Placenta. Supplement最新文献_第4页

Concentration of pregnancy-associated plasma protein A (PAPP-A) in patients with pre-eclamptic toxaemia. 妊娠相关血浆蛋白A (pap -A)在子痫前期毒血症患者中的浓度

Placenta. Supplement Pub Date : 1981-01-01

K Toop, A Klopper

引用次数: 0

Interaction of trophoblast membranes with lymphocytes and other cells. 滋养层膜与淋巴细胞和其他细胞的相互作用。

Placenta. Supplement Pub Date : 1981-01-01

J A McIntyre, W P Faulk, M J O'Sullivan

{"title":"Interaction of trophoblast membranes with lymphocytes and other cells.","authors":"J A McIntyre, W P Faulk, M J O'Sullivan","doi":"","DOIUrl":"","url":null,"abstract":"Certain proteins present in trophoblast membranes may be instrumental in assuring the successful cohabitation of allogeneic tissues in the materno-fetal relationship of normal human pregnancy, inasmuch as preparations of trophoblast microvilli can specifically impede the progress of immune recognition reactions, as measured by the mixed lymphocyte culture (MLC) reaction. Microvilli prepared from fresh placentae were extracted with 3 mol/1 KCl and solubilized overnight in 1 per cent sodium deoxycholic acid (DOC). Following ultracentrifugation, the DOC supernatant chromatographed on Bio-Gel P-200 yielded two major protein peaks. The first peak (P1) was observed to eliminate MLC reactivity, impede the spontaneous turnover of T-cell-enriched populations and interfere with the proliferation of the Daudi lymphoblastoid B cell line. Sepharose CL-6B chromatography of KCl-extracted, DOC-solubilized microvilli yielded a broad peak (m.w. 5 X 10(4) to 5 X 10(5)); 5 micrograms of this specifically abrogated all MLC reactivity while leaving lymphocyte responses to lectins unaffected. The composition of the MLC-inhibitory peak was analysed by using SDS polyacrylamide gel electrophoresis under reducing conditions; it revealed 15 protein bands, six of which also stained for glycoproteins.","PeriodicalId":79246,"journal":{"name":"Placenta. Supplement","volume":"3 ","pages":"95-102"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17287875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracorporeal perfusion of the whole human placenta--a new model. 人全胎盘体外灌注——一种新模型。

Placenta. Supplement Pub Date : 1981-01-01

D Maulik, S F Contractor, J Lippes, A Knight

引用次数: 0

Absence of high-affinity binding of progesterone (R 5020) in human placenta and fetal membranes. 在人胎盘和胎膜中缺乏高亲和力结合的黄体酮(r5020)。

Placenta. Supplement Pub Date : 1981-01-01

P D McCormick, A J Razel, T C Spelsberg, C B Coulam

{"title":"Absence of high-affinity binding of progesterone (R 5020) in human placenta and fetal membranes.","authors":"P D McCormick, A J Razel, T C Spelsberg, C B Coulam","doi":"","DOIUrl":"","url":null,"abstract":"Increased levels of maternal serum progesterone occur during the last stages of human gestation. The function of the high level of this steroid is unknown. The presence of a progesterone receptor in the placenta was investigated to determine whether progesterone action on the placenta might serve as one function of the high level of this steroid. Cytosol and nuclear fractions, derived from human placentae and fetal membranes, were examined for the presence of progesterone receptors by conducting exchange assays, using tritiated R 5020 (17,21-dimethyl-19-norpregna-4,9-diene-3,20-dione) as the radiolabelled ligand. High-affinity, low-capacity binding, characteristic of steroid receptors, was estimated as the difference between binding of radiolabelled ligand in the presence of no unlabelled ligand and that in the presence of a 100-fold excess of unlabelled ligand. These exchange assays were conducted during a 24-hour period at 0 degrees C, to allow maximal stability of the receptor, and during a 3-hour period at 20 degrees C, to allow the rapid exchange of radiolabelled ligand for any bound endogenous progesterone. The assays of all fractions showed no specific binding of the R 5020, thus indicating the absence of progesterone receptors in the cytosol and nuclei of the human placenta and the fetal membranes.","PeriodicalId":79246,"journal":{"name":"Placenta. Supplement","volume":"3 ","pages":"123-32"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18013874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acetylcholine in human term placenta: tissue levels in intact fragments after inhibition in vitro of choline acetyltransferase and relationship to [14C]alpha-aminoisobutyric acid uptake. 人足月胎盘中的乙酰胆碱:体外抑制胆碱乙酰转移酶后完整片段的组织水平及其与[14C] α -氨基异丁酸摄取的关系

Placenta. Supplement Pub Date : 1981-01-01

F Welsch, W C Wenger, D B Stedman

引用次数: 0

Trophoblast and extraembryonic membranes in the immunobiology of human pregnancy. 滋养细胞和胚胎外膜在人妊娠免疫生物学中的作用。

Placenta. Supplement Pub Date : 1981-01-01

W P Faulk

引用次数: 0

Alpha subunit in gestational trophoblastic disease. 妊娠滋养细胞疾病中的α亚基。

Placenta. Supplement Pub Date : 1981-01-01

E I Kohorn, B V Caldwell, J M Cortes