellular Protective Effect of Honey from Srilanka","authors":"M. Sherin, B. Benny, S. Ashadevi","doi":"10.3923/AJB.2015.106.116","DOIUrl":"https://doi.org/10.3923/AJB.2015.106.116","url":null,"abstract":"Honey is a natural product produced by honey bees. Its value is increasing in food industry because of its nutritional and medicinal properties. Besides this, it also contains certain volatile chemicals, phenolic acids, flavonoids and carotenoid like substances which play a major role in antioxidant properties. The composition of honey depends on the plant species visited by the honeybees. The present study was carried out to evaluate the antioxidant activity of Srilankan honey and its role in protecting DNA and erythrocytes from free radical induced oxidative damage. Total phenolic, flavonoid and proanthocyanidin content present in honey was measured, in addition antioxidant assays like 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, reducing power, β-carotene linoleic acid assay, α-glucosidase enzyme inhibition assay, superoxide anion radical scavenging activity and ability of chelating ferrous ions were also studied.","PeriodicalId":8510,"journal":{"name":"Asian Journal of Biochemistry","volume":"8 1","pages":"106-116"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88382651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Antimicrobial Assay for the Volatile Oils of Chromolaena odorata and its Inhibition against the Partially Purified and Characterized Extracellular Protease of Pseudomonas aeruginosa 臭臭草挥发油的抑菌作用及其对铜绿假单胞菌胞外蛋白酶的抑制作用
Asian Journal of Biochemistry Pub Date : 2015-03-01 DOI: 10.3923/AJB.2015.93.105
S. Adeola, O. Folorunso, G. O. Raimi, A. Akinsiku
{"title":"Antimicrobial Assay for the Volatile Oils of Chromolaena odorata and its Inhibition against the Partially Purified and Characterized Extracellular Protease of Pseudomonas aeruginosa","authors":"S. Adeola, O. Folorunso, G. O. Raimi, A. Akinsiku","doi":"10.3923/AJB.2015.93.105","DOIUrl":"https://doi.org/10.3923/AJB.2015.93.105","url":null,"abstract":"","PeriodicalId":8510,"journal":{"name":"Asian Journal of Biochemistry","volume":"33 1","pages":"93-105"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75103967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Effect of Mn2+ on Glutathione-S-Transferase Activity of Human Ejaculated Spermatozoa Mn2+对人射精精子谷胱甘肽- s转移酶活性的影响
Asian Journal of Biochemistry Pub Date : 2015-03-01 DOI: 10.3923/AJB.2015.117.124
Kuldeep Kaushik, P. K. Mittal, N. Kalla
{"title":"Effect of Mn2+ on Glutathione-S-Transferase Activity of Human Ejaculated Spermatozoa","authors":"Kuldeep Kaushik, P. K. Mittal, N. Kalla","doi":"10.3923/AJB.2015.117.124","DOIUrl":"https://doi.org/10.3923/AJB.2015.117.124","url":null,"abstract":"This study was undertaken to monitor the effect of 0.1 mM Mn2+ on the glutathione s-transferase activity (GST) in nicotine treated and untreated spermatozoa samples and to calculate the Michalis-Menten kinetic parameter of the reaction. Semen samples were collected from healthy volunteer donors after semen analysis, sample was subjected for preparation of 10,000 g supernatant. The enzymatic activity has been performed using 1-chloro, 2-4 dinitrobenzene (CDNB) as a substrate. The 0.1 mM Mn2+ supplementation was found to decrease the GST activities significantly (p<0.001). CDNB-GSH conjugate formation were almost significantly (p<0.05) lowered down with the addition of 0.1 mM Mn2+ to nicotine treated samples. The substrate affinity constant (km) of the reaction was found to be 44.44 μM. It remained unchanged throughout the Vmax which was considerably lowered upon Mn2+ addition to the spermatozoal samples. The data was transformed to Lineweaver-Burk 1/v vs 1/[S] plot which indicate the behavior of this enzyme was non-competitive in nature.","PeriodicalId":8510,"journal":{"name":"Asian Journal of Biochemistry","volume":"114 1","pages":"117-124"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79338546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Production and characterization of thermotolerant-organic solvent resistant acidic protease by Pseudomonas aeruginosa RGSS-09 isolated from dairy sludge. 