Biomolecular engineering最新文献_第2页

Symposium J: Surface functionalization and activation of biomaterials 专题研讨会J:生物材料的表面功能化和活化

Biomolecular engineering Pub Date : 2007-11-01 DOI: 10.1016/j.bioeng.2007.07.001

Hartmut F. Hildebrand, Barbara J. Nebe, Julio San Roman, Joelle Amedée

引用次数: 4

The dynamics of cholesterol molecules near the surface of protein farnesyltransferase - Computer simulation 蛋白质法尼基转移酶表面附近胆固醇分子的动力学-计算机模拟

Biomolecular engineering Pub Date : 2007-11-01 DOI: 10.1016/j.bioeng.2007.08.009

Przemysław Raczyński, Zygmunt Gburski

引用次数: 1

Fibrils of different collagen types containing immobilised proteoglycans (PGs) as coatings: Characterisation and influence on osteoblast behaviour 含有固定蛋白聚糖(pg)作为涂层的不同胶原蛋白类型的原纤维:表征及其对成骨细胞行为的影响

Biomolecular engineering Pub Date : 2007-11-01 DOI: 10.1016/j.bioeng.2007.07.008

T. Douglas , U. Hempel , C. Mietrach , S. Heinemann , D. Scharnweber , H. Worch

{"title":"Fibrils of different collagen types containing immobilised proteoglycans (PGs) as coatings: Characterisation and influence on osteoblast behaviour","authors":"T. Douglas , U. Hempel , C. Mietrach , S. Heinemann , D. Scharnweber , H. Worch","doi":"10.1016/j.bioeng.2007.07.008","DOIUrl":"10.1016/j.bioeng.2007.07.008","url":null,"abstract":"<div><p>Collagen, the main organic component of bone, is used as a coating on titanium implants and as a scaffold material in bone tissue engineering. Surface modifications of titanium which promote osteoblast adhesion, proliferation and synthesis of collagen by osteoblasts are desirable. One biomimetic approach is the coating of titanium with collagen in fibrillar form. Other organic components of bone may be bound to fibrils and exert additional effects. In this study, the collagen types I–III were compared regarding their ability to bind the proteoglycans decorin and biglycan, which are found in bone. More collagen was bound to collagen II fibrils than to those of types I and III. Therefore, titanium surfaces were coated with fibrils of collagen type II containing biglycan or decorin or neither to investigate the effect of the proteoglycans on human primary osteoblast behaviour. In addition, the growth factor TGF-β1 was adsorbed onto surfaces coated with fibrils of collagen type II containing biglycan or decorin or neither to investigate the influence of decorin and biglycan on the effect of TGF-β1 on osteoblasts. Fibril-bound biglycan and decorin influence primary osteoblast behaviour by themselves. The presence of substrate-bound biglycan or decorin influences the effect of TGF-β1. These results may be important when designing collagen-based coatings or scaffolds for tissue engineering, including those loaded with growth factors.</p></div>","PeriodicalId":80259,"journal":{"name":"Biomolecular engineering","volume":"24 5","pages":"Pages 455-458"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bioeng.2007.07.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27101553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Apatite-forming ability of alumina and zirconia ceramics in a supersaturated Ca/P solution 氧化铝和氧化锆陶瓷在过饱和Ca/P溶液中形成磷灰石的能力

Biomolecular engineering Pub Date : 2007-11-01 DOI: 10.1016/j.bioeng.2007.07.009

Sabina Beranič Klopčič, Janez Kovač, Tomaž Kosmač

引用次数: 16

Emulsion-based synthesis of PLGA-microspheres for the in vitro expansion of porcine chondrocytes 乳化剂合成用于猪软骨细胞体外扩增的plga微球

Biomolecular engineering Pub Date : 2007-11-01 DOI: 10.1016/j.bioeng.2007.08.013

Franziska Gabler , Simone Frauenschuh , Jochen Ringe , Christoph Brochhausen , Peter Götz , C. James Kirkpatrick , Michael Sittinger , Helmut Schubert , Rolf Zehbe

