Journal of lipid mediators and cell signalling最新文献_第10页

Production and function of lipid second messengers in proliferating and differentiated neuroblastoma cells 神经母细胞瘤细胞增殖分化过程中脂质第二信使的产生和功能

Journal of lipid mediators and cell signalling Pub Date : 1996-09-01 DOI: 10.1016/0929-7855(96)00544-5

D. Lang , J.N. Kanfer , G. Goracci , L. Freysz

{"title":"Production and function of lipid second messengers in proliferating and differentiated neuroblastoma cells","authors":"D. Lang , J.N. Kanfer , G. Goracci , L. Freysz","doi":"10.1016/0929-7855(96)00544-5","DOIUrl":"10.1016/0929-7855(96)00544-5","url":null,"abstract":"<div>Multiple cellular responses are regulated through the generation of lipid second messengers upon activation of phospholipases. One such response concerns the activity of a class of kinase constituting the protein kinase C family. The production of specific molecular species of lipid second messengers may be therefore of prime importance in the activation of a member of the PKC isoforms. Prompted by this possibility we investigated the production of 1,2 diacyl-sn-glycerol (DAG) and phosphatidic acid (PtdOH) in LA-N-1 neuroblastoma cells under various physiological states. 12-0-Tetradecanoylphorbol 13-acetate (TPA) stimulation activated a phospholipase D (PLD) specific for phosphatidylcholine (PtdCho) in proliferating cells and a phospholipase C (PLC) specific for phosphatidylethanolamine (PtdEtn) in retinoic acid (RA) differentiated cells. These separate activations produced different molecular species of DAG or PtdOH. PtdOH was able to stimulate the Ca2+ dependent protein kinase C (PKC) by a mechanism which differed from the action of DAG. PtdOH did not induce the translocation of the PKC to the membrane. Moreover PtdOH, in contrast to DAG, prevented PKC degradation by inhibiting the enzymatic hydrolysis by m-calpain. These observations suggest that the stimulation of cells by agonists elicited the production of specific molecular species of lipid second messengers depending on the physiological status of the cells, and probably on the nature of the stimulus. It seems therefore likely that the generation of specific lipid second messengers may activate specific PKC isoforms resulting in a specific cellular response.</div>","PeriodicalId":79347,"journal":{"name":"Journal of lipid mediators and cell signalling","volume":"14 1","pages":"Pages 349-359"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0929-7855(96)00544-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19872008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Author index to vol. 14 第14卷的作者索引

Journal of lipid mediators and cell signalling Pub Date : 1996-09-01 DOI: 10.1016/S0929-7855(96)90003-6

引用次数: 0

Ceramide: Role in growth inhibitory cascades 神经酰胺:生长抑制级联反应中的作用

Journal of lipid mediators and cell signalling Pub Date : 1996-09-01 DOI: 10.1016/0929-7855(96)00538-X

Supriya Jayadev, Yusuf A. Hannun

引用次数: 19

Lysosphingomyelin-elicited Ca2+ mobilization from rat brain microsomes 溶鞘磷脂诱导大鼠脑微粒体Ca2+动员

Journal of lipid mediators and cell signalling Pub Date : 1996-09-01 DOI: 10.1016/0929-7855(96)00539-1

Shigeki Furuya , Sadamu Kurono , Yoshio Hirabayashi

引用次数: 12

Membrane-associated phospholipase D activity in neural cell line PC12 膜相关磷脂酶D在神经细胞系PC12中的活性

Journal of lipid mediators and cell signalling Pub Date : 1996-09-01 DOI: 10.1016/0929-7855(96)00531-7

Yoshiko Banno , Yuzuru Ito , Katsuhiro Ojio , Hiroyuki Kanoh , Shigeru Nakashima , Yoshinori Nozawa

引用次数: 7

Membrane localization of N-acylphosphatidylethanolamine in central neurons: Studies with exogenous phospholipases n -酰基磷脂酰乙醇胺在中枢神经元中的膜定位:外源性磷脂酶的研究

Journal of lipid mediators and cell signalling Pub Date : 1996-09-01 DOI: 10.1016/0929-7855(96)00510-X

