Asian Journal of Biochemistry, Genetics and Molecular Biology最新文献

Effects of Eleophorbia drupifera Leaves Extract on Some Biochemical and Haematological Parameters of Albino Rats 荸荠叶提取物对白化大鼠某些生化指标和血液指标的影响

Asian Journal of Biochemistry, Genetics and Molecular Biology Pub Date : 2024-08-09 DOI: 10.9734/ajbgmb/2024/v16i8400

Nyah, Nkereuwem U., Obosi, Ekemini J., E. J., Ukpong, Etiowo G, Chukwuemeka, Iheanyichukwu E

{"title":"Effects of Eleophorbia drupifera Leaves Extract on Some Biochemical and Haematological Parameters of Albino Rats","authors":"Nyah, Nkereuwem U., Obosi, Ekemini J., E. J., Ukpong, Etiowo G, Chukwuemeka, Iheanyichukwu E","doi":"10.9734/ajbgmb/2024/v16i8400","DOIUrl":"https://doi.org/10.9734/ajbgmb/2024/v16i8400","url":null,"abstract":"Water and ethanol-water extracts of Eleophorbia drupifera leaves were administered orally in graded doses of 0.5ml (147.06mg/kg body weight), 1.0ml (303.3 mg/kg body weight) water extract and 0.5ml (157.66 mg/kg body weight), 1.0ml (301.72 mg/kg body weight) ethanol-water extract on experimental animals for 3 weeks. The effect of the extracts on some biochemical and haematological parameters were evaluated in albino rats. At the end of 21 days, the growth response was higher in the test groups than in the control (p <0.05). The Aspartate transaminase (AST) and Alanine transaminase (ALT) levels of the test groups were significantly lower than the control (p <0.05) and total bilirubin showed significantly high values in test groups when compared with the control (p<0.05). The White blood cell (WBC) was significantly increased while the Red blood cell (RBC) was decreased when compared with the control (p<0.05). There were no significant changes in Packed cell volume (PCV) and haemoglobin (Hb) of the experimental animals (p<0.05). There was no significant difference between the effects of the water extract and that of the ethanol-water extract on the albino rats (p<0.05). The results suggest that the leaf extract of Eleophorbia drupifera may possess the capacity to stimulate appetite and there may be no adverse effect associated with the use of this extract in phytotherapy.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"45 33","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141924100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Differentiation of Indian Zebu Cattle (Bos tauraus) Breeds Using Random Oligonucleotide Primers (RAPD-PCR) in Amravati Region, Maharashtra, India 使用随机寡核苷酸引物（RAPD-PCR）对印度马哈拉施特拉邦阿姆拉瓦蒂地区的印度斑牛（Bos tauraus）品种进行遗传分化

Asian Journal of Biochemistry, Genetics and Molecular Biology Pub Date : 2024-07-26 DOI: 10.9734/ajbgmb/2024/v16i8399

