Human antibodies and hybridomas最新文献_第6页

Antigen-specific primary immune response of human B-lymphocytes after in vitro immunization with GM3 ganglioside. GM3神经节苷脂体外免疫后人b淋巴细胞抗原特异性初级免疫应答的研究。

Human antibodies and hybridomas Pub Date : 1995-01-01

M Alfonso, B Lanne, P Ifversen, A M Vázquez, R Pérez, J Portoukalian, J Zeuthen

{"title":"Antigen-specific primary immune response of human B-lymphocytes after in vitro immunization with GM3 ganglioside.","authors":"M Alfonso, B Lanne, P Ifversen, A M Vázquez, R Pérez, J Portoukalian, J Zeuthen","doi":"","DOIUrl":"","url":null,"abstract":"In vitro immunization of human B-lymphocytes was performed with liposomes containing the monosialoganglioside GM3, with or without either complete tetanus toxoid or a synthetic T helper epitope derived from tetanus toxin (determinant 830-843). The immunized B-cells were Epstein-Barr virus transformed and the human anti-ganglioside antibody response was evaluated using an indirect ELISA against different mono- and disialogangliosides. Clones producing antigen-specific human antibodies of the IgM isotype against the ganglioside GM3 used as the immunogen were selected and one clone, IM-11, was further characterized. In addition, a method of positive selection using GM3-coated magnetic beads has been developed which allowed us to rescue unstable clones. The binding of the human antibody IM-11 to a large panel of glycosphingolipids separated on thin-layer plates was studied. The human MAb IM-11 was found to bind strongly to NeuAcGM3, IV3 NeuAcnLc4 and sulfate containing glycosphingolipids and weakly to NeuGcGM3. Immunohistological staining of melanoma and breast cancer biopsy sections showed a selective reactivity of IM-11 with tumor cells which varied among different tumors.","PeriodicalId":77166,"journal":{"name":"Human antibodies and hybridomas","volume":"6 3","pages":"102-12"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19577151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A genetically engineered single-gene-encoded anti-TAG72 chimeric antibody secreted from myeloma cells. 一种由骨髓瘤细胞分泌的基因工程单基因编码抗tag72嵌合抗体。

Human antibodies and hybridomas Pub Date : 1995-01-01

Y Qi, J Xiang

引用次数: 0

Efficient immortalization of rheumatoid synovial tissue B-lymphocytes. A comparison between the techniques of electric field-induced and PEG fusion. 类风湿性滑膜组织b淋巴细胞的高效永生化。电场诱导与聚乙二醇融合技术之比较。

Human antibodies and hybridomas Pub Date : 1995-01-01

V Krenn, P von Landenberg, E Wozniak, C Kissler, H K Hermelink, U Zimmermann, H P Vollmers

{"title":"Efficient immortalization of rheumatoid synovial tissue B-lymphocytes. A comparison between the techniques of electric field-induced and PEG fusion.","authors":"V Krenn, P von Landenberg, E Wozniak, C Kissler, H K Hermelink, U Zimmermann, H P Vollmers","doi":"","DOIUrl":"","url":null,"abstract":"In this study, B-cells isolated from rheumatoid synovial tissue were immortalized, without prior in vitro stimulation, by means of electric-field induced fusion and conventional PEG fusion in order to compare the efficiency of these methods. Two myeloma cell lines were used as fusion partners, the murine myeloma Ag8 and the murine-human heteromyeloma HAB-1. The results of seven fusion experiments performed simultaneously with identical cell populations showed that fusion frequencies obtained by electrofusion were 4 to 35 times higher than by the PEG fusion technique. The morphological and immunohistochemical evaluation of synovial tissues used for fusion showed that only tissues exhibiting a follicular distribution of B-cells with a high percentage of CD 22-positive lymphocytes gave rise to high fusion yields and produced B-cell clones, whereas synovial tissues with the same percentage of plasma cells but lower percentages of CD 22 lymphocytes yielded very low fusion rates. In conclusion, electrofusion is more efficient for immortalizing small amounts of synovial tissue B-lymphocytes than PEG fusion, since high fusion frequencies could be obtained by this technique without the need for prior in vitro stimulation. Synovial tissue exhibiting a follicular distribution of B-lymphocytes with high percentages of CD 22-positive lymphocytes gave rise to high hybridoma yields and therefore an ideal source of human rheumatoid B-cell clones.","PeriodicalId":77166,"journal":{"name":"Human antibodies and hybridomas","volume":"6 2","pages":"47-51"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18500910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macrophage subpopulations in the thymic hyperplasia of patients with myasthenia gravis. 巨噬细胞亚群在重症肌无力患者胸腺增生中的作用。

