Pediatric pathology & molecular medicine最新文献_第4页

Case report: congenital salivary gland analage tumor presenting with neonatal respiratory distress. 一例报告:以新生儿呼吸窘迫为表现的先天性唾液腺瘘瘤。

Pediatric pathology & molecular medicine Pub Date : 2003-05-01

Salah Ahmed Ibrahim, Haydar Abubakr Abdalla, Ahmed Mohammed El Hassan

引用次数: 0

Familial hemophagocytic lymphohistiocytosis: an autopsy study. 家族性噬血细胞淋巴组织细胞病:尸检研究。

Pediatric pathology & molecular medicine Pub Date : 2003-05-01 DOI: 10.1080/pdp.22.3.229.242

Nandita Kakkar, R K Vasishta, A K Banerjee, R K Marwaha, B R Thapa

引用次数: 11

Detection of n-myc gene amplification in neuroblastoma by comparative, in situ,and real-time polymerase chain reaction. 通过比较、原位和实时聚合酶链反应检测神经母细胞瘤中n-myc基因扩增。

Pediatric pathology & molecular medicine Pub Date : 2003-05-01 DOI: 10.1080/15227950307737

Z. Melegh, I. Bálint, E. Tóth, E. Csernák, Z. Szentirmay, Kálmán Nagy

{"title":"Detection of n-myc gene amplification in neuroblastoma by comparative, in situ,and real-time polymerase chain reaction.","authors":"Z. Melegh, I. Bálint, E. Tóth, E. Csernák, Z. Szentirmay, Kálmán Nagy","doi":"10.1080/15227950307737","DOIUrl":"https://doi.org/10.1080/15227950307737","url":null,"abstract":"We have used semiquantitative comparative and real-time quantitative polymerase chain reactions (PCR) to detect n-myc gene-amplification in 20 frozen neuroblastoma biopsies and IMR 32 cell line to predict biological behavior of the tumors. Two primer pairs were used for the semiquantitative method to co-amplify a 520-bp fragment of the beta-globin gene--used as a single copy reference standard--and a 258-bp fragment of the n-myc gene. After 30 cycles the PCR products were electrophoresed through an agarose gel and were compared to each other with use of a gel-densitometer. Real-time quantitative analyses were performed in a LightCycler instrument. A single primer pair was used to amplify a 120-bp fragment of the n-myc oncogene and a LC640-labeled fluorescent probe pair to detect the product. Calibration curve, set up from a serial dilution including samples with 1, 2, 10, 13, 25-fold n-myc oncogene amplification, was used for quantitative analysis. The semiquantitative method did not show distinct difference between tumor groups with no amplification and less than 10-fold amplification, whereas quantitative LightCycler analysis was able to detect even 2-fold amplification. Differentiated neuroblastomas seldom show n-myc amplification. In spite of this, we have found two partly differentiated tumor samples that contained n-myc amplification. In these cases in situ PCRs were performed to examine the tumor heterogeneity. We used biotinated ATP labeling and the same primer pair as for the LightCycler analysis. In both cases differentiated cells did not show n-myc gene amplification, whereas considerable amplification was detected in the neuroblasts.","PeriodicalId":82276,"journal":{"name":"Pediatric pathology & molecular medicine","volume":"22 3 1","pages":"213-22"},"PeriodicalIF":0.0,"publicationDate":"2003-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59824857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Placental calcium pump: clinical-based evidence. 胎盘钙泵:临床证据。

Pediatric pathology & molecular medicine Pub Date : 2003-05-01 DOI: 10.1080/pdp.22.3.223.227

John M Kasznica, Eugen B Petcu

引用次数: 9

Pathology teach and tell: intravascular endothelial hyperplasia forming colonic polyps. 病理提示:血管内内皮增生形成结肠息肉。

Pediatric pathology & molecular medicine Pub Date : 2003-03-01 DOI: 10.1080/pdp.22.2.181.185