Nilima J. Tayde, H. P. Nandurkar, Nandakishor E. Warghat

{"title":"Genetic Differentiation of Indian Zebu Cattle (Bos tauraus) Breeds Using Random Oligonucleotide Primers (RAPD-PCR) in Amravati Region, Maharashtra, India","authors":"Nilima J. Tayde, H. P. Nandurkar, Nandakishor E. Warghat","doi":"10.9734/ajbgmb/2024/v16i8399","DOIUrl":"https://doi.org/10.9734/ajbgmb/2024/v16i8399","url":null,"abstract":"In India, insufficient characterization skills and extensive crossbreeding of cattle have led to the loss of several breeds. The purpose of this work is to find polymorphic primers for identifying Zebu cattle breeds. Random crossbreeding has resulted in genetic losses among the Gaolao, Krishna Valley, and Hallikar breeds. Genetic characterization and cryopreserving semen are critical components of conservation and breeding initiatives. The study highlights the urgent need for effective genetic characterization of Zebu cattle breeds in India, identifying 8 polymorphic and 4 monomorphic primers through RAPD-PCR. These findings are crucial for accurate breed identification, which is essential for conservation efforts and informed breeding strategies to preserve genetic diversity and prevent the decline of indigenous breeds like Gaolao, Krishna Valley, and Hallikar. Genomic DNA was isolated from whole blood using a DNA extraction kit and amplified with RAPD-PCR. A total of 12 primers were evaluated, and agarose gel electrophoresis revealed that eight (OPA-09, OPA-15, OPB-13, OPB-07, OPB- 05, OPA-13, OPA-18, and OPA-01) were polymorphic and four (OPB-06, OPA-04, OPB-03, and OPA-02) were monomorphic. Polymorphic primers demonstrated relatedness between some of the available breeds. Primers OPA-09 and OPA-15 showed a higher degree of polymorphism than primers OPB-13, OPB-07, OPB-05, OPA-13, OPA-18, and OPA-01, indicating that primers OPA-09 and OPA-15 may successfully identify various cattle breeds.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141801476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial Resistance and Phenotypic Detection of Extended Spectrum Beta-Lactamase in Escherichia coli from Children with Cases of Diarrhea in Nasarawa-South, Nasarawa State, Nigeria 尼日利亚纳萨拉瓦州纳萨拉瓦-南区腹泻病例儿童中大肠埃希菌的抗菌药耐药性和扩展谱β-内酰胺酶表型检测

Asian Journal of Biochemistry, Genetics and Molecular Biology Pub Date : 2024-07-25 DOI: 10.9734/ajbgmb/2024/v16i8398

Zakou At, Ngwai Yb, Nkene Ih, I. D, Abimiku Rh, Ekeleme Ik

{"title":"Antimicrobial Resistance and Phenotypic Detection of Extended Spectrum Beta-Lactamase in Escherichia coli from Children with Cases of Diarrhea in Nasarawa-South, Nasarawa State, Nigeria","authors":"Zakou At, Ngwai Yb, Nkene Ih, I. D, Abimiku Rh, Ekeleme Ik","doi":"10.9734/ajbgmb/2024/v16i8398","DOIUrl":"https://doi.org/10.9734/ajbgmb/2024/v16i8398","url":null,"abstract":"Aims: This study investigates the antimicrobial resistance and phenotypic detection of extended-spectrum beta-lactamase in Escherichia coli from children with cases of diarrhea in Nasarawa-south, Nasarawa State, Nigeria. \u0000Place and Duration of Study: Nasarawa South, Nigeria, in 2023. \u0000Methodology: A total of 251 non-duplicate E. coli isolates were collected from Dalhatu Araf Specialist Hospital Lafia (DASHL), General Hospitals Awe (GHA), General Hospital Doma (GHD), General Hospital Obi (GHO) and General Hospital Keana (GHK); and confirmed using standard microbiological methods. Antimicrobial susceptibility testing and phenotypic detection of ESBL production in the isolates were carried out using disc diffusion methods. \u0000Results: The isolates were highly resistant (74.0-100.0%) to ampicillin, amoxicillin/clavulanic acid and sulfamethoxazole/trimethoprim, ceftriaxone and streptomycin in all the selected hospitals, but less resistance (0-7.8%) to Cefotaxime and Ceftazidime. All the antibiotic resistance isolates were multiple antibiotic resistance (MAR) with MAR indices above 0.2; and more than 90.0% in all hospitals were multidrug resistance (MDR) isolates. The occurrence of ESBL producing isolates was highest in DASHL (7.8%), GHK and GHD (2.0%) but none of the isolates from GHA were ESBL producers. \u0000Conclusion: Extended-spectrum third generation cephalosporins, cefotaxime and ceftazidime were very effective against the isolates, even though most of the isolates were multidrug resistant. The molecular diversity of the ESBL-producing isolates is being investigated.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141802891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Role of Probiotics in Advancement of Combating Physical Abnormalities and Diseases: A Systematic Perspective Analysis 益生菌在防治身体异常和疾病方面的新作用：系统性透视分析

