alexandria journal of veterinary sciences最新文献

{"title":"External Morphological Description of Adult Tortoise (Testudo graeca graeca) and Terrapin (Trachemys scripta elegans)","authors":"Nermin Sharnobey, Mohamed Alsafy, Samir Gendy, Mohamed Abumandour, Basma Hanafy","doi":"10.5455/ajvs.164668","DOIUrl":"https://doi.org/10.5455/ajvs.164668","url":null,"abstract":"Our study was focused on the external features differentiation of two adult species of turtles, including a land tortoise (Testudo graeca graeca) and a freshwater turtle (Trachemys scripta elegans), by gross morphological description of head, carapace, plastron, and tail. Generally, turtles show gross morphological differences according to the ecology and the environment. The carapace and legs are the fascinating noticeable parts showing significant differences between tortoise and turtle as T. graeca graeca shows a small carapace length with noticeable convexity. In contrast, the freshwater turtle has a flattened broad carapace adapted to swimming. At the same time, the legs of T. graeca show multiple keratinized spurs with rigid claws, but T. scripta has legs covered with soft, moist skin and thin claws. The plastron is relatively the same in both species, while the head is unique with a large size and red flap in T. scripta. We conclude that the flat body and legs with broad head are a modification for swimming, while the rigid claws with keratinized outer leg skin are signs of land walking adaptation.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134882982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Characterization and Antimicrobial Susceptibility of Mycoplasma Species Isolated from Broilers and Breeder Chickens","authors":"Esraa Fekry, Eman Abdeen, Youserya Hashem, Abdelaziz Abdelaziz, E E, Alaa Mostapha","doi":"10.5455/ajvs.148713","DOIUrl":"https://doi.org/10.5455/ajvs.148713","url":null,"abstract":"Mycoplasmas are considered as important avian pathogens, which cause a great economical loss in the poultry industry. As it responsible for both respiratory disease and synovitis in poultry. The aim of this study was to isolate and identify Mycoplasma gallisepticum and Mycoplasma synoviae from chickens and determine the efficacy of different antibiotics. Additionally, detection of some substantial virulence genes. Two hundred chicken samples were collected and cultured onto specific PPLO medium .The isolates were characterized by polymerase chain reaction then tested for antibiotic sensitivity by the minimum inhibitory concentration (MIC) method. The results reported that 15 samples (7.5%) were positive by culturing with prevalent of Mycoplasma synoviae and Mycoplasma gallisepticum with 12 (80%) and three (20%). Following the 16S rRNA-based detection of Mycoplasma isolates, 12 Mycoplasma synoviae and three Mycoplasma synoviae isolates were identified by different PCR-based detection methods for various virulence genes. Three Mycoplasma gallisepticum isolates contain the mgc2 gene, and nine Mycoplasma synoviae isolates were positive for the ISR (intergenic spacer region) gene. All isolates had lowest MIC values for Tylvasolin with a range of (0.062-0.125μg/mL) followed by Lincomycin, Tiamulin, Tilmicosin and Tylosin with a range of (0.062-1 μg/mL). However, the Mycoplasma synoviae isolates displayed variance in MICs for Oxytetracycline with a range of 0.5 to 8 μg/mL, and Chlorotetracycline with a range of 2 to 8 μg/mL. Meanwhile, high MIC values for Enrofloxacin were detected in all isolates with MICs ranging from 8 to 32 μg/mL. Furthermore, the MIC method identified Tylvasolin, Lincomycin, Tiamulin, Tilmicosin and Tylosin as the antibiotics of choice for the treatment of MS infections. In conclusion, these data may help in prevention and control of Mycoplasma infection in poultry.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"144 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134883369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Klebsiella pneumoniae isolated from Contaminated Sheep and Goat Meat at Matrouh Governorate and Its Antibiotic Resistance","authors":"Alaa Elhameed, Samy Khaliel, Helmy Torky","doi":"10.5455/ajvs.166298","DOIUrl":"https://doi.org/10.5455/ajvs.166298","url":null,"abstract":"The study of Klebsiella pneumoniae as a food born pathogen that isolated from sheep and goat meat at Matrouh Governorate, and tracking the extent of its resistance to various antibiotics, is considered the first study at Matrouh Governorate. Out of 48 meat samples, 6 (12.5%) were positive for Klebsiella pneumoniae subspecies pneumoniae by PCR. All Klebsiella pneumoniae isolates (100%) showed presence of fim H and aerobactin genes. All isolates showed resistance to at least 3 different classes of antibiotics, so, considered as multi drug resistant (MDR) or pan-drug resistant. The 2 pan-drug resistant strains were extended spectrum beta lactamase (ESBL) producers.