奶牛场污泥中铜绿假单胞菌RGSS-09耐热有机耐溶剂酸性蛋白酶的制备及特性研究。
Asian Journal of Biochemistry Pub Date : 2015-02-01 DOI: 10.3923/AJB.2015.52.66
R. Gaur, S. Tiwari, Sheffalika Singh
{"title":"Production and characterization of thermotolerant-organic solvent resistant acidic protease by Pseudomonas aeruginosa RGSS-09 isolated from dairy sludge.","authors":"R. Gaur, S. Tiwari, Sheffalika Singh","doi":"10.3923/AJB.2015.52.66","DOIUrl":"https://doi.org/10.3923/AJB.2015.52.66","url":null,"abstract":"Fifteen bacterial strains isolated from the dairy soil samples for acidic protease production. Among them, culture RGSS-09 was adjudged as the best protease producer and was identified as Pseudomonas aeruginosa RGSS-09 from MTCC Chandigarh. The isolate showed maximum protease production (1256 U mLG) with 2.5 g LG biomass production in the presence of wheat bran and yeast extract at 37°C pH 6.5 within 48 h of incubation. Pseudomonas aeruginosa RGSS-09 also showed enhanced activity and stability in presence of Ca, Mg, Tween-20, Tween-40, triton-X-100 and SDS at 5 and 10 mM concentration. The enzyme was also stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-haxane, n-butanol and cyclohexane, respectively. The presence of benzene, methanol and ethanol marginally reduced the protease stability, respectively. The enzyme was showed it 100% activity at 75°C and pH 7.5 with 110 and 100% stability at 70°C and pH 7.0, respectively. The purity of the enzyme was confirmed by the presence of a single band on SDS-PAGE and its molecular weight was approximately 42 kDa. Pseudomonas aeruginosa can be used profitably for the large scale production of protease to meet the present day demand of the industrial sector.","PeriodicalId":8510,"journal":{"name":"Asian Journal of Biochemistry","volume":"13 1","pages":"52-66"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89365775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Effect of Bioconjugation of Oligodeoxynucleotides with Lutein on N-nitrosodimethylamine Induced Fibrosis in Cultured Human Retinal Pigment Epithelial Cells 寡脱氧核苷酸与叶黄素生物偶联对n -亚硝基二甲胺诱导的人视网膜色素上皮细胞纤维化的影响
Asian Journal of Biochemistry Pub Date : 2015-02-01 DOI: 10.3923/AJB.2015.78.85
M. Kumar, K. Swetha
{"title":"Effect of Bioconjugation of Oligodeoxynucleotides with Lutein on N-nitrosodimethylamine Induced Fibrosis in Cultured Human Retinal Pigment Epithelial Cells","authors":"M. Kumar, K. Swetha","doi":"10.3923/AJB.2015.78.85","DOIUrl":"https://doi.org/10.3923/AJB.2015.78.85","url":null,"abstract":"","PeriodicalId":8510,"journal":{"name":"Asian Journal of Biochemistry","volume":"26 1","pages":"78-85"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85058629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Protective effects of camellia sinensis leaf extract against carbon tetrachloride-induced liver injury in rats 茶树叶提取物对四氯化碳致大鼠肝损伤的保护作用
Asian Journal of Biochemistry Pub Date : 2015-02-01 DOI: 10.3923/AJB.2015.86.92
H. Wakawa, Ira
