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Survival of bifidobacteria from human habitat in acidified milk. 人类栖息地双歧杆菌在酸化牛奶中的存活。
Microbiologica Pub Date : 1992-04-01
B Biavati, T Sozzi, P Mattarelli, L D Trovatelli
{"title":"Survival of bifidobacteria from human habitat in acidified milk.","authors":"B Biavati,&nbsp;T Sozzi,&nbsp;P Mattarelli,&nbsp;L D Trovatelli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Some industrial preparations from milk, such as yogurt, contain bifidobacteria as an additional probiotic element. The acidic environment of these products affects the viability of the bifidobacteria. The survival in acidic environment of one-hundred and ten bifidobacterial strains from human habitat was tested.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 2","pages":"197-200"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12769935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of Arthrinium species on several nitrogen sources. 几种氮源上砷属植物的发育。
Microbiologica Pub Date : 1992-04-01
J V Larrondo, A Calvo
{"title":"Development of Arthrinium species on several nitrogen sources.","authors":"J V Larrondo,&nbsp;A Calvo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of this study was to determine the growth aspects of Arthrinium strains in the presence of several nitrogen sources. The addition of tryptone, peptone or soybean peptone to the culture medium stimulated larger colony diameters in most of the isolates.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 2","pages":"201-4"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12769936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibiotic susceptibility patterns surveillance and clinical distribution of Enterobacteriaceae. 肠杆菌科抗生素药敏型监测及临床分布。
Microbiologica Pub Date : 1992-04-01
P Garbagnoli, L Pagani
{"title":"Antibiotic susceptibility patterns surveillance and clinical distribution of Enterobacteriaceae.","authors":"P Garbagnoli,&nbsp;L Pagani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>3700 strains of Enterobacteriaceae clinical isolates were tested by automated devices for susceptibility to several antimicrobial agents widely used in clinical practice. Amikacin demonstrated the greatest in vitro activity whereas ampicillin and mezlocillin were the least active. Finally gentamicin and nalidixic acid had a similar activity to recently introduced cephalosporins. The bacterial species widely isolated were grouped in three clusters according to the clinical body site of isolates: several discrepancies emerged from the study of antibiotic susceptibilities of strains obtained from different body site sources. Source could be correlated with bacterial pattern of resistance.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 2","pages":"135-47"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12770051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of medium salinity on some chemical constituents of two halophilic Bacillus spp. from Saudi Arabia. 培养基盐度对沙特阿拉伯两种嗜盐芽孢杆菌某些化学成分的影响。
Microbiologica Pub Date : 1992-04-01
A A Salamah
{"title":"Effect of medium salinity on some chemical constituents of two halophilic Bacillus spp. from Saudi Arabia.","authors":"A A Salamah","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The cell envelope amino acids of two moderately halophilic Bacillus isolates (BST and BSF) varied according to medium salinity. Cystine and proline were mostly effected. In both isolates growing in the presence of 6 and 18% NaCl there were more dicarboxylic amino acids than basic amino acids which makes the cell envelope proteins quite acidic. The concentrations of the cell-associated cations (Na+, K+, and Mg2+) were high in both isolates, and varied according to the NaCl concentration. The two isolates contained glucosamine and muramic acid in their cell walls. The amounts of these two sugar derivatives, however, varied with the NaCl concentration. Thin-layer chromatography of phospholipids revealed the presence of cardiolipins, phosphatidylglycerols and phosphatidylethanolamines in the two isolates irrespective of medium salinity. Phosphatidylglycerols and the phosphatidylethanolamines increased on increasing the NaCl concentration of the growth medium. Lysophosphatidylglycerols were detected only in the 6% grown BST cells. Unidentified phospholipids designated X1 (in isolates BSF and BST), X2 (in isolate BST) and X3 (in isolate BSF) were also detected; the concentrations of X1 and X3 were salinity dependent.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 2","pages":"149-56"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12769931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phenoloxidase production and vanillic acid metabolism by Zygomycetes. 接合菌的酚氧化酶生产和香草酸代谢。
