Analytical Chemistry Insights最新文献_第2页

Analytical Method Validation of High-Performance Liquid Chromatography and Stability-Indicating Study of Medroxyprogesterone Acetate Intravaginal Sponges. 醋酸甲羟孕酮阴道内海绵的高效液相色谱分析方法验证及稳定性研究。

Analytical Chemistry Insights Pub Date : 2017-02-20 eCollection Date: 2017-01-01 DOI: 10.1177/1177390117690152

Nidal Batrawi, Shorouq Wahdan, Murad Abualhasan

{"title":"Analytical Method Validation of High-Performance Liquid Chromatography and Stability-Indicating Study of Medroxyprogesterone Acetate Intravaginal Sponges.","authors":"Nidal Batrawi, Shorouq Wahdan, Murad Abualhasan","doi":"10.1177/1177390117690152","DOIUrl":"https://doi.org/10.1177/1177390117690152","url":null,"abstract":"Medroxyprogesterone acetate is widely used in veterinary medicine as intravaginal dosage for the synchronization of breeding cycle in ewes and goats. The main goal of this study was to develop reverse-phase high-performance liquid chromatography method for the quantification of medroxyprogesterone acetate in veterinary vaginal sponges. A single high-performance liquid chromatography/UV isocratic run was used for the analytical assay of the active ingredient medroxyprogesterone. The chromatographic system consisted of a reverse-phase C18 column as the stationary phase and a mixture of 60% acetonitrile and 40% potassium dihydrogen phosphate buffer as the mobile phase; the pH was adjusted to 5.6. The method was validated according to the International Council for Harmonisation (ICH) guidelines. Forced degradation studies were also performed to evaluate the stability-indicating properties and specificity of the method. Medroxyprogesterone was eluted at 5.9 minutes. The linearity of the method was confirmed in the range of 0.0576 to 0.1134 mg/mL (R2 > 0.999). The limit of quantification was shown to be 3.9 µg/mL. Precision and accuracy ranges were found to be %RSD <0.2 and 98% to 102%, respectively. Medroxyprogesterone capacity factor value of 2.1, tailing factor value of 1.03, and resolution value of 3.9 were obtained in accordance with ICH guidelines. Based on the obtained results, a rapid, precise, accurate, sensitive, and cost-effective analysis procedure was proposed for quantitative determination of medroxyprogesterone in vaginal sponges. This analytical method is the only available method to analyse medroxyprogesterone in veterinary intravaginal dosage form.","PeriodicalId":7781,"journal":{"name":"Analytical Chemistry Insights","volume":"12 ","pages":"1177390117690152"},"PeriodicalIF":0.0,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1177390117690152","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34966271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Insight into Biochemical Characterization of Plant Sesquiterpene Synthases 植物倍半萜合成酶的生化特性研究

Analytical Chemistry Insights Pub Date : 2016-09-29 DOI: 10.4137/ACI.S40292

Tom Manczak, H. T. Simonsen

引用次数: 1

Development of Hollow-Fiber Liquid-Phase Microextraction Method for Determination of Urinary trans,trans-Muconic Acid as a Biomarker of Benzene Exposure 中空纤维液相微萃取法测定尿中苯暴露生物标志物反式、反式粘膜酸的建立

Analytical Chemistry Insights Pub Date : 2016-09-11 DOI: 10.4137/ACI.S40177

F. Ghamari, A. Bahrami, Y. Yamini, F. G. Shahna, A. Moghimbeigi

引用次数: 24

Stability-Indicating High-Performance Liquid Chromatography Assay for the Determination of Sulthiame in Pharmaceutical Dosage Forms 稳定性指示高效液相色谱法测定药物剂型中磺胺的含量

Analytical Chemistry Insights Pub Date : 2016-09-06 DOI: 10.4137/ACI.S38656

A. Haidar, S. Kabiche, E. Majoul, I. Balde, J. Fontan, S. Cisternino, J. Schlatter

引用次数: 1

Extraction of Honey Polyphenols: Method Development and Evidence of Cis Isomerization. 蜂蜜多酚的提取:方法发展及顺式异构化证据。

Analytical Chemistry Insights Pub Date : 2016-08-10 eCollection Date: 2016-01-01 DOI: 10.4137/ACI.S39739

Thibaut Istasse, Nicolas Jacquet, Thomas Berchem, Eric Haubruge, Bach Kim Nguyen, Aurore Richel