{"title":"Emulsion-based synthesis of PLGA-microspheres for the in vitro expansion of porcine chondrocytes","authors":"Franziska Gabler , Simone Frauenschuh , Jochen Ringe , Christoph Brochhausen , Peter Götz , C. James Kirkpatrick , Michael Sittinger , Helmut Schubert , Rolf Zehbe","doi":"10.1016/j.bioeng.2007.08.013","DOIUrl":"10.1016/j.bioeng.2007.08.013","url":null,"abstract":"<div><p>The <em>in vitro</em> cell expansion of autologous chondrocytes is of high interest in regenerative medicine since these cells can be used to treat joint cartilage defects. In order to preserve chondrocyte phenotype, while optimizing adhesion on microspheres, several processing parameters for the microsphere synthesis were varied. In this study three different polylactide-<em>co</em>-glycolides were used with differing lactide–glycolide ratios (85:15 and 50:50) and differing inherent viscosities. An emulsion route was established, where the polymer was dissolved in chloroform and then injected into a stirred polyvinyl alcohol–water solution at different polymer concentrations and different stirring velocities to produce microspheres with varying diameters. The sphere size distribution and morphology was analyzed using image processing software on SEM pictures. Based on previous experiments with commercial microspheres, three optimum samples were selected for further investigations. The degradation of the microspheres was determined in a long-term experiment in culture medium for 3 months. Adherent cells were characterized after 3 and 5 days by FDA+EB vital staining and in SEM.</p></div>","PeriodicalId":80259,"journal":{"name":"Biomolecular engineering","volume":"24 5","pages":"Pages 515-520"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bioeng.2007.08.013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41041957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Tribo-electrochemical technique for studying tribocorrosion behavior of biomaterials 研究生物材料摩擦腐蚀行为的摩擦电化学技术

Biomolecular engineering Pub Date : 2007-11-01 DOI: 10.1016/j.bioeng.2007.07.015

M. Azzi, J.A. Szpunar

{"title":"Tribo-electrochemical technique for studying tribocorrosion behavior of biomaterials","authors":"M. Azzi, J.A. Szpunar","doi":"10.1016/j.bioeng.2007.07.015","DOIUrl":"10.1016/j.bioeng.2007.07.015","url":null,"abstract":"<div><p>Tribocorrosion is the term which describes the synergy between tribological and electrochemical processes. An apparatus was designed and built to study the tribocorrosion behavior of biomaterials. Electrochemical set-up with three electrodes is used for controlling the potential of the surface of a conducting material subjected to classical wear testing. Using this equipment, it is possible to carry out friction and wear tests in electrolytic solution under well-defined electrochemical conditions determined by the applied electrode potential. In this paper, this apparatus was described and the tests of deposited TiN on pure Ti for corrosion and tribocorrosion behavior under simulated body fluid were conducted. The presence of TiN layer on the surface of Ti has increased the open circuit potential. The charge transfer resistance (<em>R</em><sub>ct</sub>) determined using electrochemical impedance spectroscopy (EIS) was higher for the nitrided surfaces than for the Ti substrates. However after wear test, <em>R</em><sub>ct</sub> was significantly reduced because the protective layer was damaged.</p></div>","PeriodicalId":80259,"journal":{"name":"Biomolecular engineering","volume":"24 5","pages":"Pages 443-446"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bioeng.2007.07.015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41041960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 80

Carbon encapsulated magnetic nanoparticles for biomedical applications: Thermal stability studies 用于生物医学应用的碳封装磁性纳米颗粒:热稳定性研究

Biomolecular engineering Pub Date : 2007-11-01 DOI: 10.1016/j.bioeng.2007.08.006

Michał Bystrzejewski , Stanisław Cudziło , Andrzej Huczko , Hubert Lange , Gervais Soucy , German Cota-Sanchez , Waldemar Kaszuwara

引用次数: 50

Silica sol–gel matrix doped with Photolon molecules for sensing and medical therapy purposes 掺杂光子分子的硅溶胶-凝胶基质用于传感和医学治疗

Biomolecular engineering Pub Date : 2007-11-01 DOI: 10.1016/j.bioeng.2007.07.005

Halina Podbielska , Agnieszka Ulatowska-Jarza , Gerhard Müller , Iwona Holowacz , Joanna Bauer , Uwe Bindig

{"title":"Silica sol–gel matrix doped with Photolon molecules for sensing and medical therapy purposes","authors":"Halina Podbielska , Agnieszka Ulatowska-Jarza , Gerhard Müller , Iwona Holowacz , Joanna Bauer , Uwe Bindig","doi":"10.1016/j.bioeng.2007.07.005","DOIUrl":"10.1016/j.bioeng.2007.07.005","url":null,"abstract":"<div><p>Photolon is one of the new photosensitisers that has found application in photodynamic therapy (PDT). Its chemical structure has a partially reduced porphyrin moiety and its molecular structure is comparable to chlorin e<sub>6</sub>, which can be isolated after hydrolysis of the 5-membered exocyclic β-ketoester moiety of pheophorbide a.</p><p>For this study, a Photolon doped sol–gel matrix was produced in the form of coatings deposited on silica fibers cores. The material was produced from sols prepared from the silicate precursor TEOS mixed with ethyl alcohol. The sol–gel films were prepared with factor <em>R</em>    <!-->rpm). The coated fibers were examined in different environments, liquid and gaseous, at different pH values and with various zinc cation concentrations. The chemical reactions were studied by means of spectroscopic methods, whereby the fluorescence response was studied. It was demonstrated that Photolon immobilized in a sol–gel matrix is accessible for the environment and shows visible response to the external changes. Furthermore, it was observed that these reactions are reversible. These biomaterials are also examined as carriers for PDT. It was also proved that a toxic effect is observed an environment with microorganisms, meaning that doped coatings have photodynamic activity.</p></div>","PeriodicalId":80259,"journal":{"name":"Biomolecular engineering","volume":"24 5","pages":"Pages 425-433"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bioeng.2007.07.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27006923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Dextran and hyaluronan methacrylate based hydrogels as matrices for soft tissue reconstruction 右旋糖酐和甲基丙烯酸透明质酸水凝胶作为软组织重建基质