Hugues Cadas, Sergio Schinelli , Daniele Piomelli

{"title":"Membrane localization of N-acylphosphatidylethanolamine in central neurons: Studies with exogenous phospholipases","authors":"Hugues Cadas, Sergio Schinelli , Daniele Piomelli","doi":"10.1016/0929-7855(96)00510-X","DOIUrl":"10.1016/0929-7855(96)00510-X","url":null,"abstract":"<div>We studied the localization of N-acyl phosphatidylethanolamine (NAPE), a putative cannabinoid precursor, in primary cultures of striatal and cortical neurons from the rat brain. We probed intact neurons with various exogenous phospholipases, including S. chromofuscus phospholipase D (PLD). S. chromofuscus PLD does not penetrate into neurons (as demonstrated by a lack of internalization of 125I-labeled PLD), and does not cause gross damage to the neuronal membrane (as demonstrated by a lack of effect of PLD on [3H]γ-aminobutyric acid release). When neurons, labeled to isotopic equilibrium with [3H]ethanolamine, were incubated for 10 min with S. chromofuscus PLD, approximately 50% of neuronal NAPE was hydrolysed. This hydrolysis was accompanied by the release of a family of N-acyl ethanolamines (NAE) (assessed by high performance liquid chromatography), which included the cannabinoid receptor agonist, anandamide. Exogenous phospholipase A2 (PLA2) (Apis mellifera) and PLC (B. cereus) mobilized [3H]arachidonate and [3H]diacylglycerol, respectively, but had no effect on NAE formation under these conditions. These experiments indicate that ∼ 50% of neuronal NAPE is localized in a compartment that is easily accessible to extracellular PLD, possibly the plasmalemma, where it would also be easily hydrolyzed upon stimulation to produce NAE.</div>","PeriodicalId":79347,"journal":{"name":"Journal of lipid mediators and cell signalling","volume":"14 1","pages":"Pages 63-70"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0929-7855(96)00510-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19871614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Retrograde messengers and long-term potentiation: A progress report 逆行信使和长期增强:进展报告

Journal of lipid mediators and cell signalling Pub Date : 1996-09-01 DOI: 10.1016/0929-7855(96)00542-1

J.H. Williams

引用次数: 23

Heterogeneity of diacylglycerol kinase in terms of molecular structure, biochemical characteristics and gene expression localization in the brain 二酰基甘油激酶在脑内分子结构、生化特性和基因表达定位方面的异质性

Journal of lipid mediators and cell signalling Pub Date : 1996-09-01 DOI: 10.1016/0929-7855(96)00533-0

Kaoru Goto, Hisatake Kondo

{"title":"Heterogeneity of diacylglycerol kinase in terms of molecular structure, biochemical characteristics and gene expression localization in the brain","authors":"Kaoru Goto, Hisatake Kondo","doi":"10.1016/0929-7855(96)00533-0","DOIUrl":"10.1016/0929-7855(96)00533-0","url":null,"abstract":"<div>Three different cDNA clones for diacylglycerol (DG) kinase were isolated from a rat brain cDNA library and designated DGK-I, DGK-II and DGK-III. These three encode distinct polypeptides with 58% identity to each other and contain EF-hand motifs, cysteine-rich zinc finger-like sequences and putative ATP-binding sites. A high kinase activity is shown in COS cells transfected with either one of the three cDNAs without substrate specificity among DG species, and the kinase activity is Ca-dependent. The activity for DGK-I is recovered dominantly in the soluble fraction of the cell, that for DGK-II in the particulate fraction; and that for DGK-III equally in both of the fractions. The difference in their expression localization is most noticeable: DGK-I is expressed in oligodendrocytes of the brain as well as T-lymphocytes in the thymus and spleen; DGK-II is expressed in neurons of the caudate-putamem, accumbens nucleus and olfactory tubercle; and DGK-III in the cerebellar Purkinje cells and granule cells. The functional significance of the discovery of three DG kinase isozymes is briefly discussed.</div>","PeriodicalId":79347,"journal":{"name":"Journal of lipid mediators and cell signalling","volume":"14 1","pages":"Pages 251-257"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0929-7855(96)00533-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19870758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Phosphatidylcholine hydrolysis and protein kinase C activation for intracellular signaling network 磷脂酰胆碱水解和蛋白激酶C在细胞内信号网络中的激活

Journal of lipid mediators and cell signalling Pub Date : 1996-09-01 DOI: 10.1016/0929-7855(96)00525-1

Shun-ichi Nakamura

引用次数: 14

Lipid messengers in the nervous system. Proceedings of a satellite symposium of the 15th meeting of the International Society for Neurochemistry. Tokyo, Japan, June 28-30, 1995. 神经系统中的脂质信使。第十五届国际神经化学学会卫星研讨会论文集。1995年6月28日至30日，日本东京。

Journal of lipid mediators and cell signalling Pub Date : 1996-09-01

引用次数: 0