Nandita Kakkar, R K Vasishta, K L Narsimhan

{"title":"Pathology teach and tell: intravascular endothelial hyperplasia forming colonic polyps.","authors":"Nandita Kakkar, R K Vasishta, K L Narsimhan","doi":"10.1080/pdp.22.2.181.185","DOIUrl":"https://doi.org/10.1080/pdp.22.2.181.185","url":null,"abstract":"A12-year-oldmale childwasbrought to the emergency roomwith fresh rectalbleeding for the preceeding 24 h.There was a similar episode 6 months earlier that had subsided spontaneously. On examination, the child was pale, restless, and the peripheral pulses were thready. His blood pressure was 60=40 mmHg in the right upper limb; hemoglobin was 6.6 mmol=l, platelet count and coagulation pro¢le were normal.The child was resuscitated with fresh whole blood and explored.The entire colon up to the ileocecal junctionwas ¢lled with fresh blood. On careful palpation of the colon, a nodular circumscribed ¢rmmass,0.5 cm in diameter, was felt arising in the transverse colon near the splenic £exure. The segment of the colon containing the mass was resected and colon continuity was restored by end-to-end anastomosis. Gross examination of the colon showed small sessile polypoidal projections ranging in size from 0.3 to 0.6 cm in diameter. Few of the polyps were hemorrhagic. On microscopy, the vessels in the mucosa and submucosa revealed a variety of lesions. Many vessels showed an exhuberant solid intravascular endothelial proliferation forming tumor nodules within the vessels with total occlusion of their lumina (F|gure1).These intravascular nodules projected into the lumenof thebowel as polyps. The proliferating cells were plump endothelial cells with small lumina that did not showanyatypia ormitosis (F|gure 2).The endothelial nature of the cells was con¢rmed by a strong positive immunostaining for factor VIII^ related antigen. Some of the vessels were lined by large prominent endothelial cells only and few revealed multilayering with slight luminal compromise (F|gure 3). Few of the polyps containing vessels with intravascular endothelial proliferation revealed ulceration with active bleeding (F|gure 4). Only an occasional vessel showed endothelial proliferation admixed with ¢brin (F|gure 4).","PeriodicalId":82276,"journal":{"name":"Pediatric pathology & molecular medicine","volume":"22 2","pages":"181-5"},"PeriodicalIF":0.0,"publicationDate":"2003-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/pdp.22.2.181.185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22219005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The isoprenoid pathway and the pathogenesis of Reye's syndrome. 类异戊二烯途径与雷氏综合征的发病机制。

Pediatric pathology & molecular medicine Pub Date : 2003-03-01

Ravi Kumar Kurup, Parameswara Achutha Kurup

{"title":"The isoprenoid pathway and the pathogenesis of Reye's syndrome.","authors":"Ravi Kumar Kurup, Parameswara Achutha Kurup","doi":"","DOIUrl":"","url":null,"abstract":"Unlabelled: The isoprenoid pathway produces three key metabolites: endogenous digoxin (regulator of neurotransmitter uptake), dolichol, and ubiquinone (free radical scavenger). Because a mitochondrial dysfunction has been described in Reye's syndrome, we thought it pertinent to assess the pathway in this disease. Since endogenous digoxin can regulate neurotransmitter transport, the pathway also was assessed in patients with right hemispheric, left hemispheric, and bihemispheric dominance to find out the role of hemispheric dominance in its pathogenesis. The plasma/serum activity of HMG CoA reductase, magnesium, digoxin, dolichol, ubiquinone, tryptophan/tyrosine catabolic patterns, free radical, and lipid levels as well as (red blood cell) RBC Na(+)-K(+) ATPase activity were measured in the above mentioned groups.Results: In the patient group as well as in individuals with right hemispheric dominance similar patterns were obtained. There was elevated digoxin and dolichol levels with low levels of ubiquinone in patients with Reye's syndrome as well as in those with right hemispheric dominance. The serum magnesium and RBC Na(+)-K(+) ATPase activity were reduced. There also was an increase in tryptophan catabolites and a reduction in tyrosine catabolites as well as increased free radical levels. Reye's syndrome is associated with an upregulated isoprenoid pathway, elevated hypothalamic digoxin secretion, and right hemispheric chemical dominance.","PeriodicalId":82276,"journal":{"name":"Pediatric pathology & molecular medicine","volume":"22 2","pages":"159-70"},"PeriodicalIF":0.0,"publicationDate":"2003-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22219002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnosis of congenital nephrotic syndrome: a clinical and a pathologic challenge. 先天性肾病综合征的诊断:一个临床和病理的挑战。

