Annals of vascular surgery最新文献

{"title":"Endovascular aneurysm repair with Zenith Alpha Abdominal stent-graft in hostile and non-hostile aortic neck anatomies.","authors":"Giulia Bertagna, Nicola Troisi, Raffaele Pulli, Gianmarco de Donato, Sofia Pierozzi, Valerio Artini, Raffaella Berchiolli","doi":"10.1016/j.avsg.2025.03.011","DOIUrl":"https://doi.org/10.1016/j.avsg.2025.03.011","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to evaluate early and 5-year outcomes of EndoVascular Aortic Repair (EVAR) with Zenith Alpha Abdominal in the context of a multicenter regional retrospective registry comparing patients with hostile and non-hostile aortic necks.</p><p><strong>Methods: </strong>A retrospectively maintained dataset identified all consecutive patients with Abdominal Aortic Aneurysms (AAAs) underwent elective EVAR with implantation of a Zenith Alpha Abdominal in 7 centers between January 2016 and December 2022. Two-hundred-twenty-eight patients have been included in the present study: 98 (43%) with a hostile neck (Group HN), and 130 (57%) with a no-hostile neck (Group n-HN). Early (30-day) outcomes in terms of technical and clinical successes were assessed and compared. Estimated 5-year outcomes were evaluated and compared in terms of survival, freedom from type I/III endoleak, freedom from surgical conversion, freedom from limb graft occlusion, and freedom from any device-related reintervention(s) by using life-table analysis (Kaplan-Meier curves) and log-rank test.</p><p><strong>Results: </strong>Female gender was more frequent in Group HN (15.3% vs. 5.3%, p=0.01). Thirty-day technical success rate was 96.9% in Group HN, and 100% in Group n-HN (p=0.08), whilst 30-day clinical success rate was 96.9% in Group HN, and 99.2% in Group n-HN (p=0.21). Overall median follow-up period was 32.1 months [InterQuartile Range (IQR) 14-47]. Estimated 5-year survival rates were comparable (67.1% in Group HN, and 77.9% in Group n-HN, p=0.47). During the follow-up no endoleak type III have been detected in both groups. At 5 years there were no differences between the two groups in terms of freedom from surgical conversion (95.1% Group HN vs. 96.7% Group n-HN; p=0.71, log-rank 0.14), freedom from limb graft occlusion (95.7% Group HN vs. 93.5% Group n-HN; p=0.58, log-rank 0.29), and freedom from any device-related reintervention(s) (70.5% Group HN vs. 89.7% Group n-HN; p=0.19, log-rank 1.72). Starting from the third year of follow-up, hostile neck affected type I endoleak rate (70.2% Group HN vs. 98.4% Group n-HN; p=0.008, log-rank 6.96). Female gender was the only factor affecting type I endoleak onset during the follow-up (p=0.02; log-rank=5.44) CONCLUSIONS: In the present multicenter experience hostile neck was more frequent in female patients. Hostile neck affected type I endoleak rates in patients undergoing Zenith Alpha Abdominal implantation starting from the third year of follow-up from the index procedure.</p>","PeriodicalId":8061,"journal":{"name":"Annals of vascular surgery","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of venous outflow and venous capacity in predicting postthrombotic syndrome following pharmacomechanical thrombolysis for acute iliofemoral vein thrombosis.","authors":"Demir Cetintas, Hakan Guven","doi":"10.1016/j.avsg.2025.03.006","DOIUrl":"https://doi.org/10.1016/j.avsg.2025.03.006","url":null,"abstract":"<p><strong>Background: </strong>We aimed to evaluate the impact of hemodynamic parameters in predicting the development of post-thrombotic syndrome (PTS) following acute iliofemoral deep vein thrombosis (DVT) after pharmacomechanical thrombolysis.