Analytical Chemistry Letters最新文献

{"title":"High-Performance Thin Layer Chromatography Method for Simultaneous Estimation of Water-Soluble Vitamins: Analytical Method Development, Validation and Applications","authors":"Mukesh N. Kher, S. Dholakia, Grisha G. Shah, D. Sureja, Vaibhav Bhatt, D. Sheth","doi":"10.1080/22297928.2022.2140072","DOIUrl":"https://doi.org/10.1080/22297928.2022.2140072","url":null,"abstract":"Abstract Vitamins are pivotal ingredients in dietary supplements and nutraceutical products. Since many vitamins are unstable and rapidly degraded, it’s crucial to keep track of their loss throughout processing and storage. Monitoring hydrophilic vitamins may be more crucial than monitoring those lipophilic vitamins because the body does not maintain them as well. There seems to be a need for an all-encompassing method to estimate hydrophilic vitamins. In this study, we have developed a high-performance thin layer chromatography method for estimation of four water-soluble vitamins simultaneously using HPTLC silica-gel 60GF254 plates and ethyl acetate: methanol: hydrochloric acid (0.1N) (8.5:1:0.5 v/v/v) as a mobile phase, which was successfully validated as per the ICH guideline. All the validation parameters were within the acceptable range established by the ICH guideline. The method is precise, reproducible, easy, reliable, and applicable for the estimation of water-soluble vitamins in marketed products. To best of our knowledge, it is simpler, less time-consuming, and more economical than other approaches that have been published for the same purposes GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"45 1","pages":"576 - 585"},"PeriodicalIF":0.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89728140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and HPLC Method Validation for Determination of Dopamine Hydrochloride in Prepared Nano Particles and Pharmacokinetic Application","authors":"S. Neha, A. Mishra, Laxmi Rani, Suraj P. Verma, P. K. Sahoo","doi":"10.1080/22297928.2022.2121662","DOIUrl":"https://doi.org/10.1080/22297928.2022.2121662","url":null,"abstract":"Abstract The emotional control monoamines neurotransmitter catecholamine group of chemicals is dopamine. It has been described and recognized that a lack of dopamine containing neurons contributes to neurological issues, such as Parkinsonism. Due to the incapability of dopamine HCl to cross the blood brain barrier, the advanced novel drug delivery of dopamine HCl is performed in the form of nano structure lipid carrier system (NLC-DOPA) to bypass the blood brain barrier using intranasal route of administration with improved compliance bioavailability and less side effect. The NLC-DOPA was prepared by the high pressure homogenizer ultra-probe sonicator and characterized by the particle size analyzer, zeta potential, transmission electron microscopy (TEM) and X-Ray diffraction (XRD). The determination of dopamine hydrochloride in formulation was evaluated by drug entrapment efficiency further it is validated by analytical method development using high performance liquid chromatography (HPLC) with C18 ODS column and 0.05 M KH2PO4 buffer pH 2.5 and Methanol (90:10) along with retention time 4.872 min at 280 nm wavelength. The method was validated for dopamine HCl in formulation with linearity, precision and recovery analysis. The study was shown that method was easy, rapid, accurate, specific and precise for quality analysis of dopamine HCl in NLC-DOPA formulation. The validated method of HPLC was further used for the biological application in term of the pharmacokinetic study to find out the concentration of drug in blood as well as brain. The pharmacokinetic parameters were calculated with help of the software PK solver 2.0 to determine the maximum concentration (Cmax), Maximum time (Tmax), Elimination half life (t1/2), Area under the curve (AUC0-t), volume of distribution (Vz/F_obs) and Clearance (Cl/F_obs) of all groups of animals (n=3). The study showed that the NLC-DOPA was producing a sustained and controlled release in appropriate amount of drug through intranasal bypass delivery into brain. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"41 1","pages":"528 - 541"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75315037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Investigation of Volatile Organic Compounds of Cattle Rumen Metabolites using HS-SPME and PoraPak-Q Odor Trapping Methods","authors":"Victor O. Omondi, Geoffrey O. Bosire, J. Onyari, M. N. Getahun","doi":"10.1080/22297928.2022.2100276","DOIUrl":"https://doi.org/10.1080/22297928.2022.2100276","url":null,"abstract":"Abstract In the rapidly increasing field of metabolomic research, fast and accurate trapping of volatile odor compounds from biological samples is critical. Here a comparative evaluation of HS-SPME and PoraPak-Q adsorbent odor trapping methods followed by GC-MS analysis were used to determine volatile compounds in cattle rumen. Compared to the PoraPak-Q adsorbent, the HS-SPME method was more effective in trapping diverse metabolites including low molecular weight and highly volatile compounds such as carbon dioxide, acetic and butyric acid. Additionally, using the HS-SPME method, shorter trapping times were achieved (30 minutes) whilst the PoraPak-Q adsorbent required longer time extending beyond 1 hour for effective volatile trapping. In the context of metabolomics analysis from biological samples, the two different methods vary in determination of chemo-diversities, qualitatively and abundance of shared odor, and time required to trap odors, which are critical in such studies. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"21 1","pages":"451 - 459"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73679372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of Rapid, Timesaving, and Cost-effective UHPLC Method for Simultaneous Quantification of Cinnarizine, its Five Specified Impurities, Two Degradation Products and Two Antioxidants","authors":"D. Mhaske, A. Kumbhar","doi":"10.1080/22297928.2022.2108721","DOIUrl":"https://doi.org/10.1080/22297928.2022.2108721","url":null,"abstract":"Abstract This article explains the developed and validated rapid, timesaving, and cost-effective UHPLC method for simultaneous quantification of cinnarizine, its five specified impurities (Impurity-A to E), two degradation products (cinnamyl piperazine and benzhydrol), and two antioxidants (methylparaben and propylparaben). Furthermore, when coupled with a mass spectrometer, the proposed method provides additional advantages for confirmation of results and correct identification based on molecular weight. All analytes were eluted within 15 minutes on an ACQUITY, UPLC, BEH C18 (150 mm x 2.1 mm, 1.7 µm) column at 40.0°C by using two mobile phases containing different compositions of 10 mM ammonium acetate, acetonitrile, and acetic acid in gradient elution mode. The linearity curves of cinnarizine, its impurities, and degradation products showed good results in a correlation coefficient of 0.999 with a lower detection limit (0.1125 μg/ mL) and quantification limit (0.1875 μg/mL) at 230 nm. A forced degradation study on spiked and unspiked solutions proved their specificity with improvements and their significance. This proposed method involves a lower flow rate (0.35 mL/min.) with a shorter run time, which provides faster analysis, reduces wastage, reduces the cost, and specifies the greener advantages. The outcome of the validation as per ICH guidelines proved that the proposed UHPLC method is accurate, precise, and timesaving for simultaneous quantification of all analytes in active pharmaceutical ingredient, tablets, capsules, and oral suspension of cinnarizine. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"35 1","pages":"488 - 504"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72684520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Impurities in Graphite Using Proton Induced Gamma Ray Emission, Total Reflection X-ray Fluorescence and Instrumental Neutron Activation Analysis","authors":"M. Ghosh, T. Chavan, G. Reddy, Remya Devi P.S, S. Kumar, K. K. Swain","doi":"10.1080/22297928.2022.2108722","DOIUrl":"https://doi.org/10.1080/22297928.2022.2108722","url":null,"abstract":"Abstract Determination of impurities in graphite is very important for its quality control, as their presence even at trace level can affect the performance of graphite in various applications. Graphite with equivalent boron content (EBC) less than 5 mg kg-1 is considered as nuclear grade. Elements with high neutron absorption cross section (boron and rare earths) contribute significantly to EBC. Non-destructive method is preferred as there is no sample processing and probability of loss of volatile elements while digestion. Proton Induced Gamma Ray Emission (PIGE), Instrumental Neutron Activation Analysis (INAA) were utilized for the non-destructive determination of impurities in both nuclear and commercial grade graphite. Low Z elements like Li, B, F, Na, Al and Si were detected in graphite by PIGE whereas Na, K, Sc, Cr, Mn, Fe, Co, Zn, Rb, Zr, Sb, Cs, La, Ce, Nd, Sm, Eu, Tb, Yb, Hf, Ta, Th were determined using INAA. Few elements like Ca, Ti, V, Ni, Sr and Pb remained undetected by both the non-destructive techniques. These elements were determined by Total Reflection X-ray Fluorescence (TXRF) after digestion of the graphite samples by dry ashing. Combinations of these techniques were utilized to get maximum information regarding the impurities present in graphite. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"25 1","pages":"437 - 450"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74259766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Heavy Metal Contaminants in Water and the Environment","authors":"P. Hitaishi, R. Verma, P. Khurana, S. Thatai","doi":"10.1080/22297928.2022.2098815","DOIUrl":"https://doi.org/10.1080/22297928.2022.2098815","url":null,"abstract":"Abstract A simple and highly sensitive plasmonic colorimetric method using nanomaterials is reviewed to detect heavy metal ions in aqueous samples. The basic properties of nanomaterials, which make them behave differently from their bulk counterpart is addressed. The main focus is on the potential applications of intelligent materials like metal NPs and core-shell nanocomposites for the sensing of heavy metal ions in an aqueous media and environment. The article also presents an overview of the science and technology behind nanoparticle-based sensors in general and core-shell sensors in particular. This article embodies an overview of the exciting and emerging field of sensors with an emphasis on metal nanoparticles and core-shell nanocomposites. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"2017 1","pages":"473 - 487"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90654364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Profiling of Averrhoa carambola Fruit Extract using UHPLC-ESI-QTOF-MS and Determination of the Concentration of Essential Elements using MP-AES","authors":"R. Biswal, D. Patnana, V. N. R. K. Vutukuri","doi":"10.1080/22297928.2022.2124125","DOIUrl":"https://doi.org/10.1080/22297928.2022.2124125","url":null,"abstract":"Abstract A rapid and sensitive ultra-high-performance liquid chromatography-electrospray ionization quadrupole time of flight mass spectrometry (UHPLC-ESI-QTOF-MS) based method was developed to separate and identify 44 phenolics and non-phenolics present in Averrhoa carambola fruit in three different stages of its growth. 5 anthocyanins, 9 phenolic acids, 3 flavones, 5 flavanols, 3 flavanones, amino acids, vitamins and other phenolic and non-phenolic compounds were identified. The identification and quantification of 27 bioactive compounds from methanolic star fruit extract are being reported for the first time. All the metabolites were quantified by normalizing the area under the peaks with that of the internal standard hydrocortisone. The extraction of metabolites by using a homogeniser (bead beater) was found to be the best method for the extraction of metabolites. Four essential microelements (Zn, Fe, Mn, Cr) and three macroelements (K, Mg, Na) were quantified from star fruit using MP-AES. The zinc concentration (6.988 mg/100 g) in star fruit was found to be higher than in many cereals, vegetables and fruits. The methanolic star fruit extract showed excellent DPPH scavenging activity of 93% after 30 min of incubation in dark for the dry weight sample concentration of 50 mg/ml. In conclusion, the UHPLC-ESI-QTOF-MS based method developed and MP-AES results presented here can be used in the quality control of food supplements and medicines. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"1 1","pages":"505 - 527"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89312629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synchronous Fluorescence Method for Determination of Bevacizumab and Fluorouracil in Laboratory Prepared Mixture, Pharmaceutical Dosage Forms and Spiked Plasma","authors":"H. Salem, Mohamed A. Mawhoup, M. Sayed, A. Abdelaziz","doi":"10.1080/22297928.2022.2088300","DOIUrl":"https://doi.org/10.1080/22297928.2022.2088300","url":null,"abstract":"Abstract Bevacizumab (BVC) is commonly co-administered with 5-fluorouracil (FLO) as a highly successful treatment for colon cancer. In this study, two spectrofluorimetric techniques were combined to provide an approach for concurrent quantification of BVC and FLO that is very sensitive, quick, easy and accurate. The inclusion of Tween-80 micellar system increased the native fluorescence intensity values of BVC and FLO, while the second derivative of the synchronous fluorescence intensity of the cited drugs at Δλ=100 nm allowed the concurrent estimation of cited drugs. The effect of various