Cell adhesion and communication最新文献_第3页

Adhesion-induced intracellular signalling in endothelial cells depends on the nature of the matrix. 内皮细胞粘附诱导的细胞内信号传导取决于基质的性质。

Cell adhesion and communication Pub Date : 1999-01-01 DOI: 10.3109/15419069909034390

N Berge, L D Loganadane, J Vassy, E Monnet, C Legrand, F Fauvel-Lafeve

{"title":"Adhesion-induced intracellular signalling in endothelial cells depends on the nature of the matrix.","authors":"N Berge, L D Loganadane, J Vassy, E Monnet, C Legrand, F Fauvel-Lafeve","doi":"10.3109/15419069909034390","DOIUrl":"https://doi.org/10.3109/15419069909034390","url":null,"abstract":"The adhesion of a human microvascular endothelial cell line to its own matrix was studied in comparison with adhesion of the same cells to fibronectin or thrombospondin-1. These endothelial cells adhered preferentially to their matrix whereas an equal cell number was attached to fibronectin or thrombospondin-1. The adhesion of cells to thrombospondin-1 was mediated by the N-terminal heparin binding domain of thrombospondin-1 as shown by the use of a recombinant fragment, N18. Cells adhering to their matrix displayed a morphology and a cytoskeleton organization very similar to that observed in vivo with an apical immunostaining for actin stress fibers and a fine basal labeling for vinculin. Cells on fibronectin were extensively spread and rapidly assembled stress fibers and focal contacts. Cells adherent to thrombospondin-1 presented large lamellae rich in actin but devoid of vinculin and only few actin fibers were observed. Depending on the substratum used, adhering endothelial cells displayed also different tyrosine phosphorylation patterns on electrophoresis. Our observations indicate that endothelial cells adhering to their matrix present an activation state intermediate between that induced by a \"hyperadhesive\" protein like fibronectin and that generated by a moderate, indeed anti-adhesive, protein like thrombospondin-1.","PeriodicalId":79325,"journal":{"name":"Cell adhesion and communication","volume":"7 1","pages":"29-41"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/15419069909034390","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21099280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Characterization of the signaling pathways regulating alpha2beta1 integrin-mediated events by a pharmacological approach. 通过药理学方法表征调节alpha2beta1整合素介导事件的信号通路。

Cell adhesion and communication Pub Date : 1999-01-01 DOI: 10.3109/15419069909010799

V Petit, B Boyer, J P Thiery, A M Vallés

{"title":"Characterization of the signaling pathways regulating alpha2beta1 integrin-mediated events by a pharmacological approach.","authors":"V Petit, B Boyer, J P Thiery, A M Vallés","doi":"10.3109/15419069909010799","DOIUrl":"https://doi.org/10.3109/15419069909010799","url":null,"abstract":"In certain instances of developing and adult organism, epithelial cells can change morphology and transform into mesenchymal-like type in order to move through the extracellular matrix. However, because of the multiplicity and complexity of signaling pathways that contribute to these processes, their molecular dissection has remained difficult. By using a pharmacological approach on the rat bladder carcinoma cell line NBT-II dispersion system, we have identified distinct signaling events for adhesion and motility in response to collagen, both activities depending on alpha2beta1 integrin. Treatment of cells with PKC inhibitors markedly impaired initial attachment on collagen without affecting the capacity of cells to move, suggesting that PKC activity is required for initial adhesion strength during cell translocation. Both adhesion and motility were diminished by tyrosine kinase inhibitors herbimycin and tyrphostin whereas tyrosine phosphatase inhibitors amplified cell scattering. The collagen-induced dispersion was insensitive to genistein which we previously showed to abrogate growth factor-induced scattering, thus demonstrating inducer specificity. Finally. Ras inhibitors and expression of a dominant negative form of Ras (N17Ras) while affecting initial cell attachment, did not prevent cell migration, and instead favored the dissociated state on collagen. The specific signaling pathways identified for adhesion and motility should help to understand the sequential processes associated with cell migration.","PeriodicalId":79325,"journal":{"name":"Cell adhesion and communication","volume":"7 3","pages":"151-65"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/15419069909010799","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21482168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Pertussis toxin treatment in vivo reduces surface expression of the adhesion integrin leukocyte function antigen-1 (LFA-1). 体内百日咳毒素处理可降低粘附整合素白细胞功能抗原-1 (LFA-1)的表面表达。