Asian Journal of Biochemistry, Genetics and Molecular Biology Pub Date : 2024-07-16 DOI: 10.9734/ajbgmb/2024/v16i8397

Mst. Tania Khatun, Forkanul Hoque, Naeema Salatia Hoque, Md. Sakhawat Hossain, Md. Ashraful Alam, Sabrina Afrin, Tanjina Nasrin Eva, Rahima Tanbin Tama

{"title":"Emerging Role of Probiotics in Advancement of Combating Physical Abnormalities and Diseases: A Systematic Perspective Analysis","authors":"Mst. Tania Khatun, Forkanul Hoque, Naeema Salatia Hoque, Md. Sakhawat Hossain, Md. Ashraful Alam, Sabrina Afrin, Tanjina Nasrin Eva, Rahima Tanbin Tama","doi":"10.9734/ajbgmb/2024/v16i8397","DOIUrl":"https://doi.org/10.9734/ajbgmb/2024/v16i8397","url":null,"abstract":"Probiotics are live microorganisms, primarily bacteria that give the host health benefits when given in sufficient amounts. It works in various defensive mechanisms, including releasing antimicrobial compounds, competitive adhesion to the mucosa and epithelium, fortifying the gut epithelial barrier and regulating the immune system. Probiotics have a considerable effect in reducing allergies and gastrointestinal problems like diarrhoea and ulcers. Probiotics are quite beneficial for managing and preventing complex diseases including diabetes, cardiovascular diseases, anaemia, urogenital diseases, dental caries, URTI, cancers and various abnormalities like obesity, depression, etc. Nowadays, Antibiotic resistance is a global challenge associated with high morbidity and mortality. The current lack of efficacious treatments necessitates the creation of innovative therapeutic approaches and substitute antimicrobial therapies. So, natural products or probiotics would be a better option for treating human health. Therefore, we systematically reviewed the sources, including Google Scholar, PubMed, Science Direct, Jstor and Base; studies only about human health were included here. All types of animal diseases and articles before 1990 are excluded. A total of 6,410 articles were identified and 300 articles were taken for further evaluation based on context. However, Probiotics have already become a successful therapeutic intervention strategy among physicians. This investigation covers a comprehensive overview of the current state of probiotic research which comprises a wide range of topics including sourcing, mode of action, probiotic strains, isolating procedures and the role of probiotics. \u0000Graphical abstract \u0000","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"86 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141643035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectral Characterization of Canarium ovatum Engl. (Pili) Pulp Extract from Allen, Northern Samar, Philippines 菲律宾北萨马省艾伦的 Canarium ovatum Engl.

Asian Journal of Biochemistry, Genetics and Molecular Biology Pub Date : 2024-07-13 DOI: 10.9734/ajbgmb/2024/v16i7396

