Ukrains'kyi biokhimichnyi zhurnal (1999 )最新文献

[The effect of Ca(2+)-induced opening of cyclosporine-sensitive pore on the oxygen consumption and functional state of rat liver mitochondria]. [Ca(2+)诱导的环孢素敏感孔开放对大鼠肝脏线粒体耗氧量和功能状态的影响]。

Ukrains'kyi biokhimichnyi zhurnal (1999 ) Pub Date : 2013-09-01 DOI: 10.15407/ubj85.05.037

O V Akopova, V I Nosar', I N Man'kovskaia, V F Sagach

{"title":"[The effect of Ca(2+)-induced opening of cyclosporine-sensitive pore on the oxygen consumption and functional state of rat liver mitochondria].","authors":"O V Akopova, V I Nosar', I N Man'kovskaia, V F Sagach","doi":"10.15407/ubj85.05.037","DOIUrl":"https://doi.org/10.15407/ubj85.05.037","url":null,"abstract":"The effect of Ca(2+)-induced opening of cyclosporine-sensitive pore (mitochondrial permeability transition pore, MPTP) on the oxygen consumption and mitochondrial functional state was studied in the rat liver mitochondria. It was shown that, with the use of glutamate as oxidation substrate, in the absence of depolarization MPTP opening results in the increase of steady state respiration rate because of the activation of cyclosporine-sensitive Ca2+/H(+)-exchange and Ca2+ cycling, which was supported by the simultaneous work of MPTP and Ca(2+)-uniporter. With the aid of selective blockers, cyclosporine A and ruthenium red, it was shown that MPTP and Ca(2+)-uniporter contribute equally to the Ca(2+)-cycling and mitochondrial respiration. It was shown that bioenergetic effects of MPTP opening under steady state conditions (increase in the oxygen consumption rate under substrate oxidation without ADP, decrease in respiratory control ratio as well as the effectiveness of ATP synthesis, P/O) are close to the functional alterations, which result from the increase of endogenous proton conductance of mitochondrial membrane. Uncoupling effect of MPTP opening, by itself, had no effect on phosphorylation rate, which remains relatively stable because the fall of P/O is compensated by the activation of respiratory chain and the increase in the rate of state 3 respiration. It was concluded that under physiologically normal conditions MPTP might function as the endogenous mechanism of mild uncoupling of respiratory chain.","PeriodicalId":80245,"journal":{"name":"Ukrains'kyi biokhimichnyi zhurnal (1999 )","volume":"85 5","pages":"37-49"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32076563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

[The effect of N-stearoylethanolamine on the activity of antioxidant enzymes, content of lipid peroxidation products and nitric oxide in the blood plasma and liver of rats with induced insulin-resistance]. [n-硬脂酰乙醇胺对胰岛素抵抗大鼠血浆和肝脏中抗氧化酶活性、脂质过氧化产物和一氧化氮含量的影响]。

