Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement最新文献_第2页

Assay, purification and further characterization of 7S C1q-precipitins (C1q-p) in hypocomplementemic vasculitis urticaria syndrome and systemic lupus erythematosus. 7S c1q -沉淀素(C1q-p)在补乏性血管炎、荨麻疹综合征和系统性红斑狼疮中的检测、纯化和进一步表征。

Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement Pub Date : 1984-01-01

R J Marder, L A Potempa, J V Jones, D Toriumi, F R Schmid, H Gewurz

{"title":"Assay, purification and further characterization of 7S C1q-precipitins (C1q-p) in hypocomplementemic vasculitis urticaria syndrome and systemic lupus erythematosus.","authors":"R J Marder, L A Potempa, J V Jones, D Toriumi, F R Schmid, H Gewurz","doi":"","DOIUrl":"","url":null,"abstract":"C1q-precipitins (C1q-p) are comprised of 7S IgG with C1q-binding activity found in sera of patients with hypocomplementemic vasculitis urticaria syndrome ( HVUS ) and systemic lupus erythematosis (SLE). We have utilized C1q-coated polystyrene beads to selectively isolate C1q-p and to establish a sensitive and quantitative assay of C1q-p and immune complex activity. Purified C1q-p was comprised of polyclonal IgG which retained 7S sedimentation and solid phase C1q-binding activity at physiological ionic strength both in the presence and absence of normal human sera. No precipitation interaction was observed between C1q-p and fluid-phase C1q or C1 under the conditions tested. Purified C1q-p had no activity in the Raji cell immune complex activity. C1q-p activity also was observed in and purified from SLE serum; this activity was distinguishable from 7S immune complex activity detected by Raji cells which was also present in SLE serum. These studies indicate that C1q-p is a 7S IgG molecule found in HVUS as well as some SLE sera and has activity in C1q-binding but not in Raji cell-binding immune complex assays. These data also suggest that C1q-p is a monomeric, polyclonal IgG with preferential affinity for bound C1q. In addition to its potential role in immune complex disease, C1q-p may also provide an important tool for studying the interaction of immunoglobulin and C1q, and should contribute important information to understanding the pathobiology of immune complex disease.","PeriodicalId":77654,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement","volume":"284 ","pages":"25-34"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17435208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cancer epidemiology. Some methodological reflections. 癌症流行病学。一些方法上的思考。

Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement Pub Date : 1984-01-01

L Kreyberg

引用次数: 0

Separation of functionally or highly pure C2 from human plasma with Sepharose and a lectin of Euonymus europeus. 用Sepharose和Euonymus凝集素从人血浆中分离功能或高纯度的C2。

Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement Pub Date : 1984-01-01

D R Schultz, P I Arnold

引用次数: 0

Complement in the serum and venom of Brazilian snakes (Crotalidae). 巴西蛇(Crotalidae)血清和毒液中的补体。

Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement Pub Date : 1984-01-01

W Dias da Silva, V L Calich, T L Kipnis, F S Rosen, C A Alper

{"title":"Complement in the serum and venom of Brazilian snakes (Crotalidae).","authors":"W Dias da Silva, V L Calich, T L Kipnis, F S Rosen, C A Alper","doi":"","DOIUrl":"","url":null,"abstract":"Proteins antigenically related to CoF, the anticomplementary protein of the venom of the Indian hooded cobra (Naja naja), were found in a variety of elapid and viper venoms but not in the venom of Brazilian crotalids . In keeping with this finding was the weak ability of Brazilian snake venom to convert C3 in human serum. All snake serums tested, including Brazilian crotalids , contained a beta-globulin antigenically related to CoF. This serum protein in Brazilian snake serum had a number of characteristics of mammalian C3, including conversion on storage or on incubation of this serum with endotoxin, zymosan or mammalian antigen-antibody precipitates. The serum protein did not, however, convert on incubation with hydrazine. Brazilian crotalid serum did not, as did cobra serum, have the ability to inactivate CoF's ability to activate complement in normal human serum. The crotalid serum had hemolytic activity for rabbit antibody-sensitized and unsensitized sheep red blood cells that was active in the presence of Ca++ and Mg++ or Mg++ alone but greater with Ca++ present, suggesting the presence of both classical and alternative pathways of complement activation. This activity was maximal at 37 degrees C, but was destroyed or inactive after heating at 50 degrees C for 1 hr, incubation with hydrazine or by addition of EDTA. A marked reduction of hemolytic activity of Bothrops serum occurred after removal of the CoF-like protein. These findings suggest that Brazilian snake venom has little CoF-like material, but its serum contains a CoF-like protein with many characteristics of C3.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77654,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement","volume":"284 ","pages":"97-103"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17643542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lysis of sheep red cells in neat autologous serum as a source of antibody and complement. 羊红细胞在纯自体血清中的溶解，作为抗体和补体的来源。

Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement Pub Date : 1984-01-01

T Borsos, A Circolo

引用次数: 0

Inhibition of C9 polymerization within the SC5b-9 complex of complement by S-protein. S 蛋白抑制补体 SC5b-9 复合物内的 C9 聚合。

Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement Pub Date : 1984-01-01

E R Podack, K T Preissner, H J Müller-Eberhard

{"title":"Inhibition of C9 polymerization within the SC5b-9 complex of complement by S-protein.","authors":"E R Podack, K T Preissner, H J Müller-Eberhard","doi":"","DOIUrl":"","url":null,"abstract":"The effect of S-protein on the polymerization of C9 during assembly of the C5b-9 complex was examined. Utilizing SDS polyacrylamide gradient slab gel electrophoresis, tubular poly C9 was quantitated as SDS resistant protein of 1.1 to 1.3 X 10(6) molecular weight. Poly C9 formation occurred upon incubation of purified C5b-6, C7, C8 and C9 at molar ratios 1:1:1:12. Addition of purified S-protein to the protein mixture or to preassembled C5b-7 or C5b-8 blocked formation of poly C9 in a dose dependent fashion and gave rise to SC5b-9. SC5b-9 assembled from purified proteins or in zymosan-activated serum was visualized in the electron microscope as a wedge-shaped structure of 350 to 400 A length and 30 to 250 A width which lacked tubular poly C9 seen in images of the membrane attack complex (MAC). Using biotinyl-S-protein and colloidal gold particles coated with avidin, S-protein was located at the wide end of the wedge-like SC5b-9 complex. It is concluded that S-protein has a dual function in SC5b-9 assembly. It blocks the membrane site of C5b-7 and it inhibits C9 polymerization by SC5b-8. Accordingly, the main structural difference between SC5b-9 and the MAC is the lack of tubular poly C9.","PeriodicalId":77654,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement","volume":"284 ","pages":"89-96"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17643540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Freeze-thaw activation of the complement attack phase: II. Comparison of convertase generated C--56 with C--56 generated by freezing and thawing. 冻融激活补体攻击期:2。C—56转化酶与冻融法生成的C—56的比较。

Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement Pub Date : 1984-01-01

A Dessauer, U Rother, K Rother

引用次数: 0

The catabolism of C1(-)-inhibitor and the pathogenesis of hereditary angio-edema. C1(-)抑制剂的分解代谢与遗传性血管水肿的发病机制。

Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement Pub Date : 1984-01-01

P J Lachmann, F S Rosen

{"title":"The catabolism of C1(-)-inhibitor and the pathogenesis of hereditary angio-edema.","authors":"P J Lachmann, F S Rosen","doi":"","DOIUrl":"","url":null,"abstract":"A sufficient explanation for the observations that HAE is a dominantly transmitted disease and that the hemizygotes have levels of the normal protein of only in the region of 15%-20% of normal can be given by proposing that a substantial proportion of the catabolism of C1(-)-esterase inhibitor involves the prior formation of a complex with one of the enzymes with which the inhibitor reacts. This part of the catabolism will be largely independent of inhibitor concentration, i.e. of zero order, and for this reason occurs similarly in normals and in hemizygotes. Estimates of the extent of this zero order metabolism can be obtained from turnover data with normal and dysfunctional C1(-)-inhibitor and the results are consistent with the observed levels. In the form of the disease associated with the dysfunction protein the dysfunctional protein makes up more than 85% of the total protein found for the same reason. The extent of the enzyme inhibitor complex dependent catabolism (RO) can be determined in vivo by simultaneous turnovers of dysfunctional and normal inhibitor and gives a measure of the extent of activation of this group of enzymes. The value of this technique in clinical practice is described elsewhere.","PeriodicalId":77654,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement","volume":"284 ","pages":"35-9"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17643710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute response of the thyroid to external radiation. 甲状腺对外部辐射的急性反应。

Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement Pub Date : 1983-01-01

I Holten

引用次数: 0

Myxofibrosarcoma. A malignant soft tissue tumor of fibroblastic-histiocytic origin. A clinicopathologic and prognostic study of 110 cases using multivariate analysis. Myxofibrosarcoma。源自成纤维细胞-组织细胞的恶性软组织肿瘤。对110例临床病理及预后进行多因素分析。

Acta pathologica, microbiologica, et immunologica Scandinavica. Supplement Pub Date : 1983-01-01

C Merck, L Angervall, L G Kindblom, A Odén

引用次数: 0