Scandinavian journal of immunology. Supplement最新文献

The detection of human papillomaviruses in cervical biopsies by immunohistochemistry and in situ hybridization. 免疫组织化学和原位杂交技术检测宫颈活检中人乳头瘤病毒。

Scandinavian journal of immunology. Supplement Pub Date : 1992-01-01 DOI: 10.1111/j.1365-3083.1992.tb01623.x

P Shepherd, D Lunny, R Brookes, T Palmer, D McCance

{"title":"The detection of human papillomaviruses in cervical biopsies by immunohistochemistry and in situ hybridization.","authors":"P Shepherd, D Lunny, R Brookes, T Palmer, D McCance","doi":"10.1111/j.1365-3083.1992.tb01623.x","DOIUrl":"https://doi.org/10.1111/j.1365-3083.1992.tb01623.x","url":null,"abstract":"The presence of human papillomavirus (HPV) types 6, 16 and 18 in cervical biopsies can be detected by an immunoperoxidase technique using type-restricted monoclonal antibodies raised against fusion proteins representing the L1 major capsid proteins of these three HPV types. In a retrospective study (n = 54) we have used these antibodies and biotinylated DNA probes of HPV 6, 16 and 18 to detect and type HPV in formalin-fixed material from the cervix. The biopsies were classified histologically into normals, wart infections without dysplasia, cervical intraepithelial neoplasia (CIN) and squamous cell carcinomas. Antibody staining showed that 22% of all CIN was positive for HPV 16 and 40% of cervical warts were positive for HPV 6, 16 and 18. There was no HPV capsid protein detected in the normals and squamous cell carcinomas using these antibodies, whereas 25% of the tumours were positive for HPV 16 by in situ hybridization. Sections of cervical warts and CIN positive for HPV types by in situ hybridization were also positive by antibody staining which suggests that both techniques are detecting replicating virus. We feel these two techniques complement each other in detection and typing of HPV in cervical biopsies from patients with active disease.","PeriodicalId":77377,"journal":{"name":"Scandinavian journal of immunology. Supplement","volume":"11 ","pages":"69-74"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3083.1992.tb01623.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12498904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Anaplasmosis in Uganda. I. Use of dried blood on filter paper and serum samples for serodiagnosis of anaplasmosis--a comparative study. 乌干达的无形体病。1 .使用滤纸上的干血和血清样本进行无形体病血清诊断的比较研究。

Scandinavian journal of immunology. Supplement Pub Date : 1992-01-01 DOI: 10.1111/j.1365-3083.1992.tb01630.x

G S Ssenyonga, S Montenegro-James, I Kakoma, R Hansen

{"title":"Anaplasmosis in Uganda. I. Use of dried blood on filter paper and serum samples for serodiagnosis of anaplasmosis--a comparative study.","authors":"G S Ssenyonga, S Montenegro-James, I Kakoma, R Hansen","doi":"10.1111/j.1365-3083.1992.tb01630.x","DOIUrl":"https://doi.org/10.1111/j.1365-3083.1992.tb01630.x","url":null,"abstract":"The suitability of blood collected on filter papers in comparison with corresponding conventional serum samples in the diagnosis of bovine anaplasmosis was studied using the Complement Fixation Test (CFT), DOT-ELISA, Western immunoblot and Rapid Card Agglutination Test (RCAT). Dried blood on Whatman filter paper no. 1 was eluted in 1.8 ml of PBS 0.05% Tween 20 given an initial dilution of 1:100. The reactivity in both DOT-ELISA and Western immunoblotting was similar to that obtained with the sera diluted 1:100. Filter paper samples gave lower reactivity in all the tests as compared with corresponding serum samples. There was no significant difference in the reactivity between the eluates from filter papers stored at room temperature and those stored at 4 degrees C. Storage at room temperature did not significantly affect reactivity for up to 6 months. Eluates from filter papers stored for 6 months at room temperature continued to give similar reactivity to those from freshly prepared filter papers in both DOT-ELISA and Western blot, and in the Rapid Card Agglutination Test. It is concluded that collecting blood on filter papers is a suitable technique for large-scale screening and for seroepidemiological studies on anaplasmosis, and offers many advantages especially in developing countries where transport and cold chain facilities are a major constraint.","PeriodicalId":77377,"journal":{"name":"Scandinavian journal of immunology. Supplement","volume":"11 ","pages":"103-6"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3083.1992.tb01630.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12682084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Anti-sperm and anti-ovum vaccines: the selection of candidate antigens and the outcome of preclinical studies. 抗精子和抗卵子疫苗:候选抗原的选择和临床前研究的结果。

Scandinavian journal of immunology. Supplement Pub Date : 1992-01-01 DOI: 10.1111/j.1365-3083.1992.tb01633.x

M A Isahakia, C S Bambra

引用次数: 9

Humoral factors of natural resistance of Bos indicus cattle selected for antibody titre to Brucella abortus. 选择牛对流产布鲁氏菌自然抗性的体液因子进行抗体滴度测定。

Scandinavian journal of immunology. Supplement Pub Date : 1992-01-01 DOI: 10.1111/j.1365-3083.1992.tb01629.x

P S Gwakisa, U M Minga

引用次数: 6

Serum, disc and egg ELISA for the serodiagnosis of Salmonella gallinarum and S. enteritidis infections in chickens. 鸡血清、盘和鸡蛋ELISA检测鸡鸡沙门氏菌和肠炎沙门氏菌感染。