{"title":"Extraction of Honey Polyphenols: Method Development and Evidence of Cis Isomerization.","authors":"Thibaut Istasse, Nicolas Jacquet, Thomas Berchem, Eric Haubruge, Bach Kim Nguyen, Aurore Richel","doi":"10.4137/ACI.S39739","DOIUrl":"https://doi.org/10.4137/ACI.S39739","url":null,"abstract":"Honey polyphenols have been studied with the objective of relating honeys to their floral sources. Initially synthesized by plant, these polyphenols can be found in the plant's nectar, which are collected by bees, which convert the nectar into honey. Consequently, polyphenols constitute minor components of honey. The development of a solid-phase extraction method for honey polyphenols is presented in this study. The technique employs Amberlite XAD-2 adsorbent and was tested on monofloral honeys from six different plants: acacia, chestnut, eucalyptus, thyme, sunflower, and wild carrot. Analyses were performed using high-performance liquid chromatography coupled with UV detection and mass spectrometry. Several phenolic acids and flavonoids were identified: caffeic and p-coumaric acids, quercetin, kaempferol, naringenin, chrysin, and pinocembrin. Generally, the quantity of a given polyphenol in the honey was around 0.2 mg/100 g of honey, except for chestnut honey, which contained around 3.0 mg of p-coumaric acid/100 g of honey. Analyses highlighted significant formation of cis isomers for phenolic acids during the extraction despite protection from light. ","PeriodicalId":7781,"journal":{"name":"Analytical Chemistry Insights","volume":"11 ","pages":"49-57"},"PeriodicalIF":0.0,"publicationDate":"2016-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/ACI.S39739","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34324497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

On the Conflicting Estimations of Pigment Site Energies in Photosynthetic Complexes: A Case Study of the CP47 Complex. 光合配合物中色素位能的冲突估计:以CP47配合物为例。

Analytical Chemistry Insights Pub Date : 2016-06-02 eCollection Date: 2016-01-01 DOI: 10.4137/ACI.S32151

Tonu Reinot, Jinhai Chen, Adam Kell, Mahboobe Jassas, Kevin C Robben, Valter Zazubovich, Ryszard Jankowiak

{"title":"On the Conflicting Estimations of Pigment Site Energies in Photosynthetic Complexes: A Case Study of the CP47 Complex.","authors":"Tonu Reinot, Jinhai Chen, Adam Kell, Mahboobe Jassas, Kevin C Robben, Valter Zazubovich, Ryszard Jankowiak","doi":"10.4137/ACI.S32151","DOIUrl":"https://doi.org/10.4137/ACI.S32151","url":null,"abstract":"We focus on problems with elucidation of site energies [Formula: see text] for photosynthetic complexes (PSCs) in order to raise some genuine concern regarding the conflicting estimations propagating in the literature. As an example, we provide a stern assessment of the site energies extracted from fits to optical spectra of the widely studied CP47 antenna complex of photosystem II from spinach, though many general comments apply to other PSCs as well. Correct values of [Formula: see text] for chlorophyll (Chl) a in CP47 are essential for understanding its excitonic structure, population dynamics, and excitation energy pathway(s). To demonstrate this, we present a case study where simultaneous fits of multiple spectra (absorption, emission, circular dichroism, and nonresonant hole-burned spectra) show that several sets of parameters can fit the spectra very well. Importantly, we show that variable emission maxima (690-695 nm) and sample-dependent bleaching in nonresonant hole-burning spectra reported in literature could be explained, assuming that many previously studied CP47 samples were a mixture of intact and destabilized proteins. It appears that the destabilized subpopulation of CP47 complexes could feature a weakened hydrogen bond between the 13(1)-keto group of Chl29 and the PsbH protein subunit, though other possibilities cannot be entirely excluded, as discussed in this work. Possible implications of our findings are briefly discussed. ","PeriodicalId":7781,"journal":{"name":"Analytical Chemistry Insights","volume":"11 ","pages":"35-48"},"PeriodicalIF":0.0,"publicationDate":"2016-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/ACI.S32151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34452744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Removal of 4-Nitrophenol from Water Using Ag-N-P-Tridoped TiO2 by Photocatalytic Oxidation Technique. ag - n - p -三掺杂TiO2光催化氧化法去除水中4-硝基苯酚

Analytical Chemistry Insights Pub Date : 2016-04-04 eCollection Date: 2016-01-01 DOI: 10.4137/ACI.S31508

Temesgen Achamo, O P Yadav

{"title":"Removal of 4-Nitrophenol from Water Using Ag-N-P-Tridoped TiO2 by Photocatalytic Oxidation Technique.","authors":"Temesgen Achamo, O P Yadav","doi":"10.4137/ACI.S31508","DOIUrl":"https://doi.org/10.4137/ACI.S31508","url":null,"abstract":"Photocatalytic oxidation using semiconductor nanoparticles is an efficient, eco-friendly, and cost-effective process for the removal of organic pollutants, such as dyes, pesticides, phenols, and their derivatives in water. In the present study, nanosize Ag-N-P-tridoped titanium(IV) oxide (TiO2) was prepared by using sol-gel-synthesized Ag-doped TiO2 and soybean (Glycine max) or chickpea (Cicer arietinum) seeds as nonmetallic bioprecursors. As-synthesized photocatalysts were characterized using X-ray diffraction, Fourier transform infrared, and ultra violet (UV)-visible spectroscopic techniques. Average crystallite size of the studied photocatalysts was within 39-46 nm. Whereas doped Ag in TiO2 minimized the photogenerated electron-hole recombination, doped N and P extended its photoabsorption edge to visible region. Tridoping of Ag, N, and P in TiO2 exhibited synergetic effect toward enhancing its photocatalytic degradation of 4-nitrophenol (4-NP), separately, under UV and visible irradiations. At three hours, degradations of 4-NP over Ag-N-P-tridoped TiO2 under UV and visible radiations were 73.8 and 98.1%, respectively. ","PeriodicalId":7781,"journal":{"name":"Analytical Chemistry Insights","volume":"11 ","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"2016-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/ACI.S31508","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34404738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Development and Validation of Stability-Indicating RP-HPLC Method for Simultaneous Determination of Metformin HCl and Glimepiride in Fixed-Dose Combination 稳定指示反相高效液相色谱法同时测定盐酸二甲双胍和格列美脲的含量