</p><p><strong>Methods: </strong>A total of 59 patients with acute deep vein thrombosis undergoing pharmacomechanical thrombolysis were included in this retrospective case-control study, and divided into two groups according to the development of postthrombotic syndrome; as postthrombotic syndrome group (n=18) and non-postthrombotic syndrome group (n=41). The groups were compared in terms of basic demographic and clinical characteristics, as well as clinical and hemodynamic assessment scores, including venous capacity and outflow measured using strain-gauge venous occlusion plethysmography.</p><p><strong>Results: </strong>There were no significant differences between the groups in terms of basic demographic and clinical characteristics. The groups were statistically similar in terms of preprocedural clinical and hemodynamic assessment scores. However, compared to nonpostthrombotic syndrome group, in postthrombotic syndrome group the mean values of venous outflow were significantly lower whereas the mean values of venous capacity were significantly higher in postprocedural follows at 6. and 24. months.</p><p><strong>Conclusions: </strong>The present study demonstrated the critical role of venous outflow and venous capacity for predicting the development of postthrombotic syndrome in patients with acute iliofemoral vein thrombosis undergoing pharmacomechanical thrombolysis.</p>","PeriodicalId":8061,"journal":{"name":"Annals of vascular surgery","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-Term Outcomes of Open Surgical Repair for Complex Aortoiliac Occlusive Disease in the Age of Endovascular Advancements","authors":"V. Makaloski ,&nbsp;L. Ilcheva ,&nbsp;S. Valcheva-Ilieva ,&nbsp;M. Darwish ,&nbsp;D. Kotelis ,&nbsp;T.R. Wyss","doi":"10.1016/j.avsg.2025.03.007","DOIUrl":"10.1016/j.avsg.2025.03.007","url":null,"abstract":"<div><h3>Background</h3><div>Aortoiliac occlusive disease (AIOD) may lead to critical limb ischemia, requiring revascularization. Open repair remains preferred for complex type C and D lesions despite endovascular options. This study evaluated long-term outcomes of open surgical repair in acute and chronic AIOD.</div></div><div><h3>Methods</h3><div>A single-centre, retrospective analysis was conducted on patients undergoing open AIOD repair (January 2010-December 2020). Primary outcomes included 30-day and long-term mortality; secondary outcomes were graft patency, Rutherford classification improvement, and limb salvage.</div></div><div><h3>Results</h3><div>Seventy-six patients (61% male; mean age 62 ± 10 years) underwent open repair for Trans-Atlantic Inter-Society Consensus (TASC) II type D AIOD. Peripheral arterial disease and prior interventions were common (67 patients, 88%). Elective surgery was performed in 49 patients (64%), while 27 patients (36%) underwent urgent procedures. The 30-day mortality rate was 0% (0/49) for elective cases but 19% (5/27) for urgent cases (<em>P</em> &lt; 0.01). Long-term survival rates at 1, 2, 3, and 5 years were 98% (48/49), 96% (47/49), 94% (46/49), and 86% (42/49) for elective cases versus 78% (21/27), 62% (17/27), 62% (17/27), and 62% (17/27) for urgent cases (<em>P</em> = 0.30), respectively. Elective cases demonstrated high graft patency at 1, 2, 3, and 5 years (96% [47/49], 90% [44/49], 90% [44/49], and 85% [42/49]), respectively. Rutherford classification improved significantly (median preoperative stage 3 to postoperative stage 0, <em>P</em> &lt; 0.001), with stable ankle-brachial index (ABI) values (≥0.9) in 59 of 61 patients (97%). Vascular complications occurred in 39 patients (51%), with reinterventions in 12 patients (16%). Sustained limb salvage was observed over long-term follow-up (median 59 months).</div></div><div><h3>Conclusion</h3><div>Open repair for complex AIOD offers durable outcomes, with low elective case mortality and high limb salvage and graft patency. Future studies should explore long-term comparative outcomes.