Microbiologica Pub Date : 1992-04-01
F Seigle-Murandi, P Guiraud, R Steiman, J L Benoit-Guyod
{"title":"Phenoloxidase production and vanillic acid metabolism by Zygomycetes.","authors":"F Seigle-Murandi,&nbsp;P Guiraud,&nbsp;R Steiman,&nbsp;J L Benoit-Guyod","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The ability of 23 strains of Zygomycetes to produce extracellular phenoloxidases was examined on solid media by using 10 different reagents. The results varied depending on the reagent and indicated that most of the strains were devoid of phenoloxidase activity. The production of inducible phenoloxidases was demonstrated by the Bavendamm reaction. The study of the biotransformation of vanillic acid in synthetic medium indicated that the reaction most often obtained was the reduction of vanillic acid to vanillyl alcohol. Helicostylum piriforme and Rhizopus microsporus var. chinensis completely metabolized vanillic acid while good transformation was obtained with Absidia spinosa, Cunninghamella bainieri, Mucor bacilliformis, Mucor plumbeus, Rhizopus arrhizus, Rhizopus stolonifer, Syncephalastrum racemosum and Zygorhynchus moelleri. Other strains did not degrade or poorly degraded vanillic acid. Decarboxylation and demethoxylation of this compound was independent of the production of phenoloxidases as in the case of white-rot fungi. Other enzymatic systems might be implicated in this phenomenon.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 2","pages":"157-65"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12769932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of storage upon the toxicity of synfuel process waters to bacteria. 储存对合成燃料工艺水对细菌毒性的影响。
Microbiologica Pub Date : 1992-04-01
R B Lyon, J C Adams
{"title":"Effect of storage upon the toxicity of synfuel process waters to bacteria.","authors":"R B Lyon,&nbsp;J C Adams","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Storage of synfuel process waters at 4 degrees C for 8 years appeared to reduce the toxicity of these waters to indicator bacteria. When these waters were mixed in the amount of 10 percent process waters to 90 percent sewage, heterotrophic bacteria grew which indicate that storage or aeration may improve the treatability of these waters.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 2","pages":"205-8"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12769937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rotavirus infection in horses. Genome profile analysis of a rotavirus isolated from an infected foal. 马的轮状病毒感染。从感染马驹中分离的轮状病毒的基因组谱分析。
Microbiologica Pub Date : 1992-04-01
R Legrottaglie, P Agrimi
{"title":"Rotavirus infection in horses. Genome profile analysis of a rotavirus isolated from an infected foal.","authors":"R Legrottaglie,&nbsp;P Agrimi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Electrophoretic analysis in polyacrylamide gel (PAGE) of the equine rotavirus 106/88/LI/EQ, isolated from the diarrhea of an 18 day old foal was compared to the bovine strain NCDV. There was a notable difference in the migration of some segments of the viral RNA. Bands 2 and 3 of the equine rotavirus comigrated while there was a clear separation of segments 7, 8 and 9. Moreover, the migration of segments 1, 4 and 5 revealed a lower molecular weight than the corresponding segments of NCDV.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 2","pages":"209-12"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12492780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential cleavage of surface proteins of Borrelia burgdorferi by proteases. 蛋白酶对伯氏疏螺旋体表面蛋白的差异切割。
Microbiologica Pub Date : 1992-04-01
A Moroni, V Sambri, F Massaria, M La Placa, E Brocchi, F De Simone, R Cevenini
{"title":"Differential cleavage of surface proteins of Borrelia burgdorferi by proteases.","authors":"A Moroni,&nbsp;V Sambri,&nbsp;F Massaria,&nbsp;M La Placa,&nbsp;E Brocchi,&nbsp;F De Simone,&nbsp;R Cevenini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The differential cleavage of surface proteins of Borrelia burgdorferi IRS strains by several proteases was examined. Proteinase K, trypsin, chymotrypsin and thermolysin all cleaved the outer surface protein B (OspB) to undetectable levels by Coomassie Brilliant Blue staining, whereas some residual protein was detected by immunoblotting with polyclonal and monoclonal antibodies. Not even antigenic fragments were detectable by immunoblotting with 1A8 monoclonal antibody reactive with OspB. Less effective or ineffective was the cleavage of OspB by V8 protease and proteinase A, respectively. The outer surface protein A was cleaved only by proteinase K. The effect of trypsin on borreliae viability and adhesion to cultured cells was also studied. The trypsin treatment of borreliae did not impair the viability of organisms which continued to synthesize the cleaved OspB. The attachment of B. burgdorferi to HEp-2 cells was reduced by 41% after treatment with trypsin, whereas preincubation of borreliae with monoclonal antibody 1A8 and guinea pig immune serum reduced the adhesion of borreliae to the cells by 32% and 87%, respectively.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 2","pages":"99-106"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12769940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of interferon alpha on murine cytomegalovirus replication. 干扰素对小鼠巨细胞病毒复制的影响。
Microbiologica Pub Date : 1992-04-01
M G Martinotti, G Gribaudo, M Gariglio, A Angeretti, G Cavallo, S Landolfo
{"title":"Effects of interferon alpha on murine cytomegalovirus replication.","authors":"M G Martinotti,&nbsp;G Gribaudo,&nbsp;M Gariglio,&nbsp;A Angeretti,&nbsp;G Cavallo,&nbsp;S Landolfo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present study examined the protective effect of IFN-alpha against mouse cytomegalovirus (MCMV) infection in embryo fibroblasts (MEF) of genetically resistant (C3H/HeJ) and susceptible (C57BL/6) mouse strains. At a M.O.I. of 1 IFN-alpha was protective in C3H/HeJ-MEF but not in C57BL/6-MEF. Dot-blot analysis during MCMV replication in C3H/HeJ-MEF showed that IFN-alpha pretreatment reduced the steady state level of immediate early and late mRNAs but partially reduced early gene expression.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 2","pages":"183-6"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12548573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mice infection with HIV-1: a new mouse model for HIV-1 in vivo research. 小鼠感染HIV-1: HIV-1在体内研究的新小鼠模型。
Microbiologica Pub Date : 1992-04-01
G Filice, P M Cereda, P Orsolini, L Soldini, R Gulminetti, E Romero
{"title":"Mice infection with HIV-1: a new mouse model for HIV-1 in vivo research.","authors":"G Filice,&nbsp;P M Cereda,&nbsp;P Orsolini,&nbsp;L Soldini,&nbsp;R Gulminetti,&nbsp;E Romero","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In mice experimentally infected with 1 x 10(5) UI/mouse of HTLV-IIIB IgM antibodies were detected 10-12 days after the infection, reaching peak values two weeks later; the IgM seratiter progressively decreased thereafter and was negative at ten-eleven weeks. HIV p24 antigen was detected ten-fifteen days after infection and reached peak values five-six weeks later. Antigenemia subsequently decreased and showed an oscillating course with a progressive decrease which persisted throughout the observation period. Two weeks after infection we detected IgG antibodies to the major core protein p24; reactivity to gp41 was observed as early as reactivity to p24 and persisted throughout observation period. The IgG antibodies to all HIV epitopes peaked two-three weeks after infection; the time course showed a decrease after ten weeks, progressively decreasing thereafter. After sixty-five weeks of infection the IgG seratiter value was lower but remained positive. Viruses indistinguishable from HIV were isolated from the peripheral blood mononuclear cells of infected mice 30, 60, 180 days after infection. These seroimmunological and virological data confirm that the immunocompetent mouse may serve as a low-cost reproducible model for HIV-1 in vivo research.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 2","pages":"167-75"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12769933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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