Journal of clinical laser medicine & surgery最新文献

{"title":"Interstitial laser hyperthermia and the biological characteristics of tumor: study in a murine model of colorectal liver metastases.","authors":"V Muralidharan,&nbsp;M Nikfarjam,&nbsp;C Malcontenti-Wilson,&nbsp;Christopher Christophi","doi":"10.1089/104454703765035493","DOIUrl":"https://doi.org/10.1089/104454703765035493","url":null,"abstract":"<p><strong>Background data: </strong>Percutaneously applied interstitial laser hyperthermia (ILH) is a minimally invasive therapy that is currently used in the treatment of liver metastases. Despite its documented efficacy, theoretical considerations and evidence based on animal studies suggest the potential for stimulating tumor growth, especially following surgery. This study investigates the influence of ILH on tumor behaviour in an animal model of colorectal liver metastases.</p><p><strong>Materials and methods: </strong>A model of colorectal cancer liver metastases in male inbred CBA mice was used. Laser hyperthermia was applied to tumor tissue using a bare optical quartz from a Medilas fibertom 4100 Nd:YAG surgical laser generator. Liver injury by ILH was initially produced in three experimental groups of animals at different time points in the development of metastases. ILH was applied (i) to normal liver 10 days prior to tumor induction, (ii) immediately prior to tumor induction, and (iii) 15 days after tumor induction to achieve approximately 8% liver destruction. Animals were killed 21 days after tumor induction, and the effects of ILH on overall tumor development were compared with controls using stereological assessment of tumor volume and by histology. In a separate experimental group, the effects of ILH on fully established tumors were examined. Suitable tumors were selected 21 days after induction and partially destroyed by ILH at a standard energy setting. Animals were then killed 15 days later, and the growth rate of the residual viable tumors was compared to control tumors having undergone sham procedures.</p><p><strong>Results: </strong>No significant stimulation of tumor growth was evident in any of the experimental groups following ILH, irrespective of the time of application. Incomplete tumor destruction also had no influence on subsequent tumor growth.</p><p><strong>Conclusion: </strong>ILH does not influence the biological characteristics of tumors during any stage of the metastatic process.</p>","PeriodicalId":79503,"journal":{"name":"Journal of clinical laser medicine & surgery","volume":"21 2","pages":"75-83"},"PeriodicalIF":0.0,"publicationDate":"2003-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/104454703765035493","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22377616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"He-Ne laser on microcrystalline arthropathies.","authors":"V Campana,&nbsp;M Moya,&nbsp;A Gavotto,&nbsp;J C Simes,&nbsp;L Spitale,&nbsp;F Soriano,&nbsp;J A Palma","doi":"10.1089/104454703765035529","DOIUrl":"https://doi.org/10.1089/104454703765035529","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this work is to assess the anti-inflammatory capacity of He-Ne laser therapy as determined by the plasmatic levels of inflammatory markers, fibrinogen, and TNFalpha and by histopathological study in rats with arthropathy induced by calcium pyrophosphate crystals.</p><p><strong>Background data: </strong>Microcrystalline arthropathies are a group of diseases characterized by the deposit of different crystals in joints.</p><p><strong>Materials and methods: </strong>Two milligrams of dicalcium pyrophosphate crystals (DCPP) were injected in both joints of the lower limbs of rats during 2 days. A group was treated with laser of He-Ne (6 mW) on the injected joints during 3 consecutive days. After 96 h of the first injection, animals were sacrificed to determine TNFalpha using the ELISA method and fibrinogen was assessed using spectrophotometry. Sections from the lower limbs were used for histopathology.</p><p><strong>Results: </strong>A statistically significant increase (p < 0.001) in plasma fibrinogen levels and TNFalpha was noted between the control group and the laser-treated group. The histological transversal section of a posterior limb joint of a rat injected with DCPP showed fibroadipose tissue with diffuse chronic infiltrate. The histopathology of the group of rats injected with DCPP and subsequently treated with He-Ne laser showed no inflammatory response.</p><p><strong>Conclusion: </strong>He-Ne laser treatment in the microcrystalline arthropathy induced in rats by DCPP injection might have an antiinflammatory effect, evaluated by fibrinogen plasma levels and TNF-alpha (inflammatory markers) and by the histopathology regressive process.