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PCR methods and applications最新文献

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Rapid quantitative PCR for determination of relative gene expressions in tissue specimens. 快速定量PCR测定组织标本中相关基因表达。
PCR methods and applications Pub Date : 1995-04-01 DOI: 10.1101/gr.4.5.305
H J Lenz, C Hill, K D Danenberg, L L Leichman, D G Priest, P V Danenberg
{"title":"Rapid quantitative PCR for determination of relative gene expressions in tissue specimens.","authors":"H J Lenz, C Hill, K D Danenberg, L L Leichman, D G Priest, P V Danenberg","doi":"10.1101/gr.4.5.305","DOIUrl":"https://doi.org/10.1101/gr.4.5.305","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 5","pages":"305-8"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18586922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Panhandle PCR. 狭长地带PCR。
PCR methods and applications Pub Date : 1995-04-01 DOI: 10.1101/gr.4.5.s195
D H Jones
{"title":"Panhandle PCR.","authors":"D H Jones","doi":"10.1101/gr.4.5.s195","DOIUrl":"https://doi.org/10.1101/gr.4.5.s195","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 5","pages":"S195-201"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18586342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
An efficient method for PCR analysis of mitochondrial DNA from paraffin-embedded archival heart tissue. 石蜡包埋归档心脏组织线粒体DNA的高效PCR分析方法。
PCR methods and applications Pub Date : 1995-04-01 DOI: 10.1101/gr.4.5.309
A Bobba, R Lippolis, S Giannattasio, C Camaschella, E Marra
{"title":"An efficient method for PCR analysis of mitochondrial DNA from paraffin-embedded archival heart tissue.","authors":"A Bobba, R Lippolis, S Giannattasio, C Camaschella, E Marra","doi":"10.1101/gr.4.5.309","DOIUrl":"https://doi.org/10.1101/gr.4.5.309","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 5","pages":"309-10"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18586923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Specific amplification by PCR of rearranged genomic variable regions of immunoglobulin genes from mouse hybridoma cells. 小鼠杂交瘤细胞免疫球蛋白基因基因组可变区重排的PCR特异性扩增。
PCR methods and applications Pub Date : 1995-04-01 DOI: 10.1101/gr.4.5.256
J Berdoz, T P Monath, J P Kraehenbuhl
{"title":"Specific amplification by PCR of rearranged genomic variable regions of immunoglobulin genes from mouse hybridoma cells.","authors":"J Berdoz,&nbsp;T P Monath,&nbsp;J P Kraehenbuhl","doi":"10.1101/gr.4.5.256","DOIUrl":"https://doi.org/10.1101/gr.4.5.256","url":null,"abstract":"<p><p>We have designed a novel strategy for the isolation of the rearranged genomic fragments encoding the L-VH-D-JH and L-V kappa/lambda-J kappa/lambda regions of mouse immunoglobulin genes. This strategy is based on the PCR amplification of genomic DNA from mouse hybridomas using multiple specific primers chosen in the 5'-untranslated region and in the intron downstream of the rearranged JH/J kappa/lambda sequences. Variable regions with intact coding sequences, including full-length leader peptides (L) can be obtained without previous DNA sequencing. Our strategy is based on a genomic template that produces fragments that do not need to be adapted for recombinant antibody expression, thus facilitating the generation of chimeric and isotype-switched immunoglobulins.</p>","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 5","pages":"256-64"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1101/gr.4.5.256","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18586913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Template integrity is essential for PCR amplification of 20- to 30-kb sequences from genomic DNA. 模板完整性是必不可少的PCR扩增20- 30 kb序列从基因组DNA。
PCR methods and applications Pub Date : 1995-04-01 DOI: 10.1101/gr.4.5.294
S Cheng, Y Chen, J A Monforte, R Higuchi, B Van Houten
{"title":"Template integrity is essential for PCR amplification of 20- to 30-kb sequences from genomic DNA.","authors":"S Cheng,&nbsp;Y Chen,&nbsp;J A Monforte,&nbsp;R Higuchi,&nbsp;B Van Houten","doi":"10.1101/gr.4.5.294","DOIUrl":"https://doi.org/10.1101/gr.4.5.294","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 5","pages":"294-8"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1101/gr.4.5.294","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18586919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 60