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134883774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced Studies on Toxoplasma in Buffalo Meat","authors":"D. Hussein, A. Khashaba, Ayman M. Kamar","doi":"10.5455/ajvs.148101","DOIUrl":"https://doi.org/10.5455/ajvs.148101","url":null,"abstract":"Since buffalo meat, has been demonstrated to be a potential source of human infection, a careful evaluation of the prevalence of Toxoplasma infection in this meat is needed. Tissue cysts of Toxoplasma gondii are frequently found in the skeletal muscles of buffaloes. This study evaluated the prevalence of Toxoplasma gondii in the meat juice of buffalo meat samples via several diagnostic techniques to protect public health. Peptic digestion, histopathology, and serology were performed on meat juice from 100 buffalo meat samples from local butchers and retail beef markets. Eighteen samples (18%) were suspected of the presence of bradyzoites after digestion, and were subjected to histopathology which illustrated that only six samples (6%) were suspected to be Toxoplasma tissue cysts. After that periodic acid Schiff (PAS) stain confirmed that only three samples (3%) were Toxoplasma. Finally, enzyme-linked immunosorbent assay (ELISA) asserted that those three samples were Toxoplasma gondii. This study provides significant evidence about risk of human exposure to Toxoplasma through the consumption of raw or undercooked buffalo meat potentially contaminated with infectious tissue cysts.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83747004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Haemato-Biochemical Evaluation of Undifferentiated Calf Diarrhea with Special Reference to Acute Phase Response","authors":"Zeinab K. Ebrahim, Ibrahim Abdullaziz","doi":"10.5455/ajvs.153947","DOIUrl":"https://doi.org/10.5455/ajvs.153947","url":null,"abstract":"<jats:p />","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83968088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protectant Effect of Betaine Against Florfenicol-Induced Hepatorenal Toxicity in Broiler Chickens","authors":"A. Elkomy, S. Belih, F. Elsayed, Elrefaey Aboelftouh, Hawary Ibrahiem","doi":"10.5455/ajvs.146911","DOIUrl":"https://doi.org/10.5455/ajvs.146911","url":null,"abstract":"Abstract The present study was designed to evaluate the pretreatment protectant effect of Betaine against possible deleterious effects of florfenicol (FFC) in broiler chicks. A total of 120 broiler chicks were allocated into 4 groups (each of 30 chicks). Group one was kept as a control, while group two was administered with betaine (40mg/kg b.wt. orally from 16 to 20 days of age). Group three was administered with toxic dose of FFC (60 mg/ kg b.wt. orally from 18 to 22 days of age). Group four was co-administered with Betaine with FFC by the same previous doses on the same treatment periods. The biochemical analysis of hepatic and renal indices and histopathological examination were performed to evaluate the protectant effects of betaine on possible FFC-induced hepatorenal toxicity. Results emphasized that large dose of FFC induced hepatorenal toxicity in broilers and pre-treatment with betaine mitigates FFC-adverse effects by enhancement of hepatorenal indices and histopathological pictures of those organs.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77734338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical, Hematological, and Biochemical Alterations Associated With Early and Late Infection of Lumpy Skin Disease in Cattle in Egypt","authors":"A. Saleh, Y. Badr, Osama Abasc, Waleed Aamerd, Yasuo Inoshimab, M. Rahmanb, Hamada Mokhlisf, Ibrahim Abdullazizc","doi":"10.5455/ajvs.104181","DOIUrl":"https://doi.org/10.5455/ajvs.104181","url":null,"abstract":"This study aimed to investigate clinical, hematological, and biochemical alterations associated with early (during the first week.) and late (after one month) of lumpy skin disease (LSD) infection in cattle infected during an outbreak of LSD in Egypt. Animals which were clinically examined directly after the clinical onset of LSD showed, firm elevated skin nodules that were distributed all over the body, fever (more than 40℃) associated with anorexia, general weakness, reduced milk yield, enlargement of superficial lymph nodes, and edema in the brisket and/or limbs. Examination of the affected animals one month after the disease onset revealed marked weight loss, shrunk of skin nodules and formation of sit fast. There were significant increases in body temperature, pulse and respiratory rate in all animals infected with LSD with significant decrease of ruminal movement compared to control group. DNA extracted from skin samples collected from LSD infected animals showed bands at the expected size (172 base pairs) by partial amplification of viral attachment protein using PCR. Reduced RBCS count, Hb content, HCT, and MCHC values with a significant increase in MCV in all LSD infected cows compared to the control group, while leukogram analysis revealed leucopenia and lymphopenia during the 1st week. Post-infection, however, one month post-infection revealed granulocytic leukocytosis. In addition, all LSD infected cows showed inflammatory thrombocytopenia. Animals during early LSD infection showed significant reduction in total protein, albumin, glucose and TAC and significant increase in ALT, AST, ALP, LDH, CPK, C-troponin-1, total and direct bilirubin, creatinine, K and MDA levels compared to the control group. While animals during late infection showed significant decrease in glucose, ALT, AST, LDH, CPK, C-troponin-1, direct bilirubin, K and MDA levels and significant increase in total protein, albumin and TAC compared to the early infection group. It can be concluded that, LSD infection in cattle revealed critical alterations in some hematological and serum biochemical parameters during both early and late stages of infection with negative impact on different organ functions including skin, muscle, liver, kidney and heart.