</p>","PeriodicalId":79503,"journal":{"name":"Journal of clinical laser medicine & surgery","volume":"21 2","pages":"99-103"},"PeriodicalIF":0.0,"publicationDate":"2003-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/104454703765035529","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22377619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser literature watch.","authors":"","doi":"10.1089/10445470360516761","DOIUrl":"https://doi.org/10.1089/10445470360516761","url":null,"abstract":"","PeriodicalId":79503,"journal":{"name":"Journal of clinical laser medicine & surgery","volume":"21 1","pages":"51-5"},"PeriodicalIF":0.0,"publicationDate":"2003-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/10445470360516761","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22270216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological alterations after irradiation of cartilage using Ho:YAG laser.","authors":"O Sahap Atik,&nbsp;Deniz Erdogan,&nbsp;Suna Omeroglu,&nbsp;Feza Korkusuz,&nbsp;M Murad Uslu,&nbsp;Fatih Eksioglu","doi":"10.1089/10445470360516671","DOIUrl":"https://doi.org/10.1089/10445470360516671","url":null,"abstract":"<p><strong>Objective: </strong>The authors carried out an experimental study to evaluate the histological alterations after irradiation of cartilage using Holmium:YAG (Ho:YAG) laser VersaPulse Select 60 watts and Infra Tome Delivery Systems 30 degrees Handpiece (spot size at fiber tip 0.4 mm; Coherent Medical, Palo Alto, CA).</p><p><strong>Background data: </strong>Recently, some authors reported cases with articular cartilage damage following arthroscopic laser surgery in the knee joint for the treatment of articular cartilage or meniscal pathology.</p><p><strong>Materials and methods: </strong>The cartilage specimens immersed in saline were exposed to Ho:YAG laser irradiation. The laser wavelength was 2.1 microm, and pulse duration was 250 microsec. Power settings were 0.2-1 joules per pulse and 10-15 Hz. Total laser energy used in these procedures was 1.5 K joules. During the procedures, the handpiece was used at an angle of 30 degrees and at a distance of 1 mm.</p><p><strong>Results: </strong>Electron microscopic evaluation demonstrated that the ultrastructure of the cartilage is preserved in both experiment and control groups.</p><p><strong>Conclusion: </strong>When Ho:YAG laser is used in optimal dosage (optimal joule and Hertz) with optimal technique (keeping the handpiece at an appropriate angle and distance) and avoiding overtreatment, it does not cause cartilage damage.</p>","PeriodicalId":79503,"journal":{"name":"Journal of clinical laser medicine & surgery","volume":"21 1","pages":"3-6"},"PeriodicalIF":0.0,"publicationDate":"2003-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/10445470360516671","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22270289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of the Er:YAG laser irradiation on titanium implant materials and contaminated implant abutment surfaces.","authors":"Tomoko Matsuyama,&nbsp;Akira Aoki,&nbsp;Shigeru Oda,&nbsp;Takayuki Yoneyama,&nbsp;Isao Ishikawa","doi":"10.1089/10445470360516680","DOIUrl":"https://doi.org/10.1089/10445470360516680","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of this study was to examine the morphological changes and temperature increases of the titanium after Er:YAG laser irradiation, and also to investigate the effect of this laser on debridement of contaminated healing abutments.</p><p><strong>Background data: </strong>Mechanical instruments have been used for the cleaning of implant abutment surfaces, however, most of them are not appropriate for the application to titanium surface. Recently, the Er:YAG laser has been expected to have a promising ability for the debridement of implant surface.</p><p><strong>Materials and methods: </strong>Experiments were composed of three parts. At first, ten titanium round plates were exposed to the Er:YAG laser irradiation at 30-200 mJ/pulse and the surface changes were observed by stereomicroscope and scanning electron microscope. Secondly, the surface temperature changes of 60 titanium plates during and after Er:YAG laser irradiation at 30 and 50 mJ/pulse were measured by thermographic equipment. At last, calculus on the surface of six contaminated healing abutments was removed by Er:YAG laser or ultrasonic scaler, and the treated surfaces were examined by stereomicroscope.