Pediatric pathology & molecular medicine Pub Date : 2003-03-01 DOI: 10.1080/pdp.22.2.105.116

Ada Rivera, Suzanne Meleg-Smith, Igor Yosipiv, Samir El-Dahr, Frank Boineau

{"title":"Diagnosis of congenital nephrotic syndrome: a clinical and a pathologic challenge.","authors":"Ada Rivera, Suzanne Meleg-Smith, Igor Yosipiv, Samir El-Dahr, Frank Boineau","doi":"10.1080/pdp.22.2.105.116","DOIUrl":"https://doi.org/10.1080/pdp.22.2.105.116","url":null,"abstract":"The diagnosis of congenital nephrotic syndrome (NS) is a challenge both for clinicians and for pathologists. We observed three cases in a series of 50 children with NS nonresponsive to therapy, corresponding to one case each of minimal change disease, Finnish-type glomerulopathy, and diffuse mesangial sclerosis--two histopathologic studies were performed in each case. The age at presentation did not predict the diagnosis nor the prognosis: The NS presented at 7 months of age in the patient with diffuse mesangial sclerosis, but it was present at birth in the patient with minimal change disease. In these 2 patients the final diagnosis was made with the first renal biopsy. Conversely, in the patient with Finnish-type glomerulopathy, the diagnosis was only possible in the repeat biopsy, as the early pathologic changes were nonspecific. This study shows the essential role of the renal biopsy in determining the etiologic diagnosis and prognosis in patients with congenital nephrotic syndrome. congenital nephrotic syndrome diffuse mesangial sclerosis Finnish-type glomerulopathy","PeriodicalId":82276,"journal":{"name":"Pediatric pathology & molecular medicine","volume":"22 2","pages":"105-16"},"PeriodicalIF":0.0,"publicationDate":"2003-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/pdp.22.2.105.116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22219659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Pathology teach and tell: melanotic ependymoma. 病理诊断:黑色室管膜瘤。

Pediatric pathology & molecular medicine Pub Date : 2003-03-01 DOI: 10.1080/pdp.22.2.171.174

Nandita Kakkar, R K Vasishta, A K Banerjee, Arun Bansal

引用次数: 3

Letter to the editor--multifocal myocardial apoptoses: distinctive cardiac lesions in cystic fibrosis and other diseases. 致编辑的信——多灶性心肌凋亡:囊性纤维化和其他疾病的独特心脏病变。

Pediatric pathology & molecular medicine Pub Date : 2003-03-01 DOI: 10.1080/pdp.22.2.187.188

