Archivum histologicum Japonicum = Nihon soshikigaku kiroku最新文献_第6页

A karyometrical study of circulating erythroblasts of yolk sac origin in the mouse embryo. 小鼠胚胎卵黄囊来源的循环红细胞的核计量学研究。

Archivum histologicum Japonicum = Nihon soshikigaku kiroku Pub Date : 1986-12-01 DOI: 10.1679/aohc.49.535

K Sasaki, G Matsumura

引用次数: 2

Immunohistochemical studies on the GABAergic system in the rat supraoptic nucleus using the PAP method with an application of electron microscopy. 电镜下应用PAP法对大鼠视上核gaba能系统的免疫组化研究。

Archivum histologicum Japonicum = Nihon soshikigaku kiroku Pub Date : 1986-12-01 DOI: 10.1679/aohc.49.579

K Iijima, K Ohtomo, R Kobayashi, N Kojima

{"title":"Immunohistochemical studies on the GABAergic system in the rat supraoptic nucleus using the PAP method with an application of electron microscopy.","authors":"K Iijima, K Ohtomo, R Kobayashi, N Kojima","doi":"10.1679/aohc.49.579","DOIUrl":"https://doi.org/10.1679/aohc.49.579","url":null,"abstract":"GABAergic neuronal profiles in the supraoptic nucleus of the rat were immunohistochemically identified by using a purified GABA antibody with the peroxidase-antiperoxidase method. The localization of GABA-like immunoreactivity in nerve terminals on the neurosecretory neurons was examined electron microscopically. A few small GABAergic neurons were found inside the supraoptic nucleus while only a very few medium-sized ones were detected in the perinuclear zone. Intrinsic, non-GABAergic small neurons covered by GABAergic neuropil were also detected. The neuropil with GABAergic axo-somatic synapses evenly encompassed unlabeled neurosecretory perikarya throughout the supraoptic nucleus. The GABAergic system seems to inhibit both vasopressin and oxytocin cells. In this area, glia cells showed clear outlines of unlabeled somata around counter-stained nuclei. Blood capillaries in the supraoptic nucleus were only slightly covered with a GABAergic neuropil. Electron microscopic observations demonstrated the presence of GABAergic axo-somatic symmetrical and axo-dendritic asymmetrical synapses on the neurosecretory neurons. GABA-like immunoreactivity was localized on the membranes of microtubules and synaptic vesicles, on the external membranes of the mitochondria, and on the inner leaf of the presynaptic sites. Numerous pairs of non-immunoreactive synapses were arranged along these immunoreactive synapses.","PeriodicalId":8387,"journal":{"name":"Archivum histologicum Japonicum = Nihon soshikigaku kiroku","volume":"49 5","pages":"579-91"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.49.579","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14674410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Three types of photoreceptors in the pineal and frontal organs of frogs: ultrastructure and opsin immunoreactivity. 蛙的松果体和额部器官中三种类型的光感受器:超微结构和视蛋白免疫反应性。

Archivum histologicum Japonicum = Nihon soshikigaku kiroku Pub Date : 1986-12-01 DOI: 10.1679/aohc.49.495

