Analytical Letters最新文献

Aggregation Induced Emission Fluorescent Probes for the Determination and Discrimination of Collagen I and II 聚集诱导发射荧光探针测定和鉴别胶原I和II

4区化学

Analytical Letters Pub Date : 2026-07-06 DOI: 10.1080/00032719.2026.2697444

Zhenggang Tan, Gao Y, Binxu Han, Farui Qin, Zhiyin Du, Zhengjun Bian

{"title":"Aggregation Induced Emission Fluorescent Probes for the Determination and Discrimination of Collagen I and II","authors":"Zhenggang Tan, Gao Y, Binxu Han, Farui Qin, Zhiyin Du, Zhengjun Bian","doi":"10.1080/00032719.2026.2697444","DOIUrl":"https://doi.org/10.1080/00032719.2026.2697444","url":null,"abstract":"Rapid and reliable discrimination of collagen subtypes is critical for biomaterials evaluation, tissue engineering, and disease-related extracellular matrix studies. However, this task remains difficult when conventional fluorescence readouts are used. In this study, two tetraphenylethene (TPE)-based aggregation-induced emission (AIE) fluorescent probes, bis[12-(N,N-dimethyl-N′,N′,N′-trimethylethane-1,2-diammonium)dodecyloxy]-substituted tetraphenylethene (TPE-NN) and bis[12-(N-methylimidazolium)dodecyloxy]-substituted tetraphenylethene (TPE-MZ), were developed as environment-sensitive reporters for viscosity and collagen determination. Both probes displayed viscosity-enhanced emission, consistent with restricted intramolecular motion. TPE-NN exhibited a fluorescence turn-on response at 488 nm with a strong linear relationship between emission intensity and medium viscosity (R2 = 0.9936), whereas TPE-MZ showed comparable viscosity responsiveness (R2 = 0.9835). In acidic aqueous media, the emission intensities of both probes increased with the concentrations of collagen types I and II, enabling quantitative collagen determination. However, direct spectral inspection alone was insufficient for robust subtype identification. Therefore, principal component analysis (PCA) was applied to the fluorescence datasets which resulted in clear separation of collagen I and collagen II signatures. TPE-NN produced tighter clustering and more distinct interclass separation than TPE-MZ. Molecular docking provided mechanistic insight into the differential binding behavior underlying subtype discrimination. These results establish a fluorescence-chemometrics strategy for collagen subtype identification and identify TPE-NN as a promising AIE probe for collagen discrimination in complex microenvironments.","PeriodicalId":7788,"journal":{"name":"Analytical Letters","volume":"1 1","pages":"1-18"},"PeriodicalIF":0.0,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148672200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Separation of Succinic Acid and Methylmalonic Acid Using Noncovalent Metal-Cyclodextrin Complexation and Ion Mobility-Mass Spectrometry 非共价金属-环糊精络合-离子迁移-质谱法分离琥珀酸和甲基丙二酸

4区化学

Analytical Letters Pub Date : 2026-03-27 DOI: 10.1080/00032719.2026.2643890

Binghao Jiao, Jiacheng Ye, Jingchang Tian, Yuting Yan, Yongyao Wu, Chuan-Fan Ding

引用次数: 0

Nanoenzyme-Based Colorimetric Assay for Rapid Glutathione Determination Using Microwave-Synthesized Palladium Nanocrystals 微波合成钯纳米晶纳米酶比色法快速测定谷胱甘肽

4区化学

Analytical Letters Pub Date : 2026-02-05 DOI: 10.1080/00032719.2026.2624698

Quanxiu Wang, Zijin Chen, Jiaoyan Gao, Xingshuo Wang, Xinlei Huang, Yuting Shen, Hui Lu, Leilei Guo

引用次数: 0

High Affinity Antibodies for the Development of a Specific Immunoassay for the Determination of Bongkrekic Acid in Food 高亲和抗体用于食品中苯丙酸的特异性免疫测定

4区化学

Analytical Letters Pub Date : 2026-01-20 DOI: 10.1080/00032719.2026.2615056

Shibei Shao, Li Nan, Rui Liu, Yingjie Zhang, Yantong Pan, Kai Wen, Xuezhi Yu, Jianzhong Shen, Zhanhui Wang

引用次数: 0

Cu/Zn–ZIF: a Metal–Organic Framework for Eliminating Excessive Stimulation from Cyclic Adenosine Monophosphate (cAMP) Cu/ Zn-ZIF：消除环磷酸腺苷（cAMP）过度刺激的金属-有机框架

