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The effect of microcosm design on the survival of recombinant Pseudomonas putida in lake water. 微环境设计对重组恶臭假单胞菌在湖水中存活的影响。
Microbial releases : viruses, bacteria, fungi Pub Date : 1992-12-01
J A Morgan, G Rhodes, R W Pickup, C Winstanley, J R Saunders
{"title":"The effect of microcosm design on the survival of recombinant Pseudomonas putida in lake water.","authors":"J A Morgan,&nbsp;G Rhodes,&nbsp;R W Pickup,&nbsp;C Winstanley,&nbsp;J R Saunders","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The survival of Pseudomonas putida marked with the xylE gene was monitored in lake-water microcosms. Various designs of microcosms were compared. These ranged from 250-ml conical flasks containing 100 ml surface lake water to 12-1 glass containers with lake water overlying sediment, continuous aeration and a supply of fresh surface lake water. The presence of a low flow-through rate was shown to have little effect on the survival of P. putida. An increase in the size of microcosm, presence of sediment and aeration had a significant effect on survival in lake water and increased the rate of decline of released cells. The implication of these results in predicting the survival of P. putida in lake water using microcosms is discussed.</p>","PeriodicalId":77262,"journal":{"name":"Microbial releases : viruses, bacteria, fungi","volume":"1 3","pages":"155-9"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12516156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Non-culturable Aeromonas salmonicida in lake water. 湖水中不可培养的杀沙门氏气单胞菌。
Microbial releases : viruses, bacteria, fungi Pub Date : 1992-10-01
J A Morgan, K J Clarke, G Rhodes, R W Pickup
{"title":"Non-culturable Aeromonas salmonicida in lake water.","authors":"J A Morgan,&nbsp;K J Clarke,&nbsp;G Rhodes,&nbsp;R W Pickup","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The survival of Aeromonas salmonicida subsp. salmonicida was investigated in lake water. During a 21-day study A. salmonicida became non-culturable in sterile lake water held at 10 degrees C. The incubation of replicate samples between 5 degrees C and 25 degrees C produced similar results. The recovery of colony-forming units of A. salmonicida from different lake water systems indicated that they survived longer in water that was naturally enriched (eutrophic) or enriched with tryptone soya broth. Flow cytometry, fluorescence light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) indicated that non-culturable cells were present. These cells could not be revived in dilutions of tryptone soya broth, whole dead fish or dissected fish tissue. Although viability could not be proven, it was shown that the morphological integrity found in viable cells was also maintained in non-culturable cells.</p>","PeriodicalId":77262,"journal":{"name":"Microbial releases : viruses, bacteria, fungi","volume":"1 2","pages":"71-8"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12516155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioluminescence-based detection of genetically engineered microorganisms in nonsterile river water. 非无菌河水中基因工程微生物的生物荧光检测。
Microbial releases : viruses, bacteria, fungi Pub Date : 1992-06-01
S Heller, S Bühler, S Kilz, M Mieschendahl
{"title":"Bioluminescence-based detection of genetically engineered microorganisms in nonsterile river water.","authors":"S Heller,&nbsp;S Bühler,&nbsp;S Kilz,&nbsp;M Mieschendahl","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The luminescence genes of the marine bacterium Vibrio fischeri were cloned into a lac expression vector and introduced into Escherichia coli and Pseudomonas putida. Survival of the cells in river water samples was monitored by light measurements. Whereas E. coli survived in sterilized river water for more than 29 days, it died off in nonsterile river water after 9 to 13 days. The engineered P. putida cells survived in nonsterile river water for more than 137 days. The detection limit for E. coli was 11 cells/ml.</p>","PeriodicalId":77262,"journal":{"name":"Microbial releases : viruses, bacteria, fungi","volume":"1 1","pages":"35-9"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12515321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DNA fingerprints of Pseudomonas spp. using rotating field electrophoresis. 假单胞菌旋转场电泳的DNA指纹图谱。
Microbial releases : viruses, bacteria, fungi Pub Date : 1992-06-01
H Claus, H Rötlich, Z Filip
