Gene analysis techniques最新文献_第3页

Current methods for YAC clone characterization 目前YAC克隆鉴定方法

Gene analysis techniques Pub Date : 1990-09-01 DOI: 10.1016/0735-0651(90)90014-7

David L. Nelson

引用次数: 18

Modification and manipulation of mammalian DNA cloned as YACs 克隆为YACs的哺乳动物DNA的修饰和操作

Gene analysis techniques Pub Date : 1990-09-01 DOI: 10.1016/0735-0651(90)90015-8

Roger H. Reeves , William J. Pavan , Philip Hieter

引用次数: 11

Application of long synthetic oligonucleotides for gene analysis: Effect of probe length and stringency conditions on hybridization specificity 长合成寡核苷酸在基因分析中的应用:探针长度和严格条件对杂交特异性的影响

Gene analysis techniques Pub Date : 1990-06-01 DOI: 10.1016/0735-0651(90)90031-A

Yoshio Kajimura , Jacqueline Krull , Shoichi Miyakoshi , Keiichi Itakura , Hiroo Toyoda

引用次数: 4

Hybrid selection of mRNA with biotinylated DNA 生物素化DNA mRNA杂交选择

Gene analysis techniques Pub Date : 1990-06-01 DOI: 10.1016/0735-0651(90)90032-B

Jaemog Soh , Sidney Pestka

引用次数: 0

An improved strategy for generating a family of unidirectional deletions on large DNA fragments 在大的DNA片段上产生一个单向缺失家族的改进策略

Gene analysis techniques Pub Date : 1990-06-01 DOI: 10.1016/0735-0651(90)90033-C

Dominique Thomas, Yolande Surdin-Kerjan

引用次数: 15

Meetings and workshops calendar 会议及工作坊日程表

Gene analysis techniques Pub Date : 1990-06-01 DOI: 10.1016/0735-0651(90)90034-D

引用次数: 0

Affinity purification of specific DNA fragments using a lac repressor fusion protein 利用lac抑制融合蛋白亲和纯化特定DNA片段

Gene analysis techniques Pub Date : 1990-05-01 DOI: 10.1016/0735-0651(90)90040-M

Joakim Lundeberg, Johan Wahlberg, Mathias Uhlén

引用次数: 29

Structure-function studies of interferon-α based on random mutagenesis and expression in vitro 基于随机诱变和体外表达的干扰素α结构-功能研究

Gene analysis techniques Pub Date : 1990-05-01 DOI: 10.1016/0735-0651(90)90041-D

Martin J. Tymms , Beth McInnes , Per Ålin , Anthony W. Linnane , Brian F. Cheetham

{"title":"Structure-function studies of interferon-α based on random mutagenesis and expression in vitro","authors":"Martin J. Tymms , Beth McInnes , Per Ålin , Anthony W. Linnane , Brian F. Cheetham","doi":"10.1016/0735-0651(90)90041-D","DOIUrl":"https://doi.org/10.1016/0735-0651(90)90041-D","url":null,"abstract":"<div><p>An efficient procedure for random chemical mutagenesis was used to create analogs of human interferon (IFN)-α4. Unique restriction enzyme sites were introduced into the human IFN-α4 gene to enable cassetting of the gene for localized random mutagenesis. Single-stranded IFN-α4 DNA was treated with nitrous acid, followed by second-strand synthesis using reverse transcriptase. A 72 base pair cassette spanning the coding region for amino acid residues 120 to 136 (120–136 region) was isolated and cloned into a phagemid vector adjacent to a GC-rich sequence. A DNA segment comprising the IFN-α4 cassette sequence and the GC clamp was excised and electrophoresed on a denaturing gradient gel, which allowed the separation from unmutated DNA of DNA fragments with single base pair changes. DNA fragments with mobility different from that of the unmutated fragment were pooled and cloned into an expression vector. Using this procedure, mutations were found in the DNA of 48% of the clones analyzed. However, mutations at two “hot spots” accounted for 89% of these clones. Four of the IFN-α4 analogs with mutations in the 120–136 region were expressed <em>in vitro</em>. The antiproliferative activities on human Daudi cells of most of the analogs were less than 0.2% of the activity of unmodified IFN-α4, suggesting that the integrity of the carboxy terminus is important for the antiproliferative activity of human IFN-α4.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"7 3","pages":"Pages 53-63"},"PeriodicalIF":0.0,"publicationDate":"1990-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(90)90041-D","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71837139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Subtraction hybridization cDNA libraries from colon carcinoma and hepatic cancer 结肠癌和肝癌cDNA文库的减法杂交

Gene analysis techniques Pub Date : 1990-05-01 DOI: 10.1016/0735-0651(90)90042-E

Clifford W. Schweinfest , Kelly W. Henderson , Jian-Ren Gu , Stavros D. Kottaridis , Stavros Besbeas , Evi Panotopoulou , Takis S. Papas

引用次数: 71

Physical mapping of a temperature-sensitive mutation of human cytomegalovirus by marker rescue 人类巨细胞病毒温度敏感突变的标记拯救物理定位

Gene analysis techniques Pub Date : 1990-04-01 DOI: 10.1016/0735-0651(90)90009-5

Michel Dion , Jocelyn Yelle , Claude Hamelin

引用次数: 2