</div></div>","PeriodicalId":8061,"journal":{"name":"Annals of vascular surgery","volume":"116 ","pages":"Pages 9-16"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to Characterization of Strokes following Gore Thoracic Branch Endografting Requiring Zone 2 Landing [Ann Vasc Surg 2025; 110(Pt A): 447–459]","authors":"Grace J. Wang ,&nbsp;Nimesh D. Desai ,&nbsp;Joseph E. Bavaria ,&nbsp;Scott E. Kasner ,&nbsp;Himanshu J. Patel ,&nbsp;Michael D. Dake","doi":"10.1016/j.avsg.2025.03.002","DOIUrl":"10.1016/j.avsg.2025.03.002","url":null,"abstract":"","PeriodicalId":8061,"journal":{"name":"Annals of vascular surgery","volume":"114 ","pages":"Page 182"},"PeriodicalIF":1.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Questioning the Predictive Utility of the Novel Preoperative Risk Score for Nonhome Discharge after Open Abdominal Aortic Aneurysm Repair","authors":"Muhammad Khubaib Iftikhar,&nbsp;Qurat ul ain Iftikhar","doi":"10.1016/j.avsg.2025.03.003","DOIUrl":"10.1016/j.avsg.2025.03.003","url":null,"abstract":"","PeriodicalId":8061,"journal":{"name":"Annals of vascular surgery","volume":"115 ","pages":"Pages 225-226"},"PeriodicalIF":1.4,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Efficacy of Dual-layer Stent Compared to Single-layer Stent in Carotid Revascularization: A Systematic Review and Meta-analysis","authors":"Jianfeng Gao,&nbsp;Yida Cao,&nbsp;Yaoguo Yang,&nbsp;Shuo Wang,&nbsp;Huanqin Zheng,&nbsp;Zhong Chen","doi":"10.1016/j.avsg.2025.02.031","DOIUrl":"10.1016/j.avsg.2025.02.031","url":null,"abstract":"<div><h3>Background</h3><div>Prior studies have demonstrated the dual-layer stent (DLS) was associated with encouraging results in carotid revascularization. This meta-analysis aimed to study the comparative efficacy between DLS and single-layer stent (SLS).</div></div><div><h3>Methods</h3><div>The studies were retrieved from PubMed, Embase, and Scopus up to June 2023. The methodological evaluation was performed using the corresponding scale. Pooled analysis was conducted using R Studio to calculate the effects, including odds ratio (OR) and mean difference (MD). Heterogeneity among results was assessed using the I² statistic. Sensitivity analysis and subanalysis were also performed.</div></div><div><h3>Results</h3><div>In this meta-analysis, nine articles comprising 1,127 patients who underwent carotid stenting (606 with DLS) were studied. No significant difference between DLS and SLS was found in stroke (at 30 days DLS: 4/555 vs. SLS: 11/496; OR 0.38, 95% confidence interval [CI] 0.14–1.03; at 12 months DLS: 1/249 vs. SLS: 4/152; OR 0.21, 95% CI 0.03–1.36), death (at 30 days DLS: 3/526 vs. SLS: 0/467; OR 0.80, 95% CI 0.20–3.11; at 12 months DLS: 5/249 vs. SLS: 3/152; OR 1.12, 95% CI 0.25–5.03), stroke/death (at 30 days DLS: 6/526 vs. SLS: 11/467; OR 0.43, 95% CI 0.16–1.17; at 12 months DLS: 6/249 vs. SLS: 7/152; OR 0.52, 95% CI 0.17–1.61), new lesions (DLS: 56/202 vs. SLS: 96/254; OR 0.62, 95% CI 0.28–1.40), lesion count (MD = −0.24, 95% CI -0.82–0.34), lesion diameter (MD = −0.03, 95% CI -1.21–1.15), in-stent restenosis (ISR; DLS: 5/204 vs. SLS: 8/190; OR 0.61, 95% CI 0.21–0.74) and acute thrombosis (DLS: 4/146 vs. SLS: 1/122; OR 2.03, 95% CI 0.31–13.26). Subgroup analysis indicated that CGuard and Casper had shown similar efficacy in preventing stroke, death, and new brain lesions.</div></div><div><h3>Conclusion</h3><div>DLS has shown comparable prognoses to the first-generation stent concerning stroke, stroke/death, new magnetic resonance imaging lesion incidence, lesion count, lesion diameter, ISR and acute thrombosis. Further randomized trials are warranted to demonstrate whether patients with high-risk carotid plaques can benefit significantly from DLS.