</p><p><strong>Results: </strong>Under 50 mJ/pulse, distinct morphological changes were not observed and the elevation of surface temperature was minimal, especially in the use of water-cooling. The Er:YAG laser at 30 mJ/pulse and 30 Hz with water spray was capable of effectively removing plaque and calculus on the implant abutments without injuring their surfaces.</p><p><strong>Conclusion: </strong>This study indicates that the Er:YAG laser can be a novel technical modality for the debridement of implant abutment surface.</p>","PeriodicalId":79503,"journal":{"name":"Journal of clinical laser medicine & surgery","volume":"21 1","pages":"7-17"},"PeriodicalIF":0.0,"publicationDate":"2003-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/10445470360516680","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22270290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer effect of combined intratumor cisplatin injection and interstitial KTP laser therapy on xenografted squamous cell carcinoma.","authors":"Phil-Sang Chung,&nbsp;Han-Gyun Kim,&nbsp;Chung-Ku Rhee,&nbsp;Romaine E Saxton","doi":"10.1089/10445470360516707","DOIUrl":"https://doi.org/10.1089/10445470360516707","url":null,"abstract":"<p><strong>Objective: </strong>This study was conducted to evaluate the synergistic effect of cisplatin and interstitial KTP laser therapy induced hyperthermia in vitro and in vivo (interstitial laser chemotherapy, ILCT).</p><p><strong>Methods: </strong>In vitro study: human hypopharyngeal squamous carcinoma cell line (SNU-1041) was incubated in 1 mL of media containing various concentrations (0, 1, 2, 5, 10, 20, 50, 100 ng/mL) of cisplatin in 37 degrees and 43 degrees C for 2 hours. The viability of the cell was evaluated by MTT assay. In vivo study: human squamous cell carcinoma tumors were grown as subcutaneous transplants in nude mice and injected into tumor with cisplatin-epigel 100 microg, followed by interstitial laser therapy (ILT) via 0.6-mm diffuser fiber tips (532 nm, 1.0J/mm(3)).</p><p><strong>Results: </strong>In vitro study: the viability of tumor cells incubated with 10 ng/mL of cisplatin was 62% and 28% in 37 degrees C and 43 degrees C, respectively (p = 0.02). In vivo study: the tumor volume in 4 weeks after treatment was 179 mm(3) in ILT group and 27 mm(3) in ILCT group. The ILCT group showed 8 (80%) complete remission of tumors at 4 weeks follow up, while tumor remission occurred in only 3 (30%) in ILT group (p < 0.05).</p><p><strong>Conclusion: </strong>These results suggest ILCT may become an effective treatment for head and neck carcinoma with minimal functional deficit.</p>","PeriodicalId":79503,"journal":{"name":"Journal of clinical laser medicine & surgery","volume":"21 1","pages":"23-7"},"PeriodicalIF":0.0,"publicationDate":"2003-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/10445470360516707","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22270212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Nd:YAG laser irradiation on the hardness and elastic modulus of human dentin.","authors":"Bor Shiunn Lee,&nbsp;Chun Pin Lin,&nbsp;Feng Huei Lin,&nbsp;Uei Ming Li,&nbsp;Wan Hong Lan","doi":"10.1089/10445470360516734","DOIUrl":"https://doi.org/10.1089/10445470360516734","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of this study was to investigate the changes of hardness and elastic modulus of human dentin after Nd:YAG laser irradiation.</p><p><strong>Background data: </strong>The application of Nd:YAG laser in dental hard tissues has been widely studied. However, little information is available about the mechanical properties of teeth after Nd:YAG laser irradiation.</p><p><strong>Methods: </strong>The human dentin was irradiated by Nd:YAG laser through a 400-microm optic bare fiber. The parameters in laser delivery were 100 mJ/pulse--10 pps--4 sec and 150 mJ/pulse--10 pps--4 sec. Both the hardness and elastic modulus were obtained using an Instron microhardness tester and Vickers indenter. The indentations were then examined under a scanning electron microscope.</p><p><strong>Results: </strong>The hardness and elastic modulus of irradiated human dentin in the energy of 100 mJ/pulse--10 pps--4 sec and 150 mJ/pulse--10 pps--4 sec were 44.7 kg/mm(2) and 22.8 GPa, and 46.9 kg/mm(2) and 21.4 GPa, respectively. These values were significantly lower than that of non-irradiated dentin by the Student's t test.</p><p><strong>Conclusion: </strong>Our study demonstrated that Nd:YAG laser irradiation would reduce the hardness and elastic modulus of human dentin.