{"title":"Protective effects of camellia sinensis leaf extract against carbon tetrachloride-induced liver injury in rats","authors":"H. Wakawa, Ira","doi":"10.3923/AJB.2015.86.92","DOIUrl":"https://doi.org/10.3923/AJB.2015.86.92","url":null,"abstract":"The present study appraised the hepatoprotective effects of aqueous leaf extract of Camellia sinensis against carbon tetrachloride (CCl4)-induced liver injury in rats. The 100 and 200 mg kg-1 b.wt. of Camellia sinensis and 100 mg kg-1 b.wt. Silymarin was administered orally to separate groups of rats for 7 days prior to CCl4 administration. A significant decrease (p<0.05) was observed in both the groups treated with 100 and 200 mg kg-1 b.wt. of the leaf extract and Silymarin on the levels of the enzymes and non-enzyme markers of tissue damage and lipid peroxidation, with no significant changes in the relative organ weight of the treated groups. This result shows that the leaf extract of Camellia sinensis contains some phytochemical(s) that can protect the liver against CCl4-induced injury in rats.","PeriodicalId":8510,"journal":{"name":"Asian Journal of Biochemistry","volume":"106 1","pages":"86-92"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75668381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Sub-Chronic Oral Esculetin (6, 7-Dihydroxy-Coumarin) Exposure in Male Wistar Rats: Effect on Some Serum Functions and Organ Histology 雄性Wistar大鼠亚慢性口服Esculetin(6,7 -二羟基香豆素)暴露:对某些血清功能和器官组织学的影响
Asian Journal of Biochemistry Pub Date : 2015-02-01 DOI: 10.3923/AJB.2015.67.77
Anthony Cemaluk C. Egbuonu, Ambrose E. Ogbu, L. Ezeanyika
{"title":"Sub-Chronic Oral Esculetin (6, 7-Dihydroxy-Coumarin) Exposure in Male Wistar Rats: Effect on Some Serum Functions and Organ Histology","authors":"Anthony Cemaluk C. Egbuonu, Ambrose E. Ogbu, L. Ezeanyika","doi":"10.3923/AJB.2015.67.77","DOIUrl":"https://doi.org/10.3923/AJB.2015.67.77","url":null,"abstract":"","PeriodicalId":8510,"journal":{"name":"Asian Journal of Biochemistry","volume":"31 1","pages":"67-77"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74075294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Identifying the Putative Interacting Partners of the Heat Shock Protein, HtpG through Phage Display Library Screening 通过噬菌体展示库筛选确定热休克蛋白HtpG可能的相互作用伙伴
Asian Journal of Biochemistry Pub Date : 2015-01-01 DOI: 10.3923/AJB.2015.1.16
M. Youssef, M. Al-Omair
{"title":"Identifying the Putative Interacting Partners of the Heat Shock Protein, HtpG through Phage Display Library Screening","authors":"M. Youssef, M. Al-Omair","doi":"10.3923/AJB.2015.1.16","DOIUrl":"https://doi.org/10.3923/AJB.2015.1.16","url":null,"abstract":"","PeriodicalId":8510,"journal":{"name":"Asian Journal of Biochemistry","volume":"21 1","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73874329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Effect of Celosia argentea F. Cristata (L.) Schinz. on Prostate Specific Antigen, Antioxidant Status and Hematological Parameters in Rats Induced with Benign Prostate Hyperplasia 鸡冠花(L. argenttea F. Cristata)的药效Schinz。良性前列腺增生大鼠前列腺特异性抗原、抗氧化状态及血液学指标的影响
Asian Journal of Biochemistry Pub Date : 2015-01-01 DOI: 10.3923/AJB.2015.42.51
E. Iweala, J. Ogidigo
{"title":"Effect of Celosia argentea F. Cristata (L.) Schinz. on Prostate Specific Antigen, Antioxidant Status and Hematological Parameters in Rats Induced with Benign Prostate Hyperplasia","authors":"E. Iweala, J. Ogidigo","doi":"10.3923/AJB.2015.42.51","DOIUrl":"https://doi.org/10.3923/AJB.2015.42.51","url":null,"abstract":"","PeriodicalId":8510,"journal":{"name":"Asian Journal of Biochemistry","volume":"6 1","pages":"42-51"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87799806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
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