</div></div>","PeriodicalId":8061,"journal":{"name":"Annals of vascular surgery","volume":"115 ","pages":"Pages 248-260"},"PeriodicalIF":1.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Positive Association of Homocysteine (Hcy) with Arteriovenous Fistula Thrombosis (AVFT) in Chinese Patients on Hemodialysis: A Retrospective Cohort Study","authors":"Jingwei Zhang ,&nbsp;Wei Yang ,&nbsp;Yuwei Kang ,&nbsp;Shijie Ma ,&nbsp;Xuyang Luo ,&nbsp;Yi Fan ,&nbsp;Jiaojiao Du ,&nbsp;Huan Luo ,&nbsp;Xudong Wang ,&nbsp;Fei Deng ,&nbsp;Xiaoxia Geng","doi":"10.1016/j.avsg.2025.02.024","DOIUrl":"10.1016/j.avsg.2025.02.024","url":null,"abstract":"<div><h3>Background</h3><div>Homocysteine (Hcy) is generally elevated in dialysis patients and is one of the independent risk factors for thrombosis. However, the relationship between Hcy level and the risk of arteriovenous fistula thrombosis (AVFT) has not yet fully been understood. Current evidence regarding this association is limited, particularly among Chinese populations. Given the significance of this relationship in health care and public health, further investigation is essential. This study aims to clarify the association between Hcy and AVFT. In addition, it seeks to examine the synergistic effects of Hcy alongside other factors influencing AVFT. It also intends to determine how Hcy interacts with variables such as diabetes and impacts the risk of AVFT.</div></div><div><h3>Methods</h3><div>A retrospective cohort study was conducted involving 983 hemodialysis patients. Data on demographic characteristics, comorbidities, laboratory test indicators, and clinical symptoms were collected from all participants. Statistical analyses, including logistic regression, smooth curve fitting, and joint interaction effects analysis, were employed to address the research questions.</div></div><div><h3>Results</h3><div>The overall prevalence of AVFT in the study population was 16.20%. A significant association between Hcy levels and the risk of AVFT was found (odds ratio [OR], 1.14; 95% confidence interval [CI], 1.11–1.16; <em>P</em> &lt; 0.001). This association remained significant after adjusting for multiple variables (OR, 1.13; 95% CI, 1.11–1.17; <em>P</em> &lt; 0.001). Participants were categorized into high and low Hcy groups based on the median (25.2 μmol/L), with a significantly elevated risk of AVFT was observed in the high Hcy group compared to the low Hcy group (OR, 5.55; 95% CI, 3.59–8.55; <em>P</em> &lt; 0.001). This risk remained significantly elevated after adjustment for covariates (OR, 3.59; 95% CI, 1.83–7.55, <em>P</em> &lt; 0.001). Subsequent exploratory subgroup analyses revealed similar significant interactions (all <em>P</em> values for interaction &gt;0.05). Furthermore, sensitivity analyses indicated that diabetes and Hcy levels exhibit a synergistic multiplicative and additive interaction in the risk of AVFT (both <em>P</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>The findings suggest that elevated Hcy levels are linked to an increased risk of AVFT. The data highlight diabetes as a synergistic interaction factor through which Hcy influences AVFT risk. These results underscore the necessity for further research, especially considering potential confounding factors. Future studies should aim to confirm these findings and explore the underlying mechanisms of the observed associations.</div></div>","PeriodicalId":8061,"journal":{"name":"Annals of vascular surgery","volume":"115 ","pages":"Pages 261-274"},"PeriodicalIF":1.