Analytical Chemistry Research最新文献_第4页

Quantitative measurement of metal chelation by fourier transform infrared spectroscopy 傅里叶变换红外光谱法定量测定金属螯合作用

Analytical Chemistry Research Pub Date : 2015-12-01 DOI: 10.1016/j.ancr.2015.10.002

Monika E. Miller, Lani P. McKinnon, Edward B. Walker

引用次数: 9

Expanded metabolite coverage of Saccharomyces cerevisiae extract through improved chloroform/methanol extraction and tert-butyldimethylsilyl derivatization 通过改进氯仿/甲醇萃取和叔丁基二甲基硅基衍生化，扩大了酿酒酵母提取物代谢物的覆盖范围

Analytical Chemistry Research Pub Date : 2015-12-01 DOI: 10.1016/j.ancr.2015.10.001

Sakda Khoomrung , Jose L. Martinez , Stefan Tippmann , Suwanee Jansa-Ard , Marieke F. Buffing , Raffaele Nicastro , Jens Nielsen

{"title":"Expanded metabolite coverage of Saccharomyces cerevisiae extract through improved chloroform/methanol extraction and tert-butyldimethylsilyl derivatization","authors":"Sakda Khoomrung , Jose L. Martinez , Stefan Tippmann , Suwanee Jansa-Ard , Marieke F. Buffing , Raffaele Nicastro , Jens Nielsen","doi":"10.1016/j.ancr.2015.10.001","DOIUrl":"10.1016/j.ancr.2015.10.001","url":null,"abstract":"<div><p>We present an improved extraction and derivatization protocol for GC–MS analysis of amino/non-amino acids in <em>Saccharomyces cerevisiae</em>. Yeast cells were extracted with chloroform: aqueous-methanol (1:1, v/v) and the resulting non-polar and polar extracts combined and dried for derivatization. Polar and non-polar metabolites were derivatized using <em>tert</em>-butyldimethylsilyl (<em>t</em>-BDMS) dissolved in acetonitrile. Using microwave treatment of the samples, the derivatization process could be completed within 2 h (from >20 h of the conventional method), providing fully derivatized metabolites that contain multiple derivatizable organic functional groups. This results in a single derivative from one metabolite, leading to increased accuracy and precision for identification and quantification of the method. Analysis of combined fractions allowed the method to expand the coverage of detected metabolites from polar metabolites i.e. amino acids, organic acids and non-polar metabolites i.e. fatty alcohols and long-chain fatty acids which are normally non detectable. The recoveries of the extraction method was found at 88 ± 4%, RSD, N = 3 using anthranilic acid as an internal standard. The method promises to be a very useful tool in various aspects of biotechnological applications i.e. development of cell factories, metabolomics profiling, metabolite identification, <sup>13</sup>C-labeled flux analysis or semi-quantitative analysis of metabolites in yeast samples.</p></div>","PeriodicalId":7819,"journal":{"name":"Analytical Chemistry Research","volume":"6 ","pages":"Pages 9-16"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ancr.2015.10.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76220124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Facilitating the indirect detection of genomic DNA in an electrochemical DNA biosensor using magnetic nanoparticles and DNA ligase 利用磁性纳米颗粒和DNA连接酶在电化学DNA生物传感器中促进基因组DNA的间接检测

Analytical Chemistry Research Pub Date : 2015-12-01 DOI: 10.1016/j.ancr.2015.10.004

Roozbeh Hushiarian , Nor Azah Yusof , Abdul Halim Abdullah , Shahrul Ainliah Alang Ahmad , Sabo Wada Dutse