Harley Nico L. Jazmin, Flyndon Mark S. Dagalea, K. M. Cui-Lim

{"title":"Spectral Characterization of Canarium ovatum Engl. (Pili) Pulp Extract from Allen, Northern Samar, Philippines","authors":"Harley Nico L. Jazmin, Flyndon Mark S. Dagalea, K. M. Cui-Lim","doi":"10.9734/ajbgmb/2024/v16i7396","DOIUrl":"https://doi.org/10.9734/ajbgmb/2024/v16i7396","url":null,"abstract":"Canarium ovatum (Pili) Pulp was widely cultivated in the town of Allen, Northern Samar, Philippines. In Allen, Northern Samar produces 60 to 100 kilograms per year every tree. The pili can used in various aspects like oil and soap production, fuel, sweet candies and bread stuffing, and used in traditional medicines for its nutritional benefits. Thus, this study focuses on characterizing the Pili extract using Ultraviolet visible (UV-vis) spectrophotometer. Physical properties were determined in terms of boiling point, color, density, odor, pH, and solubility. Likewise, the nutritional content was determined in terms of ash content, carbohydrates, crude fats, crude protein, and moisture content. Lastly, to characterize the Pili pulp, a UV-vis spectrophotometer was used to determine and quantify the nutraceutical content of the plant sample. Results showed that, Pili pulp has a lower boiling point than water, an orange color, with unpleasant odor, density less than water, slightly acidic, and exhibited a polar nature. Subsequently, proximate analysis revealed that ash content has a high mineral concentration and, also, carbohydrates can contribute for energy source. The presence of crude fats indicates essential fatty acids necessary for maintaining cellular structure. On the other hand, crude protein offers health diet as well as low moisture content of plant sample implies a longer shelf-life and reduced microbial growth. Correspondingly, spectra analysis of UV-vis indicates among phytochemical screening tested for positive results and promoting possible applications in developing natural remedies. Therefore, the findings provide evidence as therapeutic advantages for characterization and evaluation which produce health and economic benefits for individuals, businesses, farmers and government agencies.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"63 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141651781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Garcinia Kola in Ivermectin-Induced Toxicity in the Hematology of Treated Rats (Rattus Novergicus) 加西可拉对伊维菌素诱导的大鼠血液学毒性的影响

Asian Journal of Biochemistry, Genetics and Molecular Biology Pub Date : 2024-07-12 DOI: 10.9734/ajbgmb/2024/v16i7395

B. Okolonkwo, Godspower Ikechi Achi, Erasmus Martha A, Suama Promise, Patrick Williams, Osuosa Atoukaritou, Esther Okagbe Ogbugo

{"title":"Effect of Garcinia Kola in Ivermectin-Induced Toxicity in the Hematology of Treated Rats (Rattus Novergicus)","authors":"B. Okolonkwo, Godspower Ikechi Achi, Erasmus Martha A, Suama Promise, Patrick Williams, Osuosa Atoukaritou, Esther Okagbe Ogbugo","doi":"10.9734/ajbgmb/2024/v16i7395","DOIUrl":"https://doi.org/10.9734/ajbgmb/2024/v16i7395","url":null,"abstract":"Garcinia kola (GK) is prized in African herbal medicine for its diverse uses, including social, economic, and medicinal purposes. This study examined the dose-dependent toxicity of ivermectin and the protective effects of Garcinia kola extract on the hematological parameters of Wistar rats. A total of 32 Wistar Rats (120 – 150g) were randomly chosen from PAMO University of Medical Sciences' Animal House. They were acclimatized for two weeks. Divided into 4 main groups (A, B, C, D), which were further subdivided into 2 subgroups each (A1, A2, B1, B2, C1, C2, D1 and D2) with each subgroup containing 4 rats. Subgroup A: negative control (0 mg/kg BW of ivermectin, no Garcinia kola). Subgroup A2: positive control (0 mg/kg BW of ivermectin and 20 mg/kg/ml Garcinia kola). Test Subgroups 1 (B1, C1 and D1) received respectively doses of 10 mg/kg/ml, 20 mg/kg/ml, and 40 mg/kg/ml of Ivermectin intraperitoneally, Subgroups 2 (B2, C2 and D2) received respectively the same doses followed by oral administration of Garcinia kola at 20 mg/kg BW of rat per ml daily for 7 days, starting 24 hours after toxicity induction. On the eighth day, each rat was anesthetized with chloroform. 5mls of blood was collected via cardiac puncture into EDTA bottle for the analysis of the CBC parameters. ANOVA was used to test the hypotheses at alpha = 0.05. The result showed a significant dose-dependent difference in anaemia indicators in ivermectin induction but there was no significant change in after bitter kola treatment. The study has shown that ivermectin inflicted haematological toxicity particicular in particular in anaemic indicators but bitter kola couldn’t ameliorate the effect of the toxicity.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"3 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141654497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Genotoxicity by Comet Assay in Tissues of Clarias gariepinus Exposed to Cassava Effluent 用彗星试验评估暴露于木薯废水中的石斑鱼组织的遗传毒性

