Annales De Biologie Animale Biochimie Biophysique最新文献_第5页

Patterns of gene activity during ovum formation in the mouse 小鼠卵子形成过程中的基因活动模式

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790902

G. Moore, S. Lintern-moore

{"title":"Patterns of gene activity during ovum formation in the mouse","authors":"G. Moore, S. Lintern-moore","doi":"10.1051/RND:19790902","DOIUrl":"https://doi.org/10.1051/RND:19790902","url":null,"abstract":"We have examined the transcriptional activities of the mouse oocyte genome during follicle growth in order to obtain information on the stages at which the synthesis of particular RNA species becomes important. The effects of gonadotrophins on these patterns of transcription have also been investigated. RNA synthesis in oocytes was measured at different stages of growth using a cytochemical application of the standard biochemical assay for DNA-dependent RNA polymerase. The presence of endogenous nucleolar and nucleoplasmic polymerases was detected by autoradiography and their relative activities were quantitated by grain counts. Both activities were relatively low in oocytes of the resting pool of small follicles but increased as growth commenced, reaching a peak as the oocytes approached maximum size. Thereafter RNA polymerase activities declined rapidly and were very low in the oocytes of large, antral follicles. Administration of PMSG to mice had no significant effect on the polymerases of oocytes of small follicles or during the early stages of follicles growth when compared with saline-treated controls. However, in oocytes which had attained maximum size, a statistically significant increase in nucleolar labelling was detected after PMSG treatment. The elevated levels of RNA polymerase activity were confined to oocytes within this growth compartment. These observations are discussed in relation to the way the production of gene transcripts is regulated during the growth of the oocyte, both with respect to the storage of informational molecules and the development of meiotic maturation competence.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"56 1","pages":"1409-1417"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77906708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Early effects of undernutrition on the development of cerebellar Bergmann glia cells 营养不良对小脑伯格曼神经胶质细胞发育的早期影响

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790204

J. Clos, C. Legrand, J. Legrand

引用次数: 5

Diagnosis of the viability of early bovine embryos by fluorescence microscopy 荧光显微镜对早期牛胚胎活力的诊断

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790924

E. Schilling, D. Smidt, B. Sacher, D. Petac, S. Kaschab

引用次数: 27

Etude in vivo chez le rat de l'influence du glucose et du fructose sur le métabolisme intestinal et hépatique 葡萄糖和果糖对大鼠肠道和肝脏代谢影响的体内研究

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790603

E. Gueux, C. Demigné, C. Rémésy

{"title":"Etude in vivo chez le rat de l'influence du glucose et du fructose sur le métabolisme intestinal et hépatique","authors":"E. Gueux, C. Demigné, C. Rémésy","doi":"10.1051/RND:19790603","DOIUrl":"https://doi.org/10.1051/RND:19790603","url":null,"abstract":"Summary. Influence of glucose and fructose on intestinal and hepatic metabolism in the rat in vivo. Intestinal and hepatic metabolism has been studied in rats fed 12-day diets comprising 74 p. 100 of either glucose or fructose. Food was available during 8 hrs, and tissue was sampled on anesthetized animals 6 hrs after the beginning of food intake. In the digestive tract, fructose was poorly converted into glucose (less than 10 p. 100), and enterocyte lactate production was similar with both hexoses, representing less than 10 p. 100 of the absorbed carbohydrates. About 50 p. 100 of the absorbed glucose was taken up by the liver, as against more than 90 p. 100 of the fructose. Considering the transformation of fructose into glucose, the hepatic carbohydrate balance was very similar with the two diets in our sampling condi- tions. Decreased liver uptake of lactate accounted for the increased lactataemia with the fructose diet. This fact was possibly due to an increase of hepatic triose phosphate and pyru- vate production, as fructose was mainly utilized in the fructose-1 phosphate pathway. Depletion of the hepatic ATP, induced by acute administration of fructose, was not observed in our adapted rats. As glycogen storage was more rapid with the fructose diet, triglyceride synthesis (an alternative pathway for the utilization of carbohydrates) certainly increased. This fact could account for the higher plasma triglycerides with the fructose diet, possibly associated with a lower response to the circulating insulin.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"31 1","pages":"715-721"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80617955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Activités de la musculature gastro-intestinale et mouvements des contenus digestifs 胃肠道肌肉的活动和消化内容物的运动