Human antibodies and hybridomas Pub Date : 1995-01-01 DOI: 10.3233/HAB-1995-6405

Jian Zhang, S. Cohen-Kaminsky, Sonia Berrih-Aknin

{"title":"Macrophage subpopulations in the thymic hyperplasia of patients with myasthenia gravis.","authors":"Jian Zhang, S. Cohen-Kaminsky, Sonia Berrih-Aknin","doi":"10.3233/HAB-1995-6405","DOIUrl":"https://doi.org/10.3233/HAB-1995-6405","url":null,"abstract":"Macrophages were studied in sections of the thymus from patients with Myasthenia Gravis, using a panel of monoclonal antibodies against the monocyte/macrophage lineage. In normal areas, CD14 and CD35 were preferentially expressed in the medulla while RM3/1 and 25F9 markers stained essentially cortical macrophages. CD11c-labelled cells were densely located throughout the thymus. The antibody 27E10 recognized clusters of cells located around or in the perivascular areas, that could be migrating monocytes. In the germinal centers, cells were stained with CD14, CD35, CD11c and 25F9 but not with RM3/1 and 27E10 markers. The numbers of CD14- and CD35-positive cells were significantly increased in Myasthenia Gravis thymic hyperplasia compared to control thymus (p < 0.025 and p < 0.01, respectively). This increase was clearly due to the higher number of positive cells in the germinal centers and in the areas surrounding the lymphoid follicles. The potential role of these cells in accessory cell function and antigen presentation could be relevant in terms of intrathymic sensitization and autoantibody production that have been demonstrated in thymic hyperplasia from Myasthenia Gravis patients.","PeriodicalId":77166,"journal":{"name":"Human antibodies and hybridomas","volume":"6 4 1","pages":"153-60"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/HAB-1995-6405","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69905953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A genetically engineered single-gene-encoded anti-TAG72 chimeric antibody secreted from myeloma cells. 一种由骨髓瘤细胞分泌的基因工程单基因编码抗tag72嵌合抗体。

Human antibodies and hybridomas Pub Date : 1995-01-01 DOI: 10.3233/HAB-1995-6406

Y. Qi, J. Xiang

引用次数: 1

Immortalization of plasma cells by plasmid DNA and its hybridoma. 质粒DNA对浆细胞的永生化及其杂交瘤。

Human antibodies and hybridomas Pub Date : 1995-01-01 DOI: 10.3233/HAB-1995-6302

T. Kanki, S. Takeuchi

引用次数: 2

4th International Conference on Human Antibodies and Hybridomas. Amsterdam, The Netherlands, 26-28 April 1995. Abstracts. 第四届国际人抗体和杂交瘤会议。1995年4月26日至28日，荷兰阿姆斯特丹。摘要。

Human antibodies and hybridomas Pub Date : 1995-01-01

引用次数: 0

Dual recognition of lipid A and DNA by human antibodies encoded by the VH4-21 gene: a possible link between infection and lupus. 由VH4-21基因编码的人抗体对脂质A和DNA的双重识别:感染与狼疮之间的可能联系。

Human antibodies and hybridomas Pub Date : 1995-01-01

M B Spellerberg, C J Chapman, C I Mockridge, D A Isenberg, F K Stevenson

{"title":"Dual recognition of lipid A and DNA by human antibodies encoded by the VH4-21 gene: a possible link between infection and lupus.","authors":"M B Spellerberg, C J Chapman, C I Mockridge, D A Isenberg, F K Stevenson","doi":"","DOIUrl":"","url":null,"abstract":"The VH4-21 (V4-34) gene segment, a member of the VH4 family, is expressed early in B-cell maturation and is utilized by approximately 6% of normal adult B lymphocytes. This prevalence indicates an importance of VH4-21 in the B-cell repertoire. The gene also encodes certain autoantibodies being mandatory for pathological IgM anti-red cell antibodies directed against the I/i antigen, and also capable of encoding anti-DNA antibodies. Recognition of I/i antigen or DNA appears to be via two distinct sites on VH, with I/i binding mediated by sequences in the framework region, and DNA binding correlating with the presence of positively charged amino acids in complementarity-determining region 3. However, these positively charged residues appear to suppress the ability of the framework region to interact with I/i, rendering a single sequence monospecific for I/i or DNA. The IgM anti-DNA antibodies also recognize bacterial lipid A, whereas the anti-I/i antibodies do not, indicating that CDR3 may be involved in binding the negatively charged lipid A. Structural similarities between the DNA backbone and lipid A provide a possible explanation for this cross-reactivity. This dual recognition of bacterial antigen and autoantigen provides a potential link between infection and autoimmunity.","PeriodicalId":77166,"journal":{"name":"Human antibodies and hybridomas","volume":"6 2","pages":"52-6"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18500911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A human monoclonal antibody to carbohydrate moiety of carcinoembryonic antigen. 人抗癌胚抗原碳水化合物部分的单克隆抗体。

