Journal of molecular and applied genetics最新文献_第10页

Construction and characterization of the host component of the Bacillus subtilis HV2 cloning system. 枯草芽孢杆菌HV2克隆系统宿主成分的构建与表征。

Journal of molecular and applied genetics Pub Date : 1981-01-01

W F Burke, H T Le

引用次数: 0

Sets of immunoglobulin V kappa genes homologous to ten cloned V kappa sequences: implications for the number of germline V kappa genes. 与十个克隆的V kappa序列同源的免疫球蛋白V kappa基因组:对种系V kappa基因数量的影响。

Journal of molecular and applied genetics Pub Date : 1981-01-01

S Cory, B M Tyler, J M Adams

{"title":"Sets of immunoglobulin V kappa genes homologous to ten cloned V kappa sequences: implications for the number of germline V kappa genes.","authors":"S Cory, B M Tyler, J M Adams","doi":"","DOIUrl":"","url":null,"abstract":"To count V kappa genes homologous to particular V kappa nucleotide sequences, thereby permitting estimates for the total V kappa repertoire, we hybridized 10 cloned V kappa cDNA sequences to restriction fragments of mouse embryo DNA (Southern blots). Particular probes labeled up to 17 fragments, of which up to 8 were strongly labeled. This indicates that the germline contains sets of related V kappa genes. Only 4 nonoverlapping gene sets of 16-22 genes each were found. Assuming that the probes used are a random sample of the V kappa pool, the pattern repetitions suggest that the germline contains a total of about 5 distinct V kappa gene sets and about 90 V kappa genes. Correlating sets of strongly labeled genes with V kappa groups having similar N-terminal sequences led to an estimate of about 300 germline V kappa genes, while extrapolation from the number of genes in the VK-21 group gave an estimate of 90-140 genes. Since the three independent estimates fell between 90 and 320 genes, the germline V kappa repertoire, at least as expressed in myelomas, probably lies in that range. The V kappa fragment patterns given by three probes did not differ detectably between BALB/c, NZB, and A/J DNA but all three patterns differed in AKR DNA, indicating that there is limited polymorphism within the mouse V kappa locus. Somatic mutation must expand the number of expressed V kappa sequences, since the 8-12 VK-21 genes estimated for BALB/c and for NZB is significantly less than the 22 known NZB VK-21 polypeptides.","PeriodicalId":77864,"journal":{"name":"Journal of molecular and applied genetics","volume":"1 2","pages":"103-16"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17349480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An immunoglobulin VH pseudogene. 免疫球蛋白VH假基因。

Journal of molecular and applied genetics Pub Date : 1981-01-01

H Huang, S Crews, L Hood

引用次数: 0

Co-expression and amplification of dihydrofolate reductase cDNA and the Escherichia coli XGPRT gene in Chinese hamster ovary cells. 二氢叶酸还原酶cDNA与大肠杆菌XGPRT基因在中国仓鼠卵巢细胞中的共表达与扩增。

Journal of molecular and applied genetics Pub Date : 1981-01-01

G Ringold, B Dieckmann, F Lee

引用次数: 0

The gene encoding the common alpha subunit of the four human glycoprotein hormones. 编码四种人类糖蛋白激素的共同α亚基的基因。

Journal of molecular and applied genetics Pub Date : 1981-01-01

J C Fiddes, H M Goodman

{"title":"The gene encoding the common alpha subunit of the four human glycoprotein hormones.","authors":"J C Fiddes, H M Goodman","doi":"","DOIUrl":"","url":null,"abstract":"The gene encoding the common alpha subunit of the four human glycoprotein hormones, chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH), has been cloned in a bacteriophage lambda vector. Restriction endonuclease digestion of total human DNA suggests that the common alpha subunit is coded for by a single gene. Three distinct polymorphic hybridization patterns have been observed for this gene in the human population. The cloned gene encompasses a total of 9.4 kilobases (kb) and contains three intervening sequences whose locations have been established by restriction enzyme mapping and by DNA sequencing. One of the intervening sequences is located in the 5' untranslated region, generating a leader sequence that is separated from the rest of the gene by 6.4 kb. The other two intervening sequences are 1.7 and 0.4 kb long and are located within codon number 6, and between codons 67 and 68, respectively. The location of the 5' end of the mature transcript has been established by priming placental mRNA with a restriction fragment obtained from the cloned cDNA. A transcript of similar size for the alpha subunit gene has been detected in both the pituitary, where the gene is expressed for the synthesis of LH, FSH, and TSH, and the placenta, where the gene is expressed for the synthesis of CG. When parts of the 5' untranslated nucleotide sequences of the alpha subunit and the human growth hormone genes are compared a highly homologous region is observed. These otherwise unrelated genes share the common feature that they encode a secreted pituitary polypeptide hormone.","PeriodicalId":77864,"journal":{"name":"Journal of molecular and applied genetics","volume":"1 1","pages":"3-18"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17349478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of overproducers of the bacteriophage 434 repressor and cro proteins. 噬菌体434抑制因子和交叉蛋白的过量生产者的构建。