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790625

J. Laplace, C. Roman

引用次数: 11

Effet d'une interruption et de restitutions partielles du recyclage entéro-hépatique sur le niveau de l'excrétion biliaire chez le porc 肠肝循环中断和部分恢复对猪胆汁排泄水平的影响

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790309

Catherine Juste, T. Corring, Georgette Fredou, A. Roger, A. V. Hecke

引用次数: 3

lodohormones in the serum of chick embryos and post-hatching chickens as influenced by incubation temperature. Relationship with the hatching process and thermogenesis 鸡胚和孵化后鸡血清中lodohormone激素与孵育温度的关系。与孵化过程和产热的关系

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19791008

E. Decuypere, E. Nouwen, E. Kuhn, R. Geers, H. Michels

{"title":"lodohormones in the serum of chick embryos and post-hatching chickens as influenced by incubation temperature. Relationship with the hatching process and thermogenesis","authors":"E. Decuypere, E. Nouwen, E. Kuhn, R. Geers, H. Michels","doi":"10.1051/RND:19791008","DOIUrl":"https://doi.org/10.1051/RND:19791008","url":null,"abstract":"temperatures in a forced-draught laboratory incubator. A low incubation temperature resulted in a longer incubation period, whereas eggs incubated at a higher temperature hatched sooner. In all the temperature groups, serum T3 and T4 levels increased during the incubation period studied. Whatever the total duration of incubation within the experimental conditions, maximal serum T3 and Tg levels were always obtained the day of pipping. Embryos having perforated the air-space membrane the day before pipping showed elevated serum T3, but not T4, levels as compared to embryos without perforation. The presence of high serum T3 levels in chick embryos after perforation of the air-space membrane, and the sharp increase in the T3/, ratio before pipping were indicative of the important role of T 3 in the processes of pipping and hatching. After days 16 to 17, depending on the incubation temperature, a plateau for heat production (measured by indirect calorimetry) was reached while serum T3 and T4 levels were still increasing. Following the event of pipping, there was a rapid increase in heat production. A plateau might be due to the physical impossibility of each embryo to react upon an increase in T3 and T4 secretion by an increment in oxygen consumption, and would not exclude a relation between iodohormone levels and thermogenesis during development.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"24 1","pages":"1713-1723"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81588480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 57

Effets de la malnutrition protéinocalorique et de la réalimentation équilibrée sur les activités lipasique et phospholipasique A2 du pancréas et du suc pancréatique de rat 蛋白质热营养不良和均衡再喂养对大鼠胰腺和胰液脂质和磷脂A2活性的影响

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790623

J. Prost, Mahmoud-Nazih Bouziane, J. Belleville

引用次数: 0

Variable development of superovulated bovine embryos between day 6 and day 12 牛超排卵胚胎在第6天至第12天的不同发育

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790920

J. Renard, Y. Heyman

{"title":"Variable development of superovulated bovine embryos between day 6 and day 12","authors":"J. Renard, Y. Heyman","doi":"10.1051/RND:19790920","DOIUrl":"https://doi.org/10.1051/RND:19790920","url":null,"abstract":"The relations between the morphological aspect of superovulated embryos at a given day of recovery and their viability in vitro were studied in order to compose a test for estimating embryo quality. The observation of 994 embryos produced by 100 donors showed that at each day of recovery, from D 6 to D 10, the embryos were distributed into at least four classes according to morphological aspect. At D 11 and D 12 more than 95 p.100 of them were divided into only two classes : abnormal and hatched blastocysts. When they were in the zona pellucida on the day of recovery, 60 p. 100 or more of the apparently normal embryos hatched after culture in vitro. 26 p. 100 or less hatched in vitro when development was apparently retarded, and none of those having an abnormal aspect, according to our criteria, hatched. When they were already hatched from the zona pellucida at recovery, 80 p. 100 of the embryos showed an increase in size after 24 hrs. of culture in vitro. The percentage of normal embryos decreased from D 6 to D 7, and then remained stable until at least D 10. The percentage of retarded embryos increased from D 6 to D 7 and then decreased. A few retarded embryos were visible at days 11 and 12. The percentage of degenerate embryos increased from D 7 to D 8 and remained stable afterwards. The proportion of donors with more than 50 p. 100 of degenerate embryos increased from D 6 to D 12. The relation with development in vivo after transplantation has been discussed.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"31 1","pages":"1589-1598"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89531419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Mechanisms governing onset of ovarian cyclicity at puberty in the lamb 羔羊青春期卵巢周期的发病机制

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790821

D. Foster, K. Ryan

引用次数: 37