Establishment of HPRT-deficient cell lines from mouse neuroblastoma Neuro-2a and rat pheochromocytoma PC12.

Abstract

Hypoxanthine/guanine phosphoribosyl transferase (HPRT)-deficient cell lines, designated as Neuro-2aTG and PC12TG, were established from mouse neuroblastoma Neuro-2a and rat pheochromocytoma PC12, respectively. Both cell lines stably exhibited HPRT- phenotype, and expressed neuronal properties, i.e., constitutive expression of 200-kD neurofilament protein in Neuro-2aTG and responsiveness to NGF in PC12TG. Therefore, these cell lines will be useful as fusion partners in somatic cell hybridization with neurons.