Cell adhesion and communication Pub Date : 1999-01-01 DOI: 10.3109/15419069909010801

A R Schenkel, C D Pauza

{"title":"Pertussis toxin treatment in vivo reduces surface expression of the adhesion integrin leukocyte function antigen-1 (LFA-1).","authors":"A R Schenkel, C D Pauza","doi":"10.3109/15419069909010801","DOIUrl":"https://doi.org/10.3109/15419069909010801","url":null,"abstract":"Pertussis toxin treatment in macaques inhibits lymphocyte extravasation from the blood and leads to transient lymphocytosis and leukocytosis. We examined lymphocyte adhesion molecules known to be involved in the extravasation process to find possible mechanisms for the effects of pertussis toxin treatment. The two subunits of LFA-1, CD11a and CD18, showed decreased surface expression on lymphocytes from pertussis toxin treated animals compared to untreated animals. The adhesion molecule CD44, and the alpha subunit of the integrin VLA-4 (CD49d) were not decreased by pertussis toxin treatment. Lower surface expression of CD11a and CD18 was observed on all lymphocyte subsets and was correlated inversely with the extent of lymphocytosis. The magnitude of lymphocytosis after pertussis toxin treatment was higher in SIV-infected macaques than in uninfected animals. However, changes in LFA-1 levels were similar in both groups. These data show that LFA-1 surface levels are affected by pertussis toxin in vivo and this change may account in part, for the ability of pertussis toxin to induce lymphocytosis.","PeriodicalId":79325,"journal":{"name":"Cell adhesion and communication","volume":"7 3","pages":"183-93"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/15419069909010801","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21482170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Integrin-dependent tyrosine phosphorylation and growth regulation by Vav. 整合素依赖性酪氨酸磷酸化及Vav对生长的调节作用。

Cell adhesion and communication Pub Date : 1999-01-01 DOI: 10.3109/15419069909034388

I Yron, M Deckert, M E Reff, A Munshi, M A Schwartz, A Altman

{"title":"Integrin-dependent tyrosine phosphorylation and growth regulation by Vav.","authors":"I Yron, M Deckert, M E Reff, A Munshi, M A Schwartz, A Altman","doi":"10.3109/15419069909034388","DOIUrl":"https://doi.org/10.3109/15419069909034388","url":null,"abstract":"The proto-oncogene product p95Vav (Vav) undergoes rapid phosphorylation on tyrosine following stimulation of the T or B cell antigen receptor, and in response to a variety of other cell surface stimuli. Vav contains, among other, a guanine nucleotide exchange factor domain with homology to the Rho/Rac/CDC42 exchange protein Db1. It has been recently shown that Vav is functionally linked to small GTPases of the Rho family, suggesting that it is an activator of Rho GTPases and may participate in regulation of cytoskeletal organization. The present study shows that cell adhesion to fibronectin triggers rapid phosphorylation of Vav on tyrosine in Vav-transfected CHO cells and in Jurkat T cells. Vav phosphorylation is strongly dependent on adhesion and is mediated by beta 1 integrins. Furthermore, Vav overexpression enhances the adhesion-dependent increase in the rate and extent of phosphorylation on focal adhesion kinase and paxillin, and the formation of stress fibers and lamellipodia. In addition, there is a marked increase in the amount of Vav localized to the triton-insoluble fraction following 1 h of incubation on FN. Finally, Vav increases the growth rate of the cells in an adhesion-dependent manner. Our results strongly implicate Vav as a mediator of integrin signal transduction.","PeriodicalId":79325,"journal":{"name":"Cell adhesion and communication","volume":"7 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/15419069909034388","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21099278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