{"title":"Facilitating the indirect detection of genomic DNA in an electrochemical DNA biosensor using magnetic nanoparticles and DNA ligase","authors":"Roozbeh Hushiarian , Nor Azah Yusof , Abdul Halim Abdullah , Shahrul Ainliah Alang Ahmad , Sabo Wada Dutse","doi":"10.1016/j.ancr.2015.10.004","DOIUrl":"10.1016/j.ancr.2015.10.004","url":null,"abstract":"<div><p>A common problem in applying biosensors for the detection of genomic DNA is detecting short sequences in large amounts of long double stranded DNA. A gold electrode modified with a conductive nanocomposite, poly(3,4-ethylene-dioxythiophene), and gold nanoparticles was functionalized with 2,6-Pyridinedicarboxylic acid. Immobilization of a 20-mer DNA probe as the bioreceptor was successfully carried out via a peptide bond on the surface of the modified electrode. Two segments of 15 and 20 base probes were designed and named as Capture and Reporter probes respectively. The 20-mer Reporter probe was complementary to the bioreceptor and the 15-mer Capture probe was designed to bind on to the surface of the iron oxide magnetic nanoparticles. A 35-base Target DNA complementary to the Capture and the Reporter probes was used as Template in the ligation process, with the ligation between the Reporter and Capture probes mediated by T4 ligase. Iron oxide magnetic nanoparticles functionalized with carboxylic groups on their surface synthesized in a new method were attached to the 15-mer Capture probe. After the denaturation of the final ligation product, the separation of the attached probes was carried out using 5 G permanent magnets in a three step washing procedure in TE buffer. The hybridization of the DNA bioreceptor and the Reporter probe attached to the Capture probe-Fe<sub>3</sub>O<sub>4</sub> was monitored via oxidation and reduction of the new redox marker (ruthenium complex) intercalated into the double helix.</p><p>This technique was found to be reliably repeatable. The indirect detection of genomic DNA using this method is significantly improved and showed high efficiency in small amounts of samples with the detection limit of 5.37 × 10<sup>−14</sup> M.</p></div>","PeriodicalId":7819,"journal":{"name":"Analytical Chemistry Research","volume":"6 ","pages":"Pages 17-25"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ancr.2015.10.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78207376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Direct electron transfer biosensor for hydrogen peroxide carrying nanocomplex composed of horseradish peroxidase and Au-nanoparticle – Characterization and application to bienzyme systems 由辣根过氧化物酶和金纳米粒子组成的过氧化氢纳米复合物的直接电子转移生物传感器-表征及其在双酶系统中的应用

Analytical Chemistry Research Pub Date : 2015-09-01 DOI: 10.1016/j.ancr.2015.05.001

Yusuke Okawa, Naoto Yokoyama, Yoshinori Sakai, Fumiyuki Shiba

引用次数: 20

Analytical protocols for the determination of sulphur compounds characteristic of the metabolism of Chlorobium limicola 限定氯藻代谢特征硫化合物测定的分析方法

Analytical Chemistry Research Pub Date : 2015-09-01 DOI: 10.1016/j.ancr.2015.05.002

A. Aliboni , L. Lona , C. Felici , N. Corsaro , G. Izzo , E. De Luca

引用次数: 2

Feasibility study of calibration strategy for direct quantitative measurement of K and Mg in plant material by laser-induced breakdown spectrometry 激光诱导击穿光谱法直接定量测定植物材料中钾、镁的校准策略的可行性研究

Analytical Chemistry Research Pub Date : 2015-09-01 DOI: 10.1016/j.ancr.2015.06.003

Daniel Menezes Silvestre, Felipe Miranda Barbosa, Bruno Teves Aguiar, Flávio Oliveira Leme, Cassiana Seimi Nomura

{"title":"Feasibility study of calibration strategy for direct quantitative measurement of K and Mg in plant material by laser-induced breakdown spectrometry","authors":"Daniel Menezes Silvestre, Felipe Miranda Barbosa, Bruno Teves Aguiar, Flávio Oliveira Leme, Cassiana Seimi Nomura","doi":"10.1016/j.ancr.2015.06.003","DOIUrl":"10.1016/j.ancr.2015.06.003","url":null,"abstract":"<div><p>The calibration and quantitative measurement is the “<em>Achilles heel</em>” of the LIBS technique. This paper deals with a method developed for the direct measurement of K and Mg in plant samples. Instrumental parameters were optimized and the best condition found was a 50μm spot size, 10Hz laser repetition rate, 75 accumulated laser pulses with 25mJ/pulse and 0.25μs of delay time. For method calibration, the use of synthetic standard calibrating material prepared by the addition of increasing concentrations of K and Mg in wood, filter paper and babassu mesocarp was proposed in order to assess the feasibility of using these various matrices in plant samples analysis. The limits of detection of proposed method were 2–30 and 6–27μgg<sup>−1</sup> for K and Mg, respectively. The use of the carbon emission wavelength at 247.856nm was used as internal standard to improve the analytical results. Certified reference materials of plants were used to check the accuracy of the proposed method and recovery around 82% and 100% were obtained in all cases.</p></div>","PeriodicalId":7819,"journal":{"name":"Analytical Chemistry Research","volume":"5 ","pages":"Pages 28-33"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ancr.2015.06.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82168348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Amperometric detection of carbohydrates based on the glassy carbon electrode modified with gold nano-flake layer 基于金纳米薄片修饰的玻碳电极的碳水化合物安培检测

Analytical Chemistry Research Pub Date : 2015-09-01 DOI: 10.1016/j.ancr.2015.06.001

