Advances in biotechnological processes最新文献

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Genetic engineering of human interferons from lymphoblastoid cells: I. Cloning of interferon species expressed in Sendai-induced Namalva cells. 人淋巴母细胞干扰素的基因工程:1 .仙台诱导的Namalva细胞中干扰素表达的克隆。
S Cohen, B Velan, A Shafferman
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引用次数: 0
Blood fractionation: proteins. 血液分离:蛋白质。
M H Stryker, M J Bertolini, Y L Hao
{"title":"Blood fractionation: proteins.","authors":"M H Stryker, M J Bertolini, Y L Hao","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77482,"journal":{"name":"Advances in biotechnological processes","volume":"4 ","pages":"275-336"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15018087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Culture media for propagation of mammalian cells, viruses, and other biologicals. 用于哺乳动物细胞、病毒和其它生物制品繁殖的培养基。
D W Jayme, K E Blackman
{"title":"Culture media for propagation of mammalian cells, viruses, and other biologicals.","authors":"D W Jayme,&nbsp;K E Blackman","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this brief review, we have illustrated the historical development of the growth media commonly employed for the propagation of cultured mammalian cells. While substantial progress has been achieved, the field may best be described as conservative and pragmatic. To date, the function of many components of the growth medium essential for cellular proliferation and biological production has not been precisely defined at the molecular level. Thus, for most large-scale biological production requirements, as well as for routine cell culture and bench-scale pilot development, the traditional enriched culture medium supplemented with fetal bovine serum represents the most convenient culture system. Many cell types may be more economically grown without reduction in biological yield by substituting alternative mammalian sera. Where reduction of total protein or greater definition of growth medium components outweighs the use of more universally applicable culture media, substitution of serum-free, customized formulations of highly enriched growth medium plus defined growth factors may be of significant utility. Optimization of mammalian cell culture media for large-scale biological production should include the following: An initial time investment to optimize the cell culture medium by enriching intermediary metabolite composition (rather than expecting serum or additional growth factors to perform nutritional functions) may result in higher productivity and reduced cost. When screening potential growth media for biological production applications, proliferative rate should not be the sole criterion for performance. Although rapid, logarithmic growth is advantageous to establish large-scale cultures, the maximal cell density and duration of the viable, productive period must also be weighed. Many cell types generate the highest titers of biological product either at stationary phase or under mildly stressful (\"controlled death\") conditions suboptimal for cellular replication. Thus, the ultimate determinant of growth medium efficacy is neither the degree of definition of medium composition nor the cellular proliferative rate, but the ability to support synthesis of substantial titers of the desired product at reasonably high purity.</p>","PeriodicalId":77482,"journal":{"name":"Advances in biotechnological processes","volume":"5 ","pages":"1-30"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13565205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic engineering of human interferons from lymphoblastoid cells: II. Construction of trp expression plasmids and their use for efficient expression of IFN-alpha J1. 人淋巴母细胞样细胞干扰素的基因工程研究II。trp表达质粒的构建及其在ifn - α J1高效表达中的应用。
A Shafferman, S Cohen, H Grosfeld, M Leitner, B Velan
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引用次数: 0
Production of plasminogen activator by cells in culture. 细胞在培养中产生纤溶酶原激活剂。
A Kadouri, Z Bohak
{"title":"Production of plasminogen activator by cells in culture.","authors":"A Kadouri,&nbsp;Z Bohak","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77482,"journal":{"name":"Advances in biotechnological processes","volume":"5 ","pages":"275-99"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15024209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioinsecticides: I. Bacillus thuringiensis. 生物杀虫剂:1 .苏云金芽孢杆菌。
A Krieg, H G Miltenburger
{"title":"Bioinsecticides: I. Bacillus thuringiensis.","authors":"A Krieg,&nbsp;H G Miltenburger","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77482,"journal":{"name":"Advances in biotechnological processes","volume":"3 ","pages":"273-90"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17458623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioinsecticides: II. Baculoviridae. 生物杀虫剂:II。杆状病毒科。
H G Miltenburger, A Krieg
{"title":"Bioinsecticides: II. Baculoviridae.","authors":"H G Miltenburger,&nbsp;A Krieg","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77482,"journal":{"name":"Advances in biotechnological processes","volume":"3 ","pages":"291-313"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17458624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacillus sphaericus: microbiological factors related to its potential as a mosquito larvicide. 球形芽孢杆菌:与杀蚊幼虫潜能有关的微生物因素。
A A Yousten
{"title":"Bacillus sphaericus: microbiological factors related to its potential as a mosquito larvicide.","authors":"A A Yousten","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77482,"journal":{"name":"Advances in biotechnological processes","volume":"3 ","pages":"315-43"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17217705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibody-toxin conjugates: a new approach to cancer therapy. 抗体-毒素结合物:癌症治疗的新途径。
D P McIntosh
{"title":"Antibody-toxin conjugates: a new approach to cancer therapy.","authors":"D P McIntosh","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77482,"journal":{"name":"Advances in biotechnological processes","volume":"3 ","pages":"111-39"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17458619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The preservation of genetically unstable microorganisms and the cryopreservation of fermentation seed cultures. 遗传不稳定微生物的保存和发酵种子培养物的低温保存。
R J Sharp
{"title":"The preservation of genetically unstable microorganisms and the cryopreservation of fermentation seed cultures.","authors":"R J Sharp","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77482,"journal":{"name":"Advances in biotechnological processes","volume":"3 ","pages":"81-109"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17458627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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