The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry最新文献
{"title":"Chloride-ATPase dephosphorylation in Aplysia gut.","authors":"G. Gerencser, Jianliang Zhang","doi":"10.1002/JEZ.10098","DOIUrl":"https://doi.org/10.1002/JEZ.10098","url":null,"abstract":"The present study was done primarily to compare cation-ATPase dephosphorylation kinetics with a Cl(-)-ATPase's dephosphorylation kinetics because of the paucity of information in this area. Utilizing a proteoliposomal preparation containing Cl(-)-ATPase from Aplysia gut, it was demonstrated that dephosphorylation of this P-type ATPase was absolutely dependent upon Cl(-). Adenosine triphosphate (ATP) concentrations directly stimulated dephosphorylation of Cl(-)-ATPase in the presence of increasing concentrations of Cl(-). It was also shown that the calculated rate constant for E(1)-P disintegration was 20/sec. This rate constant value approximated E(1)-P rate constant disintegration values for other electrogenic, uniport P-type ATPases. Therefore, it was concluded from these results that the Cl(-)-ATPase dephosphorylation kinetics did not differ greatly from cation-ATPase dephosphorylation kinetics.","PeriodicalId":77637,"journal":{"name":"The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry","volume":"37 1","pages":"89-93"},"PeriodicalIF":0.0,"publicationDate":"2002-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90219709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Maternally derived yolk hormones vary in follicles of the painted turtle, Chrysemys picta.","authors":"R. Bowden, M. Ewert, Steven Freedberg, C. Nelson","doi":"10.1002/JEZ.10094","DOIUrl":"https://doi.org/10.1002/JEZ.10094","url":null,"abstract":"The transfer of hormones from a female to her offspring is known to occur in egg laying vertebrates, and the potential for these early, maternally derived hormones to influence sex determination in reptiles with temperature-dependent sex determination is intriguing. In the present study, we examine variation in the concentrations of progesterone, testosterone, and estradiol among three follicle size classes within a female painted turtle (Chrysemys picta) and among females across four periods that span the pre- to post-nesting season. Females were collected, and both follicles and shelled eggs (when present) were harvested for hormone analysis. Progesterone levels did not vary seasonally. However, the concentration of progesterone did vary among and within follicle classes, and was primarily dependent upon ovulatory state: Recently ovulated follicles (as yolks within shelled eggs) contained significantly more progesterone than unovulated follicles. Concentrations of testosterone were low and did not vary either among size classes or across the season. Estradiol levels decreased with increasing follicle size and were higher later in the nesting season. Thus, hormone concentrations varied among follicle sizes and states but in patterns that differed among hormones. This variation has the potential to influence sex determination.","PeriodicalId":77637,"journal":{"name":"The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry","volume":"27 1","pages":"67-72"},"PeriodicalIF":0.0,"publicationDate":"2002-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83431800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Acceleration of Ambystoma tigrinum metamorphosis by corticotropin-releasing hormone.","authors":"G. Boorse, R. Denver","doi":"10.1002/JEZ.10115","DOIUrl":"https://doi.org/10.1002/JEZ.10115","url":null,"abstract":"Previous work of others and ours has shown that corticotropin-releasing hormone (CRH) is a positive stimulus for thyroid and interrenal hormone secretion in amphibian larvae and that activation of CRH neurons may mediate environmental effects on the timing of metamorphosis. These studies have investigated CRH actions in anurans (frogs and toads), whereas there is currently no information regarding the actions of CRH on metamorphosis of urodeles (salamanders and newts). We tested the hypothesis that CRH can accelerate metamorphosis of tiger salamander (Ambystoma tigrinum) larvae. We injected tiger salamander larvae with ovine CRH (oCRH; 1 microg/day; i.p.) and monitored effects on metamorphosis by measuring the rate of gill resorption. oCRH-injected larvae completed metamorphosis earlier than saline-injected larvae. There was no significant difference between uninjected and saline-injected larvae. Mean time to reach 50% reduction in initial gill length was 6.9 days for oCRH-injected animals, 11.9 days for saline-injected animals, and 14.1 days for uninjected controls. At the conclusion of the experiment (day 15), all oCRH-injected animals had completed metamorphosis, whereas by day 15, only 50% of saline-injected animals and 33% of uninjected animals had metamorphosed. Our results show that exogenous oCRH can accelerate metamorphosis in urodele larvae as it does in anurans. These findings suggest that the neuroendocrine mechanisms controlling metamorphosis are evolutionarily conserved across amphibian taxa.","PeriodicalId":77637,"journal":{"name":"The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry","volume":"383 1","pages":"94-8"},"PeriodicalIF":0.0,"publicationDate":"2002-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80718642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zandong Li, Yonghong Song, Yuzhong Ma, Hua Wei, Chun-Hsu Liu, Jinsong Huang, Ning Wang, J. Sha, Fujiro Sakurai