</p>","PeriodicalId":79503,"journal":{"name":"Journal of clinical laser medicine & surgery","volume":"21 1","pages":"41-6"},"PeriodicalIF":0.0,"publicationDate":"2003-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/10445470360516734","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22270215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethics, education, common sense and medicine.","authors":"Raymond J Lanzafame","doi":"10.1089/10445470360516662","DOIUrl":"https://doi.org/10.1089/10445470360516662","url":null,"abstract":"","PeriodicalId":79503,"journal":{"name":"Journal of clinical laser medicine & surgery","volume":"21 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2003-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/10445470360516662","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22270288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of linearly polarized near-infrared irradiation on deformability of human stored erythrocytes.","authors":"Kozo Yokoyama,&nbsp;Kazuna Sugiyama","doi":"10.1089/10445470360516699","DOIUrl":"https://doi.org/10.1089/10445470360516699","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the influence of linearly polarized near-infrared irradiation using the Super Lizer trade mark on deformability of human erythrocytes.</p><p><strong>Background data: </strong>Not only low-powered laser but also linearly polarized near-infrared beams have some biostimulation effects on various tissues. There were some reports of erythrocyte deformability improved by low-powered He-Ne laser irradiation.</p><p><strong>Materials and methods: </strong>Human erythrocyte samples stored for three weeks were adjusted to 30% hematocrit. Erythrocyte deformability presented as the filter filtration rate was measured.</p><p><strong>Results: </strong>There was no difference of the filter filtration rate between control group without irradiation and the group of 125 mJ/cm(2) exposure level at a wavelength of 830 nm. However, the groups of 625 and 1,250 mJ/cm(2) exposure levels at a wavelength of 830 nm showed higher filter filtration rates compared to the control group.</p><p><strong>Conclusion: </strong>Linearly polarized near-infrared irradiation in a range of 625-1,250 mJ/cm(2) exposure level at a wavelength of 830 nm improved deformability of human stored erythrocytes.</p>","PeriodicalId":79503,"journal":{"name":"Journal of clinical laser medicine & surgery","volume":"21 1","pages":"19-22"},"PeriodicalIF":0.0,"publicationDate":"2003-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/10445470360516699","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22270291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study on compositional changes and Knoop hardness measurement of the cavity floor prepared by Er:YAG laser irradiation and mechanical bur cavity.","authors":"Mozammal Hossain,&nbsp;Yukio Nakamura,&nbsp;Yoshiko Murakami,&nbsp;Yoshishige Yamada,&nbsp;Koukichi Matsumoto","doi":"10.1089/10445470360516716","DOIUrl":"https://doi.org/10.1089/10445470360516716","url":null,"abstract":"<p><strong>Objective: </strong>This present study was performed to compare the compositional changes of human dentin and, Knoop hardness of the cavity floor prepared by Er:YAG laser irradiation with that of the conventional bur cavity.</p><p><strong>Background data: </strong>There are still no reports on the compositional changes of dental hard tissues and microhardness of the cavity floor prepared by Er:YAG laser irradiation.</p><p><strong>Materials and methods: </strong>Fifteen laser and 15 bur cavities were cross-sectioned, and subjected to atomic analysis by SEM-EDX and Knoop hardness testing. Statistical analyses were performed using the Mann-Whitney U test; a value of p < 0.01 was considered significant. Cross sections of the remaining five laser and five bur cavities were examined by light microscopy and then by SEM.</p><p><strong>Results: </strong>The results showed that the quantities of Ca (Ca weight %) and P (P weight %) were increased significantly in the laser cavities, but no significant differences were found between the Ca/P ratio and Knoop hardness number of laser and bur cavities. The results of SEM observation revealed that the lased cavity surface was irregular, and there was also the absence of a smear layer; the orifice of dentinal tubules was exposed.</p><p><strong>Conclusion: </strong>Er:YAG laser device produces minimal thermal induced changes of dental hard tissue compositions; Ca/P ratio and Knoop hardness of the lased cavity floor was almost similar to the bur cavities.</p>","PeriodicalId":79503,"journal":{"name":"Journal of clinical laser medicine & surgery","volume":"21 1","pages":"29-33"},"PeriodicalIF":0.0,"publicationDate":"2003-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/10445470360516716","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22270213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}