European journal of basic and applied histochemistry最新文献

Flow cytometric nuclear DNA content of fresh and paraffin-embedded tissues of breast carcinomas and fibroadenomas. 乳腺癌和纤维腺瘤新鲜组织和石蜡包埋组织的流式细胞术核DNA含量。

European journal of basic and applied histochemistry Pub Date : 1991-01-01

R De Vita, A Calugi, P Eleuteri, O Maggi, C Nassuato, A Vecchione

{"title":"Flow cytometric nuclear DNA content of fresh and paraffin-embedded tissues of breast carcinomas and fibroadenomas.","authors":"R De Vita, A Calugi, P Eleuteri, O Maggi, C Nassuato, A Vecchione","doi":"","DOIUrl":"","url":null,"abstract":"Flow cytometric analysis of DNA ploidy level in fresh tissue samples of human breast tumors has been carried out extensively. Recently, investigations regarding the prognostic value of nuclear DNA content have been facilitated by using nuclei isolated from paraffin-embedded tissues. The aim of our study was to monitor the possible differences between the analysis in fresh and fixed paraffin-embedded tissues in the same tumor and to investigate the possible prognostic implications obtained with this new approach. Nuclei suspensions were obtained, according to the method proposed by Hedley et al. with minor modifications, from 45 carcinomas and 5 fibroadenomas. Flow cytometric analysis revealed diploidy in 57% of carcinomas, while the remaining 43% showed cytometric aneuploidy. Corresponding results were observed between fresh and paraffin-embedded tissue in 26/35 cases. Moreover, a fairly good correlation between the DNA indices of fresh and paraffin-embedded carcinoma samples was observed. Furthermore, the frequency of recurrence was higher in the aneuploid group. Finally, 4 fibroadenomas were diploid and one was aneuploid. Our results confirm that this approach permits retrospective studies to evaluate the potential prognostic significance of nuclear DNA content monitored by flow cytometry.","PeriodicalId":77535,"journal":{"name":"European journal of basic and applied histochemistry","volume":"35 3","pages":"233-44"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12882474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunocytochemical localization of the storage protein phaseolin in the embryo-suspensor of Phaseolus coccineus. 红豆胚柄中贮藏蛋白phaseolin的免疫细胞化学定位。

European journal of basic and applied histochemistry Pub Date : 1991-01-01

W Nagl, T Seeliger, A Hartmann

引用次数: 0

Nucleolar organizer regions in dysplastic and neoplastic lesions of the large bowel. 大肠发育不良和肿瘤病变的核仁组织区。

European journal of basic and applied histochemistry Pub Date : 1991-01-01

M Muscarà, G Giuffrè, G Tuccari, G Barresi

引用次数: 0

Cytometric environments: from nucleus to tissues. Proceedings of the VIII national meeting of the Italian Cytometry Group. Urbino, 12-14 September 1991. 细胞环境:从细胞核到组织。意大利细胞术小组第八届全国会议论文集。乌尔比诺，1991年9月12日至14日。

European journal of basic and applied histochemistry Pub Date : 1991-01-01

引用次数: 0

Preliminary findings on the nucleus of large neurons in Lophius piscatorius L. (Osteichthyes, Lophiiformes). 鱼纹鱼(Lophius piscatorius L.)大神经元核的初步研究。

European journal of basic and applied histochemistry Pub Date : 1991-01-01

I Benedetti, L Mola

引用次数: 0

Hepatoid gastric adenocarcinoma. A histological and immunohistochemical study of a case. 肝样胃腺癌。一个病例的组织学和免疫组织化学研究。