Ukrains'kyi biokhimichnyi zhurnal (1999 ) Pub Date : 2013-09-01

O V Onopchenko, H V Kosiakova, T M Horid'ko, A H Berdyshev, O F Mehed', N M Hula

{"title":"[The effect of N-stearoylethanolamine on the activity of antioxidant enzymes, content of lipid peroxidation products and nitric oxide in the blood plasma and liver of rats with induced insulin-resistance].","authors":"O V Onopchenko, H V Kosiakova, T M Horid'ko, A H Berdyshev, O F Mehed', N M Hula","doi":"","DOIUrl":"","url":null,"abstract":"The influence of N-stearoylethanolamine (NSE) on the content of lipid peroxidation products, activity of antioxidant enzymes and the nitric oxide level in the liver and blood plasma of rats with insulin-resistance (IR) state was investigated. IR state was induced in rats by prolonged high-fat diet (58% of energy derived from fat) for 6 months combined with one injection of streptozotocin (15 mg/kg of body weight). The existence of IR state was estimated by results of glucoso-tolerance test and blood plasma insulin content. The level of lipid peroxides products was shown to be higher in the liver of insulin resistant animals as a result of reduced superoxide dismutase and catalase activity, however, glutathione peroxidase activity was increased. The increase of nitric-oxide content in the liver and blood plasma of high-fat diet rats compared with healthy control animals was also observed. The administration of the NSE suspension per os in a dose of 50 mg/kg during 2 weeks to the rats with induced insulin-resistance state contributed to the increase of superoxide dismutase, catalase and glutathione peroxidase activity. In consequence of antioxidant enzymes activation the intensity of POL process was decreased. The NSE administration caused normalization of nitric oxide level, restoring pro-/antioxidant balance in the liver and blood plasma of rats with IR state. In conclusion, the NSE administration to the rats with insulin-resistance state restored pro-/antioxidant balance and enhanced the content of nitric oxide, therefore, improving insulin sensitivity.","PeriodicalId":80245,"journal":{"name":"Ukrains'kyi biokhimichnyi zhurnal (1999 )","volume":"85 5","pages":"88-96"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32076568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Carbohydrate restriction in the larval diet causes oxidative stress in adult insects of Drosophila melanogaster]. [限制幼虫饮食中的碳水化合物会导致黑腹果蝇成虫的氧化应激]。

Ukrains'kyi biokhimichnyi zhurnal (1999 ) Pub Date : 2013-09-01 DOI: 10.15407/ubj85.05.061

B M Rovenko, V I Lushchak, O V Lushchak

{"title":"[Carbohydrate restriction in the larval diet causes oxidative stress in adult insects of Drosophila melanogaster].","authors":"B M Rovenko, V I Lushchak, O V Lushchak","doi":"10.15407/ubj85.05.061","DOIUrl":"https://doi.org/10.15407/ubj85.05.061","url":null,"abstract":"The influence of 20 and 1% glucose and fructose, which were components of larval diet, on the level of oxidized proteins and lipids, low molecular mass antioxidant content as well as activities of antioxidant and associated enzymes in adult fruit fly Drosophila melanogaster were investigated. The restriction of carbohydrates in larval diet leads to oxidative stress in adult insects. It is supported by 40-50% increased content of protein carbonyl groups and by 60-70% decreased level of protein thiol groups as well as by a 4-fold increase of lipid peroxide content in 2-day-old flies of both sexes, developed on the diet with 1% carbohydrates. Oxidative stress, induced by carbohydrate restriction of the larval diet, caused the activation of antioxidant defence, differently exhibited in male and female fruit flies. Caloric restriction increased activity of superoxide dismutase and thioredoxin reductase associating only in males with 2-fold higher activity of NADPH-producing enzymes--glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase. Carbohydrate restriction in the larval diet caused the increase of uric acid content, but the decrease in catalase activity in males. In females the values of these parameters were changed in opposite direction compared with males. The obtained results let us conclude the different involvement of low molecular mass antioxidants, glutathione and uric acid, and antioxidant enzyme catalase in the protection of male and female fruit fly macromolecules against oxidative damages, caused by calorie restriction of larval diet.","PeriodicalId":80245,"journal":{"name":"Ukrains'kyi biokhimichnyi zhurnal (1999 )","volume":"85 5","pages":"61-72"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32076565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

[Isolation and characterization of new monoclonal antibodies against human IgE]. [新型人IgE单克隆抗体的分离与鉴定]。

Ukrains'kyi biokhimichnyi zhurnal (1999 ) Pub Date : 2013-09-01

O Iu Halkin, A A Savchenko, K I Nikitina, O M Duhan

引用次数: 0

[Identification of components of the milky juice of Lactarius pergamenus (Fr.)Fr fungi by gas-liquid chromatography/mass spectrometry]. [气液色谱/质谱法鉴定pergamenus (Fr.)Fr真菌乳汁成分]。