4区化学

Analytical Letters Pub Date : 2026-01-13 DOI: 10.1080/00032719.2025.2609112

Lingchun Wei, Shiyi Chen, Hongbo Zhao, Shixin Xu, Ganghui Chu

{"title":"Cu/Zn–ZIF: a Metal–Organic Framework for Eliminating Excessive Stimulation from Cyclic Adenosine Monophosphate (cAMP)","authors":"Lingchun Wei, Shiyi Chen, Hongbo Zhao, Shixin Xu, Ganghui Chu","doi":"10.1080/00032719.2025.2609112","DOIUrl":"https://doi.org/10.1080/00032719.2025.2609112","url":null,"abstract":"Cyclic adenosine monophosphate (cAMP) is a ubiquitous second messenger that orchestrates metabolism, gene transcription and neuronal signaling. Sustained overactivation of cAMP pathways, however, is implicated in neurodegenerative, cardiovascular and inflammatory disorders. Conventional regulation mainly relies on phosphodiesterase (PDE) inhibitors, which raise intracellular cAMP and may aggravate adverse effects when used in complex physiological environments. Here, a bimetallic zeolitic imidazolate framework, Cu/Zn-ZIF, was constructed using 2-methylimidazole as the ligand to achieve controlled hydrolysis of cAMP under near-physiological conditions. The material was synthesized by a mild water-bath method and comprehensively characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FT-IR) spectroscopy and high-performance liquid chromatography (HPLC). Under neutral pH at 37 °C, 10.0 mg of Cu/Zn-ZIF decreased the cAMP concentration in fetal bovine serum by 26.8%, effectively attenuating excessive stimulation while avoiding complete depletion of the messenger. The catalyst retained activity in cAMP-rich jujube extracts and serum, demonstrating good stability and tolerance to common interfering species in complex matrices. Kinetic analysis confirmed its nanozyme-like behavior, and the self-limited conversion was favorable for maintaining basal signaling. Density functional theory (DFT) calculations revealed that Cu centers act as the primary catalytic sites, while Zn ions stabilize the framework and assist substrate binding, thereby promoting phosphodiester bond cleavage through an adsorption–electron-transfer–hydrolysis pathway. These findings suggest that Cu/Zn-ZIF provides a robust platform for moderating pathological cAMP elevation and offer a mechanistic basis for the rational design of metal–organic framework catalysts for bio-relevant nucleotide regulation and broader biomedical applications.","PeriodicalId":7788,"journal":{"name":"Analytical Letters","volume":"1 1","pages":"1-17"},"PeriodicalIF":0.0,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147915556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical Detection of Hydroquinone (HQ) and Catechol (CC) in Environmental Samples Using a Pretreated Glassy Carbon Electrode (p-GCE) Modified with a Vertically-Ordered Mesoporous Silica Film (VMSF) 垂直有序介孔硅膜修饰的预处理玻碳电极（p-GCE）电化学检测环境样品中的对苯二酚（HQ）和儿茶酚（CC）

4区化学

Analytical Letters Pub Date : 2025-12-15 DOI: 10.1080/00032719.2025.2598341

Qinqin Sun, Jing Wang, Zhengzheng Liu, Lujie Wang, Jun Zhang, Binxing Liu, Liqiong Huang, Fei Yan, Jinsong Liu

{"title":"Electrochemical Detection of Hydroquinone (HQ) and Catechol (CC) in Environmental Samples Using a Pretreated Glassy Carbon Electrode (p-GCE) Modified with a Vertically-Ordered Mesoporous Silica Film (VMSF)","authors":"Qinqin Sun, Jing Wang, Zhengzheng Liu, Lujie Wang, Jun Zhang, Binxing Liu, Liqiong Huang, Fei Yan, Jinsong Liu","doi":"10.1080/00032719.2025.2598341","DOIUrl":"https://doi.org/10.1080/00032719.2025.2598341","url":null,"abstract":"Convenient and sensitive detection of hydroquinone (HQ) and catechol (CC) in environmental samples holds great importance for monitoring. Herein, a vertically-ordered mesoporous silica film (VMSF) supported on the electrochemically pretreated glassy carbon electrode (p-GCE) is prepared by a simple and environmentally friendly electrochemical method and employed to construct a sensitive and anti-fouling sensing interface for quantitative detection of HQ and CC. In virtue of a simple electrochemical polarization, the p-GCE shows enhanced electroactive area and promoted electrochemical activity, as well as oxygen-containing groups, rendering a sensitive electrode substrate with improved analytical performance and the functional interface for stable growth of VMSF. The obtained VMSF/p-GCE has superior electroanalytical capacity toward HQ and CC due to the synergistic effect of hydrogen bonding of VMSF and the sensitive p-GCE electrode substrate. The developed VMSF/p-GCE sensor enables the determination of HQ and CC with a wide linear range (1 ∼ 120 μM), high sensitivity (0.368 μA/μM for HQ and 0.404 μA/μM) and low limit of detection (268 nM for HQ and 80 nM for CC). Furthermore, the capacity of the prepared VMSF/p-GCE in the analysis of HQ and CC in environmental water samples and soil leaching solutions has been studied, showing acceptable results and therefore offering a simple and low-cost way for environmental analysis.","PeriodicalId":7788,"journal":{"name":"Analytical Letters","volume":"1 1","pages":"1-18"},"PeriodicalIF":0.0,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147921982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-Free Evaluation of Human Serum Albumin Using Ordered Porous Layer Optical Interferometry 使用有序多孔层光学干涉法对人血清白蛋白进行无标记评价

