Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB)最新文献_第7页

Changes in the retinal transition dipole moment in bacteriorhodopsin of the purple membrane of Halobacterium Salinarium at the so-called PH(rev). PH(rev)下盐杆菌紫膜细菌视紫红质视网膜过渡偶极矩的变化。

Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) Pub Date : 2002-02-01 DOI: 10.1080/10258140290010205

H. Mostafa

引用次数: 1

Protective effects of S-allylcysteine on hepatic glutathione and glutathione-dependent enzymes during hamster cheek pouch carcinogenesis. s -丙氨酸半胱氨酸对仓鼠颊袋癌变过程中肝谷胱甘肽和谷胱甘肽依赖酶的保护作用。

Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) Pub Date : 2002-02-01 DOI: 10.1080/10258140290010160

S. Balasenthil, S. Nagini

引用次数: 13

Existence of two emm-like "mrp" and "emm" genes in the mga regulon of the Streptococcus pyogenes strain ST4547. 化脓性链球菌ST4547菌株mga调控中存在两个类似emm的“mrp”和“emm”基因。

Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) Pub Date : 2002-02-01 DOI: 10.1080/10258140290010188

M. Eshaghi, A. Ali, F. Jamal, K. Yusoff

{"title":"Existence of two emm-like \"mrp\" and \"emm\" genes in the mga regulon of the Streptococcus pyogenes strain ST4547.","authors":"M. Eshaghi, A. Ali, F. Jamal, K. Yusoff","doi":"10.1080/10258140290010188","DOIUrl":"https://doi.org/10.1080/10258140290010188","url":null,"abstract":"Streptococcus pyogenes ST4547 is an opacity factor negative strain, which has been recently reported as a new emm type from Malaysia. Nucleotide sequencing of the mga regulon of this strain showed the existence of two emm-like genes. The emm gene located upstream of the scpA gene comprises 1305 nucleotides encoding the putative precursor M protein of 435 amino acids in length with an M(r) of 49 kDa. or a predicted mature protein of 394 amino acids with an M(r) of 44.8 kDa. Another gene mrpST4547 was located upstream of the emm gene and downstream of the mga gene. The sequence of this mrp gene comprises 1167 nucleotides encoding a predicted protein of 388 amino acids in length with an M(r) of 42.2 kDa. or a predicted mature protein of 347 amino acids with an M(r) of 37.9 kDa. The mga regulon of strain ST4547 has a mosaic structure comprising segments, which originated from different OF positive and OF negative strains. The sequences flanking the hyper-variable and C repeats of the emmST4547 gene showed high similarity to corresponding regions in the mga regulon of OF positive strains notably M15, M4, M22 and M50. In contrast, the sequence within the hyper-variable and C repeat regions of the emmST4547 gene revealed high similarity to equivalent regions in the OF negative strains. These data indicates that horizontal transfer of emm-like gene could have occurred between OF positive and OF negative strains resulting in architectural divergence in the mga regulon.","PeriodicalId":83764,"journal":{"name":"Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB)","volume":"35 1","pages":"23-8"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85127589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The omega-hydroxy palmitic acid induced apoptosis in human lung carcinoma cell lines H596 and A549. omega-羟基棕榈酸诱导人肺癌细胞株H596和A549凋亡。

Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) Pub Date : 2002-02-01 DOI: 10.1080/10258140290010223

A. Abe, M. Yamane, H. Yamada, I. Sugawara

{"title":"The omega-hydroxy palmitic acid induced apoptosis in human lung carcinoma cell lines H596 and A549.","authors":"A. Abe, M. Yamane, H. Yamada, I. Sugawara","doi":"10.1080/10258140290010223","DOIUrl":"https://doi.org/10.1080/10258140290010223","url":null,"abstract":"We have found that omega-hydroxy palmitic acid (16-hydroxy palmitic acid, omega-HPA) has both cell growth inhibiting and cell death inducing actions on human lung adenosquamous carcinoma cell line H596 and adenocarcinoma cell line A549. Further, these effects were dose- and time-dependent in both cell lines. However, in squamous carcinoma cell line H226, omega-HPA had no cytotoxic effect. On the other hand, in the human small cell lung carcinoma (SCLC) cell line H128, this compound showed weak cytotoxicity. The sensitivity toward omega-HPA was higher in H596 cells than in A549 cells. In both H596 and A549 cells, cell growth was inhibited to 24.4 and 9.4%, respectively, by treatment with 100 microM omega-HPA for 12 h. In the 24 h treatment cells, growth inhibition was increased to 100 and 38.1%, respectively. In cytotoxicity experiments, the number of dead cells increased with incubation times in the presence of omega-HPA: on three days incubation with 100 microM omega-HPA, viability was 0 and 13.5%, respectively, in H596 and A549 cells. Further, the fragmentation of DNA to oligonucleosomal-sized ladder fragments, which is an index of apoptosis, was observed in both cell lines on treatment with omega-HPA. Therefore, it is assumed that these cell deaths induced by omega-HPA, were apoptosis in these cell lines. Since the number of dead cells following treatment with omega-HPA decreased by treatment with omega-HPA in combination with Z-VAD-fmk, a caspase family inhibitor, it is thought that apoptotic cell death was related to caspase activity.","PeriodicalId":83764,"journal":{"name":"Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB)","volume":"55 1","pages":"37-43"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79841572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Enriched levels of erythropoietin in human umbilical cord blood stimulate hematopoietic progenitor cells. 人脐带血中促红细胞生成素的丰富水平刺激造血祖细胞。

Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) Pub Date : 2002-02-01 DOI: 10.1080/10258140290010214

G. Lawlor, T. Tauchi, K. Ohyashiki, T. Matsumiya

{"title":"Enriched levels of erythropoietin in human umbilical cord blood stimulate hematopoietic progenitor cells.","authors":"G. Lawlor, T. Tauchi, K. Ohyashiki, T. Matsumiya","doi":"10.1080/10258140290010214","DOIUrl":"https://doi.org/10.1080/10258140290010214","url":null,"abstract":"Human umbilical cord blood (CB) is a recognized source of hematopoietic tissues for transplantation, the treatment of malignancies and gene therapy, among other potential clinical applications. A rich network of hematopoietic cytokines and growth factors possessing stimulatory effects on primitive hematopoietic stem/progenitor cells further characterizes fetal CB. To better elucidate these complex interactions and properties, we compared the hematopoietic activities of CB and normal human peripheral blood (PB), by examining growth/survival of normal hematopoietic progenitors and erythropoietin-dependent UT-7/EPO cells. Colony-forming activity assays of normal bone marrow (BM) BFU-E and CFU-GM showed that CB significantly enhanced progenitor cell growth in comparison to PB. Apoptosis was determined by enumerating APO 2.7 mAb stained cells using flow cytometry. UT-7/EPO cell cultures subjected to PB exhibited a four-fold higher rate of apoptosis than CB exposed cultures, indicating that CB markedly suppressed apoptosis in this human leukemic cell line. Immunoprecipitation of UT-7/EPO cell lysates and immunodetection of the anti-phosphotyrosine Ab (4G10), revealed that CB induced tyrosine phosphorylation of three proteins, with approximate molecular masses of 160, 117.5 and 80 kDa. The 80 kDa protein corresponds to the previously reported molecular mass for the Epo receptor, suggesting that erythropoietin is enriched in CB compared with adult PB.","PeriodicalId":83764,"journal":{"name":"Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB)","volume":"42 1","pages":"65-70"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88853335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Kinetics of inhibition and molecular asymmetry in pigeonpea (Cajanus cajan) urease. 鸽豆脲酶抑制动力学及分子不对称性研究。

Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) Pub Date : 2002-02-01 DOI: 10.1080/10258140290010142

P. Srivastava, A. Kayastha, M. Jagannadham

{"title":"Kinetics of inhibition and molecular asymmetry in pigeonpea (Cajanus cajan) urease.","authors":"P. Srivastava, A. Kayastha, M. Jagannadham","doi":"10.1080/10258140290010142","DOIUrl":"https://doi.org/10.1080/10258140290010142","url":null,"abstract":"Urease from seeds of pigeonpea showed a time-dependent and irreversible inactivation at very low concentrations of heavy metal ions. Concentration of Cu(2+), Hg(2+) and Ag(+) required for 50% inactivation, on 10 min of incubation, were found to be 2.2 x 10(-6), 2.9 x 10(-8) and 6.3 x 10(-12) M, respectively. The kinetics of inactivation with each of these metal ions was found to be biphasic, with half of the activity being lost in a fast phase and remaining in a slow phase. Acetohydroxamate (AHA) inhibits pigeonpea urease competitively and reversibly with a K(i) of 0.041 mM at pH 7.3. This inhibition was found to be pH dependent. A reversible and time-dependent inhibition was observed with AHA. AHA inhibition revealed biphasic kinetics as observed with the heavy metal ions. Pigeonpea urease was also inhibited by fluoride ions competitively with a K(i) value of 1.23 mM. These inhibition studies suggest the possible interaction of these inhibitors with active site thiol groups and Ni (II) ion. A mechanism has been proposed for each of these inhibitors and compared with inhibition studies reported for other ureases.","PeriodicalId":83764,"journal":{"name":"Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB)","volume":"18 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72935310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Phage display of recombinant antibodies toward Burkholderia pseudomallei exotoxin. 假氏伯克氏菌外毒素重组抗体的噬菌体展示。

Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) Pub Date : 2002-02-01 DOI: 10.1080/10258140290010232