Analytical Chemistry Insights Pub Date : 2016-03-13 DOI: 10.4137/ACI.S38137

Pradnya N. Vaingankar, P. Amin

{"title":"Development and Validation of Stability-Indicating RP-HPLC Method for Simultaneous Determination of Metformin HCl and Glimepiride in Fixed-Dose Combination","authors":"Pradnya N. Vaingankar, P. Amin","doi":"10.4137/ACI.S38137","DOIUrl":"https://doi.org/10.4137/ACI.S38137","url":null,"abstract":"A simple reversed-phase high-performance liquid chromatography method was developed and validated for simultaneous determination of Metformin hydrochloride (MET) and Glimepiride (GLM) in combination and estimation of their principal degradation products. The separation was achieved using JASCO Finepak SIL (250 mm × 4.6 mm i.d. 5 μm) at ambient temperature. The optimized mobile phase composed of an aqueous phase (20 mM phosphate buffer, adjusted to pH 3.0) and an organic phase (methanol:acetonitrile; 62.5:37.5) in the ratio of 80:20. The flow rate was 1 mL/minute, and the analytes were detected at 230 nm. The developed method was validated for accuracy, precision, specificity, linearity, and sensitivity. The chromatographic analysis time was approximately six minutes with the complete resolution of MET (Rt = 2.75 minutes) and GLM (Rt = 5.87 minutes). The method exhibited good linearity over the range of 5–30 μg/mL for MET and 1–10 μg/mL for GLM. The drugs in combination were subjected to various stress degradation studies as per the International Conference Harmonization (ICH) guidelines. Results obtained from the stress degradation studies revealed that the developed method is applicable for stability studies.","PeriodicalId":7781,"journal":{"name":"Analytical Chemistry Insights","volume":"107 1","pages":"13 - 20"},"PeriodicalIF":0.0,"publicationDate":"2016-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77441406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Electrochemical Behavior and Voltammetric Determination of a Manganese(II) Complex at a Carbon Paste Electrode 锰(II)配合物在碳糊电极上的电化学行为和伏安测定

Analytical Chemistry Insights Pub Date : 2016-01-18 DOI: 10.4137/ACI.S32150

Sophia Karastogianni, S. Girousi

引用次数: 7

Sample Preparation of Eggs From Laying Hens Using QuEChERS Dispersive Extraction for the Simultaneous Determination of Melamine and Cyromazine Residues by HPLC-DAD QuEChERS分散萃取法制备蛋鸡蛋样品，HPLC-DAD法同时测定三聚氰胺和氰胺残留

Analytical Chemistry Insights Pub Date : 2015-12-20 DOI: 10.4137/ACI.S31727

Niki Tsartsali, V. Samanidou

{"title":"Sample Preparation of Eggs From Laying Hens Using QuEChERS Dispersive Extraction for the Simultaneous Determination of Melamine and Cyromazine Residues by HPLC-DAD","authors":"Niki Tsartsali, V. Samanidou","doi":"10.4137/ACI.S31727","DOIUrl":"https://doi.org/10.4137/ACI.S31727","url":null,"abstract":"A quick, easy, cheap, effective, rugged, and safe (QuEChERS) dispersive extraction method is proposed herein for the isolation and cleanup of melamine and cyromazine from chicken egg yolk. Analytes are determined by high-performance liquid chromatography using photodiode array detector after separation on a LiChroCART® (250 × 4 mm)—LiChrospher® RP-8e, 5 μm analytical column using a mobile phase of 0.1% trifluoracetic acid and methanol (80:20 v/v) delivered isocratically at a flow rate of 1 mL/minute. Extraction of isolated compounds was achieved by methanol and acetonitrile mixture (1:1 v/v). Recovery rates ranged between 74.5% and 115.8%. The method was validated in terms of 657/2002/EC decision. The within-laboratory reproducibility, expressed as a relative standard deviation, was <11%. Decision limits (CCalfa) were 2.56 mg/kg for melamine and 0.22 mg/kg−1 for cyromazine, and the corresponding results for detection capability (CCbeta) were 2.8 mg/kg for melamine and 0.24 mg/kg for cyromazine. Ruggedness was estimated according to the Youden approach studying egg yolk mass, sorbent mass, centrifugation time, organic solvents volume, evaporation temperature, and vortex time.","PeriodicalId":7781,"journal":{"name":"Analytical Chemistry Insights","volume":"24 1","pages":"53 - 58"},"PeriodicalIF":0.0,"publicationDate":"2015-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72636809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6