Asian Journal of Biochemistry, Genetics and Molecular Biology Pub Date : 2024-07-09 DOI: 10.9734/ajbgmb/2024/v16i7394

D. Olorunfemi, O. Orororo, N. E. Iloduba, E. Osioma, E. D. Kpomah, Ogheneovo L. Osio

{"title":"Evaluation of Genotoxicity by Comet Assay in Tissues of Clarias gariepinus Exposed to Cassava Effluent","authors":"D. Olorunfemi, O. Orororo, N. E. Iloduba, E. Osioma, E. D. Kpomah, Ogheneovo L. Osio","doi":"10.9734/ajbgmb/2024/v16i7394","DOIUrl":"https://doi.org/10.9734/ajbgmb/2024/v16i7394","url":null,"abstract":"Untreated wastewater discharge into waterbodies poses a number of risks, including the potential for aquatic organisms' DNA to be damaged. Using the comet assay, this study assessed the genotoxic impact of cassava wastewater on the gonads, liver, and gills of post-juvenile Clarias gariepinus. The post-juvenile C. gariepinus fish were acquired from a fish farm in Edo State, Nigeria, and were subjected to different concentrations of cassava wastewater (0.2%, 0.3%, 0.5%, and 0.7%) for a duration of 96 hours. In contrast to the typical actions seen in the control groups, the fish exposed to the effluent exhibited restless, erratic movements, and gasping for air. The first set of repetitions showed that catfish mortality increased with concentration; after 96 hours, exposure to the lowest concentration (0.2%) and the highest concentration (0.7%) of cassava wastewater caused 10% and 40% of the fish to die, respectively. The second set of repetitions showed that catfish mortality increased with increasing concentration; after 96 hours, exposure to the lowest concentration (0.2%) and the highest concentration (0.7%) of cassava wastewater resulted in 20% and 50% catfish mortality, respectively. Following the exposure time, the fish were brought to the lab where their gonads, liver, and gills were removed in order to use the comet assay for genotoxic assessment. The genotoxins present in the wastewater can be the reason for the DNA impairment found in the genotoxic assessment result at different effluent concentrations. The investigation also showed that DNA damage increased with increasing concentration, indicating a dose-dependent genotoxic effect of cassava wastewater on post-juveniles of C. gariepinus. This research demonstrates the critical importance of treating wastewater before to discharge.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141664859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GC-MS Charaterization of Phytochemicals and Anti Microbial Properties of Chromolaena odorata Leaf Harvested from South Eastern Nigeria 从尼日利亚东南部采摘的 Chromolaena odorata 叶片的植物化学物质和抗微生物特性的气相色谱-质谱分析（GC-MS Charateraterization of Phytochemicals and Anti Microbial Properties of Chromolaena odorata Leaf Harvested from South Eastern Nigeria

Asian Journal of Biochemistry, Genetics and Molecular Biology Pub Date : 2024-07-06 DOI: 10.9734/ajbgmb/2024/v16i7393

Emeziem D.M, Iwu I C

引用次数: 0

A Computational Modeling Study on the Biomolecular Interactions of the Phytoconstituents of Nigella sativa with Anti-Apoptotic Proteins Mcl-1 and Bcl-x1 黑麦草植物成分与抗凋亡蛋白 Mcl-1 和 Bcl-x1 的生物分子相互作用的计算模型研究

Asian Journal of Biochemistry, Genetics and Molecular Biology Pub Date : 2024-06-04 DOI: 10.9734/ajbgmb/2024/v16i7389

O. B. Oladapo, Toheeb A. Jumah, Justine U. Egbe, Jude O. Uzoechina, A. E. Fakayode, Muhammad I. Adeyemi, O. Olaniyan, Chimunda A. Solomon, Zion O. Oluwasegun, Damilola Olatunde, U. Arunsi, Samuel O Olubode, P. A. Akinnusi, T. Balogun, Sunday B. Akinde