B Vigh, I Vigh-Teichmann

{"title":"Three types of photoreceptors in the pineal and frontal organs of frogs: ultrastructure and opsin immunoreactivity.","authors":"B Vigh, I Vigh-Teichmann","doi":"10.1679/aohc.49.495","DOIUrl":"https://doi.org/10.1679/aohc.49.495","url":null,"abstract":"The pineal complex in frogs (Rana esculenta, R. temporaria, R. tigrina, R. arvalis) was studied by conventional electron microscopy and postembedding rhodopsin immunoelectron microscopy. Three types of photoreceptor cells were found in both the pineal and frontal organs. In the pineal organ, most of the photoreceptors exhibited rhodopsin-immunoreactive outer segments and large inner segments with a large ellipsoid of densely packed mitochondria (\"rod-like\" photoreceptors). A small number of photoreceptors was rhodopsin-immunonegative (\"cone-like\" photoreceptors). In both Rana esculenta and R. temporaria, the latter were either supplied with an oil droplet and an ellipsoid in their inner segment, or they were electron-lucent with a small inner segment without an ellipsoid. In contrast, the frontal organ displayed many immunonegative \"cone-like\" outer segments and few rhodopsin-immunoreactive \"rod-like\" photoreceptors. In both organs, the basal processes of the photoreceptor cells were found to form ribbon-containing axonal pedicles which synapsed with the dendrites of secondary neurons. The latter rarely received any further afferences by conventional synapses. The frog pineal organ is considered a predominantly \"rod-type\" and the frontal organ a \"cone-type\" photosensory organ. The presence of three kinds of pineal/frontal photoreceptors is discussed in connection with the occurrence of different photopigments (rhodopsin/porphyropsin, iodopsin, ultraviolet and/or blue pigments) enabling the animal to discriminate by the pineal complex environmental light in various ranges of the spectrum.","PeriodicalId":8387,"journal":{"name":"Archivum histologicum Japonicum = Nihon soshikigaku kiroku","volume":"49 5","pages":"495-518"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.49.495","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14674408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

Dynamic behavior of ciliated centrioles in rat incisor ameloblasts during cell differentiation. 大鼠门牙成釉细胞分化过程中纤毛中心粒的动态行为。

Archivum histologicum Japonicum = Nihon soshikigaku kiroku Pub Date : 1986-10-01 DOI: 10.1679/aohc.49.437

Y Sasano

引用次数: 9

Surface morphology of the human yolk sac: endoderm and mesothelium. 人卵黄囊的表面形态:内胚层和间皮层。

Archivum histologicum Japonicum = Nihon soshikigaku kiroku Pub Date : 1986-10-01 DOI: 10.1679/aohc.49.483

A Ukeshima, Y Hayashi, T Fujimoto

引用次数: 16

Serial semithin sections in immunohistochemistry: techniques and applications. 免疫组织化学连续半薄切片:技术与应用。

Archivum histologicum Japonicum = Nihon soshikigaku kiroku Pub Date : 1986-10-01 DOI: 10.1679/aohc.49.391

D Grube, Y Kusumoto

{"title":"Serial semithin sections in immunohistochemistry: techniques and applications.","authors":"D Grube, Y Kusumoto","doi":"10.1679/aohc.49.391","DOIUrl":"https://doi.org/10.1679/aohc.49.391","url":null,"abstract":"Immunostaining of semithin sections is a valuable tool in biomedical research; however, this method is rather rarely used by histologists. To overcome the apparent reservations concerning this method, the present report describes a technique which is rather simple, largely standardized, and very useful in investigative endocrinology. The technique includes the following steps: snap-freezing and freeze-drying of tissue specimens, embedding in Araldite, preparation of serial semithin sections, removal of the resin from tissue sections, immunostaining with Sternberger's peroxidase antiperoxidase (PAP) technique, and analyses of the sections by various microscopical techniques which in part optically enhance immunoreactive sites. In addition to a detailed description of the method, examples for its applications are given, including concomitant investigations of the same cells by empirical staining, immunostaining, and fluorescence histochemistry of biogenic monoamines; colocalization of multiple peptides to the same cells and corresponding specificity controls; three-dimensional reconstructions based upon immunostained serial semithin sections; quantitative (computer-assisted) determinations of immunoreactivities. Because of the advantages offered by immunostained serial semithin sections as well as the vast field of applications, the method described is recommended for routine use. Concomitantly this method covers the gap between conventional light microscopy (paraffin sections) and electron microscopy.","PeriodicalId":8387,"journal":{"name":"Archivum histologicum Japonicum = Nihon soshikigaku kiroku","volume":"49 4","pages":"391-410"},"PeriodicalIF":0.0,"publicationDate":"1986-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.49.391","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14667140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 71

The innermost layer of cementum in rat molars: its ultrastructure, development, and calcification. 大鼠臼齿牙骨质最内层:超微结构、发育及钙化。

Archivum histologicum Japonicum = Nihon soshikigaku kiroku Pub Date : 1986-10-01 DOI: 10.1679/aohc.49.459

