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Research communications - Institute for Fermentation, Osaka最新文献

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Detection of heterogeneity of 18S rRNA inter-genes and mutation arising during PCR amplification. 18S rRNA间基因异质性检测及PCR扩增过程中产生的突变。
Research communications - Institute for Fermentation, Osaka Pub Date : 1997-01-01
K Ueda, K Mikata
{"title":"Detection of heterogeneity of 18S rRNA inter-genes and mutation arising during PCR amplification.","authors":"K Ueda,&nbsp;K Mikata","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Direct sequencing revealed sequence heterogeneity among ribosomal RNA gene (rDNA) operons, consisting of 8 base heterogeneous sites on the 18S rDNA of Galactomyces citri-aurantii IFO 10822, and 6 base heterogeneous sites in the same region on the 18S rDNA of G. citri-aurantii IFO 10821. Sequence analysis of the cloned 18S rRNA genes of 14 species (19 strains) of ascomycetous yeast-like fungi detected a total of 32 substitutions between two cloned sequences from each of 10 strains. Eight substitutions came from heterogeneity of G. citri-aurantii IFO 10822, and 24 substitutions were predicted to be due to misincorporation by the Taq DNA polymerase. A low frequency of random substitution, estimated to occur in PCR at approximately 1 in 2690 nucleotides, was detected; and transitions occurred 7 times more frequently than transversions.</p>","PeriodicalId":77350,"journal":{"name":"Research communications - Institute for Fermentation, Osaka","volume":" 18","pages":"20-5"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20122323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cross-contamination of cell lines as revealed by DNA fingerprinting in the IFO animal cell bank. IFO动物细胞库DNA指纹图谱显示细胞系交叉污染。
Research communications - Institute for Fermentation, Osaka Pub Date : 1993-01-01
M Satoh, M Takeuchi
{"title":"Cross-contamination of cell lines as revealed by DNA fingerprinting in the IFO animal cell bank.","authors":"M Satoh,&nbsp;M Takeuchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>For quality control of cell lines, the Institute for Fermentation, Osaka (IFO) animal cell bank recently introduced DNA fingerprinting analysis, which enables verification of cell lines at the individual level, to detect cross-culture contamination. By using this analysis, we found two cases of cross-contamination of cell lines.</p>","PeriodicalId":77350,"journal":{"name":"Research communications - Institute for Fermentation, Osaka","volume":" 16","pages":"18-23"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18765216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Establishment of HPRT-deficient cell lines from mouse neuroblastoma Neuro-2a and rat pheochromocytoma PC12. 小鼠神经母细胞瘤神经-2a和大鼠嗜铬细胞瘤PC12 hprt缺陷细胞系的建立。
Research communications - Institute for Fermentation, Osaka Pub Date : 1993-01-01
M Satoh, M Takeuchi
{"title":"Establishment of HPRT-deficient cell lines from mouse neuroblastoma Neuro-2a and rat pheochromocytoma PC12.","authors":"M Satoh,&nbsp;M Takeuchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Hypoxanthine/guanine phosphoribosyl transferase (HPRT)-deficient cell lines, designated as Neuro-2aTG and PC12TG, were established from mouse neuroblastoma Neuro-2a and rat pheochromocytoma PC12, respectively. Both cell lines stably exhibited HPRT- phenotype, and expressed neuronal properties, i.e., constitutive expression of 200-kD neurofilament protein in Neuro-2aTG and responsiveness to NGF in PC12TG. Therefore, these cell lines will be useful as fusion partners in somatic cell hybridization with neurons.</p>","PeriodicalId":77350,"journal":{"name":"Research communications - Institute for Fermentation, Osaka","volume":" 16","pages":"6-17"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18765217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of NGF-2 on the survival of chick sensory ganglion neurons in vitro. NGF-2对鸡感觉神经节神经元体外存活的影响。
Research communications - Institute for Fermentation, Osaka Pub Date : 1991-01-01
M Satoh, Y Kaisho, M Takeuchi
{"title":"Effect of NGF-2 on the survival of chick sensory ganglion neurons in vitro.","authors":"M Satoh,&nbsp;Y Kaisho,&nbsp;M Takeuchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The biological activity of NGF-2, the third member of the NGF family, was investigated. Conditioned medium of COS cells transfected with expression plasmid for human NGF-2 cDNA promoted the survival of sensory neurons from dorsal root ganglia (DRG), and nodose ganglia (NG). Supernatant of mock transfected COS cells was less effective for the survival of DRG neurons and ineffective for the survival of NG neurons. These results suggest that NGF-2 is a novel neurotrophic factor whose biological activity is distinct from NGF.</p>","PeriodicalId":77350,"journal":{"name":"Research communications - Institute for Fermentation, Osaka","volume":" 15","pages":"15-21"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12539413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Properties of a cell line (MEG-01SSF) of human megakaryoblastic leukemia cells. 人巨核母细胞白血病细胞系MEG-01SSF的特性研究。
Research communications - Institute for Fermentation, Osaka Pub Date : 1991-01-01
M Takeuchi, M Satoh, M Ogura, H Saito, K Takeuchi
{"title":"Properties of a cell line (MEG-01SSF) of human megakaryoblastic leukemia cells.","authors":"M Takeuchi,&nbsp;M Satoh,&nbsp;M Ogura,&nbsp;H Saito,&nbsp;K Takeuchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>MEG-01SSF cells were prepared from MEG-01S by conditioning in a chemically defined, serum-free medium. The cells grew well in the medium and required transferrin for growth. Doubling time was about 20 hours. The cells retained GpIIb/IIIa as a marker of megakaryocytes and transferrin receptors on the cell surface. Some of the cells in the stationary phase of growth produced platelet-like particles, which bore characteristic microtubule rings.</p>","PeriodicalId":77350,"journal":{"name":"Research communications - Institute for Fermentation, Osaka","volume":" 15","pages":"22-9"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12539414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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