S. Nathan, Hongbin Li, R. Mohamed, N. Embi

{"title":"Phage display of recombinant antibodies toward Burkholderia pseudomallei exotoxin.","authors":"S. Nathan, Hongbin Li, R. Mohamed, N. Embi","doi":"10.1080/10258140290010232","DOIUrl":"https://doi.org/10.1080/10258140290010232","url":null,"abstract":"We have used the phagemid pComb3H to construct recombinant phages displaying the single chain variable fragment (ScFv) towards exotoxin of Burkholderia pseudomallei. Variable heavy and light chain fragments were amplified from the hybridoma 6E6A8F3B line, with a wide spectrum of primers specific to mouse antibody genes. Through overlapping extension polymerase chain reaction, the heavy and light chain fragments were linked to form the ScFv which was subsequently cloned into the phage display vector and transformed into ER2537 cells to yield a complexity of 10(8) clones. The transformants were screened by four rounds of biopanning against the exotoxin and resulted in selective enrichment of exotoxin-binding antibodies by 301 fold. The phage pool from the final round of selection displayed antibodies of high-affinity to the exotoxin as demonstrated by ELISA. Several clones were selected randomly from this pool and analysed by restriction enzyme digestion, fingerprinting and sequencing. Restriction analysis confirmed that all clones carried a 700-800 bp insert whose sequences, in general, corresponded to that of mouse IgG. Fingerprinting profiles delineated the antibodies into two families with different CDR sequences.","PeriodicalId":83764,"journal":{"name":"Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB)","volume":"142 1","pages":"45-53"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80171804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Possible involvement of dopaminergic neurotransmitter system in dichlorvos induced delayed neurotoxicity. 多巴胺能神经递质系统可能参与敌敌畏诱导的迟发性神经毒性。

Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) Pub Date : 2002-02-01 DOI: 10.1080/10258140290010197

S. Choudhary, Geetu Raheja, Vandana Gupta, K. Gill

{"title":"Possible involvement of dopaminergic neurotransmitter system in dichlorvos induced delayed neurotoxicity.","authors":"S. Choudhary, Geetu Raheja, Vandana Gupta, K. Gill","doi":"10.1080/10258140290010197","DOIUrl":"https://doi.org/10.1080/10258140290010197","url":null,"abstract":"The present investigation was carried out to elucidate the possible involvement of the dopaminergic neurotransmitter system in the development of dichlorvos induced delayed neurotoxicity in the rat and to assess the protective efficacy of nimodipine against OPIDN (Organophosphate induced delayed neurotoxicity). Single subcutaneous dose of dichlorvos (200 mg/kg body weight) resulted in marked changes in the dopaminergic neurotransmitter system in terms of increased levels of both dopamine and norepinephrine along with significant increase in the activity of both the catecholamine synthesizing enzymes, tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase. This increase was accompanied with a concomitant decrease in the activity of the major degradative enzyme, monoamine oxidase. Scatchard plot analysis revealed a significant decrease in both K(d) and B(max) for dopamine D2 receptors. Administration of nimodipine, a centrally acting calcium channel blocker, along with dichlorvos restricted all these alterations to within control values and could also ameliorate certain behavioural deficits by maintaining the dopaminergic neurotransmitter system. The study underlines the importance of alterations in the dopamine system as a possible causative mechanism behind the behavioural and functional changes associated with delayed neurotoxicity.","PeriodicalId":83764,"journal":{"name":"Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB)","volume":"75 1","pages":"29-36"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77081864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum. 鸡盲肠乳酸乳球菌耐红霉素质粒的鉴定与分子克隆。

Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) Pub Date : 2002-02-01 DOI: 10.1080/10258140290010151

A. Raha, E. Ross, K. Yusoff, M. Y. Manap, A. Ideris

引用次数: 11

Mitochondrial factors modulate the activity of endonuclease G, the major nuclease of Mammalian mitochondria. 线粒体因子调节哺乳动物线粒体的主要核酸酶内切酶G的活性。

Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) Pub Date : 2002-02-01 DOI: 10.1080/10258140290010179

S. Ikeda, Yuichi Seki, K. Ozaki

{"title":"Mitochondrial factors modulate the activity of endonuclease G, the major nuclease of Mammalian mitochondria.","authors":"S. Ikeda, Yuichi Seki, K. Ozaki","doi":"10.1080/10258140290010179","DOIUrl":"https://doi.org/10.1080/10258140290010179","url":null,"abstract":"Endonuclease G (Endo G), a sugar-nonspecific nuclease preferring single-stranded DNA (ssDNA), is responsible for major nuclease activity in mitochondria. If the enzyme provides an important nicking function for mitochondrial DNA (mtDNA) in vivo, then mitochondrial factors likely exist which modulate the enzyme's activity and prevent cleavage at single-stranded moieties of mtDNA. In the present paper, we report that specific membrane phospholipids, polyamines and single-stranded DNA-binding protein (SSB) appear to exert such effects in vitro. Phosphatidylcholine and phosphatidylethanolamine, the major constituents of the mitochondrial inner membrane, stimulated purified Endo G activity 5- to 10-fold. Spermine at 5-100 microM also stimulated activity about 4-fold. However, at more than 500 microM, the spermine largely inhibited the degradation of ssDNA and duplex DNA. Escherichia coli SSB, which has physicochemical properties analogous to those of mitochondrial SSB (mtSSB), markedly inhibited the degradation of phiX174 ssDNA by Endo G, indicating the possible involvement of mtSSB in vivo in protection of single-stranded regions of mtDNA from nucleolytic attacks.","PeriodicalId":83764,"journal":{"name":"Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB)","volume":"16 1","pages":"17-21"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82165554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4