European journal of basic and applied histochemistry Pub Date : 1991-01-01

R A Caruso

引用次数: 0

Mast cell fixation and staining in image analysis. 图像分析中肥大细胞的固定和染色。

European journal of basic and applied histochemistry Pub Date : 1991-01-01

S Bianchi, L Mugnai

{"title":"Mast cell fixation and staining in image analysis.","authors":"S Bianchi, L Mugnai","doi":"","DOIUrl":"","url":null,"abstract":"Modern image analysers automatically perform densitometric measurements and elaborate digital images. Elaboration however is subject to operator interpretation and often eliminates precious information from the areas of interest. For this reason, it was appropriate to find a staining method which would overcome this drawback and, in the case of mast cell histochemistry, limit staining to granule content. The following current staining techniques were tested: Toluidine Blue in buffered solution (solut. a) and in 0.003% alcoholic solution (solut. b) and alcoholic Astra Blue, pH 0.2 Densitometric analysis was performed on both 5 microns and semithin sections of mouse tongue fixed in Isotonic formaldehyde-acetic acid (IFAA). Digital images were obtained using 630 nm and 546 nm wavelengths for Toluidine Blue and 610 nm for Astra Blue. Direct comparison between the two Toluidine Blue solutions revealed that more pixels were captured by the 5 microns sections stained with solut. a, whilst the opposite occurred in semithin sections. Both dyes introduced a certain amount of error due to the orthochromatic component of the nucleus and cytoplasmic basophily, which had to be eliminated through image elaboration. Because of its subjective nature, this operation may in turn lead to further errors. The choice of Astra Blue as an alternative to Toluidine Blue in densitometric analysis of mastocytes is based on its property to restrict staining to the granules of mast cells. A comparison between Astra Blue and the two Toluidine Blue solutions showed that, at all transmission levels, preparations stained with Astra Blue captured more pixels than those stained with Toluidine Blue. Consequently our results suggest that the most suitable technique for densitometric image analysis is fixation of mast cells in IFAA followed with Astra Blue.","PeriodicalId":77535,"journal":{"name":"European journal of basic and applied histochemistry","volume":"35 2","pages":"161-74"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12886738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors involved in drug interference on enzyme activities of three mitochondrial populations from rat hippocampus. 药物干扰大鼠海马三种线粒体酶活性的相关因素。

European journal of basic and applied histochemistry Pub Date : 1991-01-01

R F Villa, B Ghigini, R Arnaboldi, D Geroldi, A Gorini

{"title":"Factors involved in drug interference on enzyme activities of three mitochondrial populations from rat hippocampus.","authors":"R F Villa, B Ghigini, R Arnaboldi, D Geroldi, A Gorini","doi":"","DOIUrl":"","url":null,"abstract":"The maximal rate (Vmax) of some mitochondrial enzyme activities related to energy transduction (citrate synthase, alpha-ketoglutarate dehydrogenase, malate dehydrogenase, succinate dehydrogenase, NADH-cytochrome c reductase, cytochrome oxidase) and amino acid metabolism (glutamate dehydrogenase, glutamate-pyruvate transaminase and glutamate-oxaloacetate transaminase) are evaluated in non synaptic (\"free\") and intrasynaptic mitochondria from brain hippocampus. The different mitochondrial populations were isolated from rat subjected to single i.p. treatment with saline solution, almitrine (30 mg/kg) and delta-yohimbine (10 mg/kg). In control rats, the mitochondrial populations exhibit different enzymatic patterns. Acute treatment with almitrine decreases cytochrome oxidase activity in intra-synaptic mitochondria, while acute treatment with delta-yohimbine decreases succinate dehydrogenase activity in both types of free and intra-synaptic mitochondria. NADH-cytochrome c reductase activity is also decreased by acute treatment with almitrine (\"free\" and \"synaptic\" mitochondria) and delta-yohimbine (synaptic mitochondria only).","PeriodicalId":77535,"journal":{"name":"European journal of basic and applied histochemistry","volume":"35 4","pages":"409-21"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12963477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oviduct ampullary epithelium contributes a glycoprotein to the zona pellucida, perivitelline space and blastomeres membrane of sheep embryos. 绵羊胚胎输卵管壶腹上皮为透明带、卵泡周间隙和卵裂球膜提供糖蛋白。

European journal of basic and applied histochemistry Pub Date : 1991-01-01

F Gandolfi, S Modina, T A Brevini, C Galli, R M Moor, A Lauria

{"title":"Oviduct ampullary epithelium contributes a glycoprotein to the zona pellucida, perivitelline space and blastomeres membrane of sheep embryos.","authors":"F Gandolfi, S Modina, T A Brevini, C Galli, R M Moor, A Lauria","doi":"","DOIUrl":"","url":null,"abstract":"Previous studies have identified a glycoprotein (sOP 92) that is secreted by sheep oviductal epithelium and subsequently becomes associated with developing embryos. The objective of this study was to more definitively demonstrate the localization of this protein in the oviductal epithelium of cycling ewes and in the embryos (developmental stage ranging between 1 and 8 cells) by using the light and electron microscopic immuno-gold techniques. In the oviduct, sOP 92 was present only in nonciliated cells of the ampulla but not in the isthmus. No major differences in signal intensity, in its organ or intracellular distribution were observed between the postovulatory and the luteal phase of the cycle. At the embryonic level, sOP 92 uniformly covered the surface of the zona pellucida and permeated its width. Moreover, the protein was present in the perivitelline space and on the blastomeres membrane but not in the cytoplasm or in the nucleus. These observations suggest that a marked regional difference in sheep oviduct secretions exists and confirm that there is a close association of these secretions with the developing embryos.","PeriodicalId":77535,"journal":{"name":"European journal of basic and applied histochemistry","volume":"35 4","pages":"383-92"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12963475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Static cytometry of DNA in breast lump with reference to grading. 乳腺肿块DNA的静态细胞计数及分级。

European journal of basic and applied histochemistry Pub Date : 1991-01-01

P Grigolato, E Valagussa, C Chioda, C Donzelli

引用次数: 0