Ukrains'kyi biokhimichnyi zhurnal (1999 ) Pub Date : 2013-09-01

M V Tsyvins'ka, L V Panchak, R S Stoĭka, V O Antoniuk

{"title":"[Identification of components of the milky juice of Lactarius pergamenus (Fr.)Fr fungi by gas-liquid chromatography/mass spectrometry].","authors":"M V Tsyvins'ka, L V Panchak, R S Stoĭka, V O Antoniuk","doi":"","DOIUrl":"","url":null,"abstract":"The authors have proposed method of separation of methanol extract of Lactarius pergamenus basidiomes and investigation of fractions extracted with hexane which, according to our previous studies, possess the highest antiproliferative and antifungal activity. Main attention was given to fractionation and analysis by gas-liquid chromatography-mass spectrometry (GLC/MS) of the fraction 1.4 extracted with hexane. The key role in separation of this fraction was played by the use of dioxane which mixes well with both polar and nonpolar solvents (hexane, chloroform, methanol, water). Chemical composition of hexanoic fraction of methanol extract of dried basidomes Lactarius pergamenus fungi were completely characterized. It was found that this fraction consisted of 38% of fatty acids and their derivatives, 29%--of the phthalates, 13%--of the sesquiterpene, 2%--of aldehydes and 18%--of other compounds (hydrocarbons, alcohols, hydrazine derivatives and unidentified substances). Such combination of constituents allows forming a stable emulsion of milky juice which protects the mushroom fruit body from the bacterial and fungal infections and from eating by the mammalians and insects.","PeriodicalId":80245,"journal":{"name":"Ukrains'kyi biokhimichnyi zhurnal (1999 )","volume":"85 5","pages":"170-6"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32076399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-organization and fractality in a metabolic processes of the Krebs cycle. 克雷布斯循环代谢过程中的自组织和分形。

Ukrains'kyi biokhimichnyi zhurnal (1999 ) Pub Date : 2013-09-01 DOI: 10.15407/ubj85.05.191

V I Grytsay, I V Musatenko

{"title":"Self-organization and fractality in a metabolic processes of the Krebs cycle.","authors":"V I Grytsay, I V Musatenko","doi":"10.15407/ubj85.05.191","DOIUrl":"https://doi.org/10.15407/ubj85.05.191","url":null,"abstract":"The metabolic processes of the Krebs cycle is studied with the help of a mathematical model. The autocatalytic processes resulting in both the formation of the self-organization in the Krebs cycle and the appearance of a cyclicity of its dynamics are determined. Some structural-functional connections creating the synchronism of an autoperiodic functioning at the transport in the respiratory chain and the oxidative phosphorylation are investigated. The conditions for breaking the synchronization of processes, increasing the multiplicity of cyclicity, and for the appearance of chaotic modes are analyzed. The phase-parametric diagram of a cascade of bifurcations showing the transition to a chaotic mode by the Feigenbaum scenario is obtained. The fractal nature of the revealed cascade of bifurcations is demonstrated. The strange attractors formed as a result of the folding are obtained. The results obtained give the idea of structural-functional connections, due to which the self-organization appears in the metabolism running in a cell. The constructed mathematical model can be applied to the study of the toxic and allergic effects of drugs and various substances on cell metabolism.","PeriodicalId":80245,"journal":{"name":"Ukrains'kyi biokhimichnyi zhurnal (1999 )","volume":"85 5","pages":"191-200"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32075364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

[Substrate specificity of Cryptococcus albidus and Eupenicillium erubescens alpha-L-rhamnosidases]. [性隐球菌和红绿拟青霉α - l -鼠李糖苷酶的底物特异性]。

Ukrains'kyi biokhimichnyi zhurnal (1999 ) Pub Date : 2013-09-01 DOI: 10.15407/ubj85.05.017

O V Hudzenko, L D Varbanets'