{"title":"Influence of simulated microgravity on avian primordial germ cell migration and reproductive capacity.","authors":"Zandong Li, Yonghong Song, Yuzhong Ma, Hua Wei, Chun-Hsu Liu, Jinsong Huang, Ning Wang, J. Sha, Fujiro Sakurai","doi":"10.1002/JEZ.10088","DOIUrl":"https://doi.org/10.1002/JEZ.10088","url":null,"abstract":"Fertilized eggs of chicken and quail were incubated under the simulated microgravity condition provided by a clinostat. The number of Primordial Germ Cells (PGCs) was counted in early embryogenesis, and the reproductive capacity of quail hatched following the simulated microgravity was investigated. Simulated microgravity caused significant decline of PGCs in the blood of early chicken embryos and in the gonads. The numbers of spermatogonia in the hatchling testis were also fewer than those in the control groups. Therefore, simulated microgravity may retard gonadial development and reduce the reproductive capacity.","PeriodicalId":77637,"journal":{"name":"The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry","volume":"15 1","pages":"672-6"},"PeriodicalIF":0.0,"publicationDate":"2002-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76856520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Bermúdez-Humarán, A. García-García, C. Leal-Garza, V. Riojas-Valdés, G. Jaramillo‐Rangel, R. Montes-de-Oca-Luna
{"title":"Molecular sexing of monomorphic endangered Ara birds.","authors":"L. Bermúdez-Humarán, A. García-García, C. Leal-Garza, V. Riojas-Valdés, G. Jaramillo‐Rangel, R. Montes-de-Oca-Luna","doi":"10.1002/JEZ.10070","DOIUrl":"https://doi.org/10.1002/JEZ.10070","url":null,"abstract":"Survival of most endangered birds may depend on breeding programs where sex identification plays an important role. Molecular sexing has shown to be a rapid and safe procedure. In this work we established sex identification of monomorphic endangered Ara birds using a chromosome W-linked DNA marker, the Chromo-helicase-DNA-Binding 1 (CHD) gene. Most birds have two CHD sex-linked genes, one W-linked (CHD-W) and one Z-linked (CHD-Z). These markers were characterized from Ara militaris and gender sex was determined by PCR and restriction analyzes. The procedure here reported was successfully applied to five different species of the genus Ara and confirmed the validity of the technique. To our knowledge, this is the first report of molecular sexing of the Ara species. This molecular sexing is currently been used in breeding programs of Ara birds.","PeriodicalId":77637,"journal":{"name":"The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry","volume":"22 1","pages":"677-80"},"PeriodicalIF":0.0,"publicationDate":"2002-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73335461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Costs of anorexia during pregnancy in a viviparous snake (Vipera aspis).","authors":"O. Lourdais, X. Bonnet, P. Doughty","doi":"10.1002/JEZ.10065","DOIUrl":"https://doi.org/10.1002/JEZ.10065","url":null,"abstract":"Spontaneous anorexia has been documented in various animal species and is usually associated with activities competing with food intake. In natural conditions, most female aspic vipers (Vipera aspis) stop feeding during the two months of pregnancy. We carried out a simple experiment on 40 pregnant females to determine whether anorexia was obligatory or facultative, and to investigate the energetic consequence of fasting on post-partum body condition and litter traits. Three diet treatments were applied during gestation: no food, one feeding occasion, and two feeding occasions. Twelve nonpregnant, unfed females were used as a control group. Most gravid females accepted captive mice during gestation, suggesting that anorexia reported in the field was a side effect of the tremendous changes in activity pattern associated with pregnancy. Mass loss was high for unfed reproductive females, indicating high energy expenditure associated with embryo maintenance. Prey consumption allowed compensation for metabolic expenditure and enhanced post-partum female body condition, but had no effects on litter characteristics. The magnitude of the metabolic expenditure during gestation appeared to be independent of fecundity.","PeriodicalId":77637,"journal":{"name":"The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry","volume":"22 1","pages":"487-93"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86507796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Oviducal sperm storage in turkeys: spatial distribution of sperm within the uterovaginal junction sperm-storage