Human antibodies and hybridomas Pub Date : 1995-01-01 DOI: 10.3233/HAB-1995-6404

K. Tsukazaki, K. Kuroda, K. Mochizuki, K. Kubushiro, T. Fukuchi, M. Kato, S. Hashizume, S. Nozawa

{"title":"A human monoclonal antibody to carbohydrate moiety of carcinoembryonic antigen.","authors":"K. Tsukazaki, K. Kuroda, K. Mochizuki, K. Kubushiro, T. Fukuchi, M. Kato, S. Hashizume, S. Nozawa","doi":"10.3233/HAB-1995-6404","DOIUrl":"https://doi.org/10.3233/HAB-1995-6404","url":null,"abstract":"Hybridoma BSRF-S-97, secreting a human monoclonal antibody of IgG1 subclass reactive to the carcinoembryonic antigen, was generated by fusing the regional lymph node lymphocytes from a cervical cancer patient with RF-S1 human-mouse heteromyeloma fusion line. This monoclonal antibody was found specifically reactive to carinoembryonic antigen-producing cell lines, including those of cervical cancer (SKG-II), mucinous type ovarian cancer (RMUG-L), stomach cancer (MKN-45), and lung cancer (PC-10). The monoclonal antibody reactivity with pepsin- and periodate-treated carcinoembryonic antigen demonstrated that this monoclonal antibody recognizes the carbohydrate moiety of carcinoembryonic antigen specifically. Possibilities of the monoclonal antibody reaction with mucin and blood-group antigens were excluded by the comparative studies with a placental mucin-containing protein which reacted with carcinoembryonic antigen-specific rabbit polyclonal antibody. The monoclonal antibody conjugated with Pseudomonas exotoxin showed potent regression effects on the growth of the MKN-45 cell line in both the dish culture and xenografted nude mice, indicating potential usefulness of this human monoclonal antibody as a promising tumor targeting vehicle.","PeriodicalId":77166,"journal":{"name":"Human antibodies and hybridomas","volume":"6 4 1","pages":"145-52"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/HAB-1995-6404","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69905945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparison of anti-TNF alpha autoantibodies in plasma and from EBV transformed lymphocytes of autoimmune and normal individuals. 自身免疫与正常人血浆及EBV转化淋巴细胞抗tnf α自身抗体的比较。

Human antibodies and hybridomas Pub Date : 1995-01-01 DOI: 10.3233/HAB-1995-6206

L. Tsuchiyama, T. Wong, J. Kieran, P. Boyle, D. Penza, G. D. Wetzel

{"title":"Comparison of anti-TNF alpha autoantibodies in plasma and from EBV transformed lymphocytes of autoimmune and normal individuals.","authors":"L. Tsuchiyama, T. Wong, J. Kieran, P. Boyle, D. Penza, G. D. Wetzel","doi":"10.3233/HAB-1995-6206","DOIUrl":"https://doi.org/10.3233/HAB-1995-6206","url":null,"abstract":"To examine the ability of normal and autoimmune individuals to produce circulating anti-TNF alpha antibodies, plasma samples from 10 RA patients, 10 SLE patients and 5 normal subjects were assessed for anti-TNF alpha antibody. While every individual tested demonstrated circulating IgM anti-TNF alpha antibody, IgG anti-TNF alpha autoantibody was seen predominantly in autoimmune patients. Only 1 of 5 normal individuals, but 15 of 20 autoimmune individuals had plasma IgG anti-TNF alpha antibodies. To examine the ability of normal and autoimmune individuals to produce anti-TNF alpha autoantibody from their circulating lymphocytes, EBV transformation was performed. Oligoclonal immortal cell lines were successfully established from 13 patients and each one secreted detectable IgM anti-TNF alpha autoantibody. Transformed cells from only 1 of 5 normal individuals secreted IgM anti-TNF alpha autoantibody. These results indicate a higher prevalence of anti-TNF alpha autoantibody production among autoimmune individuals although normal individuals are also capable of producing these autoantibodies.","PeriodicalId":77166,"journal":{"name":"Human antibodies and hybridomas","volume":"6 2 1","pages":"73-6"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69906126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4