Huy Du Nguyen , T. Thuy Luyen Nguyen , Khac Manh Nguyen , Anh Mai Nguyen , Quoc Hien Nguyen

引用次数: 3

Optical sensing of 3-phenoxybenzoic acid as a pyrethroid pesticides exposure marker by surface imprinting polymer capped on manganese-doped zinc sulfide quantum dots 锰掺杂硫化锌量子点表面印迹聚合物对3-苯氧苯甲酸作为拟除虫菊酯农药暴露标记物的光学传感

Analytical Chemistry Research Pub Date : 2015-09-01 DOI: 10.1016/j.ancr.2015.06.002

Vivek Pandey , Abhishek Chauhan , Gajanan Pandey , Mohana Krishna Reddy Mudiam

{"title":"Optical sensing of 3-phenoxybenzoic acid as a pyrethroid pesticides exposure marker by surface imprinting polymer capped on manganese-doped zinc sulfide quantum dots","authors":"Vivek Pandey , Abhishek Chauhan , Gajanan Pandey , Mohana Krishna Reddy Mudiam","doi":"10.1016/j.ancr.2015.06.002","DOIUrl":"10.1016/j.ancr.2015.06.002","url":null,"abstract":"<div><p>The present communication deals with the synthesis of luminescent Mn-doped ZnS quantum dots (QDs) anchored to surface imprinted polymer for the optical sensing of 3-phenoxy benzoic acid (3-PBA) in urine samples. The combination of sensing and surface functionalization not only improves the selectivity of the method, but also increases the optosensing ability of the material for non-phosphorescent substances. The developed material was utilized for the selective and sensitive detection of 3-PBA in urine samples. The proposed method shows good linearity with a regression coefficient (<em>R</em><sup>2</sup>) of 0.98. The limit of detection was found to be 0.117μM. The method has an acceptable precision and accuracy which are found to be less than 8% and 80–90% respectively at three different concentrations. The quenching constant of quantum dot-molecular imprinted polymer was found to be 3.4 times higher to that of the quantum dot-non imprinted polymer (QD-NIP) as calculated by Stern–Volmer equation. The sensing method developed has shown immense utility to detect 3-PBA in complex biological samples like urine.</p></div>","PeriodicalId":7819,"journal":{"name":"Analytical Chemistry Research","volume":"5 ","pages":"Pages 21-27"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ancr.2015.06.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80039423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Stripping voltammetric detection of nephrotoxic drug cefitizoxime in wastewater 溶出伏安法检测废水中肾毒性药物头孢替肟

Analytical Chemistry Research Pub Date : 2015-06-01 DOI: 10.1016/J.ANCR.2015.03.002

J. Rather, R. Jain

引用次数: 5

Stripping voltammetric detection of nephrotoxic drug cefitizoxime in wastewater 溶出伏安法检测废水中肾毒性药物头孢替肟

Analytical Chemistry Research Pub Date : 2015-06-01 DOI: 10.1016/j.ancr.2015.03.002

Jahangir Ahmad Rather , Rajeev Jain

{"title":"Stripping voltammetric detection of nephrotoxic drug cefitizoxime in wastewater","authors":"Jahangir Ahmad Rather , Rajeev Jain","doi":"10.1016/j.ancr.2015.03.002","DOIUrl":"https://doi.org/10.1016/j.ancr.2015.03.002","url":null,"abstract":"<div><p>The objective of the present work is to develop the stripping voltammetric method for determination of nephrotoxic drug cefitizoxime in pharmaceutical formulation and its application to wastewater analysis. Solubilized system of different surfactants viz. cationic, anionic and non-ionic influences the electrochemical response of cefitizoxime. Solubilized system of CTAB containing cefitizoxime enhanced the peak current while anionic and non-ionic showed an opposite effect. The current signal due to the reduction process is a function of concentration of the cefitizoxime, pH of medium, type of surfactant and accumulation time at electrode surface. The proposed SWCAdSV (Squarewave Cathodic Adsorptive Voltammtery) and DPCAdSV (Differential Pulse Cathodic Adsorptive Voltammetry) are linear over concentration range 1.732–6.901μg/mL and 4.792–30.672μg/mL with detection limit of 0.76ng/mL and 2.63ng/mL, respectively. The method is successfully applied for determination of cefitizoxime in pharmaceutical formulation and wastewater with mean percentage recovery of 99.73% and 98.51%, respectively.</p></div>","PeriodicalId":7819,"journal":{"name":"Analytical Chemistry Research","volume":"4 ","pages":"Pages 13-19"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ancr.2015.03.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90029922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5