tubules.","authors":"M. Bakst, B. Vinyard","doi":"10.1002/JEZ.1156","DOIUrl":"https://doi.org/10.1002/JEZ.1156","url":null,"abstract":"The spatial distribution of sperm within the sperm storage tubules (SST) found in the uterovaginal junction (UVJ) of the turkey is not known. In this study, we inseminated sperm stained with a fluorescent dye (Hoechst 33342) to determine their distribution in SST in the ventral, dorsal, and lateral regions of the proximal, middle, and distal regions of the UVJ. There was no preferential filling in the ventral-dorsal axis of the UVJ. In contrast, preferential filling of the SST was observed in the middle section of the UVJ. Here the individual SST were clearly longer and more pleomorphic than the SST in the more proximal and distal aspects of the UVJ. While no information on the temporal aspect of SST filling by sperm could be gleaned, it is evident that the more morphologically developed SST either accept sperm more readily or store sperm more efficiently than SST elsewhere in the UVJ.","PeriodicalId":77637,"journal":{"name":"The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry","volume":"42 1","pages":"206-9"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85331903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The evolution of genome size: what can be learned from anuran development?","authors":"A. Chipman, O. Khaner, A. Haas, E. Tchernov","doi":"10.1002/JEZ.1135","DOIUrl":"https://doi.org/10.1002/JEZ.1135","url":null,"abstract":"Differences in nuclear DNA content in vertebrates have been shown to be correlated with cell size, cell division rate, and embryonic developmental rate. We compare seven species of anuran amphibians with a three-fold range of genome sizes. Parameters examined include the number and density of cells in a number of embryonic structures, and the change in cell number in the CNS during development. We show that genome size is correlated with cell proliferation rate and with developmental rate at different stages of embryonic development, but that the correlation between genome size and cell size is only evident at later stages. We discuss the evolution of genome size in amphibians. Our discussion takes into account data that reportedly support two conflicting hypotheses: the \"skeletal DNA\" hypothesis, which claims a selective role for differences in genome size, and the \"junk DNA\" hypothesis, which claims that differences in genome size are a random result of the accumulation of noncoding DNA sequences. We show that these supposedly conflicting hypotheses can be integrated into a more complex and inclusive model for the evolution of genome size.","PeriodicalId":77637,"journal":{"name":"The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry","volume":"109 1","pages":"365-74"},"PeriodicalIF":0.0,"publicationDate":"2001-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72984168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A molecular phylogenetic study of ecological diversification in the Australian lizard genus Ctenophorus.","authors":"J. Melville, J. Schulte, A. Larson","doi":"10.1002/JEZ.1133","DOIUrl":"https://doi.org/10.1002/JEZ.1133","url":null,"abstract":"We present phylogenetic analyses of the lizard genus Ctenophorus using 1,639 aligned positions of mitochondrial DNA sequences containing 799 parsimony-informative characters for samples of 22 species of Ctenophorus and 12 additional Australian agamid genera. Sequences from three protein-coding genes (ND1, ND2, and COI) and eight intervening tRNA genes are examined using both parsimony and maximum-likelihood analyses. Species of Ctenophorus form a monophyletic group with Rankinia adelaidensis, which we suggest placing in Ctenophorus. Ecological differentiation among species of Ctenophorus is most evident in the kinds of habitats used for shelter. Phylogenetic analyses suggest that the ancestral condition is to use burrows for shelter, and that habits of sheltering in rocks and shrubs/hummock grasses represent separately derived conditions. Ctenophorus appears to have undergone extensive cladogenesis approximately 10-12 million years ago, with all three major ecological modes being established at that time.","PeriodicalId":77637,"journal":{"name":"The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry","volume":"19 1","pages":"339-53"},"PeriodicalIF":0.0,"publicationDate":"2001-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73565710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"What is the promise of developmental evolution? Part II: A causal explanation of evolutionary innovations may be impossible.","authors":"Günter P. Wagner","doi":"10.1002/JEZ.1130","DOIUrl":"https://doi.org/10.1002/JEZ.1130","url":null,"abstract":"","PeriodicalId":77637,"journal":{"name":"The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry","volume":"52 1","pages":"305-9"},"PeriodicalIF":0.0,"publicationDate":"2001-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87870509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}