4区化学

Analytical Letters Pub Date : 2025-12-03 DOI: 10.1080/00032719.2025.2595153

Tianze Wang, Lu Wang, Yu Zhang, Li Liu, Bo Zhang, Weiping Qian

{"title":"Label-Free Evaluation of Human Serum Albumin Using Ordered Porous Layer Optical Interferometry","authors":"Tianze Wang, Lu Wang, Yu Zhang, Li Liu, Bo Zhang, Weiping Qian","doi":"10.1080/00032719.2025.2595153","DOIUrl":"https://doi.org/10.1080/00032719.2025.2595153","url":null,"abstract":"The determination of human serum albumin (HSA) is of great significance for the noninvasive diagnosis and treatment of modern diseases. Label-free immunoassay has been regarded as one of the most prominent techniques for HSA detection because of its reliability and sensitivity. In this work, an optical label-free biosensing method based on ordered porous polystyrene inverse opal (PS-IO) substrate was developed. Based on the methodology of inhibitory immunoassay, human serum albumin (HSA) was immobilized on the PS substrate with sufficient density, enabling one-step detection, while the interconnected inverse opal substrate enhances the mass transfer efficiency of the sensing unit. HSA level can be determined without either the label of protein molecules or sensor signal amplification, which simplifies the operation procedure and improves the detection efficiency. The linear range of the developed sensing system is 0.05–50 μg/mL, with a limit of quantitation of 50 ng/mL. At the same time, the sensor has strong selectivity and specificity, resists environmental molecular interference, and maintains stable sensing performance during long-term storage and recycling. This study provides valuable insights for the development of new clinical measurement devices.","PeriodicalId":7788,"journal":{"name":"Analytical Letters","volume":"1 1","pages":"1-19"},"PeriodicalIF":0.0,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silicon Quantum Dot-Gold Nanoparticle Hybrid Probe for Thrombin Detection via Inner Filter Effect 硅量子点-金纳米粒子混合探针内滤检测凝血酶

4区化学

Analytical Letters Pub Date : 2025-11-25 DOI: 10.1080/00032719.2025.2593582

Kun Zhang, Jinfeng Jia

引用次数: 0

Novel Immunosensor via Dual-Species Antibody Synergy: Guinea Pig Immunoglobulin G (IgG)/Chicken Immunoglobulin Y (IgY)-Mediated Specific Determination of Staphylococcus aureus 基于双种抗体协同作用的新型免疫传感器：豚鼠免疫球蛋白G (IgG)/鸡免疫球蛋白Y （IgY）介导的金黄色葡萄球菌特异性检测

4区化学

Analytical Letters Pub Date : 2025-11-20 DOI: 10.1080/00032719.2025.2587839

Yongsheng Chang, Xiane Guo, Xun Ge, Xing Yu, Shujun Ma, Canyu Liu, Yunwei Lou, Tingmin Chang, Yun Zhang

