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Acta biochimica et biophysica Hungarica最新文献

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Regulation of cellular polyamines in mammals. 哺乳动物细胞多胺的调控。
Acta biochimica et biophysica Hungarica Pub Date : 1988-01-01
N Seiler, O Heby
{"title":"Regulation of cellular polyamines in mammals.","authors":"N Seiler, O Heby","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77479,"journal":{"name":"Acta biochimica et biophysica Hungarica","volume":"23 1","pages":"1-35"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14269182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Low temperature restriction of charge shift in the primary reaction of the bacteriorhodopsin photocycle. 低温对细菌视紫红质光循环初级反应中电荷转移的限制。
Acta biochimica et biophysica Hungarica Pub Date : 1988-01-01
A K Dioumaev, L Keszthelyi
{"title":"Low temperature restriction of charge shift in the primary reaction of the bacteriorhodopsin photocycle.","authors":"A K Dioumaev,&nbsp;L Keszthelyi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fast electrical signals associated with the primary photoreaction of the bacteriorhodopsin photocycle were studied on dried oriented samples in the temperature range from 77 to 300 K. The rise of the electrical signal, associated with bathointermediate formation, was faster than 5 ns even at 77 K; no slow rising component was detected at any temperature in the nano- to microsecond time range. The amplitude of the signal associated with bathointermediate formation was not affected by cooling from 300 to 210 K, but decreased by a factor of two when the sample was further cooled from 210 to 190 K. At 77 K the amplitude from the first excitation flash is 25-30 per cent of that at 260 K. Our data suggest that low temperature restricts the size of the charge shift during the bathointermediate formation, resulting in creation of a \"low temperature bathointermediate\" distinct from the \"room temperature bathointermediate\".</p>","PeriodicalId":77479,"journal":{"name":"Acta biochimica et biophysica Hungarica","volume":"23 3-4","pages":"271-8"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14281220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Summation of unit potentials. 单位势的和。
Acta biochimica et biophysica Hungarica Pub Date : 1988-01-01
G Bíró, S Illyés, J Tóth
{"title":"Summation of unit potentials.","authors":"G Bíró,&nbsp;S Illyés,&nbsp;J Tóth","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The analysis of the compound surface EMG records is directed to obtain information on the activity of motor units. The aim of this study is to contribute to the investigation of the processes taking place in the summation of different unit potentials. The experiments were performed on frog's nerve-muscle preparations. The nerves were stimulated separately and collectively with synchronous and asynchronous electrical impulses. The results obtained by the analysis of the action potentials recorded from the muscles demonstrate that the compound action potentials were brought about by the linear summation of unit potentials involved in the common response.</p>","PeriodicalId":77479,"journal":{"name":"Acta biochimica et biophysica Hungarica","volume":"23 3-4","pages":"255-64"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14281218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of aeration on cortisol and androgen output by pig adrenal homogenate. 曝气对猪肾上腺匀浆分泌皮质醇和雄激素的影响。
Acta biochimica et biophysica Hungarica Pub Date : 1988-01-01
L Kecskés, E Palotás, Z Juricskay, L Királyfalvi, M Szécsényi, K Nemes
{"title":"Effect of aeration on cortisol and androgen output by pig adrenal homogenate.","authors":"L Kecskés,&nbsp;E Palotás,&nbsp;Z Juricskay,&nbsp;L Királyfalvi,&nbsp;M Szécsényi,&nbsp;K Nemes","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Of the steroids produced by pig adrenal homogenate, the output of cortisol+11-deoxycortisol, corticosterone, 11 beta-hydroxyandrostenedione, and the steroid intermediates: pregnenolone, 17 alpha-hydroxypregnenolone, progesterone, 17 alpha-hydroxyprogesterone were analysed. The incubations were carried out with and without aeration. Without aeration the oxygen tension in assay medium decreased during incubation in spite of constant shaking. However, it remained constant (140 Hgmm) with aeration throughout the incubation (60 min). The results of steroid analysis demonstrated that the output of 11 beta-hydroxyandrostenedione decreased strongly, and that of cortisol slightly without aeration, however, the output of corticosterone did not change. The addition of 17 alpha-hydroxypregnenolone or 17 alpha-hydroxyprogesterone to the incubation medium eliminated the decrease in the output of cortisol, but not that of 11 beta-hydroxyandrostenedione. The observations suggest that the activation of oxygen in the enzyme reaction catalyzed by 17, 20-lyase, and perhaps by 17 alpha-hydroxylase is weaker than the activation mediated by 21-hydroxylase and 11 beta-hydroxylase.</p>","PeriodicalId":77479,"journal":{"name":"Acta biochimica et biophysica Hungarica","volume":"23 1","pages":"37-47"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14269184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of the R46 and PKM101 plasmids on the repair of DNA. R46和PKM101质粒对DNA修复的影响。