Targeting of cytoskeletal linker proteins to focal adhesion complexes is reduced in fibroblasts adhering to laminin-1 when compared to fibronectin. 与纤维连接蛋白相比，成纤维细胞粘附层粘连蛋白-1的细胞骨架连接蛋白靶向黏附复合物的能力降低。

Cell adhesion and communication Pub Date : 1999-01-01 DOI: 10.3109/15419069909034391

H Sondermann, D Dogic, M Pesch, M Aumailley

{"title":"Targeting of cytoskeletal linker proteins to focal adhesion complexes is reduced in fibroblasts adhering to laminin-1 when compared to fibronectin.","authors":"H Sondermann, D Dogic, M Pesch, M Aumailley","doi":"10.3109/15419069909034391","DOIUrl":"https://doi.org/10.3109/15419069909034391","url":null,"abstract":"Cellular interactions with the extracellular matrix determine to a large extent cell behavior, including cell migration. These interactions take place at specialized cellular structures, the focal adhesions, which have a substrate-specific morphology. To determine the molecular and functional relevance of this observation, the composition of isolated focal adhesions developed by fibroblasts adhering to fibronectin or laminin-1 was analyzed by indirect immunofluorescence and immunoblotting with or without stabilization of the structures by cross-linking. In the absence of cross-linking, integrins, talin, vinculin and, to a lower extent, paxillin remained associated with the focal adhesions formed on both substrates, indicating a tight association of these proteins with the extracellular matrix support. By contrast, alpha-actinin, FAK, and actin were apparently loosely maintained within focal adhesions and were found associated to these structures only after stabilization by cross-linking. Interestingly, although both substrates induced clustering and aggregation of all these proteins, their relative concentration, with the exception of alpha-actinin, was lower within the focal adhesions formed on laminin-1 than in those formed on fibronectin. Moreover, as assessed in migration assays, the locomotory speed of fibroblasts was higher on laminin-1 than on fibronectin. Altogether these results indicate that integrins involved in cellular interactions with fibronectin or laminin-1 trigger the formation of focal adhesion structures which differ by molecular organization, concentration in several adhesion plaque components, and function.","PeriodicalId":79325,"journal":{"name":"Cell adhesion and communication","volume":"7 1","pages":"43-56"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/15419069909034391","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21099281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

The N-cadherin/catenin complex in colon fibroblasts and myofibroblasts. 结肠成纤维细胞和肌成纤维细胞中的n -钙粘蛋白/连环蛋白复合物。

Cell adhesion and communication Pub Date : 1999-01-01 DOI: 10.3109/15419069909034397

L Van Hoorde, K Braet, M Mareel

引用次数: 26

The expression of adhesion molecules on endothelial cells is inhibited by troglitazone through its antioxidant activity. 曲格列酮通过其抗氧化活性抑制内皮细胞粘附分子的表达。

Cell adhesion and communication Pub Date : 1999-01-01 DOI: 10.3109/15419069909010804

L Cominacini, U Garbin, A F Pasini, A Davoli, M Campagnola, A Rigoni, L Tosetti, V Lo Cascio