Journal of molecular and applied genetics Pub Date : 1981-01-01

G Lauer, R Pastrana, J Sherley, M Ptashne

引用次数: 0

The structures of the spliced mRNAs encoding polyoma virus early region proteins. 编码多瘤病毒早期区蛋白的剪接mrna的结构。

Journal of molecular and applied genetics Pub Date : 1981-01-01

R Treisman, A Cowie, J Favaloro, P Jat, R Kamen

引用次数: 0

Nonfunctional thyroglobulin messenger RNA in goats with hereditary congenital goiter. 遗传性先天性甲状腺肿山羊无功能甲状腺球蛋白信使RNA。

Journal of molecular and applied genetics Pub Date : 1981-01-01

J J de Vijlder, G J van Ommen, W F van Voorthuizen, C A Koch, A C Arnberg, G Vassart, C Dinsart, R A Flavell

引用次数: 0

Biosynthesis of subunits of the soybean 7S storage protein. 大豆 7S 储存蛋白亚基的生物合成。

Journal of molecular and applied genetics Pub Date : 1981-01-01

R N Beachy, N P Jarvis, K A Barton

{"title":"Biosynthesis of subunits of the soybean 7S storage protein.","authors":"R N Beachy, N P Jarvis, K A Barton","doi":"","DOIUrl":"","url":null,"abstract":"Biosynthesis of the alpha', alpha, and beta subunits of the soybean 7S storage protein (conglycinin) was studied by in vivo and in vitro experiments. One hour in vivo labeling produced polypeptides of 72 kilodaltons (kD) and 78 kD which, in a subsequent 1-h chase period, gave rise to a heterodisperse band of polypeptides of 76-83 kD, the apparent molecular weights of mature alpha and alpha', respectively. After a 3-h chase period only mature alpha and alpha' were labeled. The pre-alpha' (80 kD) and pre-alpha (78 kD) polypeptides produced by in vitro translation of total seed poly(A)+ RNA did not coelectrophorese with polypeptides labeled in vivo. The mature beta-subunit (53 kD), produced in vivo during the 1-h chase period, apparently was translated as a 50 kD polypeptide in vitro. The data suggest that, in vivo, primary translation products undergo both cotranslational and posttranslational modifications during the formation of mature subunits. cDNA clones complementary to soybean seed poly(A)+ RNA were prepared and subsequently identified by hybrid-release and immunoprecipitation experiments. Clone pGmc 236 (550 base pairs) (bp) was shown to contain sequences complementary to mRNAs for the alpha', alpha, and beta subunits on the basis of hybrid-release experiments, corroborating tryptic fingerprints that demonstrate that these subunits are closely related to each other. Although selected by a single cDNA clone, the mRNAs are not identical because the alpha' mRNA was eluted from the filter-bound cDNAs at a lower temperature than were the alpha beta mRNAs, pGmc 236 hybridized on Northern blots to mRNAs of approximately 2500 nucleotides, sufficient size to code for the alpha' or alpha subunit of the 7S storage protein.","PeriodicalId":77864,"journal":{"name":"Journal of molecular and applied genetics","volume":"1 1","pages":"19-27"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18217783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-step cloning of the Escherichia coli regulatory gene ompB, employing phage Mu. 利用噬菌体Mu两步克隆大肠杆菌调控基因ompB。

Journal of molecular and applied genetics Pub Date : 1981-01-01

E T Wurtzel, N R Movva, F L Ross, M Inouye

{"title":"Two-step cloning of the Escherichia coli regulatory gene ompB, employing phage Mu.","authors":"E T Wurtzel, N R Movva, F L Ross, M Inouye","doi":"","DOIUrl":"","url":null,"abstract":"It is difficult to clone directly some regulatory or structural genes on the basis of their functions, because of their obscure properties or the leakiness of their mutants. To overcome this problem, a two-step cloning method with use of phage Mu was developed and applied to cloning of the ompB gene, an obscure regulatory gene for major outer membrane proteins of Escherichia coli. The ompB gene was first inactivated by phage Mu insertion, and the approximately 25-kilobase (kb) EcoRI fragment, which hybridized with phage Mu DNA, was cloned into a plasmid vector, pBR322. This DNA fragment was considered to contain not only a portion of phage Mu DNA but also a portion of the ompB gene DNA. With this DNA as a probe, the wild-type ompB+ strain was found to contain a 12.7-kb EcoRI fragment which hybridized with the probe. In the second step, this 12.7-kb EcoRI fragment was cloned into a lambda phage vector, lambda 569. Lysogenization of an ompB mutant with this phage suppressed OmpB- phenotypes, indicating that the 12.7-kb EcoRI fragment carried the ompB gene. The same 12.7-kb DNA fragment, as well as a 3.8-kb EcoRI-BamHI subfragment, was cloned into pBR322. Both plasmid clones were able to suppress the OmpB- phenotypes upon transformation of an ompB mutant.","PeriodicalId":77864,"journal":{"name":"Journal of molecular and applied genetics","volume":"1 1","pages":"61-9"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17277674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0