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80863822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological Assessment of Infested Farmed Clarias Gariepinus at Various Developmental Stages in Different Culture Management System","authors":"Olumide Okunade, G. Oladosu, E. Ajani, J. Adejinmi, O. Adeogun","doi":"10.5455/ajvs.129846","DOIUrl":"https://doi.org/10.5455/ajvs.129846","url":null,"abstract":"ABSTRACT: Parasitic infections infringe on different organs causing varying degrees of histological changes which contribute directly or indirectly to fish loss. Histological alterations play significant implication in fish health thereby proffer supports for effective diagnoses to improve the management of cultured fish. Various developmental stages of farmed Clarias gariepinus were randomly collected from 100 farms across the three agricultural zones (East, West and Far – East) in Lagos State, Nigeria from purposively classified culture systems based on water usage management systems (WUMS) into daily water renewal (DWR), weekly water renewal (WWR) and bi – weekly water renewal (BWR)). The organs (skin, gills and intestines) were selected for parasitological examinations and histological sectioning using standard procedure. The water qualities monitored were temperature, dissolved oxygen, pH, ammonia, nitrite, iron, alkalinity, hardness and turbidity and measured using standard methods. Descriptive statistics such as frequency count, percentages, standard deviation were used to determine the occurrence and rate of histological changes in relations with parasitic infections while semi-quantitative analysis was used to assess the histological lesion on gills. This study reveals possible relationship between parasitic infections and histological changes on the gills. The protozoans (Trichodina sp, Vorticella sp., Tetrahymena sp., Chilodonella sp., Piscinoodinium sp. and Ichthyobodo sp.) and monogenean trematode (Dactylogyrus sp., and Gyrodactylus sp.) were related parasites found with varying degrees of histological changes like hyperplasia of the epithelial cells, lifting of the primary epithelium, sloughing of the epithelium, fusion of the secondary lamellae, oedema of the lamellae, vacuolation of the epithelial cells, swelling of the epithelial cells, diffuse infiltration of lamina propria by neutrophil and necrosis of the secondary lamellae, classified into stages I, II and III. The most frequently observed histopathology in all the culture systems was hyperplasia of the gill lamellae; DWR-86.96%, WWR – 88.1%, BWR – 92.86%, followed by the fusion of the secondary lamellae; DWR-65.22%, WWR–75%, BWR– 78.57%. No histopathological change was observed on the skin and the intestine and lesions were least in the daily water renewal (DWR) culture system. Hence, it can be safely inferred that good management practice in terms of water renewal system should be encouraged to alleviate parasitic infections and minimize organ damage in cultured fish.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88129748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Medical and Economical Efficacy of Some Protocols for Treatment of Postpartum Clinical Endometritis in Holstein Dairy Cows","authors":"Hossam Khalil, Reham Waheeb, Samia Rheem, Gamal Amrawi","doi":"10.5455/ajvs.157185","DOIUrl":"https://doi.org/10.5455/ajvs.157185","url":null,"abstract":"Endometritis is one of the most common postpartum diseases which affect daily cows. It causes severe economic losses through increasing treatment cost, days open, service per conception and decrement in milk yield. This study was conducted to evaluate medical efficacy and economic profitability of some treatment protocols for clinical Endometritis. Forty cycling Holstein dairy cows suffering from Endometritis were divided equally and treated as follow: Group 1: cows with presence of corpus luteum (CL) on their ovaries which received PGF2-α. Group 2: cows with presence of CL received PGF2-α + cephapirin (Metricure®) (500mg cephapirin) by intrauterine infusion. Group 3: cows without presence of CL were treated with Metricure® only. Group 4: cows without presence of CL received 100 ml of Lugol's Iodine solution (0.5%) intra-uterine infusion.The results showed that Group-2 animals recorded the fewest days to complete cure and days open and the highest conception rate when compared to other groups. Hematological result at 10th day post-treatment, showed that all the treatment protocols significantly decreased total leukocytic count, neuytrophils%, lymphocytes% and monocytes% except Group-1. Also, all the treated groups recorded significant decrease in serum level of C-reactive protein (CRP) and inteleukin-6 (IL-6). Concerning economical aspect, treatment protocol of Group-2 was the best. In general, treatment with cephapirine (Metricure®) is the best choice for treatment of clinical endometritis in cattle either in presence or absence of CL.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135447657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy Metal Residues in Some Marketed Fish with a Trial of Decontamination using Lactobacillus rhamnosus","authors":"Zakaria Bayoumi, Osama AbouZeid, A. Edris, R. Shawish","doi":"10.5455/ajvs.147649","DOIUrl":"https://doi.org/10.5455/ajvs.147649","url":null,"abstract":"<jats:p />","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85876177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}