T Yamamoto

{"title":"The innermost layer of cementum in rat molars: its ultrastructure, development, and calcification.","authors":"T Yamamoto","doi":"10.1679/aohc.49.459","DOIUrl":"https://doi.org/10.1679/aohc.49.459","url":null,"abstract":"The present study describes the ultrastructure of the innermost layer of cementum (ILC) in the rat molars and discusses its developmental process and calcification mechanisms. The following points are the main results of this study: The ILC is a thin layer, about 2.0 micron thick, intensely stained with hematoxylin but not stained by silver impregnation. Electron microscopically, it is composed of substances stained with ruthenium red and chronic phosphotungstic acid presumed to be proteoglycans and a few thin collagen fibrils. Periodontal fibers penetrate the ILC only a short distance, and do not reach the root dentin surface. The ILC begins to form on the root dentin surface, just after the disintegration of Hertwig's epithelial root sheath. At the same time, matrix vesicles and spherical bodies, which may be derived from the matrix vesicles, appear on the surface of the developing ILC. Dental sac cells show higher cell activities than the epithelial sheath cells. Observations support the view that the dental sac cells secrete the ruthenium red positive material. On the basis of the above findings, the ILC is suggested to be formed by the dental sac cells and calcified by the matrix vesicles derived from these cells. The ILC can be regarded as a specialized cementum between the root dentin and the cementum in the strict sense, serving the connection of the two tissues.","PeriodicalId":8387,"journal":{"name":"Archivum histologicum Japonicum = Nihon soshikigaku kiroku","volume":"49 4","pages":"459-81"},"PeriodicalIF":0.0,"publicationDate":"1986-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.49.459","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14931463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Electron microscopic observation of the primary cilium in the pancreatic islets. 胰岛初级纤毛的电镜观察。

Archivum histologicum Japonicum = Nihon soshikigaku kiroku Pub Date : 1986-10-01 DOI: 10.1679/aohc.49.449

M Yamamoto, K Kataoka

{"title":"Electron microscopic observation of the primary cilium in the pancreatic islets.","authors":"M Yamamoto, K Kataoka","doi":"10.1679/aohc.49.449","DOIUrl":"https://doi.org/10.1679/aohc.49.449","url":null,"abstract":"A primary cilium often projected from the Golgi region of pancreatic A, B and D cells. The proximal portion of the cilium was found surrounded by a tubular invagination of the plasmalemma, and then the cilium extended into the intercellular canaliculus. The most proximal portion of the ciliary membrane exhibited periodical densities which might correspond to the ciliary necklace. The axoneme of the cilium was basically of the 9 + 0 pattern, i.e., nine peripheral doublets and no central singlet, though it was modified along the length of the cilium. Although a few appendages were projected from each doublet, it was difficult to identify dynein arms and nexin links. At the most proximal portion of the cilium, a \"champagne-glass\" structure connected each doublet with the ciliary membrane. The distal and proximal centrioles of the diplosome were connected to each other by a striated band. The proximal centriole, which served as a basal body, had accessory structures, such as alar sheets, basal feet and rootlets. Frequent projections of the primary cilium and its elaborate structure suggest that the cilium is not an aberrant structure but rather one which plays a certain role in the islet cell function.","PeriodicalId":8387,"journal":{"name":"Archivum histologicum Japonicum = Nihon soshikigaku kiroku","volume":"49 4","pages":"449-57"},"PeriodicalIF":0.0,"publicationDate":"1986-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.49.449","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14667011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Are the islets of Langerhans neuro-paraneuronal control centers of the exocrine pancreas? 朗格汉斯胰岛是外分泌胰腺的神经副神经元控制中心吗?

Archivum histologicum Japonicum = Nihon soshikigaku kiroku Pub Date : 1986-10-01 DOI: 10.1679/aohc.49.411

R Radke, W Stach

引用次数: 14

The structure of the pigment cells in the turtle Trionyx sinensis. 鳖色素细胞的结构。

Archivum histologicum Japonicum = Nihon soshikigaku kiroku Pub Date : 1986-10-01 DOI: 10.1679/aohc.49.421

P Gopalakrishnakone

引用次数: 0