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Right-sided Aortic Torsion in Patients with Abdominal Aortic Aneurysms.","authors":"Anirudh Chandrashekar, Luis Leon, Lachlan Smith, Nicos Labropoulos","doi":"10.1016/j.avsg.2025.02.028","DOIUrl":"https://doi.org/10.1016/j.avsg.2025.02.028","url":null,"abstract":"<p><strong>Objectives: </strong>Altered flow dynamics within abdominal aortic aneurysms (AAA) may lead to changes in aneurysmal geometry, intraluminal thrombus (ILT) deposition, or aneurysmal progression. Aortic torsion is one geometric deviation that has been clinically observed but has not been formally evaluated in pre-operative AAAs. This pilot retrospective cohort study investigates the degree and directionality of aortic torsion in patients with and without AAAs.</p><p><strong>Methods: </strong>The inferior mesenteric artery (IMA-Angle) outlet angle was used to assess aortic torsion. Angles were measured with respect to the anterior-posterior axis in both aneurysmal (370) and non-aneurysmal (120) patients. Patient age, gender, maximum infrarenal aortic/AAA diameter (D_Max), and presence/percentage of ILT were calculated.</p><p><strong>Results: </strong>370 AAA patients (Age: 74 [65:83], %Male: 88%, D_Max: 50.1 mm [41.9 - 57.0 mm]) were retrospectively identified. ILT was present in 65% of cases and comprised 26.2% of the aneurysmal sac [18.6 - 36.7%]. Similarly, 120 patients without aneurysmal disease were identified (Age: 70 [63:81], %Male: 79%, D_Max: 23.3 mm [21.5 - 25.6]). Median IMA-Angle [25th-75th%] in the aneurysmal cohort was 17.0° [8.6° - 25.3°] and closer to the AP axis compared to controls (38.5° [34.3° - 44.9°], p < 0.001). Presence, percentage, and classification of ILT (r = 0.01, p = 0.93) had negligible impact on IMA outlet angle.</p><p><strong>Conclusion: </strong>This study highlights the right-sided IMA preference in AAA patients compared to non-aneurysmal controls. The pathophysiology underlying this rotation may be associated with a right-sided helical flow pattern in expanding aneurysmal sacs. This sets the foundation for future investigations.</p>","PeriodicalId":8061,"journal":{"name":"Annals of vascular surgery","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"African Research Output Within the Top 5 Vascular Surgery Journals.","authors":"Oluwatobi Shekoni, Tioluwanimi Ojeola, Oduwale Mubaraq, Adebayo Falola","doi":"10.1016/j.avsg.2025.02.017","DOIUrl":"https://doi.org/10.1016/j.avsg.2025.02.017","url":null,"abstract":"<p><strong>Background: </strong>With Africa's population growth and an epidemiological shift from communicable to non-communicable diseases, there is an urgent need to expand vascular surgery capacity across the continent. Despite a high disease burden, Sub-Saharan Africa contributes less than 1% of the global surgical research, with significant challenges in surgical infrastructure, access to consumables, and limited research output. This study assesses the publication output of African authors in the top five vascular surgery journals and explores trends in African vascular research.</p><p><strong>Methods: </strong>The top five vascular surgery journals, based on CiteScore-impact factor, were selected: Journal of Vascular Surgery (JVS), European Journal of Vascular and Endovascular Surgery (EJVES), Journal of Vascular Surgery: Venous and Lymphatic Disorders (JVS-VL), Annals of Vascular Surgery (AVS), and Journal of Endovascular Therapy (JEVT). A PubMed search identified articles authored by researchers affiliated with African institutions. After removing duplicates and exclusions, 252 articles were analyzed using SPSS v27.0.1.</p><p><strong>Results: </strong>AVS and JVS were the most frequent publication venues, accounting for 32.5% and 28.6% of articles, respectively. Egypt and South Africa were the leading contributors, producing 44.8% and 28.2% of the research, respectively. Most studies were observational (81.3%), with only 7.1% being interventional. Research output has steadily increased, with 38.1% of articles published in the 2010s and 36.5% in the 2020s.