Scandinavian journal of immunology. Supplement Pub Date : 1992-01-01 DOI: 10.1111/j.1365-3083.1992.tb01641.x

U M Minga, C Wray, P S Gwakisa

引用次数: 6

Serodiagnosis of bacterial diseases: problems and developments. 细菌性疾病的血清学诊断:问题与进展。

Scandinavian journal of immunology. Supplement Pub Date : 1992-01-01 DOI: 10.1111/j.1365-3083.1992.tb01627.x

W J Terpstra

{"title":"Serodiagnosis of bacterial diseases: problems and developments.","authors":"W J Terpstra","doi":"10.1111/j.1365-3083.1992.tb01627.x","DOIUrl":"https://doi.org/10.1111/j.1365-3083.1992.tb01627.x","url":null,"abstract":"Serodiagnosis is useful when pathogens cannot be detected quickly or easily by conventional methods or have been eliminated by the patient. Taking the example of leptospirosis, the microscopic agglutination test to detect specific antibodies after leptospires have disappeared from the patient's bloodstream is of unsurpassed reliability. Unfortunately the test is used mainly in specialized laboratories that can culture leptospires to serve as live antigens. Freeze-dried leptospires may allow the application of the test on a wide scale. The ELISA is useful for the detection of IgM antibodies in humans as a sign of current or recent infection. ELISA results must be confirmed by the microscopic agglutination test. A simplified easy-to-read ELISA using broadly reactive antigen for the detection of all types of leptospires might be useful for a quick detection of acute human leptospirosis. Using an appropriate antigenic preparation the ELISA is useful for the detection of antibodies in animals, e.g. cattle. As for pathogen detection, DNA-based methods such as PCR are promising for an early, quick and specific diagnosis. Antigenic analysis by monoclonal antibodies and genetic analysis by restriction fragment length polymorphism are for the time being complementary methods for the characterization of leptospiral isolates.","PeriodicalId":77377,"journal":{"name":"Scandinavian journal of immunology. Supplement","volume":"11 ","pages":"91-5"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3083.1992.tb01627.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12682438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Specific IgG subclass antibody levels and phagocytosis of serotype 14 pneumococcus following immunization. 免疫后血清14型肺炎球菌的特异性IgG亚类抗体水平和吞噬作用。

Scandinavian journal of immunology. Supplement Pub Date : 1992-01-01 DOI: 10.1111/j.1365-3083.1992.tb01628.x

A S Kaniuk, J E Lortan, M A Monteil

{"title":"Specific IgG subclass antibody levels and phagocytosis of serotype 14 pneumococcus following immunization.","authors":"A S Kaniuk, J E Lortan, M A Monteil","doi":"10.1111/j.1365-3083.1992.tb01628.x","DOIUrl":"https://doi.org/10.1111/j.1365-3083.1992.tb01628.x","url":null,"abstract":"Complement and specific antibody directed against capsular polysaccharide are necessary for efficient phagocytosis of pneumococci. In normal adults, specific antibody to pneumococci is predominantly of the IgG2 subclass. However, the role of IgG2 in bacterial clearance is debatable. We therefore decided to investigate the relationship between specific IgG subclass antibody levels and phagocytosis of serotype 14 pneumococcus, before and after immunization with a pneumococcal capsular polysaccharide vaccine. Specific IgG subclass antibody was measured by an ELISA technique and the effect of serum on phagocytosis of radiolabelled pneumococci by normal polymorphs was determined. We found that in the presence of complement, phagocytosis correlated significantly with both specific IgG1 and IgG2 antibody titres (r = 0.547, P = 0.002 and r = 0.464, P = 0.009, respectively). However, in decomplemented sera, the correlation with IgG1 antibody was lost, whereas that with IgG2 antibody was strengthened (r = 0.641, P = less than 0.001). The possibility that IgG2 binds to receptors on polymorphs should be considered.","PeriodicalId":77377,"journal":{"name":"Scandinavian journal of immunology. Supplement","volume":"11 ","pages":"96-8"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3083.1992.tb01628.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12682439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Herpes zoster ophthalmicus: an early pointer to HIV-1 positivity in young African patients. 眼部带状疱疹:非洲年轻患者HIV-1阳性的早期指标。

Scandinavian journal of immunology. Supplement Pub Date : 1992-01-01 DOI: 10.1111/j.1365-3083.1992.tb01622.x

G N Palexas, N H Welsh

引用次数: 12

Reduced natural killer cell activity in multi-drug resistant pulmonary tuberculosis. 降低了多重耐药肺结核的自然杀伤细胞活性。

Scandinavian journal of immunology. Supplement Pub Date : 1992-01-01 DOI: 10.1111/j.1365-3083.1992.tb01643.x

L T Ratcliffe, C R Mackenzie, P T Lukey, S R Ress

引用次数: 10

Production, immunoanalysis and selection of monoclonal antibodies for studies of polymorphism of bovine MHC class I antigens. 牛MHCⅰ类抗原单克隆抗体的制备、免疫分析及多态性研究。

Scandinavian journal of immunology. Supplement Pub Date : 1992-01-01 DOI: 10.1111/j.1365-3083.1992.tb01651.x

P S Gwakisa

引用次数: 0