{"title":"DNA fingerprints of Pseudomonas spp. using rotating field electrophoresis.","authors":"H Claus,&nbsp;H Rötlich,&nbsp;Z Filip","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Rotating field electrophoresis (RFE) was applied to evaluate the usefulness of this technique for identification of several Pseudomonas strains with suspected importance in deliberate releases. Genomes of common wild-type or genetically modified strains of Pseudomonas fluorescens, Pseudomonas stutzeri, Pseudomonas putida and Pseudomonas aeruginosa were digested with rare-cutting restriction endonucleases and subjected to pulsed field gel electrophoresis. Restrictions with SpeI or XbaI produced 11-28 large DNA fragments in the range of 50-500 kb pairs. The specific genomic fingerprints were different for most strains of the same species, but identical for closely related strains. Differences were not affected by the presence of natural or genetically modified plasmids.</p>","PeriodicalId":77262,"journal":{"name":"Microbial releases : viruses, bacteria, fungi","volume":"1 1","pages":"11-6"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12538125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mobilization of nonconjugative pBR322-derivative plasmids from laboratory strains of Escherichia coli to bacteria isolated from seawater. 大肠杆菌实验室菌株非共轭pbr322衍生物质粒向海水分离细菌的动员
Microbial releases : viruses, bacteria, fungi Pub Date : 1992-06-01
S J Sørensen
{"title":"Mobilization of nonconjugative pBR322-derivative plasmids from laboratory strains of Escherichia coli to bacteria isolated from seawater.","authors":"S J Sørensen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Mobilization of derivatives of plasmids pBR322 and pBR325 was shown to occur between Escherichia coli K12 strains in LB-broth at 37 degrees C, provided a mobilizer plasmid (F') was present either together with the nonconjugative plasmid or in a second donor strain. Evidence from restriction endonuclease analysis suggested that the mobilization was facilitated by a transposition phenomenon involving the \"gamma-delta\" sequence of F'. It was shown that mobilization of a derivative of pBR325 from E. coli K12 to bacteria isolated from seawater occurred in incubations in both LB-broth and filtered seawater and that Pseudomonas sp., Enterobacter aerogenes, Klebsiella oxytoca, E. coli, and Citrobacter amalonaticus isolates were recipient-active.</p>","PeriodicalId":77262,"journal":{"name":"Microbial releases : viruses, bacteria, fungi","volume":"1 1","pages":"17-22"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12514702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
OmpC and OmpF porins influence viability and culturability of Escherichia coli cells incubated in seawater. OmpC和OmpF孔蛋白影响大肠杆菌细胞在海水中培养的活力和培养能力。
Microbial releases : viruses, bacteria, fungi Pub Date : 1992-06-01
M J Gauthier, S A Benson, G N Flatau, R L Clement, V A Breittmayer, P M Munro
{"title":"OmpC and OmpF porins influence viability and culturability of Escherichia coli cells incubated in seawater.","authors":"M J Gauthier,&nbsp;S A Benson,&nbsp;G N Flatau,&nbsp;R L Clement,&nbsp;V A Breittmayer,&nbsp;P M Munro","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The contribution of the major outer membrane porins OmpF and OmpC to the maintenance of viability and culturability of Escherichia coli cells in seawater was analyzed using isogenic mutant strains lacking one or both porins. Cells that possessed OmpF and OmpC survived better than those lacking one or both of them. However, the results differed, depending on whether the cells were adapted to high osmolarity or not before transfer to seawater. When cells were grown at low osmolarity, survival was largely influenced by porins, the OmpF+ strains surviving better than those lacking this porin. Addition of an OmpF plasmid to OmpF- OmpC- cells also improved their viability. When grown at high osmolarity, the role of porins was less critical since both the viability and culturability of the cells increased. However, cells that expressed only OmpC showed the most dramatic loss of viability. Cells lacking both OmpF and OmpC exhibited a higher loss of viability and culturability in seawater. Regarding the influence of porins on survival, these results show that the conditions that prevail during the growth of cells before their transfer to seawater are highly influential: cells that express the porin corresponding to the growth conditions they are in at the time of transfer survive better.</p>","PeriodicalId":77262,"journal":{"name":"Microbial releases : viruses, bacteria, fungi","volume":"1 1","pages":"47-50"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12515322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Direct recovery and molecular analysis of DNA and RNA from soil. 土壤中DNA和RNA的直接回收和分子分析。
Microbial releases : viruses, bacteria, fungi Pub Date : 1992-06-01
S Selenska, W Klingmüller