Jiri T Beranek

{"title":"Letter to the editor--multifocal myocardial apoptoses: distinctive cardiac lesions in cystic fibrosis and other diseases.","authors":"Jiri T Beranek","doi":"10.1080/pdp.22.2.187.188","DOIUrl":"https://doi.org/10.1080/pdp.22.2.187.188","url":null,"abstract":"Being interested in the mechanism of cardiomyocyte cell death [1], I have read with attention the article by Nezelof et al. [in this publication; 2] about multifocal myocardial necrosis in cystic ¢brosis and other diseases.Today, it is known that necrosis, called more suitably accidental cell death, leads to in£ammatory reaction whereas apoptosis, the secondmechanismof cell death, does notprovoke in£ammation. Since there is no in£ammatory reaction in the cases described by Nezelof et al. [2], wemayask justi¢ably whether the cardiomyocytes in question have not died by apoptosis instead of necrosis. Indeed, cardiomyocyte apoptosis is present in F|gure 2 of the article [2].Numerous atrophic cardiomyocytes are scattered amid di¡use ¢brosis in the upper two thirds of the ¢gure. Amajority of themmanifest a fragmentation into small apoptotic bodies. The reader may be mislead by their similarity to vessels ¢lled with red cells [1], a phenomenon already described in the hearts of systemic sclerosis patients [3]. Acarefulmorphologic analysis shows, however, that apoptoticbodies are inside cardiomyocytes and cannot be, therefore, red cells. In the bottom third of F|gure 2, cardiomyocytes are less damaged and it is easy to ¢nda clearly recognizable myo¢ber fragmenting into apoptoticbodies [2].The lackof in£ammatory reaction in F|gure 2 also con¢rms the conclusion that the cardiomyocytes in question are dying by apoptosis [2]. F|gures1, 3, and 4 are, unfortunately, of too lowmagni¢cation tovisualize cellular details [2].They show, however, numerousmyocardial clefts that are not present in the normal heart and that often contain tissue remains.The latter may be either artefacts (the shrunken interstitium) or deadcardiomyocytes. I would advance that the second alternative is true because the tissue remains often manifest similar tinctorial properties as surrounding them, cardiomyocytes and=or contain dark small apoptototic bodies inside them. Again, the lack of in£ammatory reaction indicates","PeriodicalId":82276,"journal":{"name":"Pediatric pathology & molecular medicine","volume":"22 2","pages":"187-8"},"PeriodicalIF":0.0,"publicationDate":"2003-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/pdp.22.2.187.188","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22217751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathology teach and tell: neonatal ELS. 病理教与讲:新生儿ELS。

Pediatric pathology & molecular medicine Pub Date : 2003-03-01 DOI: 10.1080/pdp.22.2.175.179

Pratibha A Ankola, Ashraf Morkos

{"title":"Pathology teach and tell: neonatal ELS.","authors":"Pratibha A Ankola, Ashraf Morkos","doi":"10.1080/pdp.22.2.175.179","DOIUrl":"https://doi.org/10.1080/pdp.22.2.175.179","url":null,"abstract":"A2790-gs full-termmalebornby normal spontaneous vaginal delivery to a 28year-old gravida 2 para1with antenatal fetal diagnosis of cystic left lungmass associated with mediastinal shift to the right and no hydrops fetalis. The lung mass was ¢rst seen at 20 weeks gestation on ultrasound and increased in size over 9 weeks. Follow-up ultrasound at 34 weeks showed decrease in size of the mass. Karytotypewas 46XY. Apgar scores were 9 and 9 at1and 5 min, respectively. Physical examination of the infant at birth revealed tachypneawith respiratory rate 80=min min and mild lower intercostal retractions. Air entry was equal and good bilaterally. Heart soundswere heardbest to the right of themidline.Oxygen saturation remained 96^99%. Initial blood gas was within normal limits. Baby was placed on nasal CPAP 4 with F|02 0.25 due to increasing tachypnea anddesaturation. ChestX-ray showed left lower lobe density, withmediastinal shift to the right. Left diaphragmwasvisualizedwell (F|gure1).Computedtomography (CT) scanwith POcontrast con¢rmedthe cystic left lungmass (F|gure 2). Di¡erential diagnosis included bronchopulmonary sequestration and congenital cystic adenomatoid (CCAM) malformation of the lung.The baby had increased pCO2 with tachypnea needing intubation and mechanical ventilation from the 5th day. Echocardiogram showed tiny patent ductus arteriosus (PDA), large left atrial appendage, and a large £ow in left pulmonary veins.Thebabyhadelective surgeryonthe11th day. At surgery, amassmeasuring 5 4 cmwas removedandcon¢rmedtobe a left extralobar sequestrationwithblood supply fromavessel arising fromthe aorta.Thebabywas extubated uneventfully on the 3rd postoperative day and placed on nasal CPAP for1day before weaning o¡ to room air.The infant remained tachypneic intermittently with respiratory rate up to 80=min for 2 days.The baby was discharged on the10th postoperative day in stable condition.Histopathologyof themass con¢rmedthe diagnosis (F|gure 3).","PeriodicalId":82276,"journal":{"name":"Pediatric pathology & molecular medicine","volume":"22 2","pages":"175-9"},"PeriodicalIF":0.0,"publicationDate":"2003-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/pdp.22.2.175.179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22217752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4