Acta biochimica et biophysica Hungarica Pub Date : 1988-01-01
F Hernádi, I Francia
{"title":"Effect of the R46 and PKM101 plasmids on the repair of DNA.","authors":"F Hernádi,&nbsp;I Francia","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77479,"journal":{"name":"Acta biochimica et biophysica Hungarica","volume":"23 1","pages":"83-102"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14269186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of ligand induced relative change in the bimolecular quenching constant of protein fluorescence: applications to lysozyme and adenosine deaminase. 配体诱导蛋白荧光双分子猝灭常数相对变化的评价:在溶菌酶和腺苷脱氨酶中的应用。
Acta biochimica et biophysica Hungarica Pub Date : 1988-01-01
B Somogyi, J Matkó, A Rosenberg
{"title":"Evaluation of ligand induced relative change in the bimolecular quenching constant of protein fluorescence: applications to lysozyme and adenosine deaminase.","authors":"B Somogyi,&nbsp;J Matkó,&nbsp;A Rosenberg","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>By using our model, described in the preceding paper, we investigate the effect of tri-N-acetylglucosamine binding on lysozyme. Furthermore, we reprocess the recently published data (Biochemistry, 1985, 24, 1342) on the effect of different inhibitors on adenosine deaminase. For lysozyme, the inhibitor binding decreases the dynamic accessibility of Trp-108 by changing the dynamics of the protein region separating the buried Trp-108 from the solvent. The reprocessed data on adenosine deaminase-inhibitor systems indicate that the inhibitors which presumably stabilize different (ground or transient) states alter the protein dynamics in both a qualitatively and quantitatively different manner in good agreement with the thermodynamic data of inhibitor binding. Our approach allows us to conclude that ligand induced changes of protein dynamics are not uniform and usually depend on where the protein-ligand complex is situated along the reaction coordinate (or phase-space) and are not localized to the protein groups building up the binding center.</p>","PeriodicalId":77479,"journal":{"name":"Acta biochimica et biophysica Hungarica","volume":"23 2","pages":"135-48"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14279292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vanadate stimulated NADH oxidation in sarcoplasmic reticulum membrane. 钒酸盐刺激肌浆网膜NADH氧化。
Acta biochimica et biophysica Hungarica Pub Date : 1988-01-01
E Molnár, Z Kiss, L Dux, F Guba
{"title":"Vanadate stimulated NADH oxidation in sarcoplasmic reticulum membrane.","authors":"E Molnár,&nbsp;Z Kiss,&nbsp;L Dux,&nbsp;F Guba","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Vanadate stimulated NADH oxidation was detected in sarcoplasmic reticulum membrane preparations. The reaction showed enzymatic character, with half maximal activating concentration of 1.2 mM vanadate and maximal NADH oxidation 50 nmol/mg protein/minute. Acidic pH, micromolar free Ca2+ concentration and decavanadate addition increased the rate of NADH oxidation. The described enzyme activity is similar to the ones observed in erythrocyte liver and cardiac plasma membranes. The vanadate stimulated NADH oxidation in sarcoplasmic reticulum preparations does not seem to originate from other contaminating membrane elements. The presence of this enzyme activity in the sarcoplasmic reticulum should be taken into consideration when planning experiments with vanadate, especially when measuring ATPase activity through NADH oxidation with coupled enzymatic assay.</p>","PeriodicalId":77479,"journal":{"name":"Acta biochimica et biophysica Hungarica","volume":"23 1","pages":"63-74"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14106719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Defective myo-inositol-1-phosphate synthase production in an inositolless double mutant Neurospora crassa strain. 无肌醇双突变株粗神经孢子菌肌醇-1-磷酸合成酶产生缺陷。
Acta biochimica et biophysica Hungarica Pub Date : 1988-01-01
A Kiss, M Schablik, A Zsindely, G Szabó