{"title":"A Computational Modeling Study on the Biomolecular Interactions of the Phytoconstituents of Nigella sativa with Anti-Apoptotic Proteins Mcl-1 and Bcl-x1","authors":"O. B. Oladapo, Toheeb A. Jumah, Justine U. Egbe, Jude O. Uzoechina, A. E. Fakayode, Muhammad I. Adeyemi, O. Olaniyan, Chimunda A. Solomon, Zion O. Oluwasegun, Damilola Olatunde, U. Arunsi, Samuel O Olubode, P. A. Akinnusi, T. Balogun, Sunday B. Akinde","doi":"10.9734/ajbgmb/2024/v16i7389","DOIUrl":"https://doi.org/10.9734/ajbgmb/2024/v16i7389","url":null,"abstract":"Cancer is the second leading cause of mortality worldwide. The strictly controlled physiological process of apoptosis is required for immune system function, maintenance of tissue homeostasis, and appropriate embryonic development. Anti-apoptotic proteins such as Bcl-xL and Mcl-1 are potent new anticancer targets. The inhibitory effect of the apparently therapeutic plant Nigella sativa on these targets is investigated using a molecular modeling approach in this work. From the molecular docking, we predict that seven compounds; apigenin, chlorogenic acid, hesperidin, quercetin, quercitrin, kaempferol, and rutin may have greater inhibitory potential against the target protein. These compounds have higher docking scores thus indicating higher binding affinities when compared to co-crystallized compounds. The co-compounds were crystallized with the standards, which served as the baselines for comparison studies. This result shows that that Nigella sativa compounds may be a potential anticancer drug that targets the anti-apoptotic protein. Targeting anti-apoptotic proteins provides clinical studies with the opportunity to evaluate for possible anti-cancer potential in the plant via other experimental models like rats and cancer cell lines. Using phytomedicines can equally augment existing therapy to provide synergistic anti-cancer effect when combined with existing drugs thereby enhancing therapy efficacy and because medicinal plants has lots of phytoconstituents, its use in this research can provide benefits of targeting multiple anti-apoptotic proteins thereby enhancing therapeutic effect unlike some conventional drugs that are mostly single targeting.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"18 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141266842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pattern and Dynamics of Genetic Stock of Freshwater Fishes Using Various Molecular Markers for Their Conservation Management Concerns in the Indian Riverine System 利用各种分子标记研究印度河水系淡水鱼类遗传种群的模式和动态，关注其保护管理问题

Asian Journal of Biochemistry, Genetics and Molecular Biology Pub Date : 2024-06-03 DOI: 10.9734/ajbgmb/2024/v16i7388

Gayatri Batham, R.K. Garg

{"title":"Pattern and Dynamics of Genetic Stock of Freshwater Fishes Using Various Molecular Markers for Their Conservation Management Concerns in the Indian Riverine System","authors":"Gayatri Batham, R.K. Garg","doi":"10.9734/ajbgmb/2024/v16i7388","DOIUrl":"https://doi.org/10.9734/ajbgmb/2024/v16i7388","url":null,"abstract":"India has a world's richest, most abundant and most promising inland fisheries resources with variety fishes in tributaries, streams, canals, lakes, ponds, and reservoirs. There are several significant river systems in India, including the Cauvery, Tapi, Narmada, Krishna, Indus, Brahmaputra, Ganga, Mahanadi, and Godavari which showing shrinkage of fish fauna because of environmental disturbance, human interference and human threats. In order to conserve the fish diversity, the molecular markers are helpful in determining genetic diversity, gene polymorphism and gene flows from generation to generation. The topic experienced a surge in interest due to the introduction of strong statistical analysis tools and the accessibility of DNA fingerprinting, DNA sequencing techniques and consequently population genetic studies. Some molecular markers, Microsatellite markers, RAPD, Allozymes and mitochondrial (cox1, cytob, ATPase6/8) implications have been discussed in this review which will provide an overview that have been used by scientists to studied population genetic structure and genetic variations at various levels.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"28 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141273042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0