{"title":"Novel Immunosensor via Dual-Species Antibody Synergy: Guinea Pig Immunoglobulin G (IgG)/Chicken Immunoglobulin Y (IgY)-Mediated Specific Determination of Staphylococcus aureus","authors":"Yongsheng Chang, Xiane Guo, Xun Ge, Xing Yu, Shujun Ma, Canyu Liu, Yunwei Lou, Tingmin Chang, Yun Zhang","doi":"10.1080/00032719.2025.2587839","DOIUrl":"https://doi.org/10.1080/00032719.2025.2587839","url":null,"abstract":"Traditional immunoassays for Staphylococcus aureus (S. aureus) suffer from significant limitations, particularly owing to cross-reactivity with protein G-producing Streptococcus species. To address this issue, here is reported an innovative colorimetric strategy that depended on the unique binding characteristics of guinea pig immunoglobulin G (IgG) toward staphylococcal protein A (SPA), a highly expressed epitope on S. aureus. Unlike conventional monoclonal or polyclonal antibodies, guinea pig IgG exhibits high specificity for SPA, while demonstrating negligible affinity for protein G. The method also integrated horseradish peroxidase (HRP)-conjugated chicken anti-protein A immunoglobulin Y (IgY) (HRP–IgY) in a sandwich immunoassay format, enabling the catalytic oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) and hydrogen peroxide. The signal intensity correlated linearly with S. aureus concentration over a dynamic range of 7.8 × 102 CFU/mL–1.0 × 105 CFU/mL, achieving a detection limit of 6.9 × 102 CFU/mL. Notably, the assay demonstrated exceptional specificity, with no cross-reactivity with other common foodborne pathogens. Compared with mainstream methods, this assay completed the entire analytical process within 90 min-shorter than polymerase chain reaction (PCR) and traditional enzyme-linked immunosorbent assay (ELISA). It eliminated the need for complex DNA extraction or strict anti-cross-contamination controls and maintained stable performance in real samples. This advantage outperforms PCR (often susceptible to matrix inhibitors) and ELISA (typically exhibits reduced sensitivity in complex matrices). By offering significant improvements over conventional techniques, this strategy holds great promise for enhancing the efficiency and reliability of S. aureus detection in practical applications.","PeriodicalId":7788,"journal":{"name":"Analytical Letters","volume":"1 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147881550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Ultra-Sensitive Electrochemical Sensor Based Upon Nanoconfined Bimetallic Fe@PCN-222(Mn) for the Determination of Mercury in Natural Waters 基于纳米双金属Fe@PCN-222（Mn）的超灵敏电化学传感器测定自然水体中的汞

4区化学

Analytical Letters Pub Date : 2025-11-10 DOI: 10.1080/00032719.2025.2584265

Tingting Hu, Yue-Cheng Kang, Xinxin Lv, Yao Liu, Xing Chen

{"title":"Ultra-Sensitive Electrochemical Sensor Based Upon Nanoconfined Bimetallic Fe@PCN-222(Mn) for the Determination of Mercury in Natural Waters","authors":"Tingting Hu, Yue-Cheng Kang, Xinxin Lv, Yao Liu, Xing Chen","doi":"10.1080/00032719.2025.2584265","DOIUrl":"https://doi.org/10.1080/00032719.2025.2584265","url":null,"abstract":"Mercury ions (Hg2+) is a global pollutant that pose significant threats to human health and the environment. In this work, an electrochemical sensor was developed for the rapid determination of Hg2+ in groundwater. A novel bimetallic active center (Fe@PCN-222(Mn), hereinafter referred to as F@PM) was created by doping Fe0 uniformly into the channels of a manganese-based metal-organic framework (PCN-222(Mn)). The fabricated nanocomposite was characterized using x-ray diffraction (XRD), Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), Brunauer–Emmett–Teller (BET) and X-ray photoelectron spectroscopy (XPS) to characterize its morphology and structure. The sensing of F@PM modified glassy carbon electrode (F@PM/GCE) for Hg2+ was investigated using square wave anodic stripping voltammetry (SWASV). F@PM/GCE exhibited high sensitivity (24.56 μA/μM) and an exceptionally limit of detection (LOD) of 3.31 nM for Hg2+. The results are consistent with the linear range (100 ∼ 1000 nM). The transition metals Fe and Mn significantly augmented the electron transfer kinetics within the system, leveraging their distinct electronegativity values to optimize the redox processes, thereby elevating the overall performance of the electrochemical reaction. Importantly, the sensor showed excellent selectivity, with no interferences from common cations such as Cu2+, Pb2+, and Cd2+. Moreover, the F@PMmodified GCE demonstrated outstanding accuracy, reliable precision, and reproducibility. The sensors used in the natural water samples maintained their high accuracy and desirable recovery rates.","PeriodicalId":7788,"journal":{"name":"Analytical Letters","volume":"1 1","pages":"1-19"},"PeriodicalIF":0.0,"publicationDate":"2025-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0