{"title":"[Substrate specificity of Cryptococcus albidus and Eupenicillium erubescens alpha-L-rhamnosidases].","authors":"O V Hudzenko, L D Varbanets'","doi":"10.15407/ubj85.05.017","DOIUrl":"https://doi.org/10.15407/ubj85.05.017","url":null,"abstract":"The substrate specificity of Cryptococcus albidus and Eupenicillium erubescens alpha-L-rhamnosidases has been investigated. It is shown that the enzymes are able to act on synthetic and natural substrates, such as naringin, neohesperidin. alpha-L-Rhamnosidases hydrolysed the latter ones very efficiently, in this case E. erubescens enzyme was characterized by higher values of V(max) in comparison with the enzyme of C. albidus. However the C. albidus alpha-L-rhamnosidase showed greater affinity for naringin and neohesperidin than the enzyme of E. erubescens (K(m) 0.77 and 3.3 mM and 5.0 and 3.0 mM, respectively). As regards the synthetic derivatives of monosaccharides, both enzymes exhibited narrow specificity for glycon: E. erubescens alpha-L-rhamnosidase--only to the p-nitrophenyl-alpha-L-rhamnopiranoside (K(m) 1.0 mM, V(max) 120 micromol/min/mg protein), and C. albidus--to p-nitrophenyl-alpha-D-glucopyranoside (K(m) 10 mM, V(max) 5 micromol/min/mg protein). Thus, it was found that the enzyme preparations of E. erubescens and C. albidus are differed by their substrate specificity. The ability of E. erubescens and C. albidus alpha-L-rhamnosidases to hydrolyse natural substrates: naringin and neohesperidin, evidences for their specificity for alpha-1,2-linked L-rhamnose. Based on these data, we can predict the use of E. erubescens and C. albidus alpha-L-rhamnosidases in various industries, food industry in particular. This is also confirmed by the fact that the investigated alpha-L-rhamnosidases were stable at 20% concentration of ethanol and 500 mM glucose in the reaction mixture.","PeriodicalId":80245,"journal":{"name":"Ukrains'kyi biokhimichnyi zhurnal (1999 )","volume":"85 5","pages":"17-26"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32075526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Oxidative stress in blood leukocytes, pro/antioxidant status and fatty acids composition of pancreas lipids at experimental acute pancreatitis in rats]. [实验性急性胰腺炎大鼠血白细胞氧化应激、前/抗氧化状态和胰腺脂质脂肪酸组成]。

Ukrains'kyi biokhimichnyi zhurnal (1999 ) Pub Date : 2013-09-01

I B Pryvrots'ka, T M Kuchmerovs'ka

{"title":"[Oxidative stress in blood leukocytes, pro/antioxidant status and fatty acids composition of pancreas lipids at experimental acute pancreatitis in rats].","authors":"I B Pryvrots'ka, T M Kuchmerovs'ka","doi":"","DOIUrl":"","url":null,"abstract":"In an experimental model of acute pancreatitis (AP) in rats no alteration in leukocyte's viability was found by flow cytometry as compared to control. After 1 day of AP production of reactive oxygen forms in granulocytes was increased more than 5 times, but after 3 days their level was decreased. Alterations of pro/antioxidant status and specific changes in the fatty acid composition in the pancreas were established. With the development of AP, the processes of lipids peroxidation were intensified while antioxidant system was altered, that was evidenced by inflammation in the pancreas. In these conditions, the increase of phospholipase A2 activity was accompanied by significant changes of fatty acid composition of the total lipids in the pancreas. This increased relative total content of saturated fatty acids, in particular myristic, palmitic and stearic acid increased, while the total content of polyunsaturated essential fatty acids omega-3 (linolenic, eicosapentaenoic, dokozapentayenoic, docosahexaenoic) decreased. The preparation containing omega-3 polyunsaturated fatty acids partially normalized the lipid and fatty acids composition as well as prooxidant-antioxidant system.","PeriodicalId":80245,"journal":{"name":"Ukrains'kyi biokhimichnyi zhurnal (1999 )","volume":"85 5","pages":"124-36"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32074308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Arginase and NO-synthase pathways of L-arginine metabolism in peripheral blood lymphocytes of patients with ovarian cancer]. [卵巢癌患者外周血淋巴细胞l -精氨酸代谢的精氨酸酶和no合成酶途径]。

Ukrains'kyi biokhimichnyi zhurnal (1999 ) Pub Date : 2013-09-01 DOI: 10.15407/ubj85.05.105

O I Iakubets', R V Fafula, D Z Vorobets', Z D Vorobets'

引用次数: 3

[Kinetic pattern of folic acid release from polyurethane matrices of varied structures]. [不同结构聚氨酯基质中叶酸释放的动力学模式]。

Ukrains'kyi biokhimichnyi zhurnal (1999 ) Pub Date : 2013-09-01

M V Hryhor'ieva

引用次数: 0