Annales de l'Institut Pasteur. Microbiology最新文献_第7页

Identification of Flavobacterium strains by gas liquid chromatographic analysis of volatile fatty acids produced in culture 培养过程中挥发性脂肪酸的气相色谱法鉴定黄杆菌菌株

Annales de l'Institut Pasteur. Microbiology Pub Date : 1988-07-01 DOI: 10.1016/0769-2609(88)90104-4

D. Rasoamananjara , J.C. Turlot , H. Monteil

引用次数: 3

Rapid serotyping of enteropathogenic Yersinia Enterocolitica strains by co-agglutination 用共凝集法快速分型肠致病性小肠结肠炎耶尔森菌

Annales de l'Institut Pasteur. Microbiology Pub Date : 1988-07-01 DOI: 10.1016/0769-2609(88)90108-1

L. Fernandez-Lago, M.S. Rodriguez-Nebreda, A. Chordi

引用次数: 3

Purified native haptens of Brucella abortus B19 and B. melitensis 16M reveal the lipopolysaccharide origin of the antigens 纯化的流产布鲁氏菌B19和melitensis B. 16M半抗原揭示了抗原的脂多糖来源

Annales de l'Institut Pasteur. Microbiology Pub Date : 1988-07-01 DOI: 10.1016/0769-2609(88)90105-6

M.S. Zygmunt , G. Dubray , D.R. Bundle , M.P. Perry

{"title":"Purified native haptens of Brucella abortus B19 and B. melitensis 16M reveal the lipopolysaccharide origin of the antigens","authors":"M.S. Zygmunt , G. Dubray , D.R. Bundle , M.P. Perry","doi":"10.1016/0769-2609(88)90105-6","DOIUrl":"10.1016/0769-2609(88)90105-6","url":null,"abstract":"<div>Purification of the Brucella polysaccharide referred to as native hapten (NH) and extracted from cells by the autoclaving procedure, was accomplished by ultrafiltration, followed by repetitive gel filtration using high-performance liquid chromatography on a ⪡TSK-G2000-SW⪢ column. The purified NH was analysed by SDS-PAGE, gas-liquid chromatography mass spectroscopy, and 13C and 1H NMR spectroscopy. NH from B. abortus B19 (NH-A) was shown to have a structure identical to that of A polysaccharide from B. abortus 1119-3, a linear homopolymer of α-1,2-linked-4,6-dideoxy-4-formamido-D-mannopyrannosyl residues.The structure of the NH from B. melitensis 16M (NH-M) was identified as a linear homopolysaccharide of the same sugar but composed of a pentasaccharide repeating unit in which four α1,2-linked-4,6-dideoxy-4-formamido-D-mannopyrannosyl residues are linked α-1,3 to the last monosaccharide of the sequence. This structure is similar to that determined for the Brucella M polysaccharide from B. melitensis 16M. The discovery in highly purified NH preparations of covalently bound monosaccharides characteristics of lipopolysaccharide inner core regions e.g., quinovosamine, mannose and 3-deoxy-D-manno-octulosonate (KDO), indicates that this polysaccharide is derived from lipopolysaccharides (LPS) by hydrolytic conditions fortuitously generated during the extraction protocol.The antigenically important polysaccharides of Brucelle are now established to be either A or M antigens. Polysaccharide B is a cyclic glucan with no structural or serological relationship were to A for M polysaccharides, its apparent activity in diagnostic tests of infected cattle resuls from O polysaccharide B.</div>","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 4","pages":"Pages 421-433"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90105-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13606066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

A novel approach to the evolution of microorganisms 研究微生物进化的新方法

Annales de l'Institut Pasteur. Microbiology Pub Date : 1988-07-01 DOI: 10.1016/0769-2609(88)90118-4

N. Peyriéras

引用次数: 0

On interspecies gene transfer: The case of the argF gene of Escherichia coli 种间基因转移:以大肠杆菌argF基因为例