</p><p><strong>Conclusion: </strong>Vascular surgery is an emerging specialty in Africa, facing challenges, particularly in research. However, the number of publications in leading vascular surgery journals by African researchers is increasing, with Egypt and South Africa leading the way. More efforts are needed to enhance access to vascular surgery and research in Africa to boost the continent's contribution to global vascular surgery literature.</p>","PeriodicalId":8061,"journal":{"name":"Annals of vascular surgery","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interferon Regulatory Factor 1 Promotes Diabetic Vasculopathy by Mediating Endothelial Cell Pyroptosis","authors":"Yande Zhou ,&nbsp;Zhihua Liu ,&nbsp;Yangyufan Xu ,&nbsp;Jiadong Chen ,&nbsp;Jianhui Li ,&nbsp;Mingchen Zhang ,&nbsp;Mingqi Weng ,&nbsp;Xueqin Cao ,&nbsp;Ying Xie","doi":"10.1016/j.avsg.2025.02.018","DOIUrl":"10.1016/j.avsg.2025.02.018","url":null,"abstract":"<div><h3>Background</h3><div>Diabetic vasculopathy is not only one of the most common and serious complications of diabetes mellitus but also the leading cause of death in patients with type 2 diabetes mellitus (T2DM). Endothelial cells, located at the interface between blood and interstitial tissues, are the first line of defense of the vascular system against inflammatory damage, and their death is usually linked to atherosclerosis progression. However, the exact mechanisms need to be explored in greater depth.</div></div><div><h3>Methods</h3><div>GSE169332 data, including one group of no diabetic atherosclerosis and one group of diabetic atherosclerosis, were integrated and analyzed using single-cell RNA sequencing technology. The biological pathways involved and the key factor interferon regulatory factor 1 (IRF-1) were explored by Gene Ontology pathway enrichment and trajectory analysis. The role of IRF-1-mediated endothelial cell pyroptosis in diabetic model mice BKS^−db/db was verified by in vivo assays of immunofluorescence, immunohistochemistry, hematoxylin and eosin staining, and Masson staining.</div></div><div><h3>Results</h3><div>Single-cell RNA sequencing results showed that the development of diabetic vasculopathy was associated with endothelial cell pyroptosis, and endothelial pyroptosis 1 (EP1) cells with high expression of pyroptosis-associated factors exhibited a significant increase in diabetic atherosclerosis samples. The Gene Ontology enrichment results further emphasized the importance of endothelial cells in the progression of diabetic vasculopathy, especially EP1 cells. Trajectory analysis revealed that IRF-1 was more likely to be the hub gene of EP1 relative to other pyroptosis-related genes. Immunofluorescence, immunohistochemistry, hematoxylin and eosin staining, and Masson staining of mouse vascular tissues demonstrated that IRF-1 expression was significantly increased in diabetic model mice BKS^−db/db, and the expression of pyroptosis-related marker the NACHT, LRR, and PYD domains-containing protein 3 (NLRP3), as well as <strong>interleukin</strong>-<strong>1beta</strong> (IL-1β) and <strong>interleukin</strong>-<strong>1</strong>8 <strong>(</strong>IL-18) levels, was also significantly increased.</div></div><div><h3>Conclusions</h3><div>IRF-1 promotes the progression of diabetic vasculopathy by mediating endothelial cell pyroptosis through increasing the expression of pyroptosis-related marker NLRP3 and the levels of IL-1β and IL-18.</div></div>","PeriodicalId":8061,"journal":{"name":"Annals of vascular surgery","volume":"115 ","pages":"Pages 107-116"},"PeriodicalIF":1.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}