experimental conditions on the synchronous fluorescence of cited drugs were extensively examined to optimize them. The second derivative synchronous fluorimetry peak amplitudes for BVC and FLO were recorded at 450 nm and 440 nm, respectively. At a range of 100–1100 and 100–600 ng mL−1, the fluorescence-concentration graphs were built, with smaller quantification limits of 60.0 and 70.0 ng mL−1 and detection limits of 20.0 and 23.0 ng mL−1 for BVC and FLO, respectively. Without any major interference, the technique was effectively used to determine the cited drugs in raw material along with pharmaceutical formulations. The proposed technique was shown to be extremely precise and accurate when related to the reported techniques statistically. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"194 2","pages":"460 - 472"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91503480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of a New Stereoselective RP-HPLC Method for Simultaneous Quantification of Tadalafil, its One Enantiomer, and Two Diastereomers in API and Tablet Form","authors":"D. Mhaske, A. Kumbhar","doi":"10.1080/22297928.2022.2089595","DOIUrl":"https://doi.org/10.1080/22297928.2022.2089595","url":null,"abstract":"Abstract A new, simple, and stereoselective RP-HPLC method was developed and validated for the simultaneous quantification of tadalafil, its one enantiomer, and two diastereomers in API and tablets. Using varied compositions of water, acetonitrile, and acetic acid as mobile phases in gradient mode at a 0.40 mL/min flow rate and detection at 285 nm, all this separation was achieved on the Lux Cellulose-3 (150 mm x 4.6 mm, 3 µm) column at a 30.0°C oven temperature. All isomers were eluted within 24 minutes, with a resolution of more than 2.3 between any two isomers. With 10.0 µL injection volume, the LOD and LOQ were determined to be 0.06 µg/mL and 0.10 µg/mL, respectively. The linearity of tadalafil (0.10-400 µg/mL), one enantiomer, and two diastereomers (0.10-4.0 µg/mL) was confirmed with a correlation coefficient of 0.999. The forced degradation study revealed the specificity for all the peaks as well as the conversion of tadalafil into diastereomers (6S, 12aR) in acidic conditions and into diastereomers (6R, 12aS) in alkaline conditions. At lower concentrations, the recoveries for all isomers ranged from 100.0 ± 15.0%, while the assay values for tadalafil were within 100.0 ± 2.0%. According to the validation outcome as per ICH guidelines, the proposed method is an accurate, precise, linear, and robust stereoselective method for simultaneous quantification. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"5 1","pages":"419 - 436"},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75227148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of Liquid Chromatography and Mass Spectrometry to Identify and Quantify Chemical Components in Tea Extracts","authors":"Diavian Bellamy, Mieka Cobbs, S. Rahhal, A. Bakarr Kanu","doi":"10.1080/22297928.2022.2088299","DOIUrl":"https://doi.org/10.1080/22297928.2022.2088299","url":null,"abstract":"Abstract A fast, straightforward, efficient, and high throughput reverse-phase high-performance liquid chromatography and electrospray ionization mass spectrometry method has been developed to analyze tea extracts containing polyphenols. The polyphenols detected in green tea (GT), black tea (BT), and paradise tropical tea (PTT) were separated in 18 min at wavelengths 254 nm, 260 nm, and 280 nm with a gradient elution on the RP-HPLC system. Our approach detected, identified, and quantified three catechins in the tea extracts analyzed, and the response at 280 nm wavelength was the best. The ESI-MS data confirmed the fragmentation patterns of the catechins detected in the tea extract. The validation data showed that the limit of detection (LOD) and limit of quantitation (LOQ) of catechins ranged from 5.26 ± 0.02 to 36.44 ± 0.02 ppb, and 17.52 ± 0.03 to 121.45 ± 0.16 ppb, respectively, for six catechins studied. The standard addition calibration approach used to quantify the catechin content in the tea extract simultaneously showed that PTT has a higher catechin content than GT and BT. The content of polyphenols in GT, BT, and PTT are summarized. This approach holds great promise for quality control studies to quantify polyphenols in nutritional products. GRAPHICAL ABSTRACT","PeriodicalId":7793,"journal":{"name":"Analytical Chemistry Letters","volume":"431 1","pages":"292 - 301"},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76660489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}