{"title":"The expression of adhesion molecules on endothelial cells is inhibited by troglitazone through its antioxidant activity.","authors":"L Cominacini, U Garbin, A F Pasini, A Davoli, M Campagnola, A Rigoni, L Tosetti, V Lo Cascio","doi":"10.3109/15419069909010804","DOIUrl":"https://doi.org/10.3109/15419069909010804","url":null,"abstract":"The adhesion of monocytes to endothelium, an early event in atherosclerosis, is mediated by cell adhesion molecules. Signal-transduction pathways for these binding molecules include the translocation of the transcription factor NF-kappaB; moreover, intracellularly generated oxygen-derived free radicals (ODFR) play a major role in this process. This study evaluated the extent to which troglitazone, an oral antidiabetic agent with antioxidant properties, affects the expression of intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and E-selectin on human umbilical vein endothelial cells (HUVECs), induced by different prooxidant signals such as oxidized LDL and tumor necrosis factor-alpha (TNF-alpha). Furthermore we assessed whether the NF-kappaB activation is modulated by the antioxidative effect of troglitazone. Oxidized LDL not only caused a dose-dependent increase of ICAM-1, VCAM-1 and E-selectin (p<0.001), but also synergically increased their TNF-alpha-induced expression (p<0.001). Troglitazone reduced in a dose-dependent manner the expression of VCAM-1, ICAM-1 and E-selectin induced by different amounts of oxidized LDL (p<0.001). The addition of troglitazone to HUVECs significantly reduced the expression of ICAM-1, VCAM-1 and E-selectin induced by TNF-alpha alone or in combination with oxidized LDL (p<0.001); this reduction was paralleled by a significant fall in NF-kappaB translocation. The results suggest that troglitazone may have prevented NF-kappaB-mediated adhesion molecule expression by exerting its antioxidant effect on ODFR.","PeriodicalId":79325,"journal":{"name":"Cell adhesion and communication","volume":"7 3","pages":"223-31"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/15419069909010804","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21485255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 52

Increased expression of integrin and receptor tyrosine kinase genes during autograft fusion in the sponge Geodia cydonium. 整合素和受体酪氨酸激酶基因在海地草自体移植物融合过程中的表达增加。

Cell adhesion and communication Pub Date : 1999-01-01 DOI: 10.3109/15419069909034395

W Wimmer, B Blumbach, B Diehl-Seifert, C Koziol, R Batel, R Steffen, I M Müller, W E Müller

{"title":"Increased expression of integrin and receptor tyrosine kinase genes during autograft fusion in the sponge Geodia cydonium.","authors":"W Wimmer, B Blumbach, B Diehl-Seifert, C Koziol, R Batel, R Steffen, I M Müller, W E Müller","doi":"10.3109/15419069909034395","DOIUrl":"https://doi.org/10.3109/15419069909034395","url":null,"abstract":"Recently cDNAs coding for cell surface molecules have been isolated from sponges. The molecules for alpha-integrin, galectin, and receptor tyrosine kinase (RTK), obtained from the marine sponge, Geodia cydonium, have been described earlier. In the present study also the cDNA for one putative beta-integrin has been identified from G. cydonium. The deduced aa sequence comprises the characteristic signatures, found in other metazoan beta-integrin molecules; the estimated size is 95,215 Da. To obtain first insights into the molecular events which proceed during autograft fusion, the expressions of these genes were determined on transcriptional and translational level. The cDNAs as well as antibodies raised against the recombinant sponge proteins alpha-integrin, RTK and galectin were used and Northern blot experiments and immunocytochemical analyses have been performed. The results show that transcription of the two subunits of an integrin receptor as well as of the RTK are strongly upregulated after grafting; levels of > 10-fold have been determined in the fusion zone of the grafts after a 10 days incubation. Immunofluorescence studies of sections through the fusion zone support these data. In contrast the transcription of the gene encoding galectin is drastically downregulated after grafting. In a parallel series of experiments the level of the heat-shock protein-70 was determined and it was found that it remained unchanged after grafting. We conclude that integrin subunits and the RTK molecule are involved in self-self recognition of sponge.","PeriodicalId":79325,"journal":{"name":"Cell adhesion and communication","volume":"7 2","pages":"111-24"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/15419069909034395","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21293188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Constitutive expression of GlyCAM-1 core protein in the rat cochlea. GlyCAM-1核心蛋白在大鼠耳蜗中的组成性表达。

Cell adhesion and communication Pub Date : 1999-01-01 DOI: 10.3109/15419069909010807