DNA hybridization analysis of PCR products by non-gel sieving capillary electrophoresis. 非凝胶筛分毛细管电泳PCR产物的DNA杂交分析。
PCR methods and applications Pub Date : 1995-04-01 DOI: 10.1101/gr.4.5.303
M Oto, T Suehiro, Y Yuasa
{"title":"DNA hybridization analysis of PCR products by non-gel sieving capillary electrophoresis.","authors":"M Oto,&nbsp;T Suehiro,&nbsp;Y Yuasa","doi":"10.1101/gr.4.5.303","DOIUrl":"https://doi.org/10.1101/gr.4.5.303","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 5","pages":"303-4"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1101/gr.4.5.303","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18586921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Optimization and troubleshooting in PCR. PCR的优化和故障排除。
PCR methods and applications Pub Date : 1995-04-01 DOI: 10.1101/gr.4.5.s185
K H Roux
{"title":"Optimization and troubleshooting in PCR.","authors":"K H Roux","doi":"10.1101/gr.4.5.s185","DOIUrl":"https://doi.org/10.1101/gr.4.5.s185","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 5","pages":"S185-94"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18586341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 189
Efficient gene synthesis by Klenow assembly/extension-Pfu polymerase amplification (KAPPA) of overlapping oligonucleotides. 利用重叠寡核苷酸的Klenow组装/延伸- pfu聚合酶扩增(KAPPA)高效基因合成。
PCR methods and applications Pub Date : 1995-04-01 DOI: 10.1101/gr.4.5.299
E W Holowachuk, M S Ruhoff
{"title":"Efficient gene synthesis by Klenow assembly/extension-Pfu polymerase amplification (KAPPA) of overlapping oligonucleotides.","authors":"E W Holowachuk,&nbsp;M S Ruhoff","doi":"10.1101/gr.4.5.299","DOIUrl":"https://doi.org/10.1101/gr.4.5.299","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 5","pages":"299-302"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18586920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Amplification of the total coding sequence of the NF1 gene from peripheral blood lymphocyte RNA. 扩增外周血淋巴细胞RNA中NF1基因总编码序列。
PCR methods and applications Pub Date : 1995-04-01 DOI: 10.1101/gr.4.5.311
M H Shen, P S Harper, M Upadhyaya
{"title":"Amplification of the total coding sequence of the NF1 gene from peripheral blood lymphocyte RNA.","authors":"M H Shen,&nbsp;P S Harper,&nbsp;M Upadhyaya","doi":"10.1101/gr.4.5.311","DOIUrl":"https://doi.org/10.1101/gr.4.5.311","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 5","pages":"311-3"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18586924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
In situ PCR: protocols and applications. 原位PCR:方案和应用。
PCR methods and applications Pub Date : 1995-02-01 DOI: 10.1101/gr.4.4.s151
G J Nuovo
{"title":"In situ PCR: protocols and applications.","authors":"G J Nuovo","doi":"10.1101/gr.4.4.s151","DOIUrl":"https://doi.org/10.1101/gr.4.4.s151","url":null,"abstract":"<p><p>Many groups have now published data based on the in situ detection of PCR-amplified DNA and cDNA. As with standard in situ hybridization or PCR, variables that can affect in situ PCR results include type of fixative and time of fixation, protease digestion, and the composition of the amplifying solution and oligoprobe cocktail. Investigators new to the field of in situ PCR should first try direct incorporation of the reporter molecule into paraffin-embedded tissue sections. Although nonspecific DNA synthesis is generated under these conditions, one can develop the confidence of synthesizing DNA inside the nucleus and appreciate the importance of protease digestion time to successful RT in situ PCR. It is an arguable statement that the in situ detection of PCR-amplified DNA and cDNA will have a very strong impact on many diverse fields, such as oncogenesis, embryology, RNA trafficking, and detection of viral diseases, as it already has on our understanding of the pathogenesis of HIV-1 infection.</p>","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 4","pages":"S151-67"},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19554682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 55
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