Biomolecular engineering Pub Date : 2007-11-01 DOI: 10.1016/j.bioeng.2007.08.014

Stephanie Möller , Jürgen Weisser , Sabine Bischoff , Matthias Schnabelrauch

{"title":"Dextran and hyaluronan methacrylate based hydrogels as matrices for soft tissue reconstruction","authors":"Stephanie Möller , Jürgen Weisser , Sabine Bischoff , Matthias Schnabelrauch","doi":"10.1016/j.bioeng.2007.08.014","DOIUrl":"10.1016/j.bioeng.2007.08.014","url":null,"abstract":"<div><p>Polysaccharide hydrogels have become increasingly studied as matrices in soft tissue engineering because of their known cytocompatibility. In this work cross-linkable dextran methacrylates and hyaluronan methacrylate were synthesized and their transformation into stable hydrogels was studied. The in vitro degradation behaviour of the formed hydrogels could be controlled by the polysaccharide structure and the cross-linking density. Under in vitro conditions, the formed gels had no cytotoxic effects against fibroblasts, but cells could adhere only inefficiently in long term experiments. The use of composite gels improved the adherence of cells. Different scaffold architectures were studied including porous structures and perforated gel layers.</p><p>Selected hydrogels were examined in an in vivo pilot study using a rabbit model to evaluate their biocompatibility, stability and degradation. No signs of inflammation were seen and with prolonged duration the material was degraded and lacunas were formed by immigrating or ingrowing cells. Optimizing their mechanical properties, the formed hydrogels represent promising candidates as matrices for soft tissue reconstruction.</p></div>","PeriodicalId":80259,"journal":{"name":"Biomolecular engineering","volume":"24 5","pages":"Pages 496-504"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bioeng.2007.08.014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40986431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 73

Biological and physico-chemical assessment of hydroxyapatite (HA) with different porosity 不同孔隙度羟基磷灰石(HA)的生物学和理化评价

Biomolecular engineering Pub Date : 2007-11-01 DOI: 10.1016/j.bioeng.2007.08.015

J.-C. Hornez , F. Chai , F. Monchau , N. Blanchemain , M. Descamps , H.F. Hildebrand

{"title":"Biological and physico-chemical assessment of hydroxyapatite (HA) with different porosity","authors":"J.-C. Hornez , F. Chai , F. Monchau , N. Blanchemain , M. Descamps , H.F. Hildebrand","doi":"10.1016/j.bioeng.2007.08.015","DOIUrl":"10.1016/j.bioeng.2007.08.015","url":null,"abstract":"<div><p>HA with specific internal porosities was loaded with different antibiotics (ATBs) and then tested on its microbiological effectiveness. The HA purity was controlled with X-ray diffraction, IR and Raman spectrometry. Varying the sintering temperature and/or adding graphite and PMMA as porogenous agents lead to obtained micro- and meso-porosities. The biological tests concerned cell viability, proliferation and morphology (SEM), and the cytochemical staining of actin and vinculin. The micro- and meso-porous HA samples had an internal pore size of 1–10μm and 10–50μm, respectively. X-ray diffraction and FTIR confirmed the high purity of the HA. The <em>cell viability tests</em> with L132 cells confirmed the excellent cytocompatibility of HA, the graphite powder and the ATB vancomycine. <em>Proliferation rate</em> was assessed with MC3T3-E1 osteoblasts. All HA samples produced a higher proliferation than the controls; the micro-porous HA inducing the highest cell growth. The ATB impregnated HA also stimulated cell proliferation but in lower extend. Cytochemical staining of osteoblasts revealed a well-developed <em>cytoskeleton</em> with strong stress fibres. Labelling of the focal adhesion contacts with anti-vinculin showed a less developed adhesion process in the cells on the different HA substrates. It was possible to realize a highly pure hydroxyapatite with different but controlled porosities by varying the sintering temperature and/or addition of a porogenous agents. This purity and the micro-porosity stimulate significantly cell growth.</p></div>","PeriodicalId":80259,"journal":{"name":"Biomolecular engineering","volume":"24 5","pages":"Pages 505-509"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bioeng.2007.08.015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40999668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 70