{"title":"Defective myo-inositol-1-phosphate synthase production in an inositolless double mutant Neurospora crassa strain.","authors":"A Kiss,&nbsp;M Schablik,&nbsp;A Zsindely,&nbsp;G Szabó","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A slow growing inl+/- mutant was isolated from an inositol dependent (inl) Neurospora crassa strain. The latter strain produces defective myo-inositol-1-phosphate synthase which has residual activity. Inositol, similarly to that found in wild and inl mutant strains, represses the enzyme production in the inl+/- strain as well. Withdrawing inositol from the medium results in derepression of the enzyme synthesis. Derepression is hindered by cycloheximide. Inl+/- character in the double mutant is brought about by overproduction of the defective myo-inositol-1-phosphate synthase.</p>","PeriodicalId":77479,"journal":{"name":"Acta biochimica et biophysica Hungarica","volume":"23 3-4","pages":"215-23"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14111885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Activation/dephosphorylation of rabbit muscle glycogen synthase by the catalytic subunits of protein phosphatase-1 and 2A. 蛋白磷酸酶-1和2A催化亚基激活/去磷酸化兔肌糖原合成酶。
Acta biochimica et biophysica Hungarica Pub Date : 1988-01-01
I Farkas, B Tóth, G Vereb, C Csortos, P Gergely
{"title":"Activation/dephosphorylation of rabbit muscle glycogen synthase by the catalytic subunits of protein phosphatase-1 and 2A.","authors":"I Farkas,&nbsp;B Tóth,&nbsp;G Vereb,&nbsp;C Csortos,&nbsp;P Gergely","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Glycogen synthase a was purified from rabbit skeletal muscle by a procedure involving heparin-Sepharose chromatography. Glycogen synthase a was phosphorylated by the catalytic subunit of cAMP-dependent protein kinase to give synthase b1. Dephosphorylation and activation of synthase b1 was investigated using the catalytic subunits of protein phosphatase-1 and 2A. The dephosphorylation and activation of synthase b1 was biphasic with a larger rate constant for the initial phase. Analysis of tryptic phosphopeptides of glycogen synthase during the course of dephosphorylation revealed a faster initial phosphate release from site-2 by both phosphatases comparing to sites-1a and 1b. Ligand effects on synthase phosphatase reactions were also studied. Spermine was found to inhibit protein phosphatase-1 activity and to stimulate type-2A phosphatase using synthase b1 as substrate.</p>","PeriodicalId":77479,"journal":{"name":"Acta biochimica et biophysica Hungarica","volume":"23 3-4","pages":"231-46"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13992403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
5-fluorodeoxyuridine decreases the effect of arabinofuranosyl-cytosine in undifferentiated human B lymphocytes. 5-氟脱氧尿苷降低阿拉伯糖氟脲基胞嘧啶在未分化人B淋巴细胞中的作用。
Acta biochimica et biophysica Hungarica Pub Date : 1988-01-01
T Spasokukotskaja, J Taljanidisz, M Benczur, M Staub
{"title":"5-fluorodeoxyuridine decreases the effect of arabinofuranosyl-cytosine in undifferentiated human B lymphocytes.","authors":"T Spasokukotskaja,&nbsp;J Taljanidisz,&nbsp;M Benczur,&nbsp;M Staub","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An activated lymphocyte population was isolated from tonsils of 3-6 years old children by density gradient centrifugation. The isolated light density (LD) cells were bearing early B lymphocyte markers and were 5-6 times more active in DNA synthesis than the high density (HD) lymphocytes. It was found that about 70% of CdR was deaminated and converted into dTMP. The cells were very sensitive to araC, but the incorporation of [14C] CdR was twice more sensitive to araC than that of [5-3H]CdR. This effect can be explained by the interconversion of araC into araT nucleotide via the CdR interconversion pathway. This suggestion was also supported by the effect of 5-F-UdR, which decreased the inhibition of DNA synthesis caused by araC. 5-FUdR the inhibitor of thymidylate synthase, possibly decreases the interconversion of araC into araTTP, and its action via a smaller dTTP pool of the cells. Our data suggest, that the sensitivity of cells to araC may depend on the capacity of the CdR----TdR interconversion pathway, which, however, changes during the normal differentiation process of lymphocytes. Thus, the sensitivity of different malignant cells to araC is determined by the differentiation stage at which they were arrested during cell transformation.</p>","PeriodicalId":77479,"journal":{"name":"Acta biochimica et biophysica Hungarica","volume":"23 2","pages":"159-73"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14110326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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