N Kanoh, C F Dai, T Tanaka, D Izawa, Y F Li, H Kawashima, M Miyasaka

{"title":"Constitutive expression of GlyCAM-1 core protein in the rat cochlea.","authors":"N Kanoh, C F Dai, T Tanaka, D Izawa, Y F Li, H Kawashima, M Miyasaka","doi":"10.3109/15419069909010807","DOIUrl":"https://doi.org/10.3109/15419069909010807","url":null,"abstract":"Glycosylation-dependent cell adhesion molecule-1 (GlyCAM-1) is a mucin-like glycoprotein previously identified on high endothelial venules (HEV) of lymph nodes and also in lactating mammary glands. A specifically glycosilated form of GlyCAM-1 on HEV has been shown to be a ligand for a leukocyte L-selectin, which plays an important role in leukocyte rolling along the inflamed endothelium. Here we report that GlyCAM-1 is also expressed in the cochlea. Immunohistochemistry revealed the lateral wall of the cochlea, tectorial membrane, modiolus, organ of corti, and spiral modiolar vein (SMV) to be strongly stained with polyclonal anti-GlyCAM-1 antibody. Moreover, RT-PCR of the cochlear tissue by the use of specific oligonucleotide primers for rat GlyCAM-1 generated a 378 bp product which was then verified by nucleotide sequencing to represent GlyCAM-1. Electron microscopic investigation revealed the presence of GlyCAM-1 over the entire lumenal surface of the vessels, and the basolateral infoldings in stria vascularis. However, soluble L-selectin or mAb MECA-79 which recognizes a carbohydrate epitope on functional L-selectin ligands bound only to the spiral ligament, tectorial membrane and modiolus. These observations suggest that GlyCAM-1 expressed in the cochlear region is heterogenous in terms of its glycosylation.","PeriodicalId":79325,"journal":{"name":"Cell adhesion and communication","volume":"7 3","pages":"259-66"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/15419069909010807","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21485258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Borrelia burgdorferi downregulates ICAM-1 on human synovial cells in vitro. 伯氏疏螺旋体体外下调人滑膜细胞的ICAM-1。

Cell adhesion and communication Pub Date : 1999-01-01 DOI: 10.3109/15419069909034398

H J Girschick, S Meister, H Karch, H I Huppertz

{"title":"Borrelia burgdorferi downregulates ICAM-1 on human synovial cells in vitro.","authors":"H J Girschick, S Meister, H Karch, H I Huppertz","doi":"10.3109/15419069909034398","DOIUrl":"https://doi.org/10.3109/15419069909034398","url":null,"abstract":"Lyme arthritis following infection with Borrelia burgdorferi (B. burgdorferi) is associated with the presence of bacteria in the joint, but the mechanism of persistent infection in the presence of specific antibodies and lymphocytes remains unknown. To investigate how an infection with B. burgdorferi might influence the local immune response in the joint, we examined the expression of cell adhesion molecules, human leucocyte antigens and inducible nitric oxide synthase (iNOS)-1 and -2 in human synovial cells after infection with B. burgdorferi in vitro. Synovial cells are known to influence the function of local immunologic effector cells and play a key role in the pannus formation of erosive arthritis. It has been shown previously that B. burgdorferi can persist in the cytosol of human synovial cells. The expression of the surface molecules ICAM-1, VCAM-1, HLA-class-I and -class-II and the cytosolic production of iNOS-1 and -2 in synovial cells was measured by flow cytometry for up to 5 days after infection with B. burgdorferi. A significant, lasting downregulation of surface ICAM-1 could be demonstrated on synovial cells, whereas no significant changes were seen in the expression of VCAM-1, HLA-class-I and -II, and of iNOS-1 and -2. To determine the biological significance of this downregulation an in vitro adhesion assay using peripheral blood mononuclear cells was developed. After infection with B. burgdorferi a significantly smaller number of mononuclear cells was adhering to the synovial cell monolayer. Adhesion of peripheral mononuclear cells was shown to be in part mediated by ICAM-1 by using a blocking mononuclear antibody against ICAM-1. Downregulation of ICAM-1 on synovial cells due to infection with B. burgdorferi might suppress the local immunosurveillance and might help the bacteria to persist in joint cells in vivo.","PeriodicalId":79325,"journal":{"name":"Cell adhesion and communication","volume":"7 2","pages":"73-83"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/15419069909034398","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21293185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14