{"title":"Direct recovery and molecular analysis of DNA and RNA from soil.","authors":"S Selenska,&nbsp;W Klingmüller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A simple method for the recovery of DNA and direct detection of nif and Tn5 sequences in soil has recently been presented by Selenska and Klingmüller. On the basis of that method we have developed a procedure for the recovery and separation of DNA and RNA from the same soil sample. A 550 bp sequence from the Kmr gene of Tn5 was identified by PCR amplifications in total DNA and RNA, isolated from soil inoculated with a nitrogen-fixing Enterobacter agglomerans 19-1-1, which carries this transposon. Hence, not only the presence of the Kmr gene of Tn5 but also its expression (mRNA synthesis) in the analyzed environmental samples was detected. The authenticity of the products of PCR amplifications of DNA and mRNA was confirmed by the PhotoGene hybridization technique.</p>","PeriodicalId":77262,"journal":{"name":"Microbial releases : viruses, bacteria, fungi","volume":"1 1","pages":"41-6"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12459188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Behavior in agricultural soils of a recombinant Pseudomonas bacterium that simultaneously degrades alkyl- and haloaromatics. 重组假单胞菌在农业土壤中的行为，同时降解烷基和卤代芳烃。
Microbial releases : viruses, bacteria, fungi Pub Date : 1992-06-01
A Delgado, E Duque, J L Ramos
{"title":"Behavior in agricultural soils of a recombinant Pseudomonas bacterium that simultaneously degrades alkyl- and haloaromatics.","authors":"A Delgado,&nbsp;E Duque,&nbsp;J L Ramos","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pseudomonas sp. FR1 (pFRC20P) is a recombinant bacterium able simultaneously to degrade alkyl- and haloaromatics due to the xylXYZL and xylS genes from the TOL plasmid borne on the bacterial chromosome, and to the gene encoding for a gamma-lactone isomerase from Alcaligenes eutrophus on pFRC20P. The survival of this strain in sterile soils was shown to depend on the physicochemical properties of the soil. The recombinant information was stable in bacteria introduced in soils and conferred selective advantage to the host bacterium when the soils were amended with low amounts of p-methylbenzoate. However, relatively high amounts of this chemical significantly reduced survival of the bacterium. Survival was more seriously affected by chlorobenzoates than by methylbenzoates. Evolution of 14CO2 from p-methyl-14C-benzoate in soils confirmed that Pseudomonas sp. FR1 (pFRC20P) functions in a complex system in accordance with its design. Survival of Pseudomonas sp. FR1 (pFRC20P) was better at 4 degrees C and 25 degrees C than at 37 degrees C. On plates, the pFRC20P plasmid was mobilized by helper plasmids to P. putida at a frequency of about 10(-6) transconjugants per recipient. However, no transfer of recombinant DNA integrated on the chromosome was found, nor was lateral transfer of the recombinant DNA borne by Pseudomonas sp. FR1 (pFRC20P) detected in soils.</p>","PeriodicalId":77262,"journal":{"name":"Microbial releases : viruses, bacteria, fungi","volume":"1 1","pages":"23-8"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12514703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of closely related Agrobacterium vitis isolates by chromosomal DNA probes. 用染色体DNA探针鉴定近缘葡萄农杆菌分离株。
Microbial releases : viruses, bacteria, fungi Pub Date : 1992-06-01
L Otten, J Canaday
{"title":"Identification of closely related Agrobacterium vitis isolates by chromosomal DNA probes.","authors":"L Otten,&nbsp;J Canaday","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Crown gall formation on grapevine by Agrobacterium vitis is an important plant disease in many regions of the world. On grapevine, octopine/cucumopine (o/c) strains are widely distributed. Here we describe two chromosomal sequences of o/c strains of 15 and 20 kb, which reveal a high degree of polymorphism in different o/c isolates. Part of the polymorphism is due to the presence or absence of a 2.2-kb-long repeated sequence, a homolog of which is also found on the octopine Ti plasmid of Ach5, immediately to the left of the left TL-region border. The occurrence and distribution of this repeat in different o/c isolates make it possible to reconstruct the evolution of these strains. The chromosomal DNA sequences outside the repeats also differ in various isolates. The two probes described here can be used to identify strains from a collection, classify new isolates, or trace a given isolate under experimental release conditions.</p>","PeriodicalId":77262,"journal":{"name":"Microbial releases : viruses, bacteria, fungi","volume":"1 1","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12515320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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