Annales de l'Institut Pasteur. Microbiology Pub Date : 1988-07-01 DOI: 10.1016/0769-2609(88)90111-1

F. Van Vliet, A. Boyen, N. Glansdorff

引用次数: 22

L'antifongigramme des dermatophytes 美容玛丽

Annales de l'Institut Pasteur. Microbiology Pub Date : 1988-07-01 DOI: 10.1016/0769-2609(88)90110-X

R Steiman, F Seigle-Murandi, L Sage

引用次数: 0

Expression and maintenance of plasmid resistance in regenerating protoplasts of Bacillus subtilis 枯草芽孢杆菌再生原生质体质粒抗性的表达与维持

Annales de l'Institut Pasteur. Microbiology Pub Date : 1988-07-01 DOI: 10.1016/0769-2609(88)90103-2

C. Sanchez-Rivas

引用次数: 0

Le laboratoire de bactériologie médicale Equipment, techniques de base sécurité. 医学细菌学实验室设备，基本安全技术。

Annales de l'Institut Pasteur. Microbiology Pub Date : 1988-07-01 DOI: 10.1016/0769-2609(88)90112-3

J. Fleury

引用次数: 0

Adherence of Fusobacterium necrophorum biovar A and B strains to erythrocytes and tissue culture cells 坏死梭杆菌生物变种A株和B株对红细胞和组织培养细胞的粘附

Annales de l'Institut Pasteur. Microbiology Pub Date : 1988-07-01 DOI: 10.1016/0769-2609(88)90107-X

T. Shinjo, S. Miyazato , H. Kiyoyama

引用次数: 9

Immunological comparison of constitutive β-lactamases of gram-negative bacteria by neutralization in zymogram gels: Properties of anti-TEM-1 and anti-TEM-2 sera 酶谱凝胶中和革兰氏阴性菌组成型β-内酰胺酶的免疫学比较:抗tem -1和抗tem -2血清的特性

Annales de l'Institut Pasteur. Microbiology Pub Date : 1988-07-01 DOI: 10.1016/0769-2609(88)90106-8

G Paul , M Barthélémy , A Philippon , J Peduzzi , L Gilly , R Labia , P Névot

{"title":"Immunological comparison of constitutive β-lactamases of gram-negative bacteria by neutralization in zymogram gels: Properties of anti-TEM-1 and anti-TEM-2 sera","authors":"G Paul , M Barthélémy , A Philippon , J Peduzzi , L Gilly , R Labia , P Névot","doi":"10.1016/0769-2609(88)90106-8","DOIUrl":"10.1016/0769-2609(88)90106-8","url":null,"abstract":"<div>The zymogram technique was applied to a β-lactamase neutralization assay with anti-TEM-1 and anti-TEM-2 sera. Both were shown to contain neutralizing antibodies directed towards various β-lactamases of Gram-negative bacteria. The quantitative neutralization allowed classification into five groups of the 28 β-lactamases used as standards and 61 from clinical isolates. In the first were enzymes such as TEM-1 and TEM-2 including TLE-1, SHV-1, SHV-2, penicillinases of Klebsiella pneumoniae and CTX-1. Partial neutralization distinguished two groups containing the CARB group of enzymes, which are different from PSE-2 and PSE-3, and the MAL penicillinases of Levinea malonatica, which are different from L. amalonatica enzymes. Broad spectrum β-lactamases of K. oxytoca constituted a unique group of partially neutralized enzymes. Among the β-lactamases not neutralized by either serum were the plasmid-mediated OXA-enzymes, various species-specific β-lactamases and cephalosporinases.The antigenic similarities of the enzymes appeared to correlate with the extent of similarities of their catalytic properties, namely those of penicillinases. Such comparisons between the β-lactamase groups provide an indirect approach to the physiological and structural analysis of established and recently evolved β-lactamases.</div>","PeriodicalId":77666,"journal":{"name":"Annales de l'Institut Pasteur. Microbiology","volume